ID:

dan_85

 Coordinate:

scaffold_13417:1620961-1621037 +

 Confidence:

candidate

 Class:

Canonical miRNA

 Genomic Locale:

intergenic

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-32.5 -31.8 -31.7 -31.7

Flybase annnotation

intergenic

No Repeatable elements found

Sense Strand Reads

CCCCCCCTGAGTTCCTGCCCACATGGATCTAAAGCGTACCGGACAGCCTGGTCCATTGGTGAGTTTGTGGATAGGATTCTGGCCTTCTAGCTGGTCGAGTATGGCACAGAGCACCTTGTCTGGAACCGTCCAGGGAATTTCGAAGGAGATGCGGAAGTGAAGCGAGGGAGTGTGGAG

***********************************....((..........(((.((((((.((((..(((((((.((((((((.....(((.....)))..))).)))))...))))))).)))))).)))))))....))*********************************** 		Read size # Mismatch Hit Count Total Norm Total V105

male body		 V055

head

 V106

head

 M044

female body

 V039

embryo
.......................................................................................................GCACAGAGCACCTTGTCTGGAAC................................................... 23 0 1 35.00 35 31 1 1 2 0
........................................................................................................CGCAGAGCACCTTGTCTGGAA.................................................... 21 1 1 18.00 18 17 0 1 0 0
.......................................................................................................GCGCAGAGCACCTTGTCTGGAA.................................................... 22 1 1 13.00 13 12 1 0 0 0
........................................................................................................CGCAGAGCACCTTGTCTGGAAC................................................... 22 1 1 10.00 10 10 0 0 0 0
.......................................................................................................GCGCAGAGCACCTTGTCTGGAAC................................................... 23 1 1 7.00 7 6 0 1 0 0
............................CTAAAGCGTACCGGACAGCC................................................................................................................................. 20 0 1 4.00 4 4 0 0 0 0
........................................................................................................CGCAGAGCACCTTGTCTGG...................................................... 19 1 1 4.00 4 4 0 0 0 0
.......................................................................................................GCACAGAGCACCTTGTCTGGAA.................................................... 22 0 1 4.00 4 3 1 0 0 0
........................................................................................................CGCAGAGCACCTTGTCTGGA..................................................... 20 1 1 4.00 4 4 0 0 0 0
..................................................................................CCTTCTAGCTGGTCGAGTATG.......................................................................... 21 0 1 3.00 3 3 0 0 0 0
............................CTAAAGCGTACCGGACAGCCT................................................................................................................................ 21 0 1 3.00 3 3 0 0 0 0
.........................................................................................................GCAGAGCACCTTGTCTGGAAC................................................... 21 1 1 2.00 2 2 0 0 0 0
........................................................................................................CGCAGAGCACCTTGTCTGGAATT.................................................. 23 3 1 1.00 1 1 0 0 0 0
............................CTAAAGCGTACCGGACATCCT................................................................................................................................ 21 1 1 1.00 1 0 0 1 0 0
.......................................................................................................GCACAGAGTACCTAGCCTGGAACC.................................................. 24 3 1 1.00 1 0 1 0 0 0
........................................................................................................CGCATAGCACCTTGTCTGGAAC................................................... 22 2 1 1.00 1 1 0 0 0 0
.........................................................................................................GCAGAGCACCTTGTCTGGAACC.................................................. 22 1 1 1.00 1 1 0 0 0 0
...........................TCTAAAGCGTACCGGACAGCC................................................................................................................................. 21 0 1 1.00 1 1 0 0 0 0
.......................................................................................................GCACAGAGCACCTTGTCTGGAACC.................................................. 24 0 1 1.00 1 1 0 0 0 0
............................CTAAAGCGTACCGGACAGT.................................................................................................................................. 19 1 2 1.00 2 2 0 0 0 0
............................................................................................GGTCGAGTATGGCACAGAGC................................................................. 20 0 1 1.00 1 1 0 0 0 0
..................................................................................CCTTCTAGCTCGTCGAGTATG.......................................................................... 21 1 1 1.00 1 1 0 0 0 0
...........................................................................................................................................................GGTGATGCGAGGAAGTGTGGAG 22 3 1 1.00 1 0 1 0 0 0
........................................................................................................CGCAGAGCACCTTGTCTGGG..................................................... 20 2 1 1.00 1 1 0 0 0 0
............................................................................................GGTCGAGTATGGCACAGAGCAC............................................................... 22 0 1 1.00 1 1 0 0 0 0
.......................................................................................................GCGCAGAGCACCTTGTCTGG...................................................... 20 1 1 1.00 1 0 0 0 1 0
........................................................................................................CGCAGAGCACATTGTCTGGAAC................................................... 22 2 1 1.00 1 1 0 0 0 0
........................................................................................................CACAGAGCACCTTGTCTGGAA.................................................... 21 0 1 1.00 1 1 0 0 0 0
............................CTAAAGCGTACCGGACAGC.................................................................................................................................. 19 0 1 1.00 1 1 0 0 0 0
..................................................................................CCTTATAGCTGGCCGAGTATG.......................................................................... 21 2 1 1.00 1 0 1 0 0 0
.......................................................................................................................................................CGGCAGTGAAGCTAGGGAGTT..... 21 3 2 0.50 1 0 1 0 0 0
........................................................................................................GGCAGAGCACCTTGTCTGGAAT................................................... 22 3 2 0.50 1 0 0 1 0 0
........................................................................................................CGCAGAGCACCTTGTCTGTAA.................................................... 21 2 2 0.50 1 1 0 0 0 0
..................................................GTCCATTGGTGATTTTGT............................................................................................................. 18 1 2 0.50 1 0 0 0 0 1
........................................TGACAGCGTGGTTCATTGGT..................................................................................................................... 20 3 3 0.33 1 0 1 0 0 0
.........................GATCTACTGCGTAGCGGAC..................................................................................................................................... 19 3 9 0.33 3 0 0 0 3 0
................................................................TTGTGGATAGGATAGTGG............................................................................................... 18 2 7 0.14 1 0 1 0 0 0
............................CTAAATCGTACCGGTCAAC.................................................................................................................................. 19 3 8 0.13 1 0 0 0 1 0
.....................................................CAGTGGTGAGGTTGTGGAAA........................................................................................................ 20 3 14 0.07 1 0 0 0 1 0
............................................................................................................................................................GTGAAGCGAGATAGTGTGG.. 19 2 20 0.05 1 1 0 0 0 0

Anti-sense strand reads

GGGGGGGACTCAAGGACGGGTGTACCTAGATTTCGCATGGCCTGTCGGACCAGGTAACCACTCAAACACCTATCCTAAGACCGGAAGATCGACCAGCTCATACCGTGTCTCGTGGAACAGACCTTGGCAGGTCCCTTAAAGCTTCCTCTACGCCTTCACTTCGCTCCCTCACACCTC

***********************************....((..........(((.((((((.((((..(((((((.((((((((.....(((.....)))..))).)))))...))))))).)))))).)))))))....))*********************************** 		Read size # Mismatch Hit Count Total Norm Total M044

female body

 V106

head

 V055

head

 M058

embryo

 V105

male body		 V039

embryo
..................................................................CACCTATCCTAAGAGTGGA............................................................................................ 19 2 2 3.00 6 6 0 0 0 0 0
......................................................................................................CCGTGTTTTGTGGTACAGAC....................................................... 20 3 5 1.60 8 0 5 2 0 1 0
.................................................................CCACCTATCCTAAGAGTGGA............................................................................................ 20 3 3 1.00 3 2 0 0 1 0 0
.......................................................................................................CGTGTTTTGTGGTACAGAC....................................................... 19 3 20 0.75 15 10 4 0 1 0 0
......................................................................................................CCGTGTTTTGTGGTACAGA........................................................ 19 3 20 0.50 10 10 0 0 0 0 0
..................................................................CACCTATCCTAAGAGTGG............................................................................................. 18 2 4 0.50 2 1 1 0 0 0 0
......................................GGCCTGTAGGGCCAGGTA......................................................................................................................... 18 2 3 0.33 1 0 0 1 0 0 0
.............................................................TAAGCCACCTATCCTAAGA................................................................................................. 19 3 20 0.30 6 4 0 0 1 0 1
..................................................................CACCTATCCTAAGAGTGGAT........................................................................................... 20 3 6 0.17 1 1 0 0 0 0 0
...................................................................ACCTATCCTAAGAGTGGA............................................................................................ 18 2 6 0.17 1 1 0 0 0 0 0
.....................................TGGCCTATAGGGCCAGGTA......................................................................................................................... 19 3 8 0.13 1 0 0 1 0 0 0
.................................................................CCACCTATCCTAAGAGTGG............................................................................................. 19 3 15 0.07 1 1 0 0 0 0 0
..................................................................................GGAAGTTCGACCAGTTCA............................................................................. 18 2 20 0.05 1 0 0 1 0 0 0

Re-alignment of all predicted orthologs

Species Coordinate ID Type Alignment
droAna3 scaffold_13417:1620911-1621087 + dan_85 candidate CCCCCCCTGAGTTCCTGCCCACATGGATCTAAAG-----CGTACCG-----GACAGCCTGGTCCATTGGTGAGTTTGTGGATAG-GATTCTGGCCTTCTAGCTGGTCGAGTATGGCACAGAGCACCTTGTCTGGAACCGTCCAGGG---AATTTCGAAGGAGATGCGGAAGTGAAGCGAGGGAGT-GTGGA--------------------G
droBip1 scf7180000396805:6932-7095 + CGTCTCCCCAATTTCTGAGAACATGG-------CTAAATCCTTTCA-----GGCAACAGGGCCCCTTGGTGTGTATGTGGACCGCCACTCTGGTTTTCTAGCTCATCAAGTATAGCTCCTAGGACCTTGTCTGGAAGCGTGGAGGACGAAACTACGCAGAAGACGGGGACGTGCAG------------------------------------
droTak1 scf7180000414450:137216-137405 + CACTCACTG--T-------CACATGGATCTAGGT-----GATACCGTTCCACG----ACACTCCACTTGTCGGTCACAAGATCGGGATTCTGCATCTCCAGCAGGTCGAAAACAGAGCACAAGGCTTCTCCCGGAATGACCAATGG---CAACACGAATGAGCACCGTATA-AGATCGAAAAAGTTGATGAATGTCTGTGCCATATGGGGGG

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droAna3
CCCCCCCTGAGTTCCTGCCCACATGGATCTAAAG-----CGTACCG-----GACAGCCTGGTCCATTGGTGAGTTTGTGGATAG-GATTCTGGCCTTCTAGCTGGTCGAGTATGGCACAGAGCACCTTGTCTGGAACCGTCCAGGG---AATTTCGAAGGAGATGCGGAAGTGAAGCGAGGGAGT-GTGGA--------------------GSizeHit CountTotal NormTotalM044

Female-body		M058

Embryo		V039

Embryo		V055

Head		V105

Male-body		V106

Head
..................................................................................................................GCACAGAGCACCTTGTCTGGAAC...........................................................................23135.00352001311
............................CTAAAG-----CGTACCG-----GACAGCC..........................................................................................................................................................2014.004000040
..................................................................................................................GCACAGAGCACCTTGTCTGGAA............................................................................2214.004000130
.............................................................................................CCTTCTAGCTGGTCGAGTATG..................................................................................................2113.003000030
............................CTAAAG-----CGTACCG-----GACAGCCT.........................................................................................................................................................2113.003000030
...........................TCTAAAG-----CGTACCG-----GACAGCC..........................................................................................................................................................2111.001000010
..................................................................................................................GCACAGAGCACCTTGTCTGGAACC..........................................................................2411.001000010
............................CTAAAG-----CGTACCG-----GACAGT...........................................................................................................................................................1921.002000020
.......................................................................................................GGTCGAGTATGGCACAGAGC.........................................................................................2011.001000010
.......................................................................................................GGTCGAGTATGGCACAGAGCAC.......................................................................................2211.001000010
...................................................................................................................CACAGAGCACCTTGTCTGGAA............................................................................2111.001000010
............................CTAAAG-----CGTACCG-----GACAGC...........................................................................................................................................................1911.001000010
droBip1
CGTCTCCCCAATTTCTGAGAACATGG-------CTAAATCCTTTCA-----GGCAACAGGGCCCCTTGGTGTGTATGTGGACCGCCACTCTGGTTTTCTAGCTCATCAAGTATAGCTCCTAGGACCTTGTCTGGAAGCGTGGAGGACGAAACTACGCAGAAGACGGGGACGTGCAG------------------------------------SizeHit CountTotal NormTotal
No Reads
droTak1
CACTCACTG--T-------CACATGGATCTAGGT-----GATACCGTTCCACG----ACACTCCACTTGTCGGTCACAAGATCGGGATTCTGCATCTCCAGCAGGTCGAAAACAGAGCACAAGGCTTCTCCCGGAATGACCAATGG---CAACACGAATGAGCACCGTATA-AGATCGAAAAAGTTGATGAATGTCTGTGCCATATGGGGGGSizeHit CountTotal NormTotal
No Reads

Generated: 10/20/2015 at 06:55 PM