ID:

dan_67

 Coordinate:

scaffold_13117:3425021-3425078 +

 Confidence:

confident

 Class:

Canonical miRNA

 Genomic Locale:

antisense_to_intron

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-25.6 -25.4 -25.4

Flybase annnotation

Antisense to intron [Dana\GF20896-in]

No Repeatable elements found

Sense Strand Reads

GTGAAATAATTGTTAAATTAATTGGAGTTAAAAATACTGTAATTATTGGCCTACGACATGTTGATTTAGTTTATTTTTTAAGCCAAGTTAAATAAACAAGTCGTATGCCAAAAATTATGCAAGCCACAAGATCGATTCCATAAAAAAAAATATTTTTA

***********************************...((((((.(((((.((((((.(((((((((.(((((.....))))).)))))).....))).)))))).))))).)))))).....*********************************** 		Read size # Mismatch Hit Count Total Norm Total V105

male body		 M058

embryo

 M044

female body

 V055

head

 V106

head

 V039

embryo
......................................................................................GTTAAATAAACAAGTCGTATGC.................................................. 22 0 1 104.00 104 47 36 3 6 7 5
......................................................................................GTTAAATAAACAAGTCGTATG................................................... 21 0 1 97.00 97 89 2 2 3 1 0
..................................................CTACGACATGTTGATTTAGTTT...................................................................................... 22 0 1 33.00 33 9 4 14 2 3 1
......................................................................................GGTAAATAAACAAGTCGTATGC.................................................. 22 1 1 10.00 10 1 9 0 0 0 0
......................................................................................GTTAAATAAACAAGTCGTATGCT................................................. 23 1 1 7.00 7 7 0 0 0 0 0
.....................................................................................AGTTAAATAAACAAGTCGTATG................................................... 22 0 1 4.00 4 3 0 0 0 1 0
......................................................................................GTTAAATAAACAAGTAGTATGC.................................................. 22 1 1 3.00 3 1 0 0 2 0 0
..................................................CTACGACATGTTGATTTAGT........................................................................................ 20 0 1 3.00 3 0 0 2 0 1 0
......................................................................................GTTAAATAAACAAGTCGTATGCA................................................. 23 1 1 3.00 3 0 2 0 0 0 1
......................................................................................GTTAAATAAACAAGTCGTATTC.................................................. 22 1 1 3.00 3 2 0 0 0 0 1
.......................................................................................TTAAATAAACAAGTCGTATGC.................................................. 21 0 1 2.00 2 1 1 0 0 0 0
...................................................TACGACATGTTGATTTAGTTT...................................................................................... 21 0 1 2.00 2 1 0 1 0 0 0
......................................................................................GGTTAATAAACAAGTCGTATGC.................................................. 22 2 1 2.00 2 0 2 0 0 0 0
......................................................................................GTTAAATAAACAAGTCGTAT.................................................... 20 0 1 2.00 2 2 0 0 0 0 0
......................................................................................GTTAAATAAACAAGTCGTATGT.................................................. 22 1 1 2.00 2 2 0 0 0 0 0
......................................................................................GTTAGATAAACAAGTCGTATG................................................... 21 1 1 1.00 1 1 0 0 0 0 0
..................................................CTACGACAGGTTGATTTAGTTT...................................................................................... 22 1 1 1.00 1 0 0 1 0 0 0
......................................................................................GTTTAATAAACAAGTCATATGC.................................................. 22 2 1 1.00 1 0 1 0 0 0 0
......................................................................................GTTAAATAAAAAAGTCGTATGC.................................................. 22 1 1 1.00 1 0 0 0 1 0 0
.....................................................................................AGTTAAATAAACAAGTCGTATGT.................................................. 23 1 1 1.00 1 0 0 1 0 0 0
......................................................................................GTTAAATAAACAAGTCGCATGC.................................................. 22 1 1 1.00 1 0 0 0 1 0 0
......................................................................................GTTAAATAAACAAGTCGAATG................................................... 21 1 1 1.00 1 1 0 0 0 0 0
.......................................................................................TTAAATAAACAAGTCGTATGCCT................................................ 23 1 1 1.00 1 1 0 0 0 0 0
........................................................................................TAATTAAACAAGTCGTATGC.................................................. 20 1 1 1.00 1 0 1 0 0 0 0
......................................................................................GTTAAATAAACTAGTCGTATGC.................................................. 22 1 1 1.00 1 1 0 0 0 0 0
......................................................................................GCTAAATAAACAAGTAGTATGC.................................................. 22 2 1 1.00 1 0 0 0 1 0 0
......................................................................................GTTAAATAAACACGTCGTAT.................................................... 20 1 1 1.00 1 1 0 0 0 0 0
..................................................CTACGACATGTTGATTTAGTTTT..................................................................................... 23 1 1 1.00 1 1 0 0 0 0 0
......................................................................................GTTAAATAAACAAGTGGTATGC.................................................. 22 1 1 1.00 1 1 0 0 0 0 0
......................................................................................GGTAAATAAAAAAGTCGTATGCA................................................. 23 3 1 1.00 1 0 1 0 0 0 0
.......................................................................................TTAAATAAACAAGTCGTGTG................................................... 20 1 1 1.00 1 1 0 0 0 0 0
......................................................................................GTTAAATAAACAAGTCGTA..................................................... 19 0 1 1.00 1 0 1 0 0 0 0
......................................................................................GTTAAATAAACAAGTCGAATGC.................................................. 22 1 1 1.00 1 1 0 0 0 0 0
......................................................................................GTTAAATAAACAAGTCGTATGCTA................................................ 24 1 1 1.00 1 0 1 0 0 0 0
.....................................................................................ATTTAAATAAACAAGTCGTATG................................................... 22 1 1 1.00 1 0 1 0 0 0 0
......................................................................................GTTAAATAAACAGGTCGTATGC.................................................. 22 1 1 1.00 1 0 0 0 0 1 0
.......................................................................................TTAAATAAACAAGTCGTATGCC................................................. 22 0 1 1.00 1 1 0 0 0 0 0
.......................................................................................TGAAATAAACAAGTCGTATGC.................................................. 21 1 1 1.00 1 0 1 0 0 0 0
......................................................................................GTTAAATAAACAAGTCGTGTG................................................... 21 1 1 1.00 1 0 1 0 0 0 0
......................................................................................GTTAAATAAACAAGTCGTATGCAA................................................ 24 1 1 1.00 1 0 0 0 0 0 1
.......................................................................................TGAAATAAACAAGTCGTATGCC................................................. 22 1 1 1.00 1 0 1 0 0 0 0
......................................................................................GTTAAATAAACAAGTCTTATGT.................................................. 22 2 1 1.00 1 1 0 0 0 0 0
......................................................................................GTTAAATAAACAAGTCCTATG................................................... 21 1 1 1.00 1 1 0 0 0 0 0
.......................................................................................TTTAATAAACAAGCCGTATGC.................................................. 21 2 1 1.00 1 0 0 0 0 0 1
..................................................CGACGACATGTTGATTTAGTTTAA.................................................................................... 24 2 1 1.00 1 0 1 0 0 0 0
......................................................................................GTTAAATAAACAATTCGTATGC.................................................. 22 1 1 1.00 1 1 0 0 0 0 0
......................................................................................GTTAAATAGACAAGTCGTATGC.................................................. 22 1 1 1.00 1 0 0 1 0 0 0
......................................................................................GGCAAATAAACAAGTCGTATGC.................................................. 22 2 1 1.00 1 0 1 0 0 0 0
.................................................GCTACGACGTGTTGATTTAGTTT...................................................................................... 23 2 1 1.00 1 0 0 1 0 0 0
......................................................................................GTTAAATAAACGAGTCGTATG................................................... 21 1 1 1.00 1 1 0 0 0 0 0
..................................................CTACGACATGTTGATTTAGTT....................................................................................... 21 0 1 1.00 1 0 0 1 0 0 0
......................................................................................GATAAATAAACAAGTCGTATGC.................................................. 22 1 1 1.00 1 1 0 0 0 0 0
......................................................................................GTTAAATAAACAAGTCGTATT................................................... 21 1 1 1.00 1 1 0 0 0 0 0
......................................................................................GTCAAATAAACAAGTCGTATGCA................................................. 23 2 1 1.00 1 0 1 0 0 0 0
......................................................................................GTTAAACAAACAAGTCGTATGC.................................................. 22 1 1 1.00 1 0 1 0 0 0 0
......................................................................................GGTAAATAAACAATTCGTATGC.................................................. 22 2 2 0.50 1 0 1 0 0 0 0
......................................................................................GTTAAATAAACACGCCATATGC.................................................. 22 3 2 0.50 1 0 0 0 1 0 0
......................................................................................GATAAATAAACAAGACGGATGC.................................................. 22 3 2 0.50 1 0 0 0 1 0 0
..................TAATTGGAGATAAAAAGA.......................................................................................................................... 18 2 20 0.05 1 0 1 0 0 0 0
..................................................CAACGACATGTGGAATTAGTTT...................................................................................... 22 3 20 0.05 1 0 0 0 1 0 0
.......................................................................................................................................TTCCATACAAAAAAACATT.... 19 2 20 0.05 1 0 0 0 0 0 1

Anti-sense strand reads

CACTTTATTAACAATTTAATTAACCTCAATTTTTATGACATTAATAACCGGATGCTGTACAACTAAATCAAATAAAAAATTCGGTTCAATTTATTTGTTCAGCATACGGTTTTTAATACGTTCGGTGTTCTAGCTAAGGTATTTTTTTTTATAAAAAT

***********************************...((((((.(((((.((((((.(((((((((.(((((.....))))).)))))).....))).)))))).))))).)))))).....*********************************** 		Read size # Mismatch Hit Count Total Norm Total M044

female body

 M058

embryo
.................AATTAACCTCAATTTTGA........................................................................................................................... 18 1 1 1.00 1 1 0
...................................................AGGCGGTATAACTAAATCAAA...................................................................................... 21 3 8 0.13 1 0 1
..........................................................ACAACCTAATCAAATAAAAAA............................................................................... 21 2 8 0.13 1 0 1
................GAATTAACCTCAATTTTGA........................................................................................................................... 19 2 8 0.13 1 1 0
.............ACCTAATTAACCTCAATTTTG............................................................................................................................ 21 3 11 0.09 1 1 0
...............CGAATTAACCTCAATTTT............................................................................................................................. 18 2 20 0.05 1 0 1

Re-alignment of all predicted orthologs

Species Coordinate ID Type Alignment
droAna3 scaffold_13117:3424971-3425128 + dan_67 confident GTGAAATAATTGTTAAATTAA-TTGG-----------------------------------AGTTAAAAATA---------CTGTAATTATTGGCCTACGACATGTTGA-TTTAGTTTATTTTTTAAGCCAAGTTAAATAAACA--AGTCGTATGCCAAAAATTATGCAAGCCAC----------------------------AAGATCGATTC-CA--TA--------AAAAAAAATATT-----TTT-A
droBip1 scf7180000395895:128435-128590 - GAAATACTATT--------AA-TTGGAATCTTA----------------------------AGTCTAAAATA---------TTGCTGTGGTTGGCCTACGACATGTTGA-TTTAGTTTATGTCTGGAA-CAAATTAAATAAATA--AGTCGTTTGCCAAAAATTATGCAAA--------------------------------AACAACGATTT-AA--AA--------AAA-TAAATAATAAAAATAA-A
droEle1 scf7180000491023:198326-198491 + TGCAA-TAAATATATAATTTA-AAAG---------ATTCATTAAAGA-------------G--------------------------ATACAAGTCTTCGCTAAAAT---ATCAGTTTTTTTATTAACACAAA---------TATT------AGTCCAATAATGAAAT--GTCATACACCTTT--TAACTCGTTATACAATTTCAAACCGATTGACATTCA--------AACCAGAATATT-----TTTAT
droRho1 scf7180000779985:163806-163966 - ATAGG-TTTTTACTTACTTCC-TTAT------AAGATTAATATTGTATTTATATGTAACACCTACAAACATACAAAGAATT--------------TTTCAATATATTTTTTTCAGACTA---------------------CACC--AGACATAAGCCACA----------------AACATATGTAAATGGC-----------A---TTTGTTC-TT--TGTTATAAAATAATTTAA-----------T-A
droYak3 X:6797531-6797649 + ACGAAGGAATT--------AAATCG-----------------------------------GATACGATCAAACTGGAAATTTAGATTTT---------------------------------------TCAAGCAAAAGAAACATTATTTTTAATCTAAAAATAAACTTAGCCAGGAATCTTT--AAATTG----------------------------TA--------TAAATAAA-----------T-C

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droAna3
GTGAAATAATTGTTAAATTAA-TTGG-----------------------------------AGTTAAAAATA---------CTGTAATTATTGGCCTACGACATGTTGA-TTTAGTTTATTTTTTAAGCCAAGTTAAATAAACA--AGTCGTATGCCAAAAATTATGCAAGCCAC----------------------------AAGATCGATTC-CA--TA--------AAAAAAAATATT-----TTT-ASizeHit CountTotal NormTotalM044

Female-body		M058

Embryo		V039

Embryo		V055

Head		V105

Male-body		V106

Head
....................................................................................................................................GTTAAATAAACA--AGTCGTATGC...............................................................................................221104.0010433656477
....................................................................................................................................GTTAAATAAACA--AGTCGTATG................................................................................................21197.00972203891
...............................................................................................CTACGACATGTTGA-TTTAGTTT.....................................................................................................................................22133.00331441293
....................................................................................................................................GTTAAATAAACA--AGTCGTATGCT..............................................................................................2317.007000070
...................................................................................................................................AGTTAAATAAACA--AGTCGTATG................................................................................................2214.004000031
...............................................................................................CTACGACATGTTGA-TTTAGT.......................................................................................................................................2013.003200001
....................................................................................................................................GTTAAATAAACA--AGTCGTATGCA..............................................................................................2313.003021000
.....................................................................................................................................TTAAATAAACA--AGTCGTATGC...............................................................................................2112.002010010
................................................................................................TACGACATGTTGA-TTTAGTTT.....................................................................................................................................2112.002100010
....................................................................................................................................GTTAAATAAACA--AGTCGTAT.................................................................................................2012.002000020
....................................................................................................................................GTTAAATAAACA--AGTCGTATGT...............................................................................................2212.002000020
...................................................................................................................................AGTTAAATAAACA--AGTCGTATGT...............................................................................................2311.001100000
.....................................................................................................................................TTAAATAAACA--AGTCGTATGCCT.............................................................................................2311.001000010
...............................................................................................CTACGACATGTTGA-TTTAGTTTT....................................................................................................................................2311.001000010
....................................................................................................................................GTTAAATAAACA--AGTCGTA..................................................................................................1911.001010000
.....................................................................................................................................TTAAATAAACA--AGTCGTATGCC..............................................................................................2211.001000010
...............................................................................................CTACGACATGTTGA-TTTAGTT......................................................................................................................................2111.001100000
....................................................................................................................................GTTAAATAAACA--AGTCGTATT................................................................................................2111.001000010
droBip1
GAAATACTATT--------AA-TTGGAATCTTA----------------------------AGTCTAAAATA---------TTGCTGTGGTTGGCCTACGACATGTTGA-TTTAGTTTATGTCTGGAA-CAAATTAAATAAATA--AGTCGTTTGCCAAAAATTATGCAAA--------------------------------AACAACGATTT-AA--AA--------AAA-TAAATAATAAAAATAA-ASizeHit CountTotal NormTotal
No Reads
droEle1
TGCAA-TAAATATATAATTTA-AAAG---------ATTCATTAAAGA-------------G--------------------------ATACAAGTCTTCGCTAAAAT---ATCAGTTTTTTTATTAACACAAA---------TATT------AGTCCAATAATGAAAT--GTCATACACCTTT--TAACTCGTTATACAATTTCAAACCGATTGACATTCA--------AACCAGAATATT-----TTTATSizeHit CountTotal NormTotal
No Reads
droRho1
ATAGG-TTTTTACTTACTTCC-TTAT------AAGATTAATATTGTATTTATATGTAACACCTACAAACATACAAAGAATT--------------TTTCAATATATTTTTTTCAGACTA---------------------CACC--AGACATAAGCCACA----------------AACATATGTAAATGGC-----------A---TTTGTTC-TT--TGTTATAAAATAATTTAA-----------T-ASizeHit CountTotal NormTotal
No Reads
droYak3
ACGAAGGAATT--------AAATCG-----------------------------------GATACGATCAAACTGGAAATTTAGATTTT---------------------------------------TCAAGCAAAAGAAACATTATTTTTAATCTAAAAATAAACTTAGCCAGGAATCTTT--AAATTG----------------------------TA--------TAAATAAA-----------T-CSizeHit CountTotal NormTotalM026

Head		M043

Female-body		M056

Embryo		V046

Embryo		V052

Head		V058

Head		V120

Male-body		SRR1275488

Male larvae		SRR1275486

Male prepupae		SRR1275484

Male prepupae		GSM1528802

follicle cells
No Reads

Generated: 10/20/2015 at 06:47 PM