dmo_787

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------################################################## TCAGATCTATTCCCTATCAGTATGGACGCACTATAATATTAAATTGATTGCAAATTGTCTATTGATTAGGATTGTAGTGTAAGACTTCATGATAAGAAAATATCCAAGTGGATTTCACGGACATATTGCACATTGGGCTATGATCGTATTCCTGCCAGTTTTAGATTCAATGCAATTTAATTGATGCATCCTCTTCCACAGCTCAATTTAAGTGTGAATAAAAAGCGGAGCCAAACGGAAAATACATCGGA ************************************************....((...(((((((((.(((((((((.(((((((.((.((...((..(((((((((((..................))).))))).)))..))....)).))..))))))).))....)))))))))))))))).............))************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M046 female body	V110 male body	V056 head
............................................................................................................................................................ATTTTTAGATTCAATGCAAT...........................................................................	20	1	1	25.00	25	18	6	1
............................................................................................................................................................ATTTTTAGATTCAATGCAATT..........................................................................	21	1	1	12.00	12	11	1	0
.............................................................................................................................................................TTTTTAGATTCAATGCAAT...........................................................................	19	1	1	7.00	7	7	0	0
...........................................................................................................................................................TATTTTTAGATTCAATGCAAT...........................................................................	21	2	2	5.00	10	8	1	1
..............................................................................................................................................................TTTTAGATTCAATGCAATT..........................................................................	19	0	1	3.00	3	3	0	0
..............................................................................................................................................................TTTTAGATTCAATGCAAT...........................................................................	18	0	1	2.00	2	2	0	0
............................................................................................................................................................ATTTTTAGATTCAATGCAA............................................................................	19	1	1	2.00	2	2	0	0
.............................................................................................................................................................TTTTTAGATTCAATGCAATT..........................................................................	20	1	1	2.00	2	2	0	0
...........................................................................................................................................................TATTTTTAGATTCAATGCAATT..........................................................................	22	2	2	1.50	3	3	0	0
..............................................................................................................................................................TTTTAGATTCAATGCAATTA.........................................................................	20	1	1	1.00	1	1	0	0
..............................................................................................................................................................TTTTAGATTCAATGCAATTG.........................................................................	20	1	1	1.00	1	1	0	0
..............................................................................................................................................................TTTTAGATTCAATGCAATTAC........................................................................	21	2	2	0.50	1	1	0	0
............................................................................................................................................................ATTTTTAGATTCAATGCAATTA.........................................................................	22	2	2	0.50	1	1	0	0
...........................................................................................................................................................TATTTTTAGATTCAATGCAA............................................................................	20	2	5	0.40	2	1	0	1
..........................................................................................................................................................TTATTTTTAGATTCAATGCAAT...........................................................................	22	3	19	0.37	7	4	3	0
............................................................................................................................................................ATTTTTAGATTCAATGCAATTAC........................................................................	23	3	4	0.25	1	1	0	0
...............................................................................................................................................................TTTAGATTCAATGCAATTAC........................................................................	20	2	4	0.25	1	1	0	0
..........................................................................................................................................................TTATTTTTAGATTCAATGCAATT..........................................................................	23	3	12	0.17	2	2	0	0
...........................................................................................................................................................TATTTTTAGATTCAATGCA.............................................................................	19	2	20	0.15	3	3	0	0
................................................................................................................................................................TTAGATTCAATGCAATTAC........................................................................	19	2	7	0.14	1	1	0	0
..........................................................................................................................................................TTATTTTTAGATTCAATGCAA............................................................................	21	3	20	0.10	2	2	0	0
.....................................................ATTGTCTAGTGATTAGGA....................................................................................................................................................................................	18	1	12	0.08	1	1	0	0

