dmo_179

TACAACTAAAAGATATAGATACACCAAAAAGATACACTCTAAAGATACAGAGACGGCTGAAAGATACAGATAGAGCAAGCAGATACAGTTAAAAGATACATATTTGAATGCATCTGTATCTTTTAGCTGTAACTGTATCTTTTATATATACAGGTATCTTTTAAAAGTATCAATACCTGCTGTATC *********************************.....((((((((((..(((((((((((((((((((...(((..((((((..((........)).))))))..))))))))))))))))))))))..))))))))))........**********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M046 female body	V110 male body
................................................................................................................TCTGTATCTTTTAGCTGTAACTG...................................................	23	0	2	2.50	5	2	3
..................................................................................................................TGTATCTTTTAGCTGTAACTGT..................................................	22	0	2	2.00	4	2	2
................................................................................................................TCTGTATCTTTTAGCTGTAACTGT..................................................	24	0	2	1.00	2	1	1
..................................................AGACGGCTGAAAGATACAGAT...................................................................................................................	21	0	1	1.00	1	0	1
.......................................................................................................................CTTTTAGCTGTAACTGTATCTT.............................................	22	0	1	1.00	1	1	0
...........................................................................CAAGCAGATACAGTTAAAAGAT.........................................................................................	22	0	1	1.00	1	1	0
...................................................GACGGCTGAAAGATACAGATAGAG...............................................................................................................	24	0	1	1.00	1	0	1
...................................................GACGGCTGAAAGATACAGATAGAGCA.............................................................................................................	26	0	1	1.00	1	0	1
....ACTAAAAGATATAGATACACC.................................................................................................................................................................	21	0	1	1.00	1	1	0
........................................................................................................................TTTTAGCTGTAACTGTATCTT.............................................	21	0	1	1.00	1	1	0
..................................................AGACGGCTGAAAGATACAGA....................................................................................................................	20	0	1	1.00	1	0	1
..............................................................................................................CATCTGTATCTTTTAGCTGTAAC.....................................................	23	0	1	1.00	1	0	1
.....................................................................................................................ATCTTTTAGCTGTAACTGTATCTT.............................................	24	0	1	1.00	1	1	0
......TAAAAGATATAGATACACCAAA..............................................................................................................................................................	22	0	1	1.00	1	1	0
..................................................................................................................TGTATCTTTTAGCTGTAACT....................................................	20	0	2	0.50	1	1	0
..................................................................................................................TGTATCTTTTAGCTGTAAC.....................................................	19	0	2	0.50	1	1	0
..................................................................................................................TGTATCTTTTAGCTGTAACTG...................................................	21	0	2	0.50	1	1	0
....................................................................................................................TATCTTTTAGCTGTAACTGTATC...............................................	23	0	2	0.50	1	0	1
..................................................................................................................GGTATCTTTTAGCTGTAACTG...................................................	21	1	2	0.50	1	1	0
.................................................................................................................CTGTATCTTTTAGCTGTAACT....................................................	21	0	2	0.50	1	0	1
....................................................................................................................TATCTTTTAGCTGTAACTGTAT................................................	22	0	2	0.50	1	1	0
................................................................................................................TCTGTATCTTTTAGCTGTAACT....................................................	22	0	2	0.50	1	0	1
................................................................................................................TCTGTATCTTTTAGCTGTAATT....................................................	22	1	2	0.50	1	0	1
...............................................................................................................ATCTGTATCTTTTAGCTGTA.......................................................	20	0	3	0.33	1	1	0
................................................................................................................TCTGTATCTTTTAGCTGTAACTT...................................................	23	1	3	0.33	1	0	1
.................................................................................................................CTGTATCTTTTAGCTGTAACTT...................................................	22	1	3	0.33	1	1	0

TACAACTAAAAGATATAGATACACCAAAAAGATACACTCTAAAGATACAGAGACGGCTGAAAGATACAGATAGAGCAAGCAGATACAGTTAAAAGATACATATTTGAATGCATCTGTATCTTTTAGCTGTAACTGTATCTTTTATATATACAGGTATCTTTTAAAAGTATCAATACCTGCTGTATC
***********************************.....((((((((((..(((((((((((((((((((...(((..((((((..((........)).))))))..))))))))))))))))))))))..))))))))))........************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M046
female body

V110
male body

................................................................................................................TCTGTATCTTTTAGCTGTAACTG...................................................

2.50

..................................................................................................................TGTATCTTTTAGCTGTAACTGT..................................................

2.00

................................................................................................................TCTGTATCTTTTAGCTGTAACTGT..................................................

1.00

..................................................AGACGGCTGAAAGATACAGAT...................................................................................................................

1.00

.......................................................................................................................CTTTTAGCTGTAACTGTATCTT.............................................

1.00

...........................................................................CAAGCAGATACAGTTAAAAGAT.........................................................................................

1.00

...................................................GACGGCTGAAAGATACAGATAGAG...............................................................................................................

1.00

...................................................GACGGCTGAAAGATACAGATAGAGCA.............................................................................................................

1.00

....ACTAAAAGATATAGATACACC.................................................................................................................................................................

1.00

........................................................................................................................TTTTAGCTGTAACTGTATCTT.............................................

1.00

..................................................AGACGGCTGAAAGATACAGA....................................................................................................................

1.00

..............................................................................................................CATCTGTATCTTTTAGCTGTAAC.....................................................

1.00

.....................................................................................................................ATCTTTTAGCTGTAACTGTATCTT.............................................

1.00

......TAAAAGATATAGATACACCAAA..............................................................................................................................................................

1.00

..................................................................................................................TGTATCTTTTAGCTGTAACT....................................................

0.50

..................................................................................................................TGTATCTTTTAGCTGTAAC.....................................................

0.50

..................................................................................................................TGTATCTTTTAGCTGTAACTG...................................................

0.50

....................................................................................................................TATCTTTTAGCTGTAACTGTATC...............................................

0.50

..................................................................................................................GGTATCTTTTAGCTGTAACTG...................................................

0.50

.................................................................................................................CTGTATCTTTTAGCTGTAACT....................................................

0.50

....................................................................................................................TATCTTTTAGCTGTAACTGTAT................................................

0.50

................................................................................................................TCTGTATCTTTTAGCTGTAACT....................................................

0.50

................................................................................................................TCTGTATCTTTTAGCTGTAATT....................................................

0.50

...............................................................................................................ATCTGTATCTTTTAGCTGTA.......................................................

0.33

................................................................................................................TCTGTATCTTTTAGCTGTAACTT...................................................

0.33

.................................................................................................................CTGTATCTTTTAGCTGTAACTT...................................................

0.33

ATGTTGATTTTCTATATCTATGTGGTTTTTCTATGTGAGATTTCTATGTCTCTGCCGACTTTCTATGTCTATCTCGTTCGTCTATGTCAATTTTCTATGTATAAACTTACGTAGACATAGAAAATCGACATTGACATAGAAAATATATATGTCCATAGAAAATTTTCATAGTTATGGACGACATAG **********************************.....((((((((((..(((((((((((((((((((...(((..((((((..((........)).))))))..))))))))))))))))))))))..))))))))))........*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V049 head
..................................................................................TATGTCTATTTTCTAAGTATTA..................................................................................	22	3	9	0.11	1	1

ATGTTGATTTTCTATATCTATGTGGTTTTTCTATGTGAGATTTCTATGTCTCTGCCGACTTTCTATGTCTATCTCGTTCGTCTATGTCAATTTTCTATGTATAAACTTACGTAGACATAGAAAATCGACATTGACATAGAAAATATATATGTCCATAGAAAATTTTCATAGTTATGGACGACATAG
************************************.....((((((((((..(((((((((((((((((((...(((..((((((..((........)).))))))..))))))))))))))))))))))..))))))))))........***********************************

Read size

# Mismatch

Hit Count

Total Norm

Total

V049
head

..................................................................................TATGTCTATTTTCTAAGTATTA..................................................................................

0.11

Species	Coordinate	ID	Alignment
droMoj3	scaffold_6473:2019871-2020056 -	dmo_179	TACAACTAAAAGATATAGATACACCAAAAAGATACACTCTAAAGATACAGAGACGGCTGAAAGATACAGATAGAGCAAGCAGATACAGTTAAAAGATACATATTTGAATGCATCTGTATCTTTTAGCTGTAACTGTATCTTTTATATATACAGGTATCTTTTAAAAGTATCAATACCTGCTGTATC

Species

Coordinate

Alignment

droMoj3

scaffold_6473:2019871-2020056 -

dmo_179

TACAACTAAAAGATATAGATACACCAAAAAGATACACTCTAAAGATACAGAGACGGCTGAAAGATACAGATAGAGCAAGCAGATACAGTTAAAAGATACATATTTGAATGCATCTGTATCTTTTAGCTGTAACTGTATCTTTTATATATACAGGTATCTTTTAAAAGTATCAATACCTGCTGTATC

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droMoj3

TACAACTAAAAGATATAGATACACCAAAAAGATACACTCTAAAGATACAGAGACGGCTGAAAGATACAGATAGAGCAAGCAGATACAGTTAAAAGATACATATTTGAATGCATCTGTATCTTTTAGCTGTAACTGTATCTTTTATATATACAGGTATCTTTTAAAAGTATCAATACCTGCTGTATC	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
................................................................................................................TCTGTATCTTTTAGCTGTAACTG...................................................	23	2	2.50	5	2	0	0	0	0	3
..................................................................................................................TGTATCTTTTAGCTGTAACTGT..................................................	22	2	2.00	4	2	0	0	0	0	2
................................................................................................................TCTGTATCTTTTAGCTGTAACTGT..................................................	24	2	1.00	2	1	0	0	0	0	1
..................................................AGACGGCTGAAAGATACAGAT...................................................................................................................	21	1	1.00	1	0	0	0	0	0	1
.......................................................................................................................CTTTTAGCTGTAACTGTATCTT.............................................	22	1	1.00	1	1	0	0	0	0	0
...........................................................................CAAGCAGATACAGTTAAAAGAT.........................................................................................	22	1	1.00	1	1	0	0	0	0	0
...................................................GACGGCTGAAAGATACAGATAGAG...............................................................................................................	24	1	1.00	1	0	0	0	0	0	1
...................................................GACGGCTGAAAGATACAGATAGAGCA.............................................................................................................	26	1	1.00	1	0	0	0	0	0	1
....ACTAAAAGATATAGATACACC.................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
........................................................................................................................TTTTAGCTGTAACTGTATCTT.............................................	21	1	1.00	1	1	0	0	0	0	0
..................................................AGACGGCTGAAAGATACAGA....................................................................................................................	20	1	1.00	1	0	0	0	0	0	1
..............................................................................................................CATCTGTATCTTTTAGCTGTAAC.....................................................	23	1	1.00	1	0	0	0	0	0	1
.....................................................................................................................ATCTTTTAGCTGTAACTGTATCTT.............................................	24	1	1.00	1	1	0	0	0	0	0
......TAAAAGATATAGATACACCAAA..............................................................................................................................................................	22	1	1.00	1	1	0	0	0	0	0
..................................................................................................................TGTATCTTTTAGCTGTAACT....................................................	20	2	0.50	1	1	0	0	0	0	0
..................................................................................................................TGTATCTTTTAGCTGTAAC.....................................................	19	2	0.50	1	1	0	0	0	0	0
..................................................................................................................TGTATCTTTTAGCTGTAACTG...................................................	21	2	0.50	1	1	0	0	0	0	0
....................................................................................................................TATCTTTTAGCTGTAACTGTATC...............................................	23	2	0.50	1	0	0	0	0	0	1
.................................................................................................................CTGTATCTTTTAGCTGTAACT....................................................	21	2	0.50	1	0	0	0	0	0	1
....................................................................................................................TATCTTTTAGCTGTAACTGTAT................................................	22	2	0.50	1	1	0	0	0	0	0
................................................................................................................TCTGTATCTTTTAGCTGTAACT....................................................	22	2	0.50	1	0	0	0	0	0	1
...............................................................................................................ATCTGTATCTTTTAGCTGTA.......................................................	20	3	0.33	1	1	0	0	0	0	0
................................................................................................................TCTGTATCTTTTAGCTGTAACTT...................................................	23	3	0.33	1	0	0	0	0	0	1
.................................................................................................................CTGTATCTTTTAGCTGTAACTT...................................................	22	3	0.33	1	1	0	0	0	0	0

dmo_179

scaffold_6473:2019921-2020006 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-47.7	-47.4