dmo_1005

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------################################################## AGTTTCCTCTGCTACTTCTGTCTAATATATATAGGTAGAGAACTATTCGACTTTTACGACTTTATTGGATTTGTGGGGCAATTCTTTTTGCTTTGCCTCATTGTGGGCCTAACTGGTGAGTCGTTCAGTTGAGTTCAGATTGAGCTCAAAGTCTGCCAATTGATAATTGGAATATCTGAACAATAACAATTTTTATTGCAGCTCTTATGGGATATTTGCTGTATTTCGTCTATCAGAAATATGTGGAGATG ************************************************.....(((((((..........(((((((((...........))))))))).....(((.....))))))))))(((((((((((((...)))))))))......((((((...)))))).....)))).((((((......))))))...************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M046 female body	V110 male body	M060 embryo	V049 head	V056 head
.........................................................GACTTTATTGGATTTGTGGGGCA...........................................................................................................................................................................	23	0	1	3.00	3	2	1	0	0	0
...........................................................CTTTATTGGATTTGTGGGGCA...........................................................................................................................................................................	21	0	1	3.00	3	1	1	1	0	0
..................................................................................................................................................................................................................GATATTTGCTGTATTTCGT......................	19	0	1	2.00	2	2	0	0	0	0
............................................................TTTATTGGATTTGTGGGGCA...........................................................................................................................................................................	20	0	1	2.00	2	0	1	0	1	0
......................................................TACGACTTTATTGGATTTGTGGGGCA...........................................................................................................................................................................	26	0	1	2.00	2	1	1	0	0	0
.GTTTCCTCTGCTACTTCTGTCT....................................................................................................................................................................................................................................	22	0	1	2.00	2	2	0	0	0	0
.........................................................GACTTTATTGGATTTGTGGGGC............................................................................................................................................................................	22	0	1	2.00	2	2	0	0	0	0
..................................................................................................................................................................................................................GATATTTGCTGTATTTCGTCTAT..................	23	0	1	2.00	2	1	1	0	0	0
.....................................................................................................................................................................................................................................CTATCAGAAATATGTGGAGA..	20	0	1	1.00	1	1	0	0	0	0
.......................AATATATATAGGTAGAGAACTATTC...........................................................................................................................................................................................................	25	0	1	1.00	1	1	0	0	0	0
................................................GACTTTTACGACTTTATTGG.......................................................................................................................................................................................	20	0	1	1.00	1	1	0	0	0	0
...................GTCTAATATATATAGGTAGAGA..................................................................................................................................................................................................................	22	0	1	1.00	1	0	1	0	0	0
.........................................................GACTTTATTGGATTTGTGGGGCAATT........................................................................................................................................................................	26	0	1	1.00	1	0	1	0	0	0
............................................................TTTATTGGATTTGTGGGG.............................................................................................................................................................................	18	0	1	1.00	1	1	0	0	0	0
.GTTTCCTCTGCTACTTCTGTC.....................................................................................................................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0
...................................................................................................................................................................................................................................GTCTATCAGAAATATGTGGA....	20	0	1	1.00	1	1	0	0	0	0
...................GTCTAATATATATAGGTAGAGAACT...............................................................................................................................................................................................................	25	0	1	1.00	1	1	0	0	0	0
.....................................................TTACGACTTTATTGGATTTGCGGGGCA...........................................................................................................................................................................	27	1	1	1.00	1	1	0	0	0	0
.............................................TTCGACTTTTACGACTTTATT.........................................................................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0
.......................................................ACGACTTTATTGGATTTGTGGGGCA...........................................................................................................................................................................	25	0	1	1.00	1	1	0	0	0	0
........................................................CGACTTTATTGGATTTGTGGGGC............................................................................................................................................................................	23	0	1	1.00	1	1	0	0	0	0
...............................................................................AATTCTTTTTGCTTTGCCTC........................................................................................................................................................	20	0	1	1.00	1	1	0	0	0	0
...........................................................CTTTATTGGATTTGTGGGGC............................................................................................................................................................................	20	0	1	1.00	1	1	0	0	0	0
.....................................................................................................................................................................................................................ATTTGCTGTATTTCGTCTAT..................	20	0	1	1.00	1	1	0	0	0	0
....................................................TTTACGACTTTATTGGATTTGTGGGGCA...........................................................................................................................................................................	28	0	1	1.00	1	1	0	0	0	0
.....................................................................................................................................................................................................................................CTATCAGAAATATGTGGAGAT.	21	0	1	1.00	1	1	0	0	0	0
.....................................................TTACGACTTTATTGGATTTGTGGGGCA...........................................................................................................................................................................	27	0	1	1.00	1	0	1	0	0	0
..................................................CTTTTACGACTTTATTGGATTTGTGGGG.............................................................................................................................................................................	28	0	1	1.00	1	1	0	0	0	0
.............ACTTCTGTCTAATATATATAGGTAGAG...................................................................................................................................................................................................................	27	0	1	1.00	1	0	1	0	0	0
.................................................................................................................................................................................................................................TCGTCTATCAGAAATATGTGGAGAT.	25	0	1	1.00	1	1	0	0	0	0
...................GTCTAATATATATAGGTAGA....................................................................................................................................................................................................................	20	0	1	1.00	1	1	0	0	0	0
........................ATATATATAGGTAGAGAACTATTCGAC........................................................................................................................................................................................................	27	0	1	1.00	1	1	0	0	0	0
.....................................................................................................................................................................................................................ATTTGCTGTATTTCGTCTATCAG...............	23	0	1	1.00	1	1	0	0	0	0
.............................................................TTATTGGATTTGTGGGGCAATT........................................................................................................................................................................	22	0	1	1.00	1	1	0	0	0	0
.......................AATATATATAGGTAGAGAACT...............................................................................................................................................................................................................	21	0	1	1.00	1	0	0	1	0	0
.........................................ACTATTCGACTTTTACGACTTTATTGG.......................................................................................................................................................................................	27	0	1	1.00	1	1	0	0	0	0
............................ATATAGGTAGAGAACTATTCGA.........................................................................................................................................................................................................	22	0	1	1.00	1	0	1	0	0	0
..................................................................................................................................................................................................................................CGTCTATCAGAAATATGTGGA....	21	0	1	1.00	1	0	1	0	0	0
.........................................ACTATTCGACTTTTACGACTTT............................................................................................................................................................................................	22	0	1	1.00	1	1	0	0	0	0
................................AGGTAGAGCACTATTGGAC........................................................................................................................................................................................................	19	2	2	0.50	1	0	1	0	0	0
........................................................CGCCATTATTGGATTTGTGG...............................................................................................................................................................................	20	2	2	0.50	1	0	1	0	0	0
.................................................................................................TCATTGTGACACTAACTGGTG.....................................................................................................................................	21	3	2	0.50	1	0	0	0	0	1
............................ATGTAGGTAGAGCACTATTTG..........................................................................................................................................................................................................	21	3	2	0.50	1	0	1	0	0	0
....................................................................................................................................................................................................................................TCTATCAGAAATACGTCGA....	19	2	4	0.50	2	1	1	0	0	0
.................................................................................................TCGCTGTGTGCCTAACTGGTG.....................................................................................................................................	21	3	4	0.25	1	0	0	0	1	0
.................................................................................................TCGCTGTGAGCCTAACTGGT......................................................................................................................................	20	3	5	0.20	1	0	0	0	1	0
................................................................................................................................TTGAGTTCGGATTGCGCT.........................................................................................................	18	2	7	0.14	1	1	0	0	0	0

TCAAAGGAGACGATGAAGACAGATTATATATATCCATCTCTTGATAAGCTGAAAATGCTGAAATAACCTAAACACCCCGTTAAGAAAAACGAAACGGAGTAACACCCGGATTGACCACTCAGCAAGTCAACTCAAGTCTAACTCGAGTTTCAGACGGTTAACTATTAACCTTATAGACTTGTTATTGTTAAAAATAACGTCGAGAATACCCTATAAACGACATAAAGCAGATAGTCTTTATACACCTCTAC ************************************************.....(((((((..........(((((((((...........))))))))).....(((.....))))))))))(((((((((((((...)))))))))......((((((...)))))).....)))).((((((......))))))...************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V049 head	M046 female body
.................................CCATCTCTTGATAAGCTGGAAAT...................................................................................................................................................................................................	23	1	1	1.00	1	0	1
................................................................................................................................TTCTCAAGTCTAACTCGAGT.......................................................................................................	20	2	1	1.00	1	1	0
.....................................................................................................................................................................................................................TAAACCACCAAAAGCAGATA..................	20	3	20	0.15	3	0	3
..............................................................................................................................TCGACTCAAGTTTTACTCGAGT.......................................................................................................	22	3	7	0.14	1	1	0
.........................................................................................................................................................................CTTGTAGACTTTTTCTTGTTA.............................................................	21	3	16	0.06	1	1	0

Species	Coordinate	ID	Alignment
droMoj3	scaffold_6496:6546338-6546588 -	dmo_1005	AGTTTCCTCTGCTACTTCTGTCTAATATATATAGGTAGAGAACTATTCGAC---TTTTACGACTTTATTGGATTTGTGGGGCAATTCTTTTTGCTTTGCCTC--------------ATTGTGGGCCTAACTGGTGAGTCGTT---------------CAGTTGAGTTCAG-----ATTGAGCTCAAAGTCTGCCAATTG-----ATAATTGGAATATCTGAACAATAACA--------------ATTTTTATTGCAGCTCTTATGGGATATTTGCTGTATTTCGTCTATCAGAAATATGTGGAGATG
droVir3	scaffold_12875:19510909-19511169 -	dvi_7977	GGTGTAATGTGCTATTACTGTATGAATTTTATAACTGACGATTTCCTTTATTCATTTTACGACTTT------------GGGGAGTTTCTTTTGGCGTTACTAGTTTTGCTACTTTTAT--TGGCCGCCACAGGTTAGA------------AATTAATCAGCAGCAATCCTAGTCTGTTACGTTCAAAGTCTTTTGTATAAAATTATC----GCTTTAATGACT----------------AATGTACATTAATTCCAGCGTTTCTGGGCTACCTGATTTACTACGTGTACGAAGAGTATGCGAGGCTG
droGri2	scaffold_15245:18034099-18034352 +		GGCGTCCTCTGCTATTGTAATATCGTATATATTACCAATGATCTTTTTGAT---TTTTACGATTTT------------GGATCGTTTTTCTTACTGTTCCTCGGATTTCTTGTTTTGT--TGGCAGCTACAGGTAAGTTGGTAATTGATAAATTAATAA------------------------------CCGCCGA-TG-----ATAAGGAAAATAAATAAATAATAATATAATCTCCAACTATACATTCATTGCAGGGCTTCTGGCCTACATAATGTATTGTGTATACCAAGAGTTTGAGGAATTA

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droMoj3

AGTTTCCTCTGCTACTTCTGTCTAATATATATAGGTAGAGAACTATTCGAC---TTTTACGACTTTATTGGATTTGTGGGGCAATTCTTTTTGCTTTGCCTC--------------ATTGTGGGCCTAACTGGTGAGTCGTT---------------CAGTTGAGTTCAG-----ATTGAGCTCAAAGTCTGCCAATTG-----ATAATTGGAATATCTGAACAATAACA--------------ATTTTTATTGCAGCTCTTATGGGATATTTGCTGTATTTCGTCTATCAGAAATATGTGGAGATG	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
............................................................GACTTTATTGGATTTGTGGGGCA................................................................................................................................................................................................................................	23	1	3.00	3	2	0	0	0	0	1
..............................................................CTTTATTGGATTTGTGGGGCA................................................................................................................................................................................................................................	21	1	3.00	3	1	0	0	0	1	1
..........................................................................................................................................................................................................................................................................GATATTTGCTGTATTTCGT......................	19	1	2.00	2	2	0	0	0	0	0
...............................................................TTTATTGGATTTGTGGGGCA................................................................................................................................................................................................................................	20	1	2.00	2	0	0	1	0	0	1
.........................................................TACGACTTTATTGGATTTGTGGGGCA................................................................................................................................................................................................................................	26	1	2.00	2	1	0	0	0	0	1
.GTTTCCTCTGCTACTTCTGTCT............................................................................................................................................................................................................................................................................................	22	1	2.00	2	2	0	0	0	0	0
............................................................GACTTTATTGGATTTGTGGGGC.................................................................................................................................................................................................................................	22	1	2.00	2	2	0	0	0	0	0
..........................................................................................................................................................................................................................................................................GATATTTGCTGTATTTCGTCTAT..................	23	1	2.00	2	1	0	0	0	0	1
.............................................................................................................................................................................................................................................................................................CTATCAGAAATATGTGGAGA..	20	1	1.00	1	1	0	0	0	0	0
.......................AATATATATAGGTAGAGAACTATTC...................................................................................................................................................................................................................................................................	25	1	1.00	1	1	0	0	0	0	0
................................................GAC---TTTTACGACTTTATTGG............................................................................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
...................GTCTAATATATATAGGTAGAGA..........................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	1
............................................................GACTTTATTGGATTTGTGGGGCAATT.............................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	1
...............................................................TTTATTGGATTTGTGGGG..................................................................................................................................................................................................................................	18	1	1.00	1	1	0	0	0	0	0
.GTTTCCTCTGCTACTTCTGTC.............................................................................................................................................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
...........................................................................................................................................................................................................................................................................................GTCTATCAGAAATATGTGGA....	20	1	1.00	1	1	0	0	0	0	0
...................GTCTAATATATATAGGTAGAGAACT.......................................................................................................................................................................................................................................................................	25	1	1.00	1	1	0	0	0	0	0
.............................................TTCGAC---TTTTACGACTTTATT..............................................................................................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
..........................................................ACGACTTTATTGGATTTGTGGGGCA................................................................................................................................................................................................................................	25	1	1.00	1	1	0	0	0	0	0
...........................................................CGACTTTATTGGATTTGTGGGGC.................................................................................................................................................................................................................................	23	1	1.00	1	1	0	0	0	0	0
..................................................................................AATTCTTTTTGCTTTGCCTC--------------...............................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
..............................................................CTTTATTGGATTTGTGGGGC.................................................................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
.............................................................................................................................................................................................................................................................................ATTTGCTGTATTTCGTCTAT..................	20	1	1.00	1	1	0	0	0	0	0
.......................................................TTTACGACTTTATTGGATTTGTGGGGCA................................................................................................................................................................................................................................	28	1	1.00	1	1	0	0	0	0	0
.............................................................................................................................................................................................................................................................................................CTATCAGAAATATGTGGAGAT.	21	1	1.00	1	1	0	0	0	0	0
........................................................TTACGACTTTATTGGATTTGTGGGGCA................................................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	1
..................................................C---TTTTACGACTTTATTGGATTTGTGGGG..................................................................................................................................................................................................................................	28	1	1.00	1	1	0	0	0	0	0
.............ACTTCTGTCTAATATATATAGGTAGAG...........................................................................................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	1
.........................................................................................................................................................................................................................................................................................TCGTCTATCAGAAATATGTGGAGAT.	25	1	1.00	1	1	0	0	0	0	0
...................GTCTAATATATATAGGTAGA............................................................................................................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
........................ATATATATAGGTAGAGAACTATTCGAC---.............................................................................................................................................................................................................................................................	27	1	1.00	1	1	0	0	0	0	0
.............................................................................................................................................................................................................................................................................ATTTGCTGTATTTCGTCTATCAG...............	23	1	1.00	1	1	0	0	0	0	0
................................................................TTATTGGATTTGTGGGGCAATT.............................................................................................................................................................................................................................	22	1	1.00	1	1	0	0	0	0	0
.......................AATATATATAGGTAGAGAACT.......................................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0
.........................................ACTATTCGAC---TTTTACGACTTTATTGG............................................................................................................................................................................................................................................	27	1	1.00	1	1	0	0	0	0	0
............................ATATAGGTAGAGAACTATTCGA.................................................................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	1
..........................................................................................................................................................................................................................................................................................CGTCTATCAGAAATATGTGGA....	21	1	1.00	1	0	0	0	0	0	1
.........................................ACTATTCGAC---TTTTACGACTTT.................................................................................................................................................................................................................................................	22	1	1.00	1	1	0	0	0	0	0

droVir3

GGTGTAATGTGCTATTACTGTATGAATTTTATAACTGACGATTTCCTTTATTCATTTTACGACTTT------------GGGGAGTTTCTTTTGGCGTTACTAGTTTTGCTACTTTTAT--TGGCCGCCACAGGTTAGA------------AATTAATCAGCAGCAATCCTAGTCTGTTACGTTCAAAGTCTTTTGTATAAAATTATC----GCTTTAATGACT----------------AATGTACATTAATTCCAGCGTTTCTGGGCTACCTGATTTACTACGTGTACGAAGAGTATGCGAGGCTG	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
............................................................GACTTT------------GGGGAGTTTCTTTTGGC....................................................................................................................................................................................................................	23	1	2.00	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......................................................................................................GTTTTGCTACTTTTAT--TGGCCGCC...................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
........................................................................................................................................................TTAATCAGCAGCAATCCT.........................................................................................................................................	18	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................................................TACCTGATTTACTACGTGTACGAAGAGT...........	28	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
......ATGTGCTATTACTGTATG...........................................................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........CTATTACTGTATGAATTTTATAACTGACGATTTCCTTTATT...............................................................................................................................................................................................................................................................	41	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0
.....AATGTGCTATTACTGTATGAATTTT.....................................................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................................TTGCTACTTTTAT--TGGCCGCC...................................................................................................................................................................................	21	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................................CTTTATTCATTTTACGACTTT------------GGGGA................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droGri2

GGCGTCCTCTGCTATTGTAATATCGTATATATTACCAATGATCTTTTTGAT---TTTTACGATTTT------------GGATCGTTTTTCTTACTGTTCCTCGGATTTCTTGTTTTGT--TGGCAGCTACAGGTAAGTTGGTAATTGATAAATTAATAA------------------------------CCGCCGA-TG-----ATAAGGAAAATAAATAAATAATAATATAATCTCCAACTATACATTCATTGCAGGGCTTCTGGCCTACATAATGTATTGTGTATACCAAGAGTTTGAGGAATTA	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

dmo_1005

scaffold_6496:6546388-6546538 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-38.0	-37.9	-37.8