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------##################################################
TCAGATCTATTCCCTATCAGTATGGACGCACTATAATATTAAATTGATTGCAAATTGTCTATTGATTAGGATTGTAGTGTAAGACTTCATGATAAGAAAATATCCAAGTGGATTTCACGGACATATTGCACATTGGGCTATGATCGTATTCCTGCCAGTTTTAGATTCAATGCAATTTAATTGATGCATCCTCTTCCACAGCTCAATTTAAGTGTGAATAAAAAGCGGAGCCAAACGGAAAATACATCGGA
**************************************************....((...(((((((((.(((((((((.(((((((.((.((...((..(((((((((((..................))).))))).)))..))....)).))..))))))).))....)))))))))))))))).............))**************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M046
female body

V110
male body

V056
head

............................................................................................................................................................ATTTTTAGATTCAATGCAAT...........................................................................

25.00

............................................................................................................................................................ATTTTTAGATTCAATGCAATT..........................................................................

12.00

.............................................................................................................................................................TTTTTAGATTCAATGCAAT...........................................................................

7.00

...........................................................................................................................................................TATTTTTAGATTCAATGCAAT...........................................................................

5.00

..............................................................................................................................................................TTTTAGATTCAATGCAATT..........................................................................

3.00

..............................................................................................................................................................TTTTAGATTCAATGCAAT...........................................................................

2.00

............................................................................................................................................................ATTTTTAGATTCAATGCAA............................................................................

2.00

.............................................................................................................................................................TTTTTAGATTCAATGCAATT..........................................................................

2.00

...........................................................................................................................................................TATTTTTAGATTCAATGCAATT..........................................................................

1.50

..............................................................................................................................................................TTTTAGATTCAATGCAATTA.........................................................................

1.00

..............................................................................................................................................................TTTTAGATTCAATGCAATTG.........................................................................

1.00

..............................................................................................................................................................TTTTAGATTCAATGCAATTAC........................................................................

0.50

............................................................................................................................................................ATTTTTAGATTCAATGCAATTA.........................................................................

0.50

...........................................................................................................................................................TATTTTTAGATTCAATGCAA............................................................................

0.40

..........................................................................................................................................................TTATTTTTAGATTCAATGCAAT...........................................................................

0.37

............................................................................................................................................................ATTTTTAGATTCAATGCAATTAC........................................................................

0.25

...............................................................................................................................................................TTTAGATTCAATGCAATTAC........................................................................

0.25

..........................................................................................................................................................TTATTTTTAGATTCAATGCAATT..........................................................................

0.17

...........................................................................................................................................................TATTTTTAGATTCAATGCA.............................................................................

0.15

................................................................................................................................................................TTAGATTCAATGCAATTAC........................................................................

0.14

..........................................................................................................................................................TTATTTTTAGATTCAATGCAA............................................................................

0.10

.....................................................ATTGTCTAGTGATTAGGA....................................................................................................................................................................................

