dgr_376

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------################################################## ATGTAAGCTTCGATTTAGTTTACAGTTTTCGTTTATTCTATGGAAGATGGCAATGCCAATTGGAATAGCCTGGAATCTATTTTTACTCGTACTCCTTATGTTAACTAGTTATGTATGCATATATTTAGAGTTCTGGAAATGTAAGTAAGCTTAAATATACAATAATTATTTGTATTTAACGCTTTTGGGGCTCATTCATAGCGCGTTGCACGCAACCTTTTGTGGAGATTGGCGACAATTGTTACTTTCTC **************************************************.((..............((((((((((....((..(((((....................)))))..))....)))).)))))).........))******************************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V109 male body	M041 female body
....................................................ATGCCAATTGGAATAGCCTGG..................................................................................................................................................................................	21	0	1	8.00	8	8	0
.........................................................AATTGGAATAGCCTGGAATCT.............................................................................................................................................................................	21	0	1	2.00	2	1	1
..................................ATTCTATGGAAGATGGCAATG....................................................................................................................................................................................................	21	0	1	2.00	2	2	0
..............................................................................................................................AGAGTTCTGGAAATGTAAGTA........................................................................................................	21	0	1	2.00	2	2	0
...............................................................................TTTTTACTCGTACTCCTTATG.......................................................................................................................................................	21	0	1	2.00	2	0	2
..................................................CAATGCCAATTGGAATAGCCTT...................................................................................................................................................................................	22	1	1	2.00	2	2	0
...................................TTCTATGGAAGATGGCAATGC...................................................................................................................................................................................................	21	0	1	2.00	2	2	0
.....................ACAGTTTTCGTTTATTCTATG.................................................................................................................................................................................................................	21	0	1	1.00	1	0	1
...............................................................................................................TGTATGCATATATTTAGAGTT.......................................................................................................................	21	0	1	1.00	1	1	0
.............................................................................................CCTTATGTTATCTAGTTATG..........................................................................................................................................	20	1	1	1.00	1	0	1
..................................................CAATGCCAATTGGAATAGCCTG...................................................................................................................................................................................	22	0	1	1.00	1	1	0
..............................GTTTATTCTATGGAATATGGC........................................................................................................................................................................................................	21	1	1	1.00	1	1	0
..........................TTTCGTTTATTCTATGGAAGA............................................................................................................................................................................................................	21	0	1	1.00	1	1	0
............................TCGTTTATTCTATGGAAGATG..........................................................................................................................................................................................................	21	0	1	1.00	1	0	1
..................................................CAATGCCAATTGGAATAACC.....................................................................................................................................................................................	20	1	1	1.00	1	1	0
.......CTTCGATTTAGTTTACAGTTT...............................................................................................................................................................................................................................	21	0	1	1.00	1	0	1
.........................................................AATTGGAATAGCCTGGAATCTATTTTTA......................................................................................................................................................................	28	0	1	1.00	1	1	0
............................................................................................................................TTAGAGTTCTGGAAATGTAAG..........................................................................................................	21	0	1	1.00	1	1	0
......................................................................................................................................................................................................................ACCTTTTGTGGAGATTGGCGA................	21	0	1	1.00	1	1	0
...............................................................................................................TGTATGCATCTATTTAGAGTT.......................................................................................................................	21	1	1	1.00	1	0	1
.........................TTTTCGTTTATTCTATGGAAG.............................................................................................................................................................................................................	21	0	1	1.00	1	0	1
....................................................................................................TTAACTAGTTATGTATGCATC..................................................................................................................................	21	1	1	1.00	1	1	0
...........................................................................................................................................................................GTATTTAACGCTTTTGGGGCTT..........................................................	22	1	1	1.00	1	0	1
...................................................................................................GTTATCTAGTTATGTATGCAT...................................................................................................................................	21	1	1	1.00	1	0	1
...................................................................................TACTCGTACTCCTTATGTTAA...................................................................................................................................................	21	0	1	1.00	1	0	1
............................................................................................................................TTAGAGTTCTGGAAATGTAAGTAAGCT....................................................................................................	27	0	1	1.00	1	1	0
.............................CGTTTATTCTATGGAAGATGG.........................................................................................................................................................................................................	21	0	1	1.00	1	1	0
..............................................................................................................................AGAGTTCTGGAAATGTAAGTAAGCTT...................................................................................................	26	0	1	1.00	1	1	0
..............................GTTTATTCTATGGAAGATGGC........................................................................................................................................................................................................	21	0	1	1.00	1	1	0
..............................................ATGGCAATGCCAATTGGAATAGCC.....................................................................................................................................................................................	24	0	1	1.00	1	0	1
.............................................................................................................................TAGAGTTCTGGAAATGTAAGT.........................................................................................................	21	0	1	1.00	1	1	0
..........................................................ATTGGAATAGCCTGGAATC..............................................................................................................................................................................	19	0	1	1.00	1	0	1
................................................................................................................GTATGCATCTATTTAGAGTT.......................................................................................................................	20	1	1	1.00	1	0	1
...........................................................TTGGAATAGCCTGGAATCTAT...........................................................................................................................................................................	21	0	1	1.00	1	1	0
...................................................AATGCCAATTGGAATAGCCTG...................................................................................................................................................................................	21	0	1	1.00	1	1	0
.......................................ATGGAAGATGGCAATGCCAATTA.............................................................................................................................................................................................	23	1	1	1.00	1	0	1
....................................TCTATGGAAGATGGCAATGC...................................................................................................................................................................................................	20	0	1	1.00	1	1	0
...............................................................................................................................GAGTTCTGGAAATGTAAGTAAGCTTA..................................................................................................	26	0	1	1.00	1	1	0
..............................GTTTAGTCTATGGAAGATGGC........................................................................................................................................................................................................	21	1	1	1.00	1	1	0
...................................................................................................................TGCATCTATTTAGAGTTCTGG...................................................................................................................	21	1	1	1.00	1	0	1
..........................................................................................................................ATTTAGAGTTCTGGAAATGTA............................................................................................................	21	0	1	1.00	1	1	0
................................................................................................................................................................................................................CACGCAACCTTTTGTGGAGATTGGC..................	25	0	1	1.00	1	0	1
..................TTTACAGTTTTCGTTTATTCT....................................................................................................................................................................................................................	21	0	1	1.00	1	0	1

TACATTCGAAGCTAAATCAAATGTCAAAAGCAAATAAGATACCTTCTACCGTTACGGTTAACCTTATCGGACCTTAGATAAAAATGAGCATGAGGAATACAATTGATCAATACATACGTATATAAATCTCAAGACCTTTACATTCATTCGAATTTATATGTTATTAATAAACATAAATTGCGAAAACCCCGAGTAAGTATCGCGCAACGTGCGTTGGAAAACACCTCTAACCGCTGTTAACAATGAAAGAG ******************************************************************************************************.((..............((((((((((....((..(((((....................)))))..))....)))).)))))).........))**************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M041 female body	V109 male body
.............................................................................ATAAAAATGAGCATGAGGAAT.........................................................................................................................................................	21	0	1	2.00	2	1	1
...ATTCGAAGCTAAATCAAATGT...................................................................................................................................................................................................................................	21	0	1	2.00	2	1	1
............................................................................................AGGAATACAATTGATCAATA...........................................................................................................................................	20	0	1	1.00	1	1	0
...............................................ACCGTTACGGTTAACCTTATC.......................................................................................................................................................................................	21	0	1	1.00	1	1	0
..................................TAAGATACCTTCTACCGTTAC....................................................................................................................................................................................................	21	0	1	1.00	1	1	0
...........................................................................................GAGGAATACAATTGATCAATA...........................................................................................................................................	21	0	1	1.00	1	0	1
............................................................................................AGGAATACAATTGATCAATAC..........................................................................................................................................	21	0	1	1.00	1	1	0
......................................................................................................TTGATCAATACATACGTAGAT................................................................................................................................	21	1	1	1.00	1	1	0
..........................................................................................TGAGGAATACAATTGATCAAT............................................................................................................................................	21	0	1	1.00	1	1	0
............................................................................................................................................................................................................................ACACCTCTAACCGCTGTTAAC..........	21	0	1	1.00	1	1	0

Species	Coordinate	ID	Alignment
droGri2	scaffold_15126:180324-180574 -	dgr_376	ATGTAAGCTTCGATTTAGTTTACAGTTTTCGTTTATTCTATGGAAGATGGCAATGCCAATTGGAATAGCCTGGAATCTATTTTTACTCGTA--CTCCTTATGTTAACTAGTTATGTATGCATATATTTAGAGTTCTGGAAATGTAAGTAAGCTT-----------------------------------AAATATACAATAATTATTTGTATTTAACGCTTTTGGGGC-------TCATT-CATAGCGCGTTGCACGCAACCTTTTGTGGAGATTGGCGACAATTGTTACTTTCTC
droVir3	scaffold_12723:3292973-3293214 -	dvi_1462	TTGAGCCCTTCGCTTGGCAGCACAGTTTCCATTCGC---ATAGAAGATGATGGCGTCG---GGTAGGTCCTGCGAGCTGGTGATACTTGTT--TTGCTCATATTGGCCAGTTATGCCTGCATCTACTTGGAATTCTGGAAACGTAAGCTCTGCTGTTCTGC-----------TGCTGGTTAA----------------CTTATCTCA-------------TTTGGTCC----TCT-CACC-CACAGCCCGCTGTATGAAGCCCTTCGTGGAGATCGGCGACAATTGCTATTTCTTT
droMoj3	scaffold_6500:6499470-6499709 -		TTGAGCCCTACGATACGCCTCGCAGTTTAGCTCTGG---ATGGAACATGATGATGTTT---GGCAAGTCTTGTGAGCTGGTAATACTTGTG--CTCCTCATATTGGCCAGCTATGCTTGCTTCTACTTGGAGTTCTGGAAACGTAAGTTCAGTCGGTCT------------------------------ATGAATTCAGTTATTGCC-------------TTTGCTTC----CCTGTTCA-AGCAGCCCGTTGTATGACACCCTTTGTGGAAATCGGCGACAACTGCTACTTTTTC
droWil2	scf2_1100000004516:90835-90988 -		G-------------------------------------------------------------------------------------------------------------CTATGTCTGTATCTATCAAGAATACTTTCAATGTAAGTAAGCCT-------------------------TAAATGATTTGCAATTTATTTTATTTTT-------TATACTTTTATGTATATATATATATT-TTAAGCCGCTTGTTCCAAACCCTTTGTACAGATTGGCCGAAATTGTTATTTTTTC
dp5	XL_group1e:7122339-7122421 +		AT--------TGATTGT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ATTTATGGTCT----TATGCCTT-CCCAGCTTACTGCTCGAAGCCCTTTGTGAACATCGGTGGACGGTGCTATTTCTTC
droPer2	scaffold_13:1431473-1431561 -		TTAAGTA--TAGATTGT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ATTTATGGTCT----TATGCCTT-CCCAGCTCACTGCTCGAAGCCCTTTGTGAACATCGGTGGACGGTGCTATTTCTTC
droAna3	scaffold_12916:5060878-5060935 -		AC---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-AACAGCTCGCTGTAGCAGGCCCTTTGTGCAAATCGGAGATCGTTGCTACTTCTTC
droBip1	scf7180000396366:160000-160057 -		AT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-AACAGCTCGCTGTACCAAGCCGTTTGTGCAGATTGGGAATCGTTGCTACTTTTTC
droKik1	scf7180000302684:979967-980122 +		TA-------------------------------------------------------------------------------------------CTGCTCCTGTTGTTTAGTTATATATACATTTTCAAGGAATTTTCCAAGCGTGAGTTGTAATAG--TTG-----------TAATATTCAA----------------AT---T-------------GTTTATGGTGC----TATTCCTAAGCCAGCTTTATGTTCAAAGCCCTTTGTAAACATTGGTGAACGTTGCTTTTTCTTC
droEle1	scf7180000491046:3617384-3617436 -		T---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGCTCTCTGCTCTAAACCTTTTGTGAAGATTGGAGAACGCTGTTACTTCTTT
droBia1	scf7180000302422:4276999-4277051 -		T---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGCTCTCTGTACAAAGCCCTTTGTGAAGATTGGCGATCGCTGCTATTTCTTT
droTak1	scf7180000414060:1814-2040 +		TATAGCT--TCAGTT-AATTGAGAGC-------CG--CTGTGAAAAATGTCAGCGCTA---GGAAAATCTTTC--GTCGGTCTTACAGGTTTTTTAATCCTGACTGCCGGATACGTTTACATTTTCAAGAACTGCTGGCAACGTGAGTTTGGAAACTATACTTCGTAATATTTCAAATTCAA----------------TT----------------------------------TATTAC-CCTAGCTCTTTGTTCAAAACCCTTTGTGAAGATTGGCGAACGCTGCTATTTTTT-
droEug1	scf7180000409463:1678394-1678545 +		TA-------------------------------------------------------------------------------------------CTACTCCTGACGATCGGATATATCTACATCTTCAAGGACTGTTGGCAACGTAAACTTTGTGTAAATTT-----------TAAAAATCAA----------------T---------------------TTTGAATA----TTTAATAT-CCCAGCTCGCTGCTCGAAGCCCTTTGTTGAGATTGGAGATCGCTGCTATTTCTTT

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droGri2

ATGTAAGCTTCGATTTAGTTTACAGTTTTCGTTTATTCTATGGAAGATGGCAATGCCAATTGGAATAGCCTGGAATCTATTTTTACTCGTA--CTCCTTATGTTAACTAGTTATGTATGCATATATTTAGAGTTCTGGAAATGTAAGTAAGCTT-----------------------------------AAATATACAATAATTATTTGTATTTAACGCTTTTGGGGC-------TCATT-CATAGCGCGTTGCACGCAACCTTTTGTGGAGATTGGCGACAATTGTTACTTTCTC	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
....................................................ATGCCAATTGGAATAGCCTGG...............................................................................................................................................................................................................................	21	1	8.00	8	0	8
.........................................................AATTGGAATAGCCTGGAATCT..........................................................................................................................................................................................................................	21	1	2.00	2	1	1
..................................ATTCTATGGAAGATGGCAATG.................................................................................................................................................................................................................................................	21	1	2.00	2	0	2
................................................................................................................................AGAGTTCTGGAAATGTAAGTA...................................................................................................................................................	21	1	2.00	2	0	2
...............................................................................TTTTTACTCGTA--CTCCTTATG..................................................................................................................................................................................................	21	1	2.00	2	2	0
..................................................CAATGCCAATTGGAATAGCCTT................................................................................................................................................................................................................................	22	1	2.00	2	0	2
...................................TTCTATGGAAGATGGCAATGC................................................................................................................................................................................................................................................	21	1	2.00	2	0	2
.....................ACAGTTTTCGTTTATTCTATG..............................................................................................................................................................................................................................................................	21	1	1.00	1	1	0
.................................................................................................................TGTATGCATATATTTAGAGTT..................................................................................................................................................................	21	1	1.00	1	0	1
..................................................CAATGCCAATTGGAATAGCCTG................................................................................................................................................................................................................................	22	1	1.00	1	0	1
..........................TTTCGTTTATTCTATGGAAGA.........................................................................................................................................................................................................................................................	21	1	1.00	1	0	1
............................TCGTTTATTCTATGGAAGATG.......................................................................................................................................................................................................................................................	21	1	1.00	1	1	0
.......CTTCGATTTAGTTTACAGTTT............................................................................................................................................................................................................................................................................	21	1	1.00	1	1	0
.........................................................AATTGGAATAGCCTGGAATCTATTTTTA...................................................................................................................................................................................................................	28	1	1.00	1	0	1
..............................................................................................................................TTAGAGTTCTGGAAATGTAAG.....................................................................................................................................................	21	1	1.00	1	0	1
...................................................................................................................................................................................................................................................................ACCTTTTGTGGAGATTGGCGA................	21	1	1.00	1	0	1
.........................TTTTCGTTTATTCTATGGAAG..........................................................................................................................................................................................................................................................	21	1	1.00	1	1	0
......................................................................................................TTAACTAGTTATGTATGCATC.............................................................................................................................................................................	21	1	1.00	1	0	1
................................................................................................................................................................................................................GTATTTAACGCTTTTGGGGC-------TT...........................................................	22	1	1.00	1	1	0
...................................................................................TACTCGTA--CTCCTTATGTTAA..............................................................................................................................................................................................	21	1	1.00	1	1	0
..............................................................................................................................TTAGAGTTCTGGAAATGTAAGTAAGCT...............................................................................................................................................	27	1	1.00	1	0	1
.............................CGTTTATTCTATGGAAGATGG......................................................................................................................................................................................................................................................	21	1	1.00	1	0	1
................................................................................................................................AGAGTTCTGGAAATGTAAGTAAGCTT-----------------------------------...........................................................................................................	26	1	1.00	1	0	1
..............................GTTTATTCTATGGAAGATGGC.....................................................................................................................................................................................................................................................	21	1	1.00	1	0	1
..............................................ATGGCAATGCCAATTGGAATAGCC..................................................................................................................................................................................................................................	24	1	1.00	1	1	0
...............................................................................................................................TAGAGTTCTGGAAATGTAAGT....................................................................................................................................................	21	1	1.00	1	0	1
..........................................................ATTGGAATAGCCTGGAATC...........................................................................................................................................................................................................................	19	1	1.00	1	1	0
...........................................................TTGGAATAGCCTGGAATCTAT........................................................................................................................................................................................................................	21	1	1.00	1	0	1
...................................................AATGCCAATTGGAATAGCCTG................................................................................................................................................................................................................................	21	1	1.00	1	0	1
.......................................ATGGAAGATGGCAATGCCAATTA..........................................................................................................................................................................................................................................	23	1	1.00	1	1	0
....................................TCTATGGAAGATGGCAATGC................................................................................................................................................................................................................................................	20	1	1.00	1	0	1
.................................................................................................................................GAGTTCTGGAAATGTAAGTAAGCTT-----------------------------------A..........................................................................................................	26	1	1.00	1	0	1
............................................................................................................................ATTTAGAGTTCTGGAAATGTA.......................................................................................................................................................	21	1	1.00	1	0	1
.............................................................................................................................................................................................................................................................CACGCAACCTTTTGTGGAGATTGGC..................	25	1	1.00	1	1	0
..................TTTACAGTTTTCGTTTATTCT.................................................................................................................................................................................................................................................................	21	1	1.00	1	1	0

droVir3

TTGAGCCCTTCGCTTGGCAGCACAGTTTCCATTCGC---ATAGAAGATGATGGCGTCG---GGTAGGTCCTGCGAGCTGGTGATACTTGTT--TTGCTCATATTGGCCAGTTATGCCTGCATCTACTTGGAATTCTGGAAACGTAAGCTCTGCTGTTCTGC-----------TGCTGGTTAA----------------CTTATCTCA-------------TTTGGTCC----TCT-CACC-CACAGCCCGCTGTATGAAGCCCTTCGTGGAGATCGGCGACAATTGCTATTTCTTT	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
..........................TTCCATTCGC---ATAGAAGATGA......................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................................................ATGAAGCCCTTCGTGGAGATCGGC..................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................CGC---ATAGAAGATGATGGC..................................................................................................................................................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................TGATGGCGTCG---GGTAGGTCCT.................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................................................................................CTACTTGGAATTCTGGAAACCC........................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................CGC---ATAGAAGATGATGGCGTC...............................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................................................................................CTACTTGGAATTCTGGAAACCCC.......................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.......................................................................GCGAGCTGGTGATACTTGTT--TTGCT......................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
............................................................................................................................................................................................................................................................................GGAGATCGGCGACAATTGCTAT......	22	1	1.00	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................................................................................................................CTTGGAATTCTGGAAACC.........................................................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..........................................................................................................................CTACTTGGAATTCTGGAA............................................................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0
..........................TTCCATTCGC---ATAGAAGATGATGGC..................................................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................................................................................................................CGACAATTGCTATTTCTT.	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................TGATGGCGTCG---GGTAGGTCCTGC...............................................................................................................................................................................................................................	23	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................................................................................GCTGTATGAAGCCCTTCGT............................	19	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.........................TTTCCATTCGC---ATAGAAGAT........................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...............................................TGATGGCGTCG---GGTAGGTCCTGCGAGC...........................................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................................................................GCTGGTGATACTTGTT--TTGCT......................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................................................AGCCCTTCGTGGAGATCG....................	18	1	1.00	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
................................................GATGGCGTCG---GGTAGGTCCTGCGA.............................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
...........................TCCATTCGC---ATAGAAGATGATGGCGTC...............................................................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.............................................................................................................................CTTGGAATTCTGGAAACCCC.......................................................................................................................................................	20	6	0.17	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droMoj3

TTGAGCCCTACGATACGCCTCGCAGTTTAGCTCTGG---ATGGAACATGATGATGTTT---GGCAAGTCTTGTGAGCTGGTAATACTTGTG--CTCCTCATATTGGCCAGCTATGCTTGCTTCTACTTGGAGTTCTGGAAACGTAAGTTCAGTCGGTCT------------------------------ATGAATTCAGTTATTGCC-------------TTTGCTTC----CCTGTTCA-AGCAGCCCGTTGTATGACACCCTTTGTGGAAATCGGCGACAACTGCTACTTTTTC	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
............................................................................................................AGCTATGCTTGCTTCTACTTGGA.....................................................................................................................................................................	23	1	2.00	2	2	0	0	0	0	0
................................................................................................................................................AAGTTCAGTCGGTCT------------------------------ATGAATTCAGT................................................................................................	26	1	1.00	1	1	0	0	0	0	0
.............................GCTCTGG---ATGGAACATGATGATGT................................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	0	1
.......................AGTTTAGCTCTGG---ATGGAAC..........................................................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	1
......................................................................................................................................................................................................................................................................CTTTGTGGAAATCGGCGACAACTG..........	24	1	1.00	1	0	0	0	0	0	1
................................................................................................................................................................................................................................................................GACACCCTTTGTGGAAATC.....................	19	1	1.00	1	1	0	0	0	0	0
.........................TTTAGCTCTGG---ATGGAACATG.......................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	1
....GCCCTACGATACGCCTCGCA................................................................................................................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
..................................................................................................CATATTGGCCAGCTATGCTTG.................................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
................................CTGG---ATGGAACATGATGATGTTT---GGCA.......................................................................................................................................................................................................................................	27	1	1.00	1	1	0	0	0	0	0
............................................................................................................AGCTATGCTTGCTTCTACTTG.......................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
....................................................................................ACTTGTG--CTCCTCATATTGGCCAGCT........................................................................................................................................................................................	26	1	1.00	1	1	0	0	0	0	0
.................................................................................................................TGCTTGCTTCTACTTGGAGTTCTG...............................................................................................................................................................	24	1	1.00	1	1	0	0	0	0	0
............................................................................................................................................................................................................................................................GTATGACACCCTTTGTGGAAATCGGCGA................	28	1	1.00	1	1	0	0	0	0	0
........................GTTTAGCTCTGG---ATGGAAC..........................................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	1
.............................................CATGATGATGTTT---GGCAA......................................................................................................................................................................................................................................	18	1	1.00	1	1	0	0	0	0	0
...............................................................CAAGTCTTGTGAGCTGGTA......................................................................................................................................................................................................................	19	1	1.00	1	1	0	0	0	0	0
..GAGCCCTACGATACGCCTCGCTC...............................................................................................................................................................................................................................................................................	23	1	1.00	1	1	0	0	0	0	0

droWil2

G-------------------------------------------------------------------------------------------------------------CTATGTCTGTATCTATCAAGAATACTTTCAATGTAAGTAAGCCT-------------------------TAAATGATTTGCAATTTATTTTATTTTT-------TATACTTTTATGTATATATATATATT-TTAAGCCGCTTGTTCCAAACCCTTTGTACAGATTGGCCGAAATTGTTATTTTTTC	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
..........................................................................................................................................................................................................................................................................GTACAGATTGGCCGAAATT...........	19	1	2.00	2	0	0	2	0	0
..........................................................................................................................................................................................................................................................................GTACAGATTGGCCGAAATTGT.........	21	1	1.00	1	0	0	1	0	0
......................................................................................................................................................................................................................................................CCGCTTGTTCCAAACCCTTTGTACA.........................	25	1	1.00	1	0	1	0	0	0
...............................................................................................................................................................................................................................................................................GATTGGCCGAAATTGTTATTTT...	22	1	1.00	1	0	0	1	0	0
..............................................................................................................CTATGTCTGTATCTATCAAGA.....................................................................................................................................................................	21	1	1.00	1	0	1	0	0	0

dp5

AT--------TGATTGT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ATTTATGGTCT----TATGCCTT-CCCAGCTTACTGCTCGAAGCCCTTTGTGAACATCGGTGGACGGTGCTATTTCTTC

Size

Hit Count

Total Norm

Total

M022
Male-body

M040
Female-body

M059
Embryo

M062
Head

V043
Embryo

V051
Head

V112
Male-body

SRR902010
Ovary

SRR902011
Testis

SRR902012
CNS imaginal disc

No Reads

droPer2

TTAAGTA--TAGATTGT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ATTTATGGTCT----TATGCCTT-CCCAGCTCACTGCTCGAAGCCCTTTGTGAACATCGGTGGACGGTGCTATTTCTTC	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
No Reads

droAna3

AC---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-AACAGCTCGCTGTAGCAGGCCCTTTGTGCAAATCGGAGATCGTTGCTACTTCTTC	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
.....................................................................................................................................................................................................................................................GCTCGCTGTAGCAGGCCCTTTGTG...........................	24	1	1.00	1	1	0	0	0	0	0

droBip1

AT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-AACAGCTCGCTGTACCAAGCCGTTTGTGCAGATTGGGAATCGTTGCTACTTTTTC	Size	Hit Count	Total Norm	Total
No Reads

droKik1

TA-------------------------------------------------------------------------------------------CTGCTCCTGTTGTTTAGTTATATATACATTTTCAAGGAATTTTCCAAGCGTGAGTTGTAATAG--TTG-----------TAATATTCAA----------------AT---T-------------GTTTATGGTGC----TATTCCTAAGCCAGCTTTATGTTCAAAGCCCTTTGTAAACATTGGTGAACGTTGCTTTTTCTTC	Size	Hit Count	Total Norm	Total
No Reads

droEle1

T---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGCTCTCTGCTCTAAACCTTTTGTGAAGATTGGAGAACGCTGTTACTTCTTT	Size	Hit Count	Total Norm	Total
No Reads

droBia1

T---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGCTCTCTGTACAAAGCCCTTTGTGAAGATTGGCGATCGCTGCTATTTCTTT	Size	Hit Count	Total Norm	Total
No Reads

droTak1

TATAGCT--TCAGTT-AATTGAGAGC-------CG--CTGTGAAAAATGTCAGCGCTA---GGAAAATCTTTC--GTCGGTCTTACAGGTTTTTTAATCCTGACTGCCGGATACGTTTACATTTTCAAGAACTGCTGGCAACGTGAGTTTGGAAACTATACTTCGTAATATTTCAAATTCAA----------------TT----------------------------------TATTAC-CCTAGCTCTTTGTTCAAAACCCTTTGTGAAGATTGGCGAACGCTGCTATTTTTT-	Size	Hit Count	Total Norm	Total
No Reads

droEug1

TA-------------------------------------------------------------------------------------------CTACTCCTGACGATCGGATATATCTACATCTTCAAGGACTGTTGGCAACGTAAACTTTGTGTAAATTT-----------TAAAAATCAA----------------T---------------------TTTGAATA----TTTAATAT-CCCAGCTCGCTGCTCGAAGCCCTTTGTTGAGATTGGAGATCGCTGCTATTTCTTT	Size	Hit Count	Total Norm	Total
No Reads

-10.2	-10.0	-9.9	-9.7

dgr_376

scaffold_15126:180374-180524 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads