dgr_360

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------################################################## AAGTGGAGGGGAATGTGTTCGAAAAGATGAGAAACTGTCGTATATATCTCTTAAATTTGACAACAAAACAGAAATACGTTTTCAGATCAAGGATATCTGCAGAAAAGTTGAGCAATTGCAGCTCGAAGAGATTAGGTTTGTATTGGAAACCCGACATGTATTTCAAAAAAATTTGAAAGATTCTTATCACCACATTTGCAGGCTAGAACTGAATCGTCAGAGCGAATTGCAGAATCGTCAGATCGATTTGC ************************************************....((((((.(((.............))).)))))).........(((((((....((((((.......))))))........(((((.......))))).........(((((((....)))))))(((....)))......)))))))************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M041 female body	V109 male body
.........................................................................ATTGGTTTTCAGATCAAGG...............................................................................................................................................................	19	2	5	3.20	16	16	0
.AGTGGAGGGGAATGTGTTC.......................................................................................................................................................................................................................................	19	0	3	1.67	5	1	4
.........................................................................ATTGGTTTTCAGATCAAG................................................................................................................................................................	18	2	19	1.16	22	22	0
.................................................................................................................AATTGCAGCTCGAAGAGATTAG....................................................................................................................	22	0	3	1.00	3	0	3
.AGTGGAGGGGAATGTGTT........................................................................................................................................................................................................................................	18	0	3	1.00	3	1	2
.AGTGGAGGGGAATGTGTTCG......................................................................................................................................................................................................................................	20	0	3	1.00	3	2	1
AAGTGGAGGGGAATGTGTTC.......................................................................................................................................................................................................................................	20	0	3	1.00	3	1	2
.............................................................................................................................................................................................................GAACTGAATCGTCAGAGCG...........................	19	0	3	0.67	2	0	2
...............................................................................................................................................................................................................................GAATTGCAGAATCGTCAGATC.......	21	0	2	0.50	1	1	0
...............................................................................................................................................................................................................................GAATTGCAGAATCGTCAGATCGA.....	23	0	2	0.50	1	0	1
..........................................................................................................................................................................................................................AGAGCGAATTGCAGAATCGTC............	21	0	2	0.50	1	1	0
................................................................................................................................................................................................................CTGAATCGTCAGAGCGAATTGCAGAATC...............	28	0	2	0.50	1	1	0
..............................................................................................................................................................................................................................CGAATTGCAGAATCGTCAGATC.......	22	0	2	0.50	1	1	0
.......................................................................................................................................................................................................................GTCAGAGCGAATTGCAGAATCGTCAGA.........	27	0	2	0.50	1	0	1
................................................................................................................................................................................................................CTGAATCGTCAGAGCGAATTGCA....................	23	0	2	0.50	1	1	0
................................................................................................................................................................................................................................AATTGCAGAATCGTCAGATCGATTTG.	26	0	2	0.50	1	0	1
.....................................................................................................................GCAGCTCGAAGAGATTAGG...................................................................................................................	19	0	3	0.33	1	1	0
........................................................................................................AAGTTGAGCAATTGCAGCT................................................................................................................................	19	0	3	0.33	1	1	0
.AGTGGAGGGGAATGTGTTCGA.....................................................................................................................................................................................................................................	21	0	3	0.33	1	0	1
.....................AAAAGATGAGAAACTGTCGTATATA.............................................................................................................................................................................................................	25	0	3	0.33	1	0	1
.................................................................................................................................................................................................................TGGATCGTCGGAGCGAATT.......................	19	2	3	0.33	1	1	0
............................................................................................................................................................................................................AGAACTGAATCGTCAGAGC............................	19	0	3	0.33	1	1	0
.....................................................................................................................GCAGCTCGAAGAGATTAGGCTAG...............................................................................................................	23	2	3	0.33	1	0	1
.............................AGAAACTGTCGTATATATCTCTTA......................................................................................................................................................................................................	24	0	3	0.33	1	0	1
..................................................................................................................ATTGCAGCTCGAAGAGATTAGGCTA................................................................................................................	25	2	3	0.33	1	1	0
........................AGATGAGAAACTGTCGTATATATCT..........................................................................................................................................................................................................	25	0	3	0.33	1	0	1
...............................................................................................................................................................................................................CCTGGATCGTCGGAGCGAATT.......................	21	3	3	0.33	1	1	0
AAGTGGAGGGGAATGTGTTCG......................................................................................................................................................................................................................................	21	0	3	0.33	1	0	1
.................TTCGAAAAGATGAGAAACT.......................................................................................................................................................................................................................	19	0	3	0.33	1	1	0
..................................................................................................................................................................................................................GAATCGTCAGAGCGAATT.......................	18	0	3	0.33	1	1	0
................GTTCGAAAAGATGAGAAACTGTCGT..................................................................................................................................................................................................................	25	0	3	0.33	1	0	1
.....................................................................................................................GCAGCTCGAAGAGATTAGGCT.................................................................................................................	21	1	3	0.33	1	1	0
.....................................................................................................................................................................................................TTAGGCTAGAACTGAATCGTC.................................	21	2	3	0.33	1	0	1
..GTGGAGGGGAATGTGTTCG......................................................................................................................................................................................................................................	19	0	3	0.33	1	0	1
AAGTGGAGGGGAATGTGTT........................................................................................................................................................................................................................................	19	0	3	0.33	1	0	1
....................GAAAAGATGAGAAACTGTCG...................................................................................................................................................................................................................	20	0	3	0.33	1	1	0
........................................................................CATTGGTTTTCAGATCAAG................................................................................................................................................................	19	3	20	0.30	6	6	0
....................................................................................................................................................................................................................................GCAGAATCGTCAGATCGATT...	20	0	4	0.25	1	1	0
.............................................................................GTTTTCAGATCAAGAGTA............................................................................................................................................................	18	2	9	0.22	2	0	2
...............................................................................................................................................................................................................CCTGGATCGTCGGAGCGAA.........................	19	3	7	0.14	1	1	0
.............................................................................GTTTTCAGATCAAGGTAATG..........................................................................................................................................................	20	3	7	0.14	1	0	1
.........................................................................ATTGGTTTTCAGATCAAGGT..............................................................................................................................................................	20	3	16	0.06	1	0	1

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------##################################################
AAGTGGAGGGGAATGTGTTCGAAAAGATGAGAAACTGTCGTATATATCTCTTAAATTTGACAACAAAACAGAAATACGTTTTCAGATCAAGGATATCTGCAGAAAAGTTGAGCAATTGCAGCTCGAAGAGATTAGGTTTGTATTGGAAACCCGACATGTATTTCAAAAAAATTTGAAAGATTCTTATCACCACATTTGCAGGCTAGAACTGAATCGTCAGAGCGAATTGCAGAATCGTCAGATCGATTTGC
**************************************************....((((((.(((.............))).)))))).........(((((((....((((((.......))))))........(((((.......))))).........(((((((....)))))))(((....)))......)))))))**************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M041
female body

V109
male body

.........................................................................ATTGGTTTTCAGATCAAGG...............................................................................................................................................................

3.20

.AGTGGAGGGGAATGTGTTC.......................................................................................................................................................................................................................................

1.67

.........................................................................ATTGGTTTTCAGATCAAG................................................................................................................................................................

1.16

.................................................................................................................AATTGCAGCTCGAAGAGATTAG....................................................................................................................

1.00

.AGTGGAGGGGAATGTGTT........................................................................................................................................................................................................................................

1.00

.AGTGGAGGGGAATGTGTTCG......................................................................................................................................................................................................................................

1.00

AAGTGGAGGGGAATGTGTTC.......................................................................................................................................................................................................................................

1.00

.............................................................................................................................................................................................................GAACTGAATCGTCAGAGCG...........................

0.67

0.50

.....................................................................................................................GCAGCTCGAAGAGATTAGG...................................................................................................................

0.33

........................................................................................................AAGTTGAGCAATTGCAGCT................................................................................................................................

0.33

.AGTGGAGGGGAATGTGTTCGA.....................................................................................................................................................................................................................................

0.33

.....................AAAAGATGAGAAACTGTCGTATATA.............................................................................................................................................................................................................

0.33

.....................................................................................................................GCAGCTCGAAGAGATTAGGCTAG...............................................................................................................

0.33

.............................AGAAACTGTCGTATATATCTCTTA......................................................................................................................................................................................................

0.33

..................................................................................................................ATTGCAGCTCGAAGAGATTAGGCTA................................................................................................................

0.33

........................AGATGAGAAACTGTCGTATATATCT..........................................................................................................................................................................................................

0.33

AAGTGGAGGGGAATGTGTTCG......................................................................................................................................................................................................................................

0.33

.................TTCGAAAAGATGAGAAACT.......................................................................................................................................................................................................................

0.33

................GTTCGAAAAGATGAGAAACTGTCGT..................................................................................................................................................................................................................

0.33

.....................................................................................................................GCAGCTCGAAGAGATTAGGCT.................................................................................................................

0.33

.....................................................................................................................................................................................................TTAGGCTAGAACTGAATCGTC.................................

0.33

..GTGGAGGGGAATGTGTTCG......................................................................................................................................................................................................................................

0.33

AAGTGGAGGGGAATGTGTT........................................................................................................................................................................................................................................

0.33

....................GAAAAGATGAGAAACTGTCG...................................................................................................................................................................................................................

0.33

........................................................................CATTGGTTTTCAGATCAAG................................................................................................................................................................

0.30

0.25

.............................................................................GTTTTCAGATCAAGAGTA............................................................................................................................................................

0.22

0.14

.............................................................................GTTTTCAGATCAAGGTAATG..........................................................................................................................................................

0.14

.........................................................................ATTGGTTTTCAGATCAAGGT..............................................................................................................................................................

0.06

Species	Coordinate	ID	Alignment
droGri2	scaffold_15112:3030097-3030347 -	dgr_360	AAGTGGAGGGGAATGTGTTCGAAAAGATGAGAAACTGTCGTATATATCTCTTAAATTTGACAACAAAACAGAAATACGTTTTCAGATCAAGGATATCTGCAGAAAAGTTGAGCAATTGCAGCTCGAAGAGATTAGGTTTGTATTGGAAACCCGACATGTATTTCAAAAAAATTTGAAAGATTCTTATCACCACATTTGCAGGCTAGAACTGAATCGTCAGAGCGAATTGCAGAATCGTCAGATCGATTTGC
droVir3	scaffold_12875:2924729-2924856 +		GTGTGAAGAGAATAATGTACACAATCTTGGAAAACTGGGCAACTTAACAATCAAGTATGGAAATACGGCGGAAATACATTTTCAAATAATAGACCCTAGCAGCAAGTTGCTGCACTCAAAGATCGAAG---------------------------------------------------------------------------------------------------------------------------
droMoj3	scaffold_6496:22282305-22282429 -		GTGCGAAGA----T--------GCTTTTGCGAAAATTGGAAATTTATCAATTAAATATGGTAATCAAACAGAAATACATTTTGCAATTAAAGATTCAAATGGAATGACGCTGCAAGCGCAGCTCGAAGCGCTGAAGT------------------------------------------------------------------------------------------------------------------

Species

Coordinate

Alignment

droGri2

scaffold_15112:3030097-3030347 -

dgr_360

AAGTGGAGGGGAATGTGTTCGAAAAGATGAGAAACTGTCGTATATATCTCTTAAATTTGACAACAAAACAGAAATACGTTTTCAGATCAAGGATATCTGCAGAAAAGTTGAGCAATTGCAGCTCGAAGAGATTAGGTTTGTATTGGAAACCCGACATGTATTTCAAAAAAATTTGAAAGATTCTTATCACCACATTTGCAGGCTAGAACTGAATCGTCAGAGCGAATTGCAGAATCGTCAGATCGATTTGC

droVir3

scaffold_12875:2924729-2924856 +

GTGTGAAGAGAATAATGTACACAATCTTGGAAAACTGGGCAACTTAACAATCAAGTATGGAAATACGGCGGAAATACATTTTCAAATAATAGACCCTAGCAGCAAGTTGCTGCACTCAAAGATCGAAG---------------------------------------------------------------------------------------------------------------------------

droMoj3

scaffold_6496:22282305-22282429 -

GTGCGAAGA----T--------GCTTTTGCGAAAATTGGAAATTTATCAATTAAATATGGTAATCAAACAGAAATACATTTTGCAATTAAAGATTCAAATGGAATGACGCTGCAAGCGCAGCTCGAAGCGCTGAAGT------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droGri2

AAGTGGAGGGGAATGTGTTCGAAAAGATGAGAAACTGTCGTATATATCTCTTAAATTTGACAACAAAACAGAAATACGTTTTCAGATCAAGGATATCTGCAGAAAAGTTGAGCAATTGCAGCTCGAAGAGATTAGGTTTGTATTGGAAACCCGACATGTATTTCAAAAAAATTTGAAAGATTCTTATCACCACATTTGCAGGCTAGAACTGAATCGTCAGAGCGAATTGCAGAATCGTCAGATCGATTTGC	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
.AGTGGAGGGGAATGTGTTC.......................................................................................................................................................................................................................................	19	3	1.67	5	1	4
.................................................................................................................AATTGCAGCTCGAAGAGATTAG....................................................................................................................	22	3	1.00	3	0	3
.AGTGGAGGGGAATGTGTT........................................................................................................................................................................................................................................	18	3	1.00	3	1	2
.AGTGGAGGGGAATGTGTTCG......................................................................................................................................................................................................................................	20	3	1.00	3	2	1
AAGTGGAGGGGAATGTGTTC.......................................................................................................................................................................................................................................	20	3	1.00	3	1	2
.............................................................................................................................................................................................................GAACTGAATCGTCAGAGCG...........................	19	3	0.67	2	0	2
...............................................................................................................................................................................................................................GAATTGCAGAATCGTCAGATC.......	21	2	0.50	1	1	0
...............................................................................................................................................................................................................................GAATTGCAGAATCGTCAGATCGA.....	23	2	0.50	1	0	1
..........................................................................................................................................................................................................................AGAGCGAATTGCAGAATCGTC............	21	2	0.50	1	1	0
................................................................................................................................................................................................................CTGAATCGTCAGAGCGAATTGCAGAATC...............	28	2	0.50	1	1	0
..............................................................................................................................................................................................................................CGAATTGCAGAATCGTCAGATC.......	22	2	0.50	1	1	0
.......................................................................................................................................................................................................................GTCAGAGCGAATTGCAGAATCGTCAGA.........	27	2	0.50	1	0	1
................................................................................................................................................................................................................CTGAATCGTCAGAGCGAATTGCA....................	23	2	0.50	1	1	0
................................................................................................................................................................................................................................AATTGCAGAATCGTCAGATCGATTTG.	26	2	0.50	1	0	1
.....................................................................................................................GCAGCTCGAAGAGATTAGG...................................................................................................................	19	3	0.33	1	1	0
........................................................................................................AAGTTGAGCAATTGCAGCT................................................................................................................................	19	3	0.33	1	1	0
.AGTGGAGGGGAATGTGTTCGA.....................................................................................................................................................................................................................................	21	3	0.33	1	0	1
.....................AAAAGATGAGAAACTGTCGTATATA.............................................................................................................................................................................................................	25	3	0.33	1	0	1
............................................................................................................................................................................................................AGAACTGAATCGTCAGAGC............................	19	3	0.33	1	1	0
.............................AGAAACTGTCGTATATATCTCTTA......................................................................................................................................................................................................	24	3	0.33	1	0	1
........................AGATGAGAAACTGTCGTATATATCT..........................................................................................................................................................................................................	25	3	0.33	1	0	1
AAGTGGAGGGGAATGTGTTCG......................................................................................................................................................................................................................................	21	3	0.33	1	0	1
.................TTCGAAAAGATGAGAAACT.......................................................................................................................................................................................................................	19	3	0.33	1	1	0
..................................................................................................................................................................................................................GAATCGTCAGAGCGAATT.......................	18	3	0.33	1	1	0
................GTTCGAAAAGATGAGAAACTGTCGT..................................................................................................................................................................................................................	25	3	0.33	1	0	1
..GTGGAGGGGAATGTGTTCG......................................................................................................................................................................................................................................	19	3	0.33	1	0	1
AAGTGGAGGGGAATGTGTT........................................................................................................................................................................................................................................	19	3	0.33	1	0	1
....................GAAAAGATGAGAAACTGTCG...................................................................................................................................................................................................................	20	3	0.33	1	1	0
....................................................................................................................................................................................................................................GCAGAATCGTCAGATCGATT...	20	4	0.25	1	1	0

droVir3

GTGTGAAGAGAATAATGTACACAATCTTGGAAAACTGGGCAACTTAACAATCAAGTATGGAAATACGGCGGAAATACATTTTCAAATAATAGACCCTAGCAGCAAGTTGCTGCACTCAAAGATCGAAG---------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M027 Male-body	M028 Head	M047 Female-body	M061 Embryo	SRR060654 Ovary	SRR060655 Testis	SRR060656 Ovary	SRR060657 Testis	SRR060658 Ovary	SRR060659 Testis	SRR060660 Ovary	SRR060661 Embryo	SRR060662 Embryo	SRR060663 Embryo	SRR060664 Male-body	SRR060665 Female-body	SRR060666 Male-body	SRR060667 Female-body	SRR060668 Male-body	SRR060669 Female-body	SRR060670 Testis	SRR060671 Male-body	SRR060672 Female-body	SRR060673 Ovary	SRR060674 Ovary	SRR060675 Ovary	SRR060676 Ovary	SRR060677 Ovary	SRR060678 Testis	SRR060679 Testis	SRR060680 Testis	SRR060681 Testis	SRR060682 Embryo	SRR060683 Testis	SRR060684 Embryo	SRR060685 Embryo	SRR060686 Embryo	SRR060687 Embryo	SRR060688 Ovary	SRR060689 Testis	SRR1106712 embryo_0-2h	SRR1106713 embryo_0-2h	SRR1106714 embryo_2-4h	SRR1106715 embryo_4-6h	SRR1106716 embryo_4-6h	SRR1106717 embryo_6-8h	SRR1106718 embryo_6-8h	SRR1106719 embryo_8-10h	SRR1106720 embryo_8-10h	SRR1106721 embryo_10-12h	SRR1106722 embryo_10-12h	SRR1106723 embryo_12-14h	SRR1106724 embryo_12-14h	SRR1106725 embryo_14-16h	SRR1106726 embryo_14-16h	SRR1106727 larvae	SRR1106728 larvae	SRR1106729 mixed whole adult body	SRR1106730 embryo_16-30h	V047 Embryo	V053 Head	V116 Male-body	GSM1528803 follicle cells
.....................................................................................ATAATAGACCCTAGCAGCAAGTTGCT............................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droMoj3

GTGCGAAGA----T--------GCTTTTGCGAAAATTGGAAATTTATCAATTAAATATGGTAATCAAACAGAAATACATTTTGCAATTAAAGATTCAAATGGAATGACGCTGCAAGCGCAGCTCGAAGCGCTGAAGT------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
No Reads

dgr_360

scaffold_15112:3030147-3030297 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-25.4	-25.0	-24.9