ID:

dan_77

 Coordinate:

scaffold_12903:550718-550778 +

 Confidence:

confident

 Class:

Testes-restricted

 Genomic Locale:

antisense_to_intron

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-27.1 -26.8 -26.5

Flybase annnotation

Antisense to intron [Dana\GF15947-in]

No Repeatable elements found

Sense Strand Reads

ACTATTCATTATAATAATATCTTATGAATATCTTATATAATATCACTTTTGAGAGGTACGGAATGCTTGTATGTGACCTTGAACTTCATGGAAGCATTTGGCGTACATCTAAAAAGTTATATTTATTTCCCAATATCCCTCAGAACTTTTCAAACCGCCCT

***********************************...(((((.((((((.(((.(((((((((((((.((((.............)))).))))))))..))))).))).)))))).)))))..************************************ 		Read size # Mismatch Hit Count Total Norm Total V105

male body		 M044

female body

 V106

head

 V055

head

 M058

embryo
..................................................GAGAGGTACGGAATGCTTGTATG........................................................................................ 23 0 1 40.00 40 29 1 7 3 0
........................................................................................TGGAAGCATTTGGCGTACATCT................................................... 22 0 1 31.00 31 11 15 0 4 1
..................................................GAGAGGTACGGAATGCTTGTAT......................................................................................... 22 0 1 25.00 25 17 1 7 0 0
...................................................AGAGGTACGGAATGCTTGTATG........................................................................................ 22 0 1 20.00 20 13 1 3 3 0
........................................................................................TGGAAGCATTTGGCGTACATC.................................................... 21 0 1 11.00 11 5 2 0 1 3
........................................................................................TGGAAGCATTTGGCGTACATCTA.................................................. 23 0 1 7.00 7 5 2 0 0 0
........................................................................................TGGAAGCATTTGGCGTACATCTAA................................................. 24 0 1 6.00 6 4 2 0 0 0
...................................................AGAGGTACGGAATGCTTGTAT......................................................................................... 21 0 1 5.00 5 1 0 3 1 0
....................................................GAGGTACGGAATGCTTGTATG........................................................................................ 21 0 1 5.00 5 5 0 0 0 0
..................................................GAGAGGTACGGAATGCTTGT........................................................................................... 20 0 1 5.00 5 2 0 2 1 0
........................................................................................TGGAAGCATTTGGCGTACATCTAAA................................................ 25 0 1 3.00 3 0 0 1 0 2
..................................................GAGAGGTACGGAATGCTTGTA.......................................................................................... 21 0 1 3.00 3 2 0 0 1 0
........................................................................................TGGAAGCATTTGGCGTACATCTT.................................................. 23 1 1 2.00 2 0 1 0 1 0
...................................................AGAGGTACGGAATGCTTGT........................................................................................... 19 0 1 1.00 1 0 1 0 0 0
....................................................GAGGCACGGAATGCTTGTATG........................................................................................ 21 1 1 1.00 1 1 0 0 0 0
........................................................................................TGGAAGCATTTGGCGTACATCTAT................................................. 24 1 1 1.00 1 1 0 0 0 0
........................................................................................TGGAAGCATTTGGCGTACATCTAC................................................. 24 1 1 1.00 1 1 0 0 0 0
........................................................................................TGGAAGCATTTGACGTACATCT................................................... 22 1 1 1.00 1 1 0 0 0 0
.........................................................................................TGAAGCATTTGGCGTACATCT................................................... 21 1 1 1.00 1 0 1 0 0 0
....................................................GAGTTACGGAATGCTTGTATG........................................................................................ 21 1 1 1.00 1 1 0 0 0 0
..................................................GAGAGGTACGGAATGCTTGTCTG........................................................................................ 23 1 1 1.00 1 1 0 0 0 0
..................................................GAGAGGTACGGAATGCTTGTATGT....................................................................................... 24 0 1 1.00 1 0 0 1 0 0
.........................................................................................GGAAGCATGTGGCGTACATCT................................................... 21 1 1 1.00 1 0 1 0 0 0
..................................................GAGAGGTACGGAATGTTTGTATG........................................................................................ 23 1 1 1.00 1 1 0 0 0 0
....................................................GAGGTACGGAATGCTTGTATGTGACC................................................................................... 26 0 1 1.00 1 1 0 0 0 0
..................................................GAGAGGTACGGAATGCTTGTATA........................................................................................ 23 1 1 1.00 1 1 0 0 0 0
..................................................GAGAGGTAAGCAATGCTTGTAT......................................................................................... 22 2 1 1.00 1 1 0 0 0 0
........................................................................................CGTAAGCATTTGGCGTACATCT................................................... 22 2 1 1.00 1 1 0 0 0 0
........................................................................................TTGAAGCATTTGGCGTACATCC................................................... 22 2 1 1.00 1 0 0 0 0 1
........................................................................................TGGAAGCATTTGGCGTACATCTAAAA............................................... 26 0 1 1.00 1 1 0 0 0 0
..................................................GAGAGGTATGGAATGCTTGTAT......................................................................................... 22 1 1 1.00 1 1 0 0 0 0
..................................................GAGAGGTACGGAATGCTTGTTTG........................................................................................ 23 1 1 1.00 1 0 0 1 0 0
........................................................................................TGGAAGCATTTGGCGTACATAT................................................... 22 1 1 1.00 1 1 0 0 0 0
.........................................................................................GGAAGCATTTGGCGTACATCT................................................... 21 0 1 1.00 1 0 1 0 0 0
...........................................................GGAGTGATCGTATGTGACCTTG................................................................................ 22 3 1 1.00 1 0 0 0 1 0
........................................................................................TGGAAGCATTTGGCGTACACC.................................................... 21 1 1 1.00 1 0 0 0 1 0
..................................................GAGAGGTACGGAATGCTTGTG.......................................................................................... 21 1 1 1.00 1 1 0 0 0 0
..................................................GAGAGGTACGGAATGCTCGTATG........................................................................................ 23 1 1 1.00 1 0 0 1 0 0
..................................................GAGAGGTATGAAATGCTTGTCTG........................................................................................ 23 3 1 1.00 1 1 0 0 0 0
........................................................................................TGGAAGCATTTGGCGTACAT..................................................... 20 0 1 1.00 1 0 0 0 1 0
..................................................GAGAGGTACGCAATGCTTGTATG........................................................................................ 23 1 1 1.00 1 1 0 0 0 0
........................................................................................TGGAAGCATTTTGCGTACATC.................................................... 21 1 1 1.00 1 1 0 0 0 0
..........................................TCGCTTGTGAGAGGTACGG.................................................................................................... 19 2 12 0.33 4 2 0 2 0 0
...........................................CGCTTGTGAGAGGTACGG.................................................................................................... 18 2 12 0.08 1 0 1 0 0 0
.............................................CTTGTGAGAGGTACGGGA.................................................................................................. 18 2 12 0.08 1 0 1 0 0 0
..........................................TCGCTTGTGAGAGGTACG..................................................................................................... 18 2 14 0.07 1 1 0 0 0 0

Anti-sense strand reads

TGATAAGTAATATTATTATAGAATACTTATAGAATATATTATAGTGAAAACTCTCCATGCCTTACGAACATACACTGGAACTTGAAGTACCTTCGTAAACCGCATGTAGATTTTTCAATATAAATAAAGGGTTATAGGGAGTCTTGAAAAGTTTGGCGGGA

************************************...(((((.((((((.(((.(((((((((((((.((((.............)))).))))))))..))))).))).)))))).)))))..*********************************** 		Read size # Mismatch Hit Count Total Norm Total M058

embryo

 V055

head

 M044

female body

 V039

embryo
...............................................................................................................................AGGGTTATAGGGAGTCTTGAAA............ 22 0 1 1.00 1 1 0 0 0
...............................................................................................................................AGGGTTATAGGGAGTCTTG............... 19 0 1 1.00 1 1 0 0 0
........................................................................................................................TAAATATAGGGTTATCGAGAGT................... 22 3 1 1.00 1 0 1 0 0
...........................................................................TGGAACTTGTAGTGCCTTCG.................................................................. 20 2 2 0.50 1 0 0 1 0
..................................................CCCTCCATTCCTGACGAACA........................................................................................... 20 3 6 0.17 1 0 1 0 0
..................................................................................................ACCGCATGTAGATGATACA............................................ 19 3 10 0.10 1 0 0 0 1

Re-alignment of all predicted orthologs

Species Coordinate ID Alignment
droAna3 scaffold_12903:550668-550828 + dan_77 ACTATTCATTATAATAATATCTTATGAATATCTTATATAATATCACTTTTGAGAGGTACGGAATGCTTGTATGTGACCTTGAACTTCATGGAAGCATTTGGCGTACATCTAAAAAGTTATATTTATT-----------------------------------TCCCAATATCCCTCA---------------------------------------------GAACTTTTCAAACCGCCCT
droBip1 scf7180000395912:19320-19551 + AAATGTGCGAATAAAATAATTATGTCAATATGTATAGTGTTTTCTCAT-------ATCCAAAGTGTTTTAATGTATTTTTTAAATTCATAACTTCATTT--TGTACATTGGAAAAATTCTATATATTTAAACCTATGTTAAAGAGTTAAAGTTGAGTTGAGTTCCCACATTCATTCAATGTGATATTTCCAAATGGGGTATTAACATCTTGGCATTGGTATATAACTACTCTAGCTGCCCT
droRho1 scf7180000778091:424734-424760 + AT-------------------------------------------------------------------------------------------------------------------------------------------------------------------------CCATCC---------------------------------------------CAACTTTTCAAACCACCCT

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droAna3
ACTATTCATTATAATAATATCTTATGAATATCTTATATAATATCACTTTTGAGAGGTACGGAATGCTTGTATGTGACCTTGAACTTCATGGAAGCATTTGGCGTACATCTAAAAAGTTATATTTATT-----------------------------------TCCCAATATCCCTCA---------------------------------------------GAACTTTTCAAACCGCCCTSizeHit CountTotal NormTotalM044

Female-body		M058

Embryo		V039

Embryo		V055

Head		V105

Male-body		V106

Head
..................................................GAGAGGTACGGAATGCTTGTATG........................................................................................................................................................................23140.00401003297
........................................................................................TGGAAGCATTTGGCGTACATCT...................................................................................................................................22131.003115104110
..................................................GAGAGGTACGGAATGCTTGTAT.........................................................................................................................................................................22125.00251000177
...................................................AGAGGTACGGAATGCTTGTATG........................................................................................................................................................................22120.00201003133
........................................................................................TGGAAGCATTTGGCGTACATC....................................................................................................................................21111.0011230150
........................................................................................TGGAAGCATTTGGCGTACATCTA..................................................................................................................................2317.007200050
........................................................................................TGGAAGCATTTGGCGTACATCTAA.................................................................................................................................2416.006200040
...................................................AGAGGTACGGAATGCTTGTAT.........................................................................................................................................................................2115.005000113
....................................................GAGGTACGGAATGCTTGTATG........................................................................................................................................................................2115.005000050
..................................................GAGAGGTACGGAATGCTTGT...........................................................................................................................................................................2015.005000122
........................................................................................TGGAAGCATTTGGCGTACATCTAAA................................................................................................................................2513.003020001
..................................................GAGAGGTACGGAATGCTTGTA..........................................................................................................................................................................2113.003000120
........................................................................................TGGAAGCATTTGGCGTACATCTT..................................................................................................................................2312.002100100
...................................................AGAGGTACGGAATGCTTGT...........................................................................................................................................................................1911.001100000
........................................................................................TGGAAGCATTTGGCGTACATCTAT.................................................................................................................................2411.001000010
........................................................................................TGGAAGCATTTGGCGTACATCTAC.................................................................................................................................2411.001000010
..................................................GAGAGGTACGGAATGCTTGTATGT.......................................................................................................................................................................2411.001000001
....................................................GAGGTACGGAATGCTTGTATGTGACC...................................................................................................................................................................2611.001000010
..................................................GAGAGGTACGGAATGCTTGTATA........................................................................................................................................................................2311.001000010
........................................................................................TGGAAGCATTTGGCGTACATCTAAAA...............................................................................................................................2611.001000010
.........................................................................................GGAAGCATTTGGCGTACATCT...................................................................................................................................2111.001100000
..................................................GAGAGGTACGGAATGCTTGTG..........................................................................................................................................................................2111.001000010
........................................................................................TGGAAGCATTTGGCGTACAT.....................................................................................................................................2011.001000100
droBip1
AAATGTGCGAATAAAATAATTATGTCAATATGTATAGTGTTTTCTCAT-------ATCCAAAGTGTTTTAATGTATTTTTTAAATTCATAACTTCATTT--TGTACATTGGAAAAATTCTATATATTTAAACCTATGTTAAAGAGTTAAAGTTGAGTTGAGTTCCCACATTCATTCAATGTGATATTTCCAAATGGGGTATTAACATCTTGGCATTGGTATATAACTACTCTAGCTGCCCTSizeHit CountTotal NormTotal
No Reads
droRho1
AT-------------------------------------------------------------------------------------------------------------------------------------------------------------------------CCATCC---------------------------------------------CAACTTTTCAAACCACCCTSizeHit CountTotal NormTotal
No Reads

Generated: 09/08/2015 at 08:01 PM