ID:

dmo_288

Coordinate:

scaffold_6680:16725177-16725238 -

Confidence:

candidate

Class:

Canonical miRNA

Genomic Locale:

intron

[View on UCSC Genome Browser {Cornell Mirror}]

Legend:

mature

star

mismatch in alignment

mismatch in read

Predicted structure

-20.2	-20.1	-20.0

Flybase annnotation

intron [Dmoj\GI11698-in]

No Repeatable elements found

Sense Strand Reads

AGCACTCAGCGAACTTGTTCGCATGAACCAGCTACGCAACTCTTTGAGCAACTGTTAATATTTTGCTTATCTCTGACACACATACGAAGCTGAGCATATGTATTACAGCTTTGTCAAAGTGTTATACACACAGAGTAGAGGTTGGGTTAGTGTTATAATAAA *********************************..(((.((((((..((((((.(((((..((((((.((.((.........)).)).))))))...))))))))).))..)))))).)))...**********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M060 embryo	M046 female body	V049 head	V041 embryo	V110 male body	V056 head
.........................................................................................CTGAGCATATGTATTACAGCTT...................................................	22	0	1	12.00	12	7	1	3	0	1	0
..................................................ACTGTTAATATTTTGCTTATCT..........................................................................................	22	0	1	8.00	8	8	0	0	0	0	0
.........................................................................................CTGAGCATATGTATTACAGCTTT..................................................	23	0	1	3.00	3	2	1	0	0	0	0
.........................................................................................CTGAGCATATGTATTACAGCT....................................................	21	0	1	3.00	3	2	0	0	1	0	0
..................................................ACTGTTAATATTTTGCTTATCC..........................................................................................	22	1	1	2.00	2	2	0	0	0	0	0
..................................................ACTGTTAATATTTTGCTTATC...........................................................................................	21	0	1	1.00	1	0	1	0	0	0	0
.........................................................................................CGGAGCATATGTATTACAGCTT...................................................	22	1	1	1.00	1	1	0	0	0	0	0
.........................................................................................CTGAGCATATGTCTTACAGCTT...................................................	22	1	1	1.00	1	1	0	0	0	0	0
.........................................................................................CGGAGCATATGTATTACAGCTTT..................................................	23	1	1	1.00	1	1	0	0	0	0	0
.........................................................................................CGGTGCATATGTATTACAGCTT...................................................	22	2	1	1.00	1	1	0	0	0	0	0
.............CTTGTGCGCGTGAACCAGCTT................................................................................................................................	21	3	2	0.50	1	0	0	0	0	0	1
.............CTTGTCCCCGTGAACCAGCT.................................................................................................................................	20	3	5	0.20	1	0	0	0	0	0	1
.............CTGGTCCGCTTGAACCAGCT.................................................................................................................................	20	3	6	0.17	1	0	0	0	0	0	1

Anti-sense strand reads

TCGTGAGTCGCTTGAACAAGCGTACTTGGTCGATGCGTTGAGAAACTCGTTGACAATTATAAAACGAATAGAGACTGTGTGTATGCTTCGACTCGTATACATAATGTCGAAACAGTTTCACAATATGTGTGTCTCATCTCCAACCCAATCACAATATTATTT **********************************..(((.((((((..((((((.(((((..((((((.((.((.........)).)).))))))...))))))))).))..)))))).)))...*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Type	Alignment
droMoj3	scaffold_6680:16725127-16725288 -	dmo_288	candidate	AGCACTC--AGCGAACTTGTTCGCAT---------------------GAACCAGCTACGCAACTCTTTGAGCAACTGTTAATATTTTGCTTATCT-----------------CTGACACACATACG----AAGCTGAGCATAT----GTATTACAGCTTTGTCAAAGTGTTATACACACAGAGTAGAGGTTGGGTTAGTGTTATAATAAA
droVir3	scaffold_13049:22129255-22129458 -			TACTCTCTATGCGAACTAGTTCGCATACACTTATCTGTAAATACGAAGAACCAGCTGAACAACTCTTTTAACAAGTTATATCA-TTTGGTTATCTGCATATTTATGCACACACTGACACACATACCCATATACCTATGCCTATATCTATATTGCAGCGCTGCAAAACCATTCTAC----AACGTAGATATTA-TTTAGCAATATTATGAA

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droMoj3

AGCACTC--AGCGAACTTGTTCGCAT---------------------GAACCAGCTACGCAACTCTTTGAGCAACTGTTAATATTTTGCTTATCT-----------------CTGACACACATACG----AAGCTGAGCATAT----GTATTACAGCTTTGTCAAAGTGTTATACACACAGAGTAGAGGTTGGGTTAGTGTTATAATAAA	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
.....................................................................................................................................CTGAGCATAT----GTATTACAGCTT...................................................	22	1	12.00	12	1	0	3	0	7	1
.........................................................................ACTGTTAATATTTTGCTTATCT-----------------..................................................................................................	22	1	8.00	8	0	0	0	0	8	0
.....................................................................................................................................CTGAGCATAT----GTATTACAGCTTT..................................................	23	1	3.00	3	1	0	0	0	2	0
.....................................................................................................................................CTGAGCATAT----GTATTACAGCT....................................................	21	1	3.00	3	0	1	0	0	2	0
.........................................................................ACTGTTAATATTTTGCTTATCC-----------------..................................................................................................	22	1	2.00	2	0	0	0	0	2	0
.........................................................................ACTGTTAATATTTTGCTTATC....................................................................................................................	21	1	1.00	1	1	0	0	0	0	0

droVir3

TACTCTCTATGCGAACTAGTTCGCATACACTTATCTGTAAATACGAAGAACCAGCTGAACAACTCTTTTAACAAGTTATATCA-TTTGGTTATCTGCATATTTATGCACACACTGACACACATACCCATATACCTATGCCTATATCTATATTGCAGCGCTGCAAAACCATTCTAC----AACGTAGATATTA-TTTAGCAATATTATGAA	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells	SRR2096013 Ovary_Strain_9_oxidized	SRR2096014 Ovary_Strain_160_oxidized	SRR2096026 embryo_0-2h from 14 day old Strain 9 mothers	SRR2096038 embryo_0-2h from 14 day old Strain 9 mothers	SRR2096050 embryo_0-2h from 14 day old Strain 160 mothers	SRR2096055 Ovary_Min_43	SRR2096056 Ovary_Min_46	SRR2096057 Ovary_Min_50	SRR2096058 Ovary_Min_98	SRR2096059 Ovary_Min_164	SRR2096060 Ovary_Min_193	SRR2096061 embryo_0-2h from 14 day old Strain 160 mothers	SRR2096072 embryo_0-2h from 15to16 day old dysgenic mothers	SRR2096079 embryo_0-2h from 15to16 day old dysgenic mothers	SRR2096080 embryo_0-2h from 15to16 day old nondysgenic mothers	SRR2096081 embryo_0-2h from 15to16 day old nondysgenic mothers
..................................................................................................................................................................AAAACCATTCTAC----AACGTAG........................	20	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................CCAGCTGAACAACTCTTTTAACAAGTTT....................................................................................................................................	28	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0
.....................................TAAATACGAAGAACCAGCTGAACAAC...................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0

Generated: 10/20/2015 at 07:02 PM