0.08

AGTCTAGATAAGGGATAGTCATACCTGCGTGATATTATAATTTAACTAACGTTTAACAGATAACTAATCCTAACATCACATTCTGAAGTACTATTCTTTTATAGGTTCACCTAAAGTGCCTGTATAACGTGTAACCCGATACTAGCATAAGGACGGTCAAAATCTAAGTTACGTTAAATTAACTACGTAGGAGAAGGTGTCGAGTTAAATTCACACTTATTTTTCGCCTCGGTTTGCCTTTTATGTAGCCT ************************************************....((...(((((((((.(((((((((.(((((((.((.((...((..(((((((((((..................))).))))).)))..))....)).))..))))))).))....)))))))))))))))).............))************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M046 female body	V110 male body	V056 head	M060 embryo
..............................................................................................................................................................AAAATCTAAGTTACGTTAA..........................................................................	19	0	1	17.00	17	15	2	0	0
............................................................................................................................................................TAAAAATCTAAGTTACGTTAA..........................................................................	21	1	1	13.00	13	7	5	1	0
............................................................................................................................................................TAAAAATCTAAGTTACGTTA...........................................................................	20	1	1	4.00	4	4	0	0	0
...........................................................................................................................................................ATAAAAATCTAAGTTACGTTAA..........................................................................	22	2	2	3.50	7	6	1	0	0
..............................................................................................................................................................AAAATCTAAGTTACGTTAATG........................................................................	21	2	2	2.00	4	2	1	1	0
...........................................................................................................................................................ATAAAAATCTAAGTTACGTTA...........................................................................	21	2	2	1.50	3	3	0	0	0
...............................................................................................................................................................AAATCTAAGTTACGTTAATG........................................................................	20	2	4	1.00	4	3	1	0	0
..............................................................................................................................................................AAAATCTAAGTTACGTTAAT.........................................................................	20	1	1	1.00	1	0	1	0	0
.............................................................................................................................................................AAAAATCTAAGTTACGTTA...........................................................................	19	1	1	1.00	1	1	0	0	0
.............................................................................................................................................................AAAAATCTAAGTTACGTTAA..........................................................................	20	1	1	1.00	1	1	0	0	0
............................................................................................................................................................TAAAAATCTAAGTTACGTTAATG........................................................................	23	3	4	0.75	3	2	1	0	0
.................................................................................................................................................CACAAGGACGGCCAAAAT........................................................................................	18	2	2	0.50	1	0	0	0	1
..............................................................................................................................................................AAAATCTGAGTTACGTTAA..........................................................................	19	1	2	0.50	1	1	0	0	0
................................................................................................................................................................AATCTAAGTTACGTTAATG........................................................................	19	2	7	0.29	2	1	1	0	0
...............................................................................................................................................TGCATAGGGACGGGCAAAAT........................................................................................	20	3	6	0.17	1	0	0	1	0
..........................................................................................................................................................AATAAAAATCTAAGTTACGTTA...........................................................................	22	3	19	0.11	2	2	0	0	0
.................................................................................................................................................................ATCTAAGTTACGTTAATG........................................................................	18	2	20	0.05	1	1	0	0	0
..........................................................................................................................................................AATAAAAATCTAAGTTACGTT............................................................................	21	3	20	0.05	1	1	0	0	0

AGTCTAGATAAGGGATAGTCATACCTGCGTGATATTATAATTTAACTAACGTTTAACAGATAACTAATCCTAACATCACATTCTGAAGTACTATTCTTTTATAGGTTCACCTAAAGTGCCTGTATAACGTGTAACCCGATACTAGCATAAGGACGGTCAAAATCTAAGTTACGTTAAATTAACTACGTAGGAGAAGGTGTCGAGTTAAATTCACACTTATTTTTCGCCTCGGTTTGCCTTTTATGTAGCCT
**************************************************....((...(((((((((.(((((((((.(((((((.((.((...((..(((((((((((..................))).))))).)))..))....)).))..))))))).))....)))))))))))))))).............))**************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M046
female body

V110
male body

V056
head

M060
embryo

..............................................................................................................................................................AAAATCTAAGTTACGTTAA..........................................................................

17.00

............................................................................................................................................................TAAAAATCTAAGTTACGTTAA..........................................................................

13.00

............................................................................................................................................................TAAAAATCTAAGTTACGTTA...........................................................................

4.00

...........................................................................................................................................................ATAAAAATCTAAGTTACGTTAA..........................................................................

3.50

..............................................................................................................................................................AAAATCTAAGTTACGTTAATG........................................................................

2.00

...........................................................................................................................................................ATAAAAATCTAAGTTACGTTA...........................................................................

1.50

...............................................................................................................................................................AAATCTAAGTTACGTTAATG........................................................................

1.00

..............................................................................................................................................................AAAATCTAAGTTACGTTAAT.........................................................................

1.00

.............................................................................................................................................................AAAAATCTAAGTTACGTTA...........................................................................

1.00

.............................................................................................................................................................AAAAATCTAAGTTACGTTAA..........................................................................

1.00

............................................................................................................................................................TAAAAATCTAAGTTACGTTAATG........................................................................

