dmo_75

AGTCTTATTCCCTCTAGATACCCTACAAACTGTATATATGTATTACTCCAATAGTCGTATACAATTATGAGTCTGAATGTTTCAATCGAAGACAATAAGTGTTTACGAATATCAGAGTATTATGATATAAGCACATACGGGAAAAGGATTATAGAGTGGCGACCC *********************************(((((((.(((((..(((.(((((.(((.((((..((((...............)))).)))).))).))))).)))..))))).))).)))).**********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V110 male body	M046 female body	V056 head	V049 head	M060 embryo	V041 embryo
..................................................ATAGTCGTATACAATTATGAGT.............................................................................................	22	0	1	104.00	104	93	7	2	0	2	0
..................................................ATAGTCGTATACAATTATGAGTC............................................................................................	23	0	1	99.00	99	95	2	2	0	0	0
............................................................................................CAATAAGTGTTTACGAATATCA...................................................	22	0	1	27.00	27	20	7	0	0	0	0
............................................................................................CAATAAGTGTTTACGAATATC....................................................	21	0	1	17.00	17	14	1	1	1	0	0
..................................................ATAGTCGTATACAATTATGAG..............................................................................................	21	0	1	16.00	16	15	0	1	0	0	0
....................................................AGTCGTATACAATTATGAGTCT...........................................................................................	22	0	1	5.00	5	3	1	1	0	0	0
..................................................ATAGTCGTATACAATTATGAGTCT...........................................................................................	24	0	1	5.00	5	4	0	0	1	0	0
...........................................................................................ACAATAAGTGTTTACGAATATCA...................................................	23	0	1	4.00	4	3	1	0	0	0	0
..................................................ATAGTCGTATACAATTATGAGTCC...........................................................................................	24	1	1	2.00	2	2	0	0	0	0	0
...........................................................................................ACAATAAGTGTTTACGAATAT.....................................................	21	0	1	2.00	2	0	2	0	0	0	0
..................................................ATAGTCGTATACAATTATGAGC.............................................................................................	22	1	1	2.00	2	2	0	0	0	0	0
..................................................ATAGTCGTATACAATTATGAGTCTC..........................................................................................	25	1	1	1.00	1	1	0	0	0	0	0
..................................................ATAGTCGTATACAATTATGAGTT............................................................................................	23	1	1	1.00	1	0	0	1	0	0	0
..................................................ATAGTCGTATAGAATTATGAGT.............................................................................................	22	1	1	1.00	1	1	0	0	0	0	0
..................................................ATAGTCTTATACAATTATGAGT.............................................................................................	22	1	1	1.00	1	1	0	0	0	0	0
..................................................ATAGTCATATACAATTATGAGT.............................................................................................	22	1	1	1.00	1	1	0	0	0	0	0
....................................................ATTCGTATGCAATTACGAGTCT...........................................................................................	22	3	1	1.00	1	0	0	1	0	0	0
............................................................................................CAATAAGTGTTTACGAATATT....................................................	21	1	1	1.00	1	1	0	0	0	0	0
...........................................................................................ACAATAAGTGTTTACGAATATC....................................................	22	0	1	1.00	1	1	0	0	0	0	0
................................................................................................AAGTGTTTACGAATATCA...................................................	18	0	1	1.00	1	0	1	0	0	0	0
...........................................................................................ACAATAAGTGTTTACGAATATCT...................................................	23	1	1	1.00	1	1	0	0	0	0	0
............................................................................................CAATAAGTGTTTACGAATATCC...................................................	22	1	1	1.00	1	1	0	0	0	0	0
...................................................TAGTCGTATACAATTATGAGTCC...........................................................................................	23	1	1	1.00	1	0	0	0	0	1	0
..................................................ATAGTCGTATCCAATTATTAGT.............................................................................................	22	2	1	1.00	1	0	0	0	1	0	0
............................................................................................CAATAAGTGTTTACGGATATCA...................................................	22	1	1	1.00	1	1	0	0	0	0	0
..................................................ATAGTCGTATCCAATTCTGAGTC............................................................................................	23	2	1	1.00	1	0	0	0	1	0	0
............................................................................................CAATAAGTGTTTACGAATAT.....................................................	20	0	1	1.00	1	1	0	0	0	0	0
............................................................................................CAATAAGTGTTTGCGAATATC....................................................	21	1	1	1.00	1	1	0	0	0	0	0
...................................................TAGTCGTATACAATTATGAGT.............................................................................................	21	0	1	1.00	1	0	1	0	0	0	0
............................................................................................CAATAAGTGTTTACGAATATCAAAAA...............................................	26	3	1	1.00	1	0	1	0	0	0	0
....................................................................................................................................ACATTCGGGATAAGGATT...............	18	2	5	0.20	1	0	0	0	0	1	0
....................................................................................................................................AACTACGGGAATAGGATT...............	18	3	20	0.05	1	0	0	0	0	0	1
........................................................................................................................................TCGGGAAAAGGATTGTGGA..........	19	3	20	0.05	1	0	1	0	0	0	0
..........................................................................GAATGTTTTAATCGACCACA.......................................................................	20	3	20	0.05	1	0	1	0	0	0	0

TCAGAATAAGGGAGATCTATGGGATGTTTGACATATATACATAATGAGGTTATCAGCATATGTTAATACTCAGACTTACAAAGTTAGCTTCTGTTATTCACAAATGCTTATAGTCTCATAATACTATATTCGTGTATGCCCTTTTCCTAATATCTCACCGCTGGG **********************************(((((((.(((((..(((.(((((.(((.((((..((((...............)))).)))).))).))))).)))..))))).))).)))).*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V110 male body	V041 embryo	V056 head
......TAAGGTAGACCTAGGGGATGT..........................................................................................................................................	21	3	2	1.00	2	1	1	0
.....................................TACGTAATGAGGGCATCAG.............................................................................................................	19	3	8	0.13	1	0	0	1
.....................................TAGATAATGAGGGCATCAG.............................................................................................................	19	3	20	0.05	1	1	0	0

Species	Coordinate	ID	Alignment
droMoj3	scaffold_6328:2908891-2909055 -	dmo_75	AGTCTTATTCCCTCTAGATACCCTACAAACTGTATATATGTATTACTCCAATAGTCGTATACAATTATGAGTCTGAATGTTTCAATCGAAGACAATAAGTGTTTACGAATATCAGAGTATTATGATATAAGCACATACGGGAAAAGGATTATAGAGTGGCGACCC
droEre2	scaffold_4690:9682463-9682522 +		GGGCATGT-CCTCCAAGCGAGCAAAGAAGTAGTATTTGTG-----TTCCAAAAGTCAAATATAACT---------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droMoj3

AGTCTTATTCCCTCTAGATACCCTACAAACTGTATATATGTATTACTCCAATAGTCGTATACAATTATGAGTCTGAATGTTTCAATCGAAGACAATAAGTGTTTACGAATATCAGAGTATTATGATATAAGCACATACGGGAAAAGGATTATAGAGTGGCGACCC	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
..................................................ATAGTCGTATACAATTATGAGT.............................................................................................	22	1	104.00	104	7	0	0	2	2	93
..................................................ATAGTCGTATACAATTATGAGTC............................................................................................	23	1	99.00	99	2	0	0	2	0	95
............................................................................................CAATAAGTGTTTACGAATATCA...................................................	22	1	27.00	27	7	0	0	0	0	20
............................................................................................CAATAAGTGTTTACGAATATC....................................................	21	1	17.00	17	1	0	1	1	0	14
..................................................ATAGTCGTATACAATTATGAG..............................................................................................	21	1	16.00	16	0	0	0	1	0	15
....................................................AGTCGTATACAATTATGAGTCT...........................................................................................	22	1	5.00	5	1	0	0	1	0	3
..................................................ATAGTCGTATACAATTATGAGTCT...........................................................................................	24	1	5.00	5	0	0	1	0	0	4
...........................................................................................ACAATAAGTGTTTACGAATATCA...................................................	23	1	4.00	4	1	0	0	0	0	3
..................................................ATAGTCGTATACAATTATGAGTCC...........................................................................................	24	1	2.00	2	0	0	0	0	0	2
...........................................................................................ACAATAAGTGTTTACGAATAT.....................................................	21	1	2.00	2	2	0	0	0	0	0
..................................................ATAGTCGTATACAATTATGAGC.............................................................................................	22	1	2.00	2	0	0	0	0	0	2
..................................................ATAGTCGTATACAATTATGAGTCTC..........................................................................................	25	1	1.00	1	0	0	0	0	0	1
..................................................ATAGTCGTATACAATTATGAGTT............................................................................................	23	1	1.00	1	0	0	0	1	0	0
............................................................................................CAATAAGTGTTTACGAATATT....................................................	21	1	1.00	1	0	0	0	0	0	1
...........................................................................................ACAATAAGTGTTTACGAATATC....................................................	22	1	1.00	1	0	0	0	0	0	1
................................................................................................AAGTGTTTACGAATATCA...................................................	18	1	1.00	1	1	0	0	0	0	0
...........................................................................................ACAATAAGTGTTTACGAATATCT...................................................	23	1	1.00	1	0	0	0	0	0	1
............................................................................................CAATAAGTGTTTACGAATATCC...................................................	22	1	1.00	1	0	0	0	0	0	1
...................................................TAGTCGTATACAATTATGAGTCC...........................................................................................	23	1	1.00	1	0	0	0	0	1	0
............................................................................................CAATAAGTGTTTACGAATAT.....................................................	20	1	1.00	1	0	0	0	0	0	1
...................................................TAGTCGTATACAATTATGAGT.............................................................................................	21	1	1.00	1	1	0	0	0	0	0

droEre2

GGGCATGT-CCTCCAAGCGAGCAAAGAAGTAGTATTTGTG-----TTCCAAAAGTCAAATATAACT---------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
..............AAGCGAGCAAAGAAGTAGTATT.................................................................................................................................	22	1	3284.00	3284	5	0	7	0	2953	319	0
..............AAGCGAGCAAAGAAGTAGTATTT................................................................................................................................	23	1	1614.00	1614	2	0	11	0	1458	143	0
..............AAGCGAGCAAAGAAGTAGTAT..................................................................................................................................	21	1	85.00	85	0	0	0	0	84	1	0
..............AAGCGAGCAAAGAAGTAGTATTTT...............................................................................................................................	24	1	43.00	43	0	0	0	0	41	2	0
..............AAGCGAGCAAAGAAGTAGTA...................................................................................................................................	20	1	10.00	10	0	0	0	0	10	0	0
..............AAGCGAGCAAAGAAGTAGTATTTA...............................................................................................................................	24	1	5.00	5	0	0	0	0	5	0	0
...............AGCGAGCAAAGAAGTAGTATT.................................................................................................................................	21	1	5.00	5	2	0	0	0	3	0	0
..............AAGCGAGCAAAGAAGTAGT....................................................................................................................................	19	1	4.00	4	0	0	0	0	4	0	0
..............AAGCGAGCAAAGAAGTAGTATTTG...............................................................................................................................	24	1	3.00	3	0	0	0	0	3	0	0
.................CGAGCAAAGAAGTAGTATT.................................................................................................................................	19	1	2.00	2	0	0	0	0	1	1	0
...............AGCGAGCAAAGAAGTAGTATTT................................................................................................................................	22	1	2.00	2	0	0	0	0	2	0	0
.............CAAGCGAGCAAAGAAGTAGTATTTT...............................................................................................................................	25	1	1.00	1	0	0	0	0	1	0	0
..............AAGCGAGCAAAGAAGTAGTATTG................................................................................................................................	23	1	1.00	1	0	0	0	0	1	0	0
..............AAGCGAGCAAAGAAGTAGTATTC................................................................................................................................	23	1	1.00	1	0	0	0	0	0	1	0
..................GAGCAAAGAAGTAGTATT.................................................................................................................................	18	1	1.00	1	0	0	0	0	1	0	0
................GCGAGCAAAGAAGTAGTATT.................................................................................................................................	20	1	1.00	1	0	0	0	0	1	0	0
..............AAGCGAGCAAAGAAGTAGTATTA................................................................................................................................	23	1	1.00	1	0	0	0	0	0	1	0
.............CAAGCGAGCAAAGAAGTAGTATTT................................................................................................................................	24	1	1.00	1	0	0	0	0	0	1	0
...............AGCGAGCAAAGAAGTAGTAT..................................................................................................................................	20	1	1.00	1	0	0	0	0	1	0	0
................GCGAGCAAAGAAGTAGTATTT................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0	0
..............AAGCGAGCAAAGAAGTAGTATTAA...............................................................................................................................	24	1	1.00	1	0	0	0	0	1	0	0
..............AAGCGAGCAAAGAAGTAGTATA.................................................................................................................................	22	1	1.00	1	0	0	0	0	1	0	0
.............CAAGCGAGCAAAGAAGTAGTATT.................................................................................................................................	23	1	1.00	1	0	0	0	0	1	0	0
..............AAGCGAGCAAAGAAGTAGTATC.................................................................................................................................	22	1	1.00	1	0	0	0	0	1	0	0

dmo_75

scaffold_6328:2908941-2909005 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-21.1	-20.7	-20.6