dmo_465

--------------------##############################--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- TTTCCATCACCTGTGGCAAGATGTCCTTTGTGCGTCTAATTGGTGCTGAGGTAAGAAATTCTATTTGCATATGCTACCAACGGCTATCGATAACAATTGCGCAGTGTTAGGAAGCCTAAGAGGGGAGGGGGCGTTGAACACTTGCAAGTGCACTGGCAAGTGGGCGTGTATGTGTGTGTGTTTGTGCAGTGCCTTGCACTTGCATGCAAATTAAAGCACGCGTGCTTGCCTGTGTATGTGTGTGTGCTCAT **************************************************************.(((((.(((((......((............(((((.((((((.....((((...........))))....)))))))))))..))..))))).)))..))***********************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M046 female body	V056 head	V110 male body	M060 embryo	V041 embryo	V049 head
.................................................................................................................................................AAGTGCACTGGCAAGTGGGCG.....................................................................................	21	0	1	3.00	3	3	0	0	0	0	0
....................................................................................................................................................TGCACTGGCAAGTGGGCGTG...................................................................................	20	0	1	2.00	2	0	0	2	0	0	0
.................................................................................................................................................AAGTGCACTGGCAAGTGGGC......................................................................................	20	0	1	2.00	2	1	0	0	0	1	0
....................................................................................................................................................TGCACTGGCAAGTGGGCGTGT..................................................................................	21	0	1	2.00	2	1	1	0	0	0	0
.................................................................................................................................................AAGTGCACTGGCAAGTGG........................................................................................	18	0	1	2.00	2	1	0	0	1	0	0
............................................................................................................................................................................................................................CGGGCTTGCCTGTGTATGTGT..........	21	1	1	2.00	2	0	2	0	0	0	0
...................GATGTCCTTTGTGCGTCTAATTGGTGC.............................................................................................................................................................................................................	27	0	1	1.00	1	1	0	0	0	0	0
.................AAGATGTCCTTTGTGCGT........................................................................................................................................................................................................................	18	0	1	1.00	1	1	0	0	0	0	0
.................................................................................................................................................AAGTGCACTGGCAAGTGGGCGTG...................................................................................	23	0	1	1.00	1	1	0	0	0	0	0
..................................TCTAATTGGTGCTGAGGCACGT...................................................................................................................................................................................................	22	3	1	1.00	1	1	0	0	0	0	0
.......CACCTGTGGCAAGATGTC..................................................................................................................................................................................................................................	18	0	1	1.00	1	0	0	0	0	1	0
..................................................................................................................................................AGTGCACTGGCAAGTGGGCG.....................................................................................	20	0	1	1.00	1	1	0	0	0	0	0
................................................................TTGCATATGCTACCAACGGCTAT....................................................................................................................................................................	23	0	1	1.00	1	0	0	0	1	0	0
......TCACCTGTGGCAAGATGTCCTTTGTGC..........................................................................................................................................................................................................................	27	0	1	1.00	1	0	0	1	0	0	0
............GTGGCAAGATGTCCTTTGTGCGT........................................................................................................................................................................................................................	23	0	1	1.00	1	0	0	1	0	0	0
...............GCAAGATGTCCTTTGTGCGT........................................................................................................................................................................................................................	20	0	1	1.00	1	1	0	0	0	0	0
.................AAGATGTCCTTTGTGCGTCTAATTGGT...............................................................................................................................................................................................................	27	0	1	1.00	1	1	0	0	0	0	0
.................................................................................................................................................AAGTGCACTGGCAAGTGGG.......................................................................................	19	0	1	1.00	1	1	0	0	0	0	0
.................................................................................................................................................AAGTGCACTGGCAAGTGGGCGC....................................................................................	22	1	1	1.00	1	0	1	0	0	0	0
....................................................................................................................................................TTAACTGGCAAGTGGGCGTGTA.................................................................................	22	2	1	1.00	1	0	0	0	1	0	0
............................................................................................................................................................................................................................CGGGCTTGCCTGTGTATGTTT..........	21	2	2	0.50	1	0	0	0	0	0	1
...................................................................................................................................................................................................................TAAGGCACGCGTGAATGCC.....................	19	3	17	0.41	7	0	2	0	4	0	1
..........................................ATGCTGAGGTAAGAAAGT...............................................................................................................................................................................................	18	2	7	0.14	1	0	0	1	0	0	0
..............................................................................................AATCGGGCAGTGTTAGGA...........................................................................................................................................	18	2	8	0.13	1	0	1	0	0	0	0
..........................TTTGTGCGTCTGATTGCGGCT............................................................................................................................................................................................................	21	3	12	0.08	1	0	1	0	0	0	0
.................................................................................................................................................AAGGGCAATGGCGAGTGGGCG.....................................................................................	21	3	14	0.07	1	0	0	0	0	0	1

AAAGGTAGTGGACACCGTTCTACAGGAAACACGCAGATTAACCACGACTCCATTCTTTAAGATAAACGTATACGATGGTTGCCGATAGCTATTGTTAACGCGTCACAATCCTTCGGATTCTCCCCTCCCCCGCAACTTGTGAACGTTCACGTGACCGTTCACCCGCACATACACACACACAAACACGTCACGGAACGTGAACGTACGTTTAATTTCGTGCGCACGAACGGACACATACACACACACGAGTA ***********************************************************************************.(((((.(((((......((............(((((.((((((.....((((...........))))....)))))))))))..))..))))).)))..))**************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M046 female body	V049 head
....................................................................................................................................................................................................GTGAACGTACGTTTAGTTTCGT.................................	22	1	1	1.00	1	1	0
..........................................................................................................................................................................ACACACACACAAACGCGTCAC............................................................	21	1	7	0.14	1	0	1

Species	Coordinate	ID	Alignment
droMoj3	scaffold_6328:646382-646632 -	dmo_465	TTTCCATCACCTGTGGCAAGATGTCCTTTGTGCGTCTAATTGGTGCTGAGGTAAGAA-AT-TC-------------------------TATTTGCATATGCTACCAACGGC-----------------------TATCGATAACA--ATT----------GCGCAGTGT----------------------TAGGAAGCCTAAGAGGGGAGGGGGCGTTGA------ACACTT--------------GCAAGTGCACTGGCAAGTG-----GGCGTGTATG------TGTGTGTGTTTGTGCAGTGCCTTGCACTTGCATGCAAATTA--A------------AGCACGCGTGCTT--------GCCTGTGTAT----GTGTGTGTGCTCAT--------------------------------------------------------------
droVir3	scaffold_13042:3014053-3014302 +	dvi_14523	CTTCAACAACCTGT-GGAAGATGTCCTTTGTGCGTCTAATTGGTGCTGAGGTAAGAA-AT-T----------------------------------------------------CAATAAGACATCTAAACACATATCGATAACA--ATTGTAATATGTGGCGCAGTGT----------------------TGGAAAGTTTATGAGGGG------GGCGT---------------------------GATGTTGCATTGCCGTGT---------------G----TGTGTGTGTGTGTGTG----------TGTGTGCATGCAAATTACAATGTTTCAATGCACGTGCA------TGCATGTGTG----TGTGTGTGTGTGTGTGTGCTGT-----------------------------------------------------GAGGGGTTGT
droGri2	scaffold_14853:1291492-1291736 +		TTCCTG--ACCTGT-TCAAGATGTCCTTTGTTCGTCTAATTGGTGCCGAGGTAAGTT-AATTG---------------------------------------------------TAATT------ACTCAAT--TGTCGACAACATAGTTGCATTGAAGTGTGCCATGC----------------------TGGAAAGCTTACA--------ATGTACAT---------------------------TAAAGTGCATTGCCATATG-TAAATGCGCTTGTGTGTGTGTATGTGTGTTTACA----------TATGTGCATGCAATTTGCAACTGCTTAATGCATATTCC------GATTTATTTG----CGTGTGTGTGTA------TGCAT--------------------------------------------------------------
droWil2	scf2_1100000004590:1347025-1347084 +		CCTTTC----GC----------------------------------TTAA------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCAAATTGCAA------------TGTACGTGTG--------TGTGTGTGTGTGT----GTGTGTGTGCTGTT--------------------------------------------------------------
dp5	XL_group1e:4397739-4397794 -		TCTCTGTCTCTCCAACCAGGATGGCCTTCCTGCGTGCAATCGGTGCTGAGGTAAGA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droPer2	scaffold_45:160235-160303 +		GTATGT----AT------GTATGTTGCTTGTGTGTTGT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GTGTACGAATTA---------------TGCATG------------TTTG----TGTATGTGTGTGTG----TGTAT--------------------------------------------------------------
droAna3	scaffold_13117:5118602-5118657 -		CATC------------CACGATGTCCCTTGTGCGTTTAATTGGAGCTGAGGTGAGTTTAATTC---------------------------------------------------CAATT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droBip1	scf7180000396734:99186-99309 +		ATCCAC----ATAT-CCACGATGTCCCTTGTGCGTTTAATTGGAGCTGAGGTGAGTT-AA-----------------------------------------------TGACTTCCAATTAGAATTCCCCCCC-----------------------------------------------------------TGGGAAG----AAAGGGGAGAGAGTATAG------------T--------------GAGAGTGTAATGGCACATG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droKik1	scf7180000302592:869637-869667 -		GCAGGG------------AGGG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GTGTGTG----------------------------------------------------------------------------------------CGTGT----GTGTTTGTG-------------------------------------------------------------------
droFic1	scf7180000453927:858431-858729 -		TCCC-----------CCAAGATGTCCCTAGTGCGATTGATCGGCGCTGAGGTAAGTT-ACTCGGCTGTTTTCCAATATTTACAAAATCATATTGCATAC--------CGGT-----------------------GATCTAAAACA--GGT----------AATCTGTGTGGATGCATCCCACTTTTCACCTAAGAGATCTTACA--------ATGTGTGGAATATAAATATTTCCCTCTTATTTTACTCAATTAAACGGTCAGCTTTTAAATGCCTGTGTG----------------------------------------------------------------------------------------TGTGT----GGGTGGGTGCCCTTGAACTTGCAAAACAACACACGTGCAGCATTTATGTAAAACTGCACTTTTTATCTGCCCCCTT
droEle1	scf7180000491272:675118-675171 -		TATCC----------CCAAGATGTCCCTAGTGCGTTTGATCGGTGCTGAGGTGAGTT-TT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTTG--------------------------------------------------------------
droRho1	scf7180000776532:201798-201840 -		TCCC------------CAAGATGTCCCTAGTGCGTTTGATCGGCGCTGAGGTGAG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droBia1	scf7180000301760:1418989-1419036 -		CCGTAT------CC-CCAAGATGTCCCTGGTCCGTTTGATCGGTGCTGAGGTGAG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droTak1	scf7180000415179:420987-421033 -		AATAT-------CC-CCAAGATGTCCCTAGTGCGTTTGATCGGTGCTGAGGTGAG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEug1	scf7180000409277:757912-757954 -		TCCT------------CAAGATGTCCCTAGTTCGTTTGATCGGCGCTGAGGTAAG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droSim2	x:11977518-11977604 +		TGAGTGTGTGTGTGTGTGTGTTTGTGAAGCGGCATGCAATT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCA--GCTTGAATTTATGCGCACTTGCGT--------GTGTGTGTGT----GTGTGTGTG-------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droMoj3

TTTCCATCACCTGTGGCAAGATGTCCTTTGTGCGTCTAATTGGTGCTGAGGTAAGAA-AT-TC-------------------------TATTTGCATATGCTACCAACGGC-----------------------TATCGATAACA--ATT----------GCGCAGTGT----------------------TAGGAAGCCTAAGAGGGGAGGGGGCGTTGA------ACACTT--------------GCAAGTGCACTGGCAAGTG-----GGCGTGTATG------TGTGTGTGTTTGTGCAGTGCCTTGCACTTGCATGCAAATTA--A------------AGCACGCGTGCTT--------GCCTGTGTAT----GTGTGTGTGCTCAT--------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
.........................................................................................................................................................................................................................................................AAGTGCACTGGCAAGTG-----GGCG...................................................................................................................................................................................	21	1	3.00	3	3	0	0	0	0	0
............................................................................................................................................................................................................................................................TGCACTGGCAAGTG-----GGCGTG.................................................................................................................................................................................	20	1	2.00	2	0	0	0	0	0	2
.........................................................................................................................................................................................................................................................AAGTGCACTGGCAAGTG-----GGC....................................................................................................................................................................................	20	1	2.00	2	1	1	0	0	0	0
............................................................................................................................................................................................................................................................TGCACTGGCAAGTG-----GGCGTGT................................................................................................................................................................................	21	1	2.00	2	1	0	0	1	0	0
.........................................................................................................................................................................................................................................................AAGTGCACTGGCAAGTG-----G......................................................................................................................................................................................	18	1	2.00	2	1	0	0	0	1	0
...................GATGTCCTTTGTGCGTCTAATTGGTGC........................................................................................................................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	1	0	0	0	0	0
.................AAGATGTCCTTTGTGCGT...................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	1	0	0	0	0	0
.........................................................................................................................................................................................................................................................AAGTGCACTGGCAAGTG-----GGCGTG.................................................................................................................................................................................	23	1	1.00	1	1	0	0	0	0	0
.......CACCTGTGGCAAGATGTC.............................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	1	0	0	0	0
..........................................................................................................................................................................................................................................................AGTGCACTGGCAAGTG-----GGCG...................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
...........................................................................................TTGCATATGCTACCAACGGC-----------------------TAT.............................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	1	0
......TCACCTGTGGCAAGATGTCCTTTGTGC.....................................................................................................................................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	1
............GTGGCAAGATGTCCTTTGTGCGT...................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	1
...............GCAAGATGTCCTTTGTGCGT...................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
.................AAGATGTCCTTTGTGCGTCTAATTGGT..........................................................................................................................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	1	0	0	0	0	0
.........................................................................................................................................................................................................................................................AAGTGCACTGGCAAGTG-----GG.....................................................................................................................................................................................	19	1	1.00	1	1	0	0	0	0	0
.........................................................................................................................................................................................................................................................AAGTGCACTGGCAAGTG-----GGCGC..................................................................................................................................................................................	22	1	1.00	1	0	0	0	1	0	0

droVir3

CTTCAACAACCTGT-GGAAGATGTCCTTTGTGCGTCTAATTGGTGCTGAGGTAAGAA-AT-T----------------------------------------------------CAATAAGACATCTAAACACATATCGATAACA--ATTGTAATATGTGGCGCAGTGT----------------------TGGAAAGTTTATGAGGGG------GGCGT---------------------------GATGTTGCATTGCCGTGT---------------G----TGTGTGTGTGTGTGTG----------TGTGTGCATGCAAATTACAATGTTTCAATGCACGTGCA------TGCATGTGTG----TGTGTGTGTGTGTGTGTGCTGT-----------------------------------------------------GAGGGGTTGT	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
.......AACCTGT-GGAAGATGTCCTTTGTGC.....................................................................................................................................................................................................................................................................................................................................................................................................................................	25	1	7.00	7	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	3	0
.......AACCTGT-GGAAGATGTCCTTTGTGCGT...................................................................................................................................................................................................................................................................................................................................................................................................................................	27	1	3.00	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
....AACAACCTGT-GGAAGATGTCCTTTGTGC.....................................................................................................................................................................................................................................................................................................................................................................................................................................	28	1	3.00	3	0	0	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0
...........TGT-GGAAGATGTCCTTTGTG......................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	2.00	2	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........TGT-GGAAGATGTCCTTTGTGC.....................................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0
.....ACAACCTGT-GGAAGATGTCCTTTGTG......................................................................................................................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................AGATGTCCTTTGTGCGTC..................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
............................................................................................................................................................................................................................................................................................................................................................................................GTGTGTGCTGT-----------------------------------------------------GAGGGGTT..	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
......CAACCTGT-GGAAGATGTCC............................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0
...CAACAACCTGT-GGAAGATGTCCTTTGTGC.....................................................................................................................................................................................................................................................................................................................................................................................................................................	29	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................TGCGTCTAATTGGTGCTGAGG...................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............GT-GGAAGATGTCCTTTGTGCGTCT.................................................................................................................................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
................GAAGATGTCCTTTGTGCGTCTA................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................TGTCCTTTGTGCGTCTAATTGGTGC........................................................................................................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...................GATGTCCTTTGTGCGTCTAATTGGTG.........................................................................................................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
...........................TTGTGCGTCTAATTGGTGCTG......................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....ACAACCTGT-GGAAGATGTCCTTTGTGC.....................................................................................................................................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............GT-GGAAGATGTCCTTTGTGC.....................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
................GAAGATGTCCTTTGTGCGTCTAATTGGT..........................................................................................................................................................................................................................................................................................................................................................................................................................	28	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
....AACAACCTGT-GGAAGATGTCCT...........................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......CAACCTGT-GGAAGATGTCCTTTGTGC.....................................................................................................................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....ACAACCTGT-GGAAGATGTCC............................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
CTTCAACAACCTGT-GGAAGATGTCC............................................................................................................................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
........ACCTGT-GGAAGATGTCCTTTGC.......................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............GT-GGAAGATGTCCTTTGTGCGTC..................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........CCTGT-GGAAGATGTCCTTTGTGC.....................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0
..............................................................................................................................................................................................................................................................CATTGCCGTGT---------------G----TGTGTG...................................................................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................................................................................................................................................................................CGTGCA------TGCATGTGTG----TG...................................................................................	18	4	0.25	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0
.........................................................................................................................................................................................................................................................................................................................................................................TGTG----TGTGTGTGTGTGTGTG.....................................................................	20	20	0.10	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
........................................................................................................................................................................................................................................................................................G----TGTGTGTGTGTGTGTG----------TGTGTGC........................................................................................................................................	24	20	0.05	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0