0.75

.................................................................................................................................................CACAAGGACGGCCAAAAT........................................................................................

0.50

..............................................................................................................................................................AAAATCTGAGTTACGTTAA..........................................................................

0.50

................................................................................................................................................................AATCTAAGTTACGTTAATG........................................................................

0.29

...............................................................................................................................................TGCATAGGGACGGGCAAAAT........................................................................................

0.17

..........................................................................................................................................................AATAAAAATCTAAGTTACGTTA...........................................................................

0.11

.................................................................................................................................................................ATCTAAGTTACGTTAATG........................................................................

0.05

..........................................................................................................................................................AATAAAAATCTAAGTTACGTT............................................................................

0.05

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droMoj3	scaffold_6473:14442266-14442516 +	dmo_787	TCAGATCTATTCCCTATCAGTATGGACGCACTATAATATTAAATTGATTGCAAATTGTCTATTGATTAGGATTGTAGTGTAAGACTTCATGATAAGAAAATATCCAAGTGGATTTCACGGACATATTGCACATTGGGCTATGATCGTATTCCTGCCAGTTTTAGATTCAATGCAATTTAATTGATGCATCCTCTTCCACAGCTCAATTTAAGTGTGAATAAAAAGCGGAGCCAAACGG------AAAATACATCGGA
droVir3	scaffold_12928:2670917-2670975 +		CAGCAT------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GATTTGTCTTTGAACAAGAGTCACAACGAGTCGGAGCAGGAGGATATATCGGA
droGri2	scaffold_15110:6158464-6158475 -		-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGATAAGTCGGG
droWil2	scf2_1100000002375:7929-7951 +		CCAGATC-ACCCATTTTCCGTATG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droMoj3

TCAGATCTATTCCCTATCAGTATGGACGCACTATAATATTAAATTGATTGCAAATTGTCTATTGATTAGGATTGTAGTGTAAGACTTCATGATAAGAAAATATCCAAGTGGATTTCACGGACATATTGCACATTGGGCTATGATCGTATTCCTGCCAGTTTTAGATTCAATGCAATTTAATTGATGCATCCTCTTCCACAGCTCAATTTAAGTGTGAATAAAAAGCGGAGCCAAACGG------AAAATACATCGGA	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
..............................................................................................................................................................TTTTAGATTCAATGCAATT................................................................................	19	1	3.00	3	3	0	0	0	0	0
..............................................................................................................................................................TTTTAGATTCAATGCAAT.................................................................................	18	1	2.00	2	2	0	0	0	0	0
..............................................................................................................................................................TTTTAGATTCAATGCAATTA...............................................................................	20	1	1.00	1	1	0	0	0	0	0
..............................................................................................................................................................TTTTAGATTCAATGCAATTG...............................................................................	20	1	1.00	1	1	0	0	0	0	0
..............................................................................................................................................................TTTTAGATTCAATGCAATTAC..............................................................................	21	2	0.50	1	1	0	0	0	0	0
...............................................................................................................................................................TTTAGATTCAATGCAATTAC..............................................................................	20	4	0.25	1	1	0	0	0	0	0
................................................................................................................................................................TTAGATTCAATGCAATTAC..............................................................................	19	7	0.14	1	1	0	0	0	0	0

droVir3

CAGCAT------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GATTTGTCTTTGAACAAGAGTCACAACGAGTCGGAGCAGGAGGATATATCGGA	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
....................................................................................................................................................................................................................................AACGAGTCGGAGCAGGAGGA.........	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0
.AGCAT------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GATTTGTCTTTGAACA.....................................	21	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
CAGCAT------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GATTTGTCTTTG.........................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................................................................GTCGGAGCAGGAGGATATAT....	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droGri2

-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGATAAGTCGGG	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

droWil2

CCAGATC-ACCCATTTTCCGTATG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
No Reads

-24.9	-24.9	-24.3	-24.3

dmo_787

scaffold_6473:14442316-14442466 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads