ID:

dan_52

 Coordinate:

scaffold_12903:590516-590575 +

 Confidence:

confident

 Class:

Canonical miRNA

 Genomic Locale:

intergenic

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-21.4 -21.3 -21.0

Flybase annnotation

intergenic

No Repeatable elements found

Sense Strand Reads

TAAAATGAAAATATATCGAATGTGCAAGAAACTCAAAATAAAAGTTGATTCCAAGTCCTTTTTAGAGTCCTTTTTGAAATGAAGAAAGACTGTTTGAAGTGCCTGGGAATTAATTTTGATATTTAAGCAACAGTTTTGAAAGTGAAGAATGAATAAGTAC

***********************************.....((((((((((((..((.((((....((((.(((((......))))).))))....)))).))...)))))))))))).......************************************ 		Read size # Mismatch Hit Count Total Norm Total V106

head

 V105

male body		 M044

female body

 V055

head

 M058

embryo

 V039

embryo
......................................................................................AGACTGTTTGAAGTGCCTGGGAA................................................... 23 0 1 160.00 160 94 29 28 7 1 1
......................................................................................AGACTGTTTGAAGTGCCTGGGA.................................................... 22 0 1 152.00 152 89 26 21 13 2 1
..................................................CCAAGTCCTTTTTAGAGTCCT......................................................................................... 21 0 1 45.00 45 6 23 13 2 1 0
.....................................................................................AAGACTGTTTGAAGTGCCTGGGA.................................................... 23 0 1 23.00 23 10 10 2 1 0 0
.......................................................................................GACTGTTTGAAGTGCCTGGGAA................................................... 22 0 1 16.00 16 12 3 1 0 0 0
......................................................................................AGACTGTTTGAAGTGCCTGGGAT................................................... 23 1 1 13.00 13 6 3 1 3 0 0
......................................................................................AGACTGTTTGAAGTGCCTGGGAAT.................................................. 24 0 1 9.00 9 6 2 0 1 0 0
.......................................................................................GACTGTTTGAAGTGCCTGGGA.................................................... 21 0 1 6.00 6 4 0 2 0 0 0
......................................................................................AGACTGTTTGAAGTGCCTGGGAAA.................................................. 24 1 1 5.00 5 5 0 0 0 0 0
..................................................CCAAGTCCTTTTTAGAGTCC.......................................................................................... 20 0 1 4.00 4 0 1 2 1 0 0
.......................................................................................GACTGTTTGAAGTGCCTGGGAAT.................................................. 23 0 1 3.00 3 2 1 0 0 0 0
......................................................................................AGACTGTTTGAAGTGCCTGGGT.................................................... 22 1 1 3.00 3 2 0 0 1 0 0
......................................................................................AGACTGTTTGAAGTGCCTGGG..................................................... 21 0 1 3.00 3 3 0 0 0 0 0
..................................................CCAAGTCCTTTTTAGAGTCCTT........................................................................................ 22 0 1 3.00 3 0 1 2 0 0 0
......................................................................................AGACTGTTTGAAGTGCCTGGCA.................................................... 22 1 1 2.00 2 1 1 0 0 0 0
..................................................CCAAGTCCTTTTTAGAGTC........................................................................................... 19 0 1 2.00 2 1 0 1 0 0 0
.....................................................................................AAGACTGTTTGAAGTGCCTGG...................................................... 21 0 1 2.00 2 0 1 1 0 0 0
........................................................................................ACTGTTTGAAGTGCCTGGGAA................................................... 21 0 1 2.00 2 2 0 0 0 0 0
..................................................CCAAGTCCGTTTTAGAGTCCT......................................................................................... 21 1 1 2.00 2 0 0 2 0 0 0
......................................................................................AGACTGTTTGAAGTGCCTGGAAA................................................... 23 1 1 2.00 2 1 1 0 0 0 0
......................................................................................AGACTGTTTGAAGAGCCTGGGAA................................................... 23 1 1 2.00 2 1 1 0 0 0 0
......................................................................................AGACTGTTTGAAGTGCCTGGGAAC.................................................. 24 1 1 2.00 2 2 0 0 0 0 0
......................................................................................AGACTGTTTGAAGTGCCTTGGAA................................................... 23 1 1 2.00 2 2 0 0 0 0 0
................................................TTCCAAGTCCTTTTTAGAGTCC.......................................................................................... 22 0 1 1.00 1 0 0 1 0 0 0
.......................................................................................GACTGTTTGAAGTGCCTGGGATT.................................................. 23 1 1 1.00 1 0 0 0 1 0 0
......................................................................................AGACTGTTTGAAGTGCCTGGAA.................................................... 22 1 1 1.00 1 1 0 0 0 0 0
......................................................................................AGACTGTTTGAAGCGCCTGGGA.................................................... 22 1 1 1.00 1 1 0 0 0 0 0
......................................................................................AGACTGTTTGAAGTGCCTGGGATC.................................................. 24 2 1 1.00 1 1 0 0 0 0 0
......................................................................................AGAATGTTTGAAGTGCCTGGGATT.................................................. 24 2 1 1.00 1 0 1 0 0 0 0
.....................................................................................AAGACTGTTTGAAGTGCCTGGGATTT................................................. 26 1 1 1.00 1 1 0 0 0 0 0
...................................................................................GAAAGACTGTTTGAAGTGCCTG....................................................... 22 0 1 1.00 1 0 0 1 0 0 0
.....................................................................................AAGACTGTTTGAAGTGCCTG....................................................... 20 0 1 1.00 1 0 0 1 0 0 0
......................................................................................ACACTGTTTGAAGTGCCTGGGA.................................................... 22 1 1 1.00 1 1 0 0 0 0 0
..................................................CCAAGTCCTTTTTAGACTCCT......................................................................................... 21 1 1 1.00 1 0 1 0 0 0 0
......................................................................................AAACTGTTTGAAGTGCCTGGGA.................................................... 22 1 1 1.00 1 1 0 0 0 0 0
........................................................................................ACTGTTTGAAGTGCCTGGGG.................................................... 20 1 1 1.00 1 1 0 0 0 0 0
......................................................................................AGACTGTTTTAAGTGCCTGGT..................................................... 21 2 1 1.00 1 0 1 0 0 0 0
......................................................................................AGACTGTTTCAAGTGCCTGGG..................................................... 21 1 1 1.00 1 0 0 0 1 0 0
.....................................................................................AAGACTGTTTGAAGTGCCTGAGA.................................................... 23 1 1 1.00 1 1 0 0 0 0 0
......................................................................................AGACTGTTTGAATTGCCTGGGA.................................................... 22 1 1 1.00 1 1 0 0 0 0 0
......................................................................................AGACTGTTCGAAGTGCCTGGGA.................................................... 22 1 1 1.00 1 0 0 1 0 0 0
.....................................................................................AAGACTGTTTGAAGTGCCCGGGA.................................................... 23 1 1 1.00 1 1 0 0 0 0 0
......................................................................................AGACTGTTTGAATTGCCTGGGAA................................................... 23 1 1 1.00 1 0 1 0 0 0 0
..................................................CCAAGTCCTTTTTAGAGTGCT......................................................................................... 21 1 1 1.00 1 0 1 0 0 0 0
.......................................................................................GACTGTTTGAAGTGCCTGGGAAAA................................................. 24 2 1 1.00 1 1 0 0 0 0 0
.....................................................................................AAGACTGTTTAAAGTGCCTTGTA.................................................... 23 3 1 1.00 1 0 0 0 0 0 1
......................................................................................ACACTGTTTGAAGTCCCTCGGA.................................................... 22 3 1 1.00 1 0 0 0 1 0 0
......................................................................................AGACTGTTTGAAGTGCCTGGTA.................................................... 22 1 1 1.00 1 1 0 0 0 0 0
......................................................................................ACACTGTTTGAAGTGCCTGGGAA................................................... 23 1 1 1.00 1 1 0 0 0 0 0
......................................................................................AGGCTGTTGGAAGTGCCTGGGCA................................................... 23 3 1 1.00 1 0 0 0 1 0 0
......................................................................................AGACTGTTTGAAGGGCCTGGGA.................................................... 22 1 1 1.00 1 0 0 0 1 0 0
......................................................................................AGACTGTTTGAAGTGCCTGGGATTCG................................................ 26 3 1 1.00 1 1 0 0 0 0 0
......................................................................................TCACTGTTTGAAGTGCCTGGGA.................................................... 22 2 1 1.00 1 0 1 0 0 0 0
......................................................................................AGACTGTTTGAAGTGCCTGGGAG................................................... 23 1 1 1.00 1 0 0 1 0 0 0
..................................................CCAAGGCCTTTTTAGAGTCCT......................................................................................... 21 1 1 1.00 1 0 0 0 1 0 0
......................................................................................AGACTGTTTGAAGTGCCTTGGA.................................................... 22 1 1 1.00 1 1 0 0 0 0 0
.....................................................................................AAGACTGTTTGAAGTGCCTGCGA.................................................... 23 1 1 1.00 1 0 0 0 1 0 0
.....................................................................................AACACTGTTTGAAGTGCCTGG...................................................... 21 1 1 1.00 1 0 0 1 0 0 0
......................................................................................AGACTGTTTGAAGTGCCTGGT..................................................... 21 1 1 1.00 1 1 0 0 0 0 0
......................................................................................AGACTGTTTGAAGTGCCTGGGAAG.................................................. 24 1 1 1.00 1 0 0 1 0 0 0
...........................................................................................GTTTGAAGTGCCTGGGAAT.................................................. 19 0 1 1.00 1 0 1 0 0 0 0
.......................................................................................GACTGTTTGAAGTGCCTGGGAAA.................................................. 23 1 1 1.00 1 1 0 0 0 0 0
...............................................ATTCCAAGTCCTTTTTAGAGTC........................................................................................... 22 0 1 1.00 1 0 0 1 0 0 0
......................................................................................AGACTGTTTGAAGTTCCTGGGAA................................................... 23 1 1 1.00 1 1 0 0 0 0 0
........................................................................................ACTGTTTGAAGTGCCTGGGAAA.................................................. 22 1 1 1.00 1 1 0 0 0 0 0
...................................................CAAGTCCTTTTTAGAGTCCT......................................................................................... 20 0 1 1.00 1 0 0 1 0 0 0
......................................................................................AGACTGTTTGAAGTGCCTGGGAC................................................... 23 1 1 1.00 1 0 0 1 0 0 0
......................................................................................AGACTGTTTGAAATGCCTGGGA.................................................... 22 1 1 1.00 1 0 1 0 0 0 0
.....................................................................................AAGACTGTTTGAAGTTCCTGGGATT.................................................. 25 2 1 1.00 1 1 0 0 0 0 0
.......................................................................................GACTGTTTGAAGTTCCTGGGA.................................................... 21 1 1 1.00 1 1 0 0 0 0 0
.....................................................................................AAGACTGTTGGAAGCGCCTGCGA.................................................... 23 3 1 1.00 1 0 0 0 1 0 0
......................................................................................AGACTGTTTGACGTGCCTGGGAAT.................................................. 24 1 1 1.00 1 0 1 0 0 0 0
......................................................................................AGACTGTTTGAACTGCCTGGGAA................................................... 23 1 1 1.00 1 0 1 0 0 0 0
......................................................................................AGACTGTTTGAAGTGCCTGGGC.................................................... 22 1 1 1.00 1 0 0 0 1 0 0
.....................................................................................AAGACTGTTTGAAGTGCCTGGGATT.................................................. 25 1 1 1.00 1 0 0 0 1 0 0
......................................................................................AGACTGTTGGAAGTGCCTGGGA.................................................... 22 1 1 1.00 1 0 0 1 0 0 0
......................................................................................AGACTGTTTGAGGTGACTGGGAA................................................... 23 2 1 1.00 1 1 0 0 0 0 0
......................................................................................AGACTGTTTGAAGTGCCTGGTAA................................................... 23 1 1 1.00 1 1 0 0 0 0 0
......................................................................................AGACTGTTTGAATTGCCTGGGATT.................................................. 24 2 1 1.00 1 0 1 0 0 0 0
..................................................CCAAGTCCTTTTTAGAGTCCTTT....................................................................................... 23 0 1 1.00 1 0 1 0 0 0 0
......................................................................................AGACTGCTTGAAGCGCCTGGGC.................................................... 22 3 2 0.50 1 0 0 0 1 0 0
..................................................CCAAGTCCTTTTTCGAGACCT......................................................................................... 21 2 2 0.50 1 0 1 0 0 0 0
....................................................................................AAAAACTGTTTGAATTGCC......................................................... 19 2 13 0.08 1 0 0 0 0 1 0

Anti-sense strand reads

ATTTTACTTTTATATAGCTTACACGTTCTTTGAGTTTTATTTTCAACTAAGGTTCAGGAAAAATCTCAGGAAAAACTTTACTTCTTTCTGACAAACTTCACGGACCCTTAATTAAAACTATAAATTCGTTGTCAAAACTTTCACTTCTTACTTATTCATG

************************************.....((((((((((((..((.((((....((((.(((((......))))).))))....)))).))...)))))))))))).......*********************************** 		Read size # Mismatch Hit Count Total Norm Total M044

female body

 V055

head

 V039

embryo
....................................................GTCAGGAAAGATCTCACGAAAA...................................................................................... 22 3 2 0.50 1 0 1 0
.......................................................AGGAAGAATCTCAGGAAAAA..................................................................................... 20 1 2 0.50 1 1 0 0
....................................................................................................................................CCAAAGTTTCACTTCTTTCTT....... 21 3 10 0.10 1 0 0 1

Re-alignment of all predicted orthologs

Species Coordinate ID Type Alignment
droAna3 scaffold_12903:590466-590625 + dan_52 confident TAAAATGAAAATATA-------------TCGAATGTGCAAGAAACTCAAAATAAAAGTTGATTCCAAGTCCTTTTTAGAGTCCTTTTTGAAATGAAGAAAGACTGTTTGA----AGTGCCTGGGAATTAATTTTGATATTTAAGCAACAG----TTTTGAAAGTGAAGAATGAATAAGTAC---------------------------
droBip1 scf7180000395925:18902-19079 - TAATATGGTAACTTTTTATCCCCAAATATCGCATTTCG------------ATAAAAATAGAATTCAAATG------------------ATAAAGCAGAAAGCTTTTTTAATATATGTACCTCAGATTTAATCTCAATATTTAAGCATCTCTGCATATCAAAACCAAAGAAACTATAAATAGGCAACAATTATAAACCTTTTTGTTTAA
droEre2 scaffold_4690:9468181-9468183 - TAC-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droAna3
TAAAATGAAAATATA-------------TCGAATGTGCAAGAAACTCAAAATAAAAGTTGATTCCAAGTCCTTTTTAGAGTCCTTTTTGAAATGAAGAAAGACTGTTTGA----AGTGCCTGGGAATTAATTTTGATATTTAAGCAACAG----TTTTGAAAGTGAAGAATGAATAAGTAC---------------------------SizeHit CountTotal NormTotalM044

Female-body		M058

Embryo		V039

Embryo		V055

Head		V105

Male-body		V106

Head
...................................................................................................AGACTGTTTGA----AGTGCCTGGGAA..................................................................................231160.00160281172994
...................................................................................................AGACTGTTTGA----AGTGCCTGGGA...................................................................................221152.001522121132689
...............................................................CCAAGTCCTTTTTAGAGTCCT............................................................................................................................21145.004513102236
..................................................................................................AAGACTGTTTGA----AGTGCCTGGGA...................................................................................23123.002320011010
....................................................................................................GACTGTTTGA----AGTGCCTGGGAA..................................................................................22116.00161000312
...................................................................................................AGACTGTTTGA----AGTGCCTGGGAT..................................................................................23113.0013100336
...................................................................................................AGACTGTTTGA----AGTGCCTGGGAAT.................................................................................2419.009000126
....................................................................................................GACTGTTTGA----AGTGCCTGGGA...................................................................................2116.006200004
...................................................................................................AGACTGTTTGA----AGTGCCTGGGAAA.................................................................................2415.005000005
...............................................................CCAAGTCCTTTTTAGAGTCC.............................................................................................................................2014.004200110
....................................................................................................GACTGTTTGA----AGTGCCTGGGAAT.................................................................................2313.003000012
...................................................................................................AGACTGTTTGA----AGTGCCTGGGT...................................................................................2213.003000102
...................................................................................................AGACTGTTTGA----AGTGCCTGGG....................................................................................2113.003000003
...............................................................CCAAGTCCTTTTTAGAGTCCTT...........................................................................................................................2213.003200010
...............................................................CCAAGTCCTTTTTAGAGTC..............................................................................................................................1912.002100001
..................................................................................................AAGACTGTTTGA----AGTGCCTGG.....................................................................................2112.002100010
.....................................................................................................ACTGTTTGA----AGTGCCTGGGAA..................................................................................2112.002000002
...................................................................................................AGACTGTTTGA----AGTGCCTGGGAAC.................................................................................2412.002000002
.............................................................TTCCAAGTCCTTTTTAGAGTCC.............................................................................................................................2211.001100000
...................................................................................................AGACTGTTTGA----AGTGCCTGGGATC.................................................................................2411.001000001
................................................................................................GAAAGACTGTTTGA----AGTGCCTG......................................................................................2211.001100000
..................................................................................................AAGACTGTTTGA----AGTGCCTG......................................................................................2011.001100000
.....................................................................................................ACTGTTTGA----AGTGCCTGGGG...................................................................................2011.001000001
....................................................................................................GACTGTTTGA----AGTGCCTGGGAAAA................................................................................2411.001000001
...................................................................................................AGACTGTTTGA----AGTGCCTGGGAG..................................................................................2311.001100000
...................................................................................................AGACTGTTTGA----AGTGCCTGGT....................................................................................2111.001000001
...................................................................................................AGACTGTTTGA----AGTGCCTGGGAAG.................................................................................2411.001100000
........................................................................................................GTTTGA----AGTGCCTGGGAAT.................................................................................1911.001000010
....................................................................................................GACTGTTTGA----AGTGCCTGGGAAA.................................................................................2311.001000001
............................................................ATTCCAAGTCCTTTTTAGAGTC..............................................................................................................................2211.001100000
.....................................................................................................ACTGTTTGA----AGTGCCTGGGAAA.................................................................................2211.001000001
................................................................CAAGTCCTTTTTAGAGTCCT............................................................................................................................2011.001100000
...................................................................................................AGACTGTTTGA----AGTGCCTGGGAC..................................................................................2311.001100000
...................................................................................................AGACTGTTTGA----AGTGCCTGGGC...................................................................................2211.001000100
...............................................................CCAAGTCCTTTTTAGAGTCCTTT..........................................................................................................................2311.001000010
droBip1
TAATATGGTAACTTTTTATCCCCAAATATCGCATTTCG------------ATAAAAATAGAATTCAAATG------------------ATAAAGCAGAAAGCTTTTTTAATATATGTACCTCAGATTTAATCTCAATATTTAAGCATCTCTGCATATCAAAACCAAAGAAACTATAAATAGGCAACAATTATAAACCTTTTTGTTTAASizeHit CountTotal NormTotal
No Reads
droEre2
TAC-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SizeHit CountTotal NormTotalM055

Female-body		M057

Embryo		V060

Head		V040

Embryo		V107

Male-body		V108

Head		GSM1528801

follicle cells
No Reads

Generated: 10/20/2015 at 07:29 PM