droGri2

TTCCTG--ACCTGT-TCAAGATGTCCTTTGTTCGTCTAATTGGTGCCGAGGTAAGTT-AATTG---------------------------------------------------TAATT------ACTCAAT--TGTCGACAACATAGTTGCATTGAAGTGTGCCATGC----------------------TGGAAAGCTTACA--------ATGTACAT---------------------------TAAAGTGCATTGCCATATG-TAAATGCGCTTGTGTGTGTGTATGTGTGTTTACA----------TATGTGCATGCAATTTGCAACTGCTTAATGCATATTCC------GATTTATTTG----CGTGTGTGTGTA------TGCAT--------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
................................................................................................................................................................................................................................................................................................ATGTGTGTTTACA----------TATGTGCAT......................................................................................................................................	22	1	1.00	1	1	0

droWil2

CCTTTC----GC----------------------------------TTAA------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCAAATTGCAA------------TGTACGTGTG--------TGTGTGTGTGTGT----GTGTGTGTGCTGTT--------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
.........................................................................................................................................................................................................................................................................................................................................................TACGTGTG--------TGTGTGTGTGTGT----G...........................................................................	22	20	0.10	2	0	0	0	0	2
..........................................................................................................................................................................................................................................................................................................................................................ACGTGTG--------TGTGTGTGTGT..................................................................................	18	20	0.05	1	0	0	0	0	1
...............................................................................................................................................................................................................................................................................................................................................................................TGTGTGT----GTGTGTGTGCT.................................................................	18	20	0.05	1	0	0	0	1	0
.........................................................................................................................................................................................................................................................................................................................................................TACGTGTG--------TGTGTGTGTG...................................................................................	18	20	0.05	1	0	0	0	0	1

dp5

TCTCTGTCTCTCCAACCAGGATGGCCTTCCTGCGTGCAATCGGTGCTGAGGTAAGA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
...CTGTCTCTCCAACCAGGATGGC.............................................................................................................................................................................................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	1	0	0	0	0	0
..................GGATGGCCTTCCTGCGTGCAATCGG...........................................................................................................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.CTCTGTCTCTCCAACCAGG..................................................................................................................................................................................................................................................................................................................................................................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...........................TCCTGCGTGCAATCGGTGCTGAGGCC.................................................................................................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...............................GCGTGCAATCGGTGCTGA.....................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	1	0	0	0	0	0	0	0	0
................CAGGATGGCCTTCCTGCGTGCAATC.............................................................................................................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	1	0	0	0	0	0	0
....................ATGGCCTTCCTGCGTGCA................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.................................GTGCAATCGGTGCTGAGGCC.................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	1	0	0	0	0	0
.....................TGGCCTTCCTGCGTGCAATCG............................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0

droPer2

GTATGT----AT------GTATGTTGCTTGTGTGTTGT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GTGTACGAATTA---------------TGCATG------------TTTG----TGTATGTGTGTGTG----TGTAT--------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
No Reads

droAna3

CATC------------CACGATGTCCCTTGTGCGTTTAATTGGAGCTGAGGTGAGTTTAATTC---------------------------------------------------CAATT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
...C------------CACGATGTCCCTTGTGCGTTTAATTGGA..........................................................................................................................................................................................................................................................................................................................................................................................................................	29	1	1.00	1	0	1	0	0	0	0
......................GTCCCTTGTGCGTTTAATTGGAGCT.......................................................................................................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	1	0	0	0	0
..................CGATGTCCCTTGTGCGTTTAATTGGAG.........................................................................................................................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	1	0
.............................GTGCGTTTAATTGGAGCTGA.....................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0
..........................CTTGTGCGTTTAATTGGAGCTGAGGCT.................................................................................................................................................................................................................................................................................................................................................................................................................	27	1	1.00	1	0	1	0	0	0	0
..................CGATGTCCCTTGTGCGTT..................................................................................................................................................................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	1	0	0	0	0
CATC------------CACGATGTCCCTTGTGCGTTTG................................................................................................................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	1	0	0	0	0	0
...........................TTGTGCGTTTAATTGGAGCTGAGGCC.................................................................................................................................................................................................................................................................................................................................................................................................................	26	1	1.00	1	0	1	0	0	0	0
.............................GTGCGTTTAATTGGAGCTGAGGCT.................................................................................................................................................................................................................................................................................................................................................................................................................	24	1	1.00	1	0	1	0	0	0	0
CATC------------CACGATGTCCCTTGTGC.....................................................................................................................................................................................................................................................................................................................................................................................................................................	21	1	1.00	1	0	1	0	0	0	0
................CACGATGTCCCTTGTGCGTT..................................................................................................................................................................................................................................................................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
....................ATGTCCCTTGTGCGTTTAATTGGAG.........................................................................................................................................................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	1	0	0	0	0
CATC------------CACGATGTCCCTTGTGCGC...................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	1	0	0	0	0

droBip1

ATCCAC----ATAT-CCACGATGTCCCTTGTGCGTTTAATTGGAGCTGAGGTGAGTT-AA-----------------------------------------------TGACTTCCAATTAGAATTCCCCCCC-----------------------------------------------------------TGGGAAG----AAAGGGGAGAGAGTATAG------------T--------------GAGAGTGTAATGGCACATG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droKik1

GCAGGG------------AGGG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GTGTGTG----------------------------------------------------------------------------------------CGTGT----GTGTTTGTG-------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droFic1

TCCC-----------CCAAGATGTCCCTAGTGCGATTGATCGGCGCTGAGGTAAGTT-ACTCGGCTGTTTTCCAATATTTACAAAATCATATTGCATAC--------CGGT-----------------------GATCTAAAACA--GGT----------AATCTGTGTGGATGCATCCCACTTTTCACCTAAGAGATCTTACA--------ATGTGTGGAATATAAATATTTCCCTCTTATTTTACTCAATTAAACGGTCAGCTTTTAAATGCCTGTGTG----------------------------------------------------------------------------------------TGTGT----GGGTGGGTGCCCTTGAACTTGCAAAACAACACACGTGCAGCATTTATGTAAAACTGCACTTTTTATCTGCCCCCTT	Size	Hit Count	Total Norm	Total
No Reads

droEle1

TATCC----------CCAAGATGTCCCTAGTGCGTTTGATCGGTGCTGAGGTGAGTT-TT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTTG--------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droRho1

TCCC------------CAAGATGTCCCTAGTGCGTTTGATCGGCGCTGAGGTGAG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droBia1

CCGTAT------CC-CCAAGATGTCCCTGGTCCGTTTGATCGGTGCTGAGGTGAG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droTak1

AATAT-------CC-CCAAGATGTCCCTAGTGCGTTTGATCGGTGCTGAGGTGAG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEug1

TCCT------------CAAGATGTCCCTAGTTCGTTTGATCGGCGCTGAGGTAAG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droSim2

TGAGTGTGTGTGTGTGTGTGTTTGTGAAGCGGCATGCAATT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCA--GCTTGAATTTATGCGCACTTGCGT--------GTGTGTGTGT----GTGTGTGTG-------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M023 Head	M024 Male-body	M025 Embryo	M053 Female-body	O001 Testis	O002 Head	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary	V044 Embryo	SRR902008 Ovary	SRR902009 Testis	SRR1275487 Male larvae	SRR1275485 Male prepupae	SRR1275483 Male prepupae
......TGTGTGTGTGTGTGTTTGTGA...........................................................................................................................................................................................................................................................................................................................................................................................................................................	21	6	0.17	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................................................................................................................................................GT--------GTGTGTGTGT----GTGTGTGTG-------------------------------------------------------------------	21	20	0.05	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0
...GTGTGTGTGTGTGTGTGTTTG..............................................................................................................................................................................................................................................................................................................................................................................................................................................	21	20	0.05	1	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0

-25.8	-25.2	-25.1	-25.1

dmo_465

scaffold_6328:646432-646582 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads