| GCATTGC-------------------CT-----------------------------------------------------------------------------TAACGTTATTA---------T-TATT----------T-A-----------------------------------AATAATGCTTAGAAATAG-----------------------------------TAATAGCCGAA--------------------TCTATG--CACGTC---------------------------------------------------------------------------------------------------------------------------------------------ACAAAATAAATTTCGAA------------AATTACT-----TTATAT-----------TAGAAT | Size | Hit Count | Total Norm | Total | M023 Head | M024 Male-body | M025 Embryo | M053 Female-body | O001 Testis | O002 Head | SRR553485 Ovary | SRR553486 Ovary | SRR553487 Ovary | SRR553488 Ovary | SRR618934 Ovary | V044 Embryo | SRR902008 Ovary | SRR902009 Testis | SRR1275487 Male larvae | SRR1275485 Male prepupae | SRR1275483 Male prepupae |
| .........................................................................................................................................................................................................................................................................TATG--CACGTC---------------------------------------------------------------------------------------------------------------------------------------------ACAAAATAAATTTCGA................................................ | 26 | 1 | 3.00 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ........................................................................................................................................................................................................................................TAATAGCCGAA--------------------TCTATG--CACGTC---------------------------------------------------------------------------------------------------------------------------------------------ACA............................................................. | 26 | 1 | 3.00 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................TTAGAAATAG-----------------------------------TAATAGCCGAA--------------------TCT........................................................................................................................................................................................................................ | 24 | 20 | 2.00 | 40 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | 0 | 15 | 0 | 0 | 0 | 13 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................TG--CACGTC---------------------------------------------------------------------------------------------------------------------------------------------ACAAAATAAATTTCGA................................................ | 24 | 1 | 2.00 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................TTAGAAATAG-----------------------------------TAATAGCCGAA--------------------TCTA....................................................................................................................................................................................................................... | 25 | 20 | 1.60 | 32 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 11 | 0 | 0 | 0 | 0 | 15 | 5 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................TG--CACGTC---------------------------------------------------------------------------------------------------------------------------------------------ACAAAATAAATTTCGAA------------AATT............................... | 29 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ........................................................................................................................................................................................................................................TAATAGCCGAA--------------------TCTATG--CACGTC---------------------------------------------------------------------------------------------------------------------------------------------A............................................................... | 24 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ........................................................................................................................................................................................................................................TAATAGCCGAA--------------------TCTATG--CACGTC---------------------------------------------------------------------------------------------------------------------------------------------AC.............................................................. | 25 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................................................................................................TG--CACGTC---------------------------------------------------------------------------------------------------------------------------------------------ACAAAATAAATTTCGAA------------AAT................................ | 28 | 1 | 1.00 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................................................................................G-----------------------------------TAATAGCCGAA--------------------TCTATG--................................................................................................................................................................................................................... | 18 | 20 | 0.40 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................TTAGAAATAG-----------------------------------TAATAGCCGAA--------------------T.......................................................................................................................................................................................................................... | 22 | 20 | 0.40 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 4 | 0 | 0 | 0 |
| ........................................................................................................................................................................................................................................TAATAGCCGAA--------------------TCTATG--................................................................................................................................................................................................................... | 17 | 20 | 0.35 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................................................AGAAATAG-----------------------------------TAATAGCCGAA--------------------TCTAT...................................................................................................................................................................................................................... | 24 | 20 | 0.30 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................TTAGAAATAG-----------------------------------TAATAGCCGAA--------------------TC......................................................................................................................................................................................................................... | 23 | 20 | 0.25 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 0 |
| ........................................................................................................................................................................................TGCTTAGAAATAG-----------------------------------TAATAGCCGAA--------------------........................................................................................................................................................................................................................... | 24 | 4 | 0.25 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................TTAGAAATAG-----------------------------------TAATAGCCGAA--------------------........................................................................................................................................................................................................................... | 21 | 20 | 0.25 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
| ..................................................................................................................................................................................................TAG-----------------------------------TAATAGCCGAA--------------------TCT........................................................................................................................................................................................................................ | 17 | 20 | 0.20 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
| ........................................................................................................................................................................................................................................TAATAGCCGAA--------------------TCTA....................................................................................................................................................................................................................... | 15 | 20 | 0.20 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...TTGC-------------------CT-----------------------------------------------------------------------------TAACGTTATTA---------T-TATT----------..................................................................................................................................................................................................................................................................................................................................................... | 22 | 20 | 0.15 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 |
| ...............................................................................................................................................................................................AAATAG-----------------------------------TAATAGCCGA................................................................................................................................................................................................................................................ | 16 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| .................................................................................................................................................................................................ATAG-----------------------------------TAATAGCCGAA--------------------TCTA....................................................................................................................................................................................................................... | 19 | 20 | 0.10 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| ..................................................................................................................................................................................................................................................................................................................................................................................................................................ACAAAATAAATTTCGA................................................ | 16 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................TTAGAAATAG-----------------------------------TAATAGCCGT................................................................................................................................................................................................................................................ | 20 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| .............................................................................................................................................................................................AGAAATAG-----------------------------------TAATAGCCG................................................................................................................................................................................................................................................. | 17 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ....................................................................................................................................................................................................................................................................................C---------------------------------------------------------------------------------------------------------------------------------------------ACAAAATAAATTTCGAA------------AA................................. | 20 | 20 | 0.05 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................TTAGAAATAG-----------------------------------TAATA..................................................................................................................................................................................................................................................... | 15 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................TAGAAATAG-----------------------------------TAATAGCCGAA--------------------TCTATG--................................................................................................................................................................................................................... | 26 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................TTAGAAATAG-----------------------------------TAATAGCCGAA--------------------TCTT....................................................................................................................................................................................................................... | 25 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
| ...........................T-----------------------------------------------------------------------------TAACGTTATTA---------T-TA................................................................................................................................................................................................................................................................................................................................................................. | 15 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................TAGAAATAG-----------------------------------TAATAGCCGA................................................................................................................................................................................................................................................ | 19 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ............................................................................................................................................................................................TAGAAATAG-----------------------------------TAATAGCCGAA--------------------TCT........................................................................................................................................................................................................................ | 23 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................TTAGAAATAG-----------------------------------TAATAGCCGAA--------------------TCTAC...................................................................................................................................................................................................................... | 26 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
| ..................................................................................................................................................................................................TAG-----------------------------------TAATAGCCGAA--------------------TCTAT...................................................................................................................................................................................................................... | 19 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................TTAGAAATAG-----------------------------------TAATAGCCGAA--------------------TCTAT...................................................................................................................................................................................................................... | 26 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
| ..................................................................................................................................................................................................TAG-----------------------------------TAATAGCCGAA--------------------TCTATG--................................................................................................................................................................................................................... | 20 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| ...........................................................................................................................................................................................TTAGAAATAG-----------------------------------TAATAGCCGAA--------------------TA......................................................................................................................................................................................................................... | 23 | 20 | 0.05 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |