dan_3375

##################################################--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- AGCAACAGCAGCAGAACAGTCGGAGCAGCGTAGGACCGCAGCCGGAGCACGTGAGTCCGAAAGTGAGAGAATAGTTATCTCTGTAAGATAACCGCTGCATTTGTTGTGAGCATAAACAAACCAAAACAGTTCGTTACACCTGGCGCCCAAAATAAGCGATTGCATTCGAGTCTAGAAATTATGCAGCTTTAAGAAGGGGAGGAAAAATATATAGGCGCTGCGCAAGGAGGAGCTGACTGAAGTGTGTAGAG ************************************************.....(((.......((((....(((((((.....)))))))((((...((((.(((.((....(((.(((.......))).)))....)).)))..))))...)))).((((((((.......))....)))))))))).......))).************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M044 female body	V055 head	M058 embryo	V105 male body	V039 embryo
.................................................................................................................CAACAAACCAAGGCAGTTCGT.....................................................................................................................	21	3	2	3.00	6	1	0	2	2	1
....................................................................................................................................................................................ATGCAGCTCTAAGAGGGGG....................................................	19	2	1	1.00	1	0	1	0	0	0
.....................................................................................................................................................................................................GGAGGAAAAATATATTGGCGCTG...............................	23	1	1	1.00	1	1	0	0	0	0
................CAGTCGGAGCAGCGTAGGACCGCAG..................................................................................................................................................................................................................	25	0	1	1.00	1	1	0	0	0	0
......................GACCAACGTAGCACCGCAGCC................................................................................................................................................................................................................	21	3	2	0.50	1	0	1	0	0	0
..................................................................................................................AACAAACCAAGGCAGTTCGT.....................................................................................................................	20	2	2	0.50	1	1	0	0	0	0
..................................................................................................................AACAAACCAAGGCAGTTCGTA....................................................................................................................	21	3	3	0.33	1	1	0	0	0	0
............................................................................................................................................................................................TTAAGAAGGGGAGTGAGAATA..........................................	21	3	6	0.17	1	0	1	0	0	0
...........................................................................................................................................CTAGTGCCCAAAATATGCGAT...........................................................................................	21	3	8	0.13	1	1	0	0	0	0
.........AGCAGAACAGTCGGCCAAGC..............................................................................................................................................................................................................................	20	3	9	0.11	1	0	0	1	0	0
..................................................................................................................................................................................................AGGGGAGCAAAAATACAT.......................................	18	2	13	0.08	1	0	0	1	0	0
..........................................................................................................................................................................TCTAGAAAATATGCTGCT...............................................................	18	2	14	0.07	1	0	1	0	0	0
.......................................................................................................................................................................................CAGCTTTAGAGAGGGGAGG.................................................	19	3	15	0.07	1	1	0	0	0	0
.......................................................................................................................................................................................................................................GATGACTGAAGTGGGTAAAG	20	3	16	0.06	1	0	0	0	1	0
................................................................................................................................GTTCGTTACACCTGGCGC.........................................................................................................	18	0	20	0.05	1	1	0	0	0	0
.......................................................................................ATAACCGCCGCGTTTGTGG.................................................................................................................................................	19	3	20	0.05	1	0	0	1	0	0
...........................................................AACGTGAGAGGATGGTTATC............................................................................................................................................................................	20	3	20	0.05	1	0	1	0	0	0

TCGTTGTCGTCGTCTTGTCAGCCTCGTCGCATCCTGGCGTCGGCCTCGTGCACTCAGGCTTTCACTCTCTTATCAATAGAGACATTCTATTGGCGACGTAAACAACACTCGTATTTGTTTGGTTTTGTCAAGCAATGTGGACCGCGGGTTTTATTCGCTAACGTAAGCTCAGATCTTTAATACGTCGAAATTCTTCCCCTCCTTTTTATATATCCGCGACGCGTTCCTCCTCGACTGACTTCACACATCTC ************************************************.....(((.......((((....(((((((.....)))))))((((...((((.(((.((....(((.(((.......))).)))....)).)))..))))...)))).((((((((.......))....)))))))))).......))).************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M044 female body	V055 head	M058 embryo	V039 embryo
.............................................................................................................................................................................................................................CGTTCCTCCTCGACTGACTTCACACAT...	27	0	1	1.00	1	1	0	0	0
......................................................................................................................................................................................CGTCGACAATCTTCCCCTCCTT...............................................	22	2	1	1.00	1	0	1	0	0
............................................................................................................................TTGTCAAGCAATGTGGACCGCGGGTTT....................................................................................................	27	0	20	0.85	17	17	0	0	0
....................................................................................................................................CAATGTGGACCGCGGGTTTT...................................................................................................	20	0	4	0.25	1	1	0	0	0
.................................................................................................................................................................................................TTCCCCTCGTTTTTATGTA.......................................	19	2	4	0.25	1	0	0	1	0
..............................................CGGGCACTAAGCCTTTCACT.........................................................................................................................................................................................	20	3	8	0.13	1	1	0	0	0
....................................................................................................................GTTTGGTTCCGTCAAGCA.....................................................................................................................	18	2	8	0.13	1	0	0	1	0
....................................................................................................................................................................................................................TCCGCGACGCGAGACTCCTC...................	20	3	8	0.13	1	0	0	0	1
..........................................................................................................................TTTTGTCAAGCAATGTGGACCGCGGGTT.....................................................................................................	28	0	20	0.10	2	2	0	0	0
...................................................................................GTTCTATTGGCGACATAAG.....................................................................................................................................................	19	3	19	0.05	1	0	1	0	0
.................................................................................................................GTTGTTTGGTTCCGTCAAG.......................................................................................................................	19	3	20	0.05	1	0	0	1	0

Species	Coordinate	ID	Alignment
droAna3	scaffold_13499:1582827-1583077 -	dan_3375	AGCAACAGCAGCAGAACAGTCGGAGCAGCGTAGGACCGCAGCCGGAGCACGTGAGTCCGAAAGTGAGAGAATAGTTATCTCTGTAAGATAACCGCTGCAT-T-TGTTGTGAGCATAAACAAACC-AAAACAGTTCGTTACACCTGGCGCCCAAAA-------------------------------------TAAGCGATTGCA------------TTCGAGTCTA-------------------------GAAATTATGCAGCTTTAAGAAGGGGAGGAAAAAT---ATATAGGCGCTGCGCAAGGAGGAGCTGACTGAAGTGTGTAGAG
droBip1	scf7180000396559:10694-10869 +		C------------------------------------------------------------------------------TCTGTGAGCTAGCCGCGGCATAC-TGTGGCGAGCGGAAGCA-AAG-AAAACAGTTCGTTACACCTGGCGCCCAAAC-------------------------------------TTCGTGATTGCATTT-------------AGCCTAGAAAATAA------------------------CGTAGCAGAAAGATGGGAAGAAAGAATTGGATCTATACATTGAGAAAGGAGGAGCTGAGCGAAGTGTGTACAG
droFic1	scf7180000454177:13460-13514 -		-----------------------------------------------------------------------------------TGAGCTAGCCGCA-CGTTA-CGTAGCGAGCGGAGACATATA-AAAGCAGTTCGTTACA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEle1	scf7180000491275:3222-3300 +		AA-------------------------------------------------------------------------------------------------------------------ATAAACAAAAATCAATTCGTTACATCTGGCGCCCAAAA---------------------------------------CGTGACTGCCCTGACTGTAAGATTCAGGTCAA-------------------------AACATTA-------------------------------------------------------------------------
droRho1	scf7180000773242:26162-26330 -		AC----------------------------------------------------------------------------CTCTCTGAGCTAGCCGCACGTTTA-CGTAGCGAGCAGAAACT-AAA-GAATCAGGTCGTTACATCTGGCGCCCAAGA----------------------------------------GTGATTGTCCTG-------------ACCAAGGAAACAAA---------------------------AGTTCCGAGATGGGCAA---GTCCTGGATTTATAATCTCAGGAAGGAGGACTTCGATGAGGTGAGTCAAG
droBia1	scf7180000302262:115175-115267 -		G-----------------------------------------------------------------------------------------------------------CGAGCAGAACGT-ACA-AAATCAGTTCGTTACATCTGGCGCCCAAAA---------------------------------------CGTGATTGTCCCAGTAGTG--------------AAAGCAACTTAAACAGATTGGGAAAGAA----------------------------------------------------------------------------
droTak1	scf7180000414193:14072-14174 +		TA-----------------------------------------------------------------------------TCTCTGAGCTAGTCGCGGCGTTTACGTAGCGATCA--AACAGTTG-AAAACAGTTCGTTACAGC----ATCTAGAT-------------------------------------GAAGCTGAAATA------------TGC--GT-TA-------------------------AAAACTTTGAAGC-------------------------------------------------------------------
dm3	chr3RHet:23067-23140 -		AT----------------------------------------------------------------------------CTTTCTGAGCTAGCCGTTGCACTT-CGTAACGAGCCGAAATA-GCA-AAAACTGTTTCTTACATCTGGCGCCCAA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droSec2	scaffold_160:14267-14338 +		C------------------------------------------------------------------------------TCTCTGAGCTAGCCGTTGCAACT-CGTAGCGAACAGAGACA-AGA-AAAACAGTTCGTTACATCTGGCGCACAA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droYak3	v2_chrUn_4013:245-383 -		C------------------------------------------------------------------------------TCTCTGAGCTAGCCGTTGCATTT-CGTAGCGAGCAGAAACA-AGG-AAAACAGTTCGTTACATCTGGCGCCCAACGTGGGGCAAGGCGAGCGGGTCATATCCTACTCCAGCAGAGCT---------------------------------------CTGAACGGAGCGGAGAAAAATTAT------------------------------------------------------------------------
dp5	Unknown_group_5:37025-37061 -		AG-------------------------------------------------------------------------------------------------------------------ACGGAAC-AGAACGGTTCGTTACAACTGGCGCCCAA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droPer2	scaffold_989:3350-3386 +		AG-------------------------------------------------------------------------------------------------------------------ACGGAGA-AGAACGGTTCGTTACAACTGGCGCCCAA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droWil2	scf2_1100000004913:128725-128773 -		AA----------------------------------------------------------------------------CAA-CTGCAG------------------------CAGCAACA-ACA-ACAGCAGCA-ACAACAACAGCAGCAGCA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droMoj3	scaffold_6187:799-832 -		AG--------------------------------------------------------------------------------------------------------------CAACAACA-GCA-GCAGCAGG-------CCCAGAAGTACAG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droAna3

AGCAACAGCAGCAGAACAGTCGGAGCAGCGTAGGACCGCAGCCGGAGCACGTGAGTCCGAAAGTGAGAGAATAGTTATCTCTGTAAGATAACCGCTGCAT-T-TGTTGTGAGCATAAACAAACC-AAAACAGTTCGTTACACCTGGCGCCCAAAA-------------------------------------TAAGCGATTGCA------------TTCGAGTCTA-------------------------GAAATTATGCAGCTTTAAGAAGGGGAGGAAAAAT---ATATAGGCGCTGCGCAAGGAGGAGCTGACTGAAGTGTGTAGAG	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
................CAGTCGGAGCAGCGTAGGACCGCAG..................................................................................................................................................................................................................................................................................................	25	1	1.00	1	1	0	0	0	0	0
...................................................................................................................................GTTCGTTACACCTGGCGC......................................................................................................................................................................................	18	20	0.05	1	1	0	0	0	0	0

droBip1

C------------------------------------------------------------------------------TCTGTGAGCTAGCCGCGGCATAC-TGTGGCGAGCGGAAGCA-AAG-AAAACAGTTCGTTACACCTGGCGCCCAAAC-------------------------------------TTCGTGATTGCATTT-------------AGCCTAGAAAATAA------------------------CGTAGCAGAAAGATGGGAAGAAAGAATTGGATCTATACATTGAGAAAGGAGGAGCTGAGCGAAGTGTGTACAG	Size	Hit Count	Total Norm	Total
No Reads

droFic1

-----------------------------------------------------------------------------------TGAGCTAGCCGCA-CGTTA-CGTAGCGAGCGGAGACATATA-AAAGCAGTTCGTTACA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEle1

AA-------------------------------------------------------------------------------------------------------------------ATAAACAAAAATCAATTCGTTACATCTGGCGCCCAAAA---------------------------------------CGTGACTGCCCTGACTGTAAGATTCAGGTCAA-------------------------AACATTA-------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droRho1

AC----------------------------------------------------------------------------CTCTCTGAGCTAGCCGCACGTTTA-CGTAGCGAGCAGAAACT-AAA-GAATCAGGTCGTTACATCTGGCGCCCAAGA----------------------------------------GTGATTGTCCTG-------------ACCAAGGAAACAAA---------------------------AGTTCCGAGATGGGCAA---GTCCTGGATTTATAATCTCAGGAAGGAGGACTTCGATGAGGTGAGTCAAG	Size	Hit Count	Total Norm	Total
No Reads

droBia1

G-----------------------------------------------------------------------------------------------------------CGAGCAGAACGT-ACA-AAATCAGTTCGTTACATCTGGCGCCCAAAA---------------------------------------CGTGATTGTCCCAGTAGTG--------------AAAGCAACTTAAACAGATTGGGAAAGAA----------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droTak1

TA-----------------------------------------------------------------------------TCTCTGAGCTAGTCGCGGCGTTTACGTAGCGATCA--AACAGTTG-AAAACAGTTCGTTACAGC----ATCTAGAT-------------------------------------GAAGCTGAAATA------------TGC--GT-TA-------------------------AAAACTTTGAAGC-------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

dm3

AT----------------------------------------------------------------------------CTTTCTGAGCTAGCCGTTGCACTT-CGTAACGAGCCGAAATA-GCA-AAAACTGTTTCTTACATCTGGCGCCCAA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	GSM154618 Ovary	GSM154620 Ovary	GSM154621 Ovary	GSM154622 Ovary	GSM180328 Head	GSM180329 Mixed-body	GSM180330 Embryo	GSM180331 Embryo	GSM180332 Embryo	GSM180333 Embryo	GSM180334 Larvae	GSM180335 Disc	GSM180336 Pupae	GSM180337 S2	GSM231091 Ovary	GSM239041 Head	GSM239050 Head	GSM239051 S2	GSM239052 S2	GSM246084 Head	GSM266765 S2	GSM280082 Ovary	GSM280083 Ovary	GSM280084 Ovary	GSM280085 Testis	GSM280086 Ovary	GSM280087 S2-NP	GSM280088 S2-NP	GSM312995 Head	GSM313160 Head	GSM313161 Head	GSM313162 Head	GSM313163 Head	GSM313164 Head	GSM313165 Head	GSM343287 Embryo	GSM379050 Ovary	GSM379051 Ovary	GSM379052 Ovary	GSM379053 Ovary	GSM379054 Ovary	GSM379055 Ovary	GSM379056 Ovary	GSM379057 Ovary	GSM379058 Ovary	GSM379059 Ovary	GSM379060 Ovary	GSM379061 Ovary	GSM379062 Ovary	GSM379063 Ovary	GSM379064 Ovary	GSM379065 Ovary	GSM379066 Ovary	GSM379067 Ovary	GSM424739 S2	GSM424740 S2	GSM424741 S2	GSM467729 Female-body	GSM467730 Female-body	GSM467731 Female-body	M002 CS	M003 Male-body	M004 Male-body	M005 ML-DmD32	M006 ML-DmD32	M029 OSC	M030 OSC	M031 OSC	M032 S2-R+	M033 S2-R+	M034 S2-R+	M035 S2-R+	M036 S2-R+	M037 S2-R+	M038 S2-R+	M039 S2-R+	M048 S2-R+	OSS2 OSS	OSS6 OSS	OSS7 OSS	OSS8 OSS	S1 Head	S10 Embryo	S11 Embryo	S12 Embryo	S13 Embryo	S14 Embryo	S15 S2-GMR	S16 Kc	S17 Larvae	S18 Larvae	S19 Pupae	S2 Head	S20 Pupae	S21 Disc	S22 Head	S23 Female-body	S24 Male-body	S25 Larvae	S26 Larvae	S27 Pupae	S28 Pupae	S29 Head	S3 Male-body	S30 S2-GMR	S31 S2	S32 S2	S33 Kc	S34 Kc	S35 Kc	S4 Female-body	S5 Disc	S6 Embryo	S7 Embryo	S8 S2	S9 Embryo	SRR001337 Head	SRR001338 Head	SRR001339 Head	SRR001340 Head	SRR001341 Head	SRR001342 Head	SRR001343 Head	SRR001344 Head	SRR001345 Head	SRR001346 Head	SRR001347 Head	SRR001348 Head	SRR001349 Head	SRR001664 Head	SRR010951 Ovary	SRR010952 Ovary	SRR010953 Ovary	SRR010954 Ovary	SRR010955 Ovary	SRR010956 Ovary	SRR010957 Ovary	SRR010958 Ovary	SRR010959 Ovary	SRR010960 Ovary	SRR014268 Embryo	SRR014269 Embryo	SRR014270 Embryo	SRR014271 Embryo	SRR014272 Embryo	SRR014273 Ovary	SRR014274 Ovary	SRR014275 Ovary	SRR014276 Ovary	SRR014277 Ovary	SRR014278 Ovary	SRR014279 Ovary	SRR014280 Ovary	SRR014281 Ovary	SRR014282 Ovary	SRR023197 S2	SRR023399 S2	SRR023400 S2	SRR023402 S2	SRR023407 S2	SRR029028 S2	SRR029029 S2	SRR029030 S2	SRR029031 S2	SRR029032 S2	SRR029033 S2	SRR029608 Head	SRR029633 Head	SRR030145 gb3	SRR031692 Head	SRR031693 Head	SRR031694 Head	SRR031696 Head	SRR031697 Head	SRR031698 Head	SRR031699 Head	SRR031700 Head	SRR031701 Head	SRR031702 Head	SRR031703 Head	SRR031704 Head	SRR032092 S2	SRR032093 S2	SRR032094 S2	SRR032095 S2-NP	SRR032096 S2-NP	SRR032152 Head	SRR060643 Testis	SRR060644 Ovary	SRR060645 Testis	SRR060646 Ovary	SRR060647 Ovary	SRR060648 Ovary	SRR060649 Ovary	SRR060650 Testis	SRR060651 Ovary	SRR060652 Testis	SRR060653 Ovary	SRR065151 Ovary	SRR065152 Ovary	SRR065153 Ovary	SRR065154 Ovary	SRR065155 Ovary	SRR065800 Ovary	SRR065801 Ovary	SRR065802 Ovary	SRR065803 Ovary	SRR065804 Ovary	SRR065805 Ovary	SRR065806 Ovary	SRR065807 Ovary	SRR097865 S2-NP	SRR097866 S2-NP	SRR097867 S2-NP	SRR298536 Ovary	SRR298537 Ovary	SRR298711 Ovary	SRR298712 Ovary	SRR317110 Ovary	SRR317111 Ovary	SRR317112 Ovary	SRR317113 Ovary	SRR317114 Ovary	SRR317115 Ovary	SRR317116 Ovary	SRR317117 Ovary	SRR341115 S2	SRR341116 S2	SRR341117 S2	SRR341118 S2	SRR351332 Mixed-body	SRR351333 Mixed-body	T004 OSC	T005 CME-L1	V001 Embryo	V002 Embryo	V003 S2	V006 Female-body	V007 Head	V008 S2	V009 CME-L1	V010 ML-DmD20-C5	V011 Sg4	V012 Male-body	V013 Head	V014 DTT	V015 DreRFHV	V018 Kc167	V019 GM2	V020 S2-R+	V021 ML-DmD21	V022 ML-DmD32	V023 Head	V024 Kc167	V025 S2-R+	V026 1182-2H	V027 ML-DmD21	V028 CME-W1-Cl.8+	V029 ML-DmD9	V030 ML-DmD8	V031 GM2	V032 S1	V033 CME-W1-Cl.8+	V034 ML-DmD16-C3	V035 ML-DmD32	V036 ML-DmD20-C5	V037 S2	V038 S2	V063 Ovary	V0632 Ovary	V064 Ovary	V0642 Ovary	V065 Ovary	V0652 Ovary	V066 Ovary	V0662 Ovary	V067 S2-NP	V073 mbn2	V074 S3	V075 ML-DmBG1-C1	V076 ML-DmBG3-C2	V077 Head	V078 Head	V079 Head	V080 Head	V081 Embryo	V082 Embryo	V083 Male-body	V084 Female-body	V085 CME-W2	V086 Female-body	V088 Ovary	V0882 Ovary	V089 Ovary	V0892 Ovary	V090 Male-body	V091 fGS/OSS	V092 Male-body	V093 Male-body	V094 S2-R+	V095 S2-R+	V096 Ovary	V097 Ovary	V098 Ovary	V125 ML-DmD9	V126 CME-L1	V127 G2	V128 S3	V129 ML-DmBG1-C1	V130 ML-DmBG3-C2	V131 ML-DmD16-C3	V132 ML-DmD32	V133 Sg4	V134 ML-DmD8	V135 CME-W2	V136 Male-body	V137 Head	V138 Male-body	V139 Female-body	V140 Female-body	V141 Female-body	V142 Female-body	V144 OSC	V145 S2	V146 S1	V147 1182-2H	V148 mbn2	GSM1528798 follicle cells
.T----------------------------------------------------------------------------CTTTCTGAGCTAGCCGTTGCACT......................................................................................................................................................................................................................................	24	1	81.00	81	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	4	0	0	0	1	0	0	1	0	0	0	3	0	0	0	0	0	8	0	0	1	4	1	10	0	6	8	13	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	1	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	2	3	3	0	0	0	0	0	0
...........................................................................................................ACGAGCCGAAATA-GCA-AAAACTGTT.....................................................................................................................................................................................................	25	1	33.00	33	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	5	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	2	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	3	0	1	0	0	0	0	0	0	0	3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	4	4	3	0	0	0	0	0	0
...................................................................................TGAGCTAGCCGTTGCACTT-CGTAACGA............................................................................................................................................................................................................................	27	1	30.00	30	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	4	0	0	0	0	0	0	1	0	0	0	1	0	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	2	0	1	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	1	3	1	0	0	0	0	0	9
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................................................................................TTCTGAGCTAGCCGTTGCACTT-CGTA................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0
.................................................................................................................CGAAATA-GCA-AAAACTGT......................................................................................................................................................................................................	18	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..............................................................................................TTGCACTT-CGTAACGAGCCG........................................................................................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................CTGAGCTAGCCGTTGCACTT-CGTAAA..............................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droSec2

C------------------------------------------------------------------------------TCTCTGAGCTAGCCGTTGCAACT-CGTAGCGAACAGAGACA-AGA-AAAACAGTTCGTTACATCTGGCGCACAA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
...............................................................................................TGCAACT-CGTAGCGAACAGAGACA-A.................................................................................................................................................................................................................	25	6	0.17	1	0	0	1	0
................................................................................................................CAGAGACA-AGA-AAAACAGTTCGTTACATC............................................................................................................................................................................................	29	6	0.17	1	1	0	0	0
......................................................................................................................CA-AGA-AAAACAGTTCGTTACATC............................................................................................................................................................................................	23	6	0.17	1	1	0	0	0
................................................................................CTCTGAGCTAGCCGTTGCAACT-CGT.................................................................................................................................................................................................................................	25	8	0.13	1	1	0	0	0

droYak3

C------------------------------------------------------------------------------TCTCTGAGCTAGCCGTTGCATTT-CGTAGCGAGCAGAAACA-AGG-AAAACAGTTCGTTACATCTGGCGCCCAACGTGGGGCAAGGCGAGCGGGTCATATCCTACTCCAGCAGAGCT---------------------------------------CTGAACGGAGCGGAGAAAAATTAT------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M026 Head	M043 Female-body	M056 Embryo	V046 Embryo	V052 Head	V058 Head	V120 Male-body	SRR1275488 Male larvae	SRR1275486 Male prepupae	SRR1275484 Male prepupae	GSM1528802 follicle cells
...............................................................................................................GCAGAAACA-AGG-AAAACAGTTCGTT.................................................................................................................................................................................................	25	9	1.44	13	0	9	4	0	0	0	0	0	0	0	0
...............................................................................................................GCAGAAACA-AGG-AAAACAGTTCGTTAC...............................................................................................................................................................................................	27	9	1.33	12	0	3	9	0	0	0	0	0	0	0	0
..................................................................................................ATTT-CGTAGCGAGCAGAAACA-AGG-AT............................................................................................................................................................................................................	26	15	0.80	12	0	0	0	0	0	0	0	0	0	0	12
..............................................................................................................AGCAGAAACA-AGG-AAAACAGTTCGT..................................................................................................................................................................................................	25	9	0.67	6	0	1	1	1	0	0	3	0	0	0	0
................................................................................CTCTGAGCTAGCCGTTGCATTT-CG..................................................................................................................................................................................................................................	24	20	0.65	13	0	13	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................TCCTACTCCAGCAGAGCT---------------------------------------CTGAACGGAT......................................................................................	28	12	0.58	7	0	0	0	0	0	0	0	0	0	0	7
.............................................................................................................................................................................TCATATCCTACTCCAGCAGAGCT---------------------------------------C...............................................................................................	24	12	0.50	6	0	6	0	0	0	0	0	0	0	0	0
.............................................................................................................................................................................TCATATCCTACTCCAGCAGAGCT---------------------------------------CT..............................................................................................	25	12	0.50	6	0	6	0	0	0	0	0	0	0	0	0
...................................................................................................................AAACA-AGG-AAAACAGTTCGTTACATC............................................................................................................................................................................................	26	4	0.50	2	0	1	1	0	0	0	0	0	0	0	0
........................................................................................................................................................................................TCCAGCAGAGCT---------------------------------------CTGAACGGAGCGGA..................................................................................	26	14	0.29	4	0	4	0	0	0	0	0	0	0	0	0
....................................................................................................................AACA-AGG-AAAACAGTTCGTTACATC............................................................................................................................................................................................	25	4	0.25	1	0	0	1	0	0	0	0	0	0	0	0
......................................................................................................................CA-AGG-AAAACAGTTCGTTACATCTGGC........................................................................................................................................................................................	27	4	0.25	1	0	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................A-AGG-AAAACAGTTCGTTACATCTGGCGC......................................................................................................................................................................................	28	4	0.25	1	0	1	0	0	0	0	0	0	0	0	0
........................................................................................................................................................................................TCCAGCAGAGCT---------------------------------------CTGAACGGAGCGGAG.................................................................................	27	8	0.25	2	0	1	0	0	0	0	1	0	0	0	0
.........................................................................................................................AGG-AAAACAGTTCGTTACATCTGGCGC......................................................................................................................................................................................	27	4	0.25	1	0	1	0	0	0	0	0	0	0	0	0
..................................................................................................................GAAACA-AGG-AAAACAGTTCGTTACATC............................................................................................................................................................................................	27	4	0.25	1	0	0	1	0	0	0	0	0	0	0	0
..............................................................................................................AGCAGAAACA-AGG-AAAACAGTTCG...................................................................................................................................................................................................	24	9	0.22	2	0	1	1	0	0	0	0	0	0	0	0
...............................................................................................................GCAGAAACA-AGG-AAAACAGTTCG...................................................................................................................................................................................................	23	9	0.22	2	0	1	0	0	0	0	1	0	0	0	0
...............................................................................................................GCAGAAACA-AGG-AAAACAGTTCGT..................................................................................................................................................................................................	24	9	0.22	2	0	1	1	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................CTCCAGCAGAGCT---------------------------------------CTGAACGGAGC.....................................................................................	24	14	0.21	3	0	2	1	0	0	0	0	0	0	0	0
.............................................................................................................................................................................TCATATCCTACTCCAGCAGAGCT---------------------------------------................................................................................................	23	15	0.20	3	0	3	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................TCCTACTCCAGCAGAGCT---------------------------------------CTGAACGG........................................................................................	26	12	0.17	2	0	1	0	1	0	0	0	0	0	0	0
..................................................................................................................................................................................TCCTACTCCAGCAGAGCT---------------------------------------CTGAACGGA.......................................................................................	27	12	0.17	2	0	2	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................TACTCCAGCAGAGCT---------------------------------------CTGAACGG........................................................................................	23	13	0.15	2	0	2	0	0	0	0	0	0	0	0	0
...........................................................................................................................G-AAAACAGTTCGTTACATCTGGCGCC.....................................................................................................................................................................................	26	7	0.14	1	0	1	0	0	0	0	0	0	0	0	0
........................................................................................................................................................................................TCCAGCAGAGCT---------------------------------------CTGAACGGAG......................................................................................	22	15	0.13	2	0	1	0	0	0	0	1	0	0	0	0
...............................................................................................TGCATTT-CGTAGCGAGCAGAAACA-AGG-A.............................................................................................................................................................................................................	28	15	0.13	2	0	1	0	0	0	0	0	0	0	0	1
................................................................................................GCATTT-CGTAGCGAGCAGAAACA-AGG-..............................................................................................................................................................................................................	26	15	0.13	2	0	0	0	0	0	0	0	0	0	0	2
........................................................................................................................................................................GCGGGTCATATCCTACTCCAGCAGAGC........................................................................................................................................	27	16	0.13	2	0	2	0	0	0	0	0	0	0	0	0
........................................................................................................................................................................GCGGGTCATATCCTACTCCAG..............................................................................................................................................	21	16	0.13	2	0	2	0	0	0	0	0	0	0	0	0
.............................................................................................................................................................................TCATATCCTACTCCAGCAGAGC........................................................................................................................................	22	17	0.12	2	0	1	0	0	0	0	1	0	0	0	0
...............................................................................................................GCAGAAACA-AGG-AAAACAGTTCGTC.................................................................................................................................................................................................	25	9	0.11	1	0	0	1	0	0	0	0	0	0	0	0
........................................................................................................GTAGCGAGCAGAAACA-AGG-AAAACAGT......................................................................................................................................................................................................	27	9	0.11	1	0	1	0	0	0	0	0	0	0	0	0
..................................................................................................................GAAACA-AGG-AAAACAGTTCGTTAC...............................................................................................................................................................................................	24	9	0.11	1	0	0	1	0	0	0	0	0	0	0	0
..............................................................................................................AGCAGAAACA-AGG-AAAACAGTTCGTT.................................................................................................................................................................................................	26	9	0.11	1	0	0	0	0	0	0	1	0	0	0	0
...............................................................................................................GCAGAAACA-AGG-AAAACAGTTCGTTA................................................................................................................................................................................................	26	9	0.11	1	0	0	0	0	0	0	1	0	0	0	0
...............................................................................................................GCAGAAACA-AGG-AAAACAGTTCGTTACA..............................................................................................................................................................................................	28	9	0.11	1	0	1	0	0	0	0	0	0	0	0	0
............................................................................................................CGAGCAGAAACA-AGG-AAAACAGTTC....................................................................................................................................................................................................	25	10	0.10	1	0	1	0	0	0	0	0	0	0	0	0
...............................................................................TCTCTGAGCTAGCCGTTGCATTT-C...................................................................................................................................................................................................................................	24	20	0.10	2	0	2	0	0	0	0	0	0	0	0	0
................................................................................................................CAGAAACA-AGG-AAAACAGTTC....................................................................................................................................................................................................	21	10	0.10	1	0	1	0	0	0	0	0	0	0	0	0
.............................................................................................................GAGCAGAAACA-AGG-AAAACAGT......................................................................................................................................................................................................	22	10	0.10	1	0	1	0	0	0	0	0	0	0	0	0
..............................................................................................................AGCAGAAACA-AGG-AAAACAGTTC....................................................................................................................................................................................................	23	10	0.10	1	0	0	0	0	0	0	1	0	0	0	0
...........................................................................................................GCGAGCAGAAACA-AGG-AAAACAGT......................................................................................................................................................................................................	24	10	0.10	1	0	0	1	0	0	0	0	0	0	0	0
...............................................................................................................GCAGAAACA-AGG-AAAACAGTTC....................................................................................................................................................................................................	22	10	0.10	1	0	0	0	1	0	0	0	0	0	0	0
...........................................................................................................GCGAGCAGAAACA-AGG-AAAA..........................................................................................................................................................................................................	20	11	0.09	1	0	1	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................CCTACTCCAGCAGAGCT---------------------------------------CTGAACGGAG......................................................................................	27	11	0.09	1	0	1	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................TACTCCAGCAGAGCT---------------------------------------CTGAACGGAGC.....................................................................................	26	11	0.09	1	0	1	0	0	0	0	0	0	0	0	0
..........................................................................................................................................................................GGGTCATATCCTACTCCAGCAGAGCT---------------------------------------C...............................................................................................	27	12	0.08	1	0	1	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................CCTACTCCAGCAGAGCT---------------------------------------CTGAACGG........................................................................................	25	12	0.08	1	0	1	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................................CATATCCTACTCCAGCAGAGCT---------------------------------------CTTT............................................................................................	26	12	0.08	1	0	1	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................................CATATCCTACTCCAGCAGAGCT---------------------------------------CTG.............................................................................................	25	12	0.08	1	0	0	0	1	0	0	0	0	0	0	0
.................................................................................................................................................................................ATCCTACTCCAGCAGAGCT---------------------------------------CTG.............................................................................................	22	12	0.08	1	0	0	0	0	0	0	1	0	0	0	0
..............................................................................................................................................................................CATATCCTACTCCAGCAGAGCT---------------------------------------CT..............................................................................................	24	12	0.08	1	0	1	0	0	0	0	0	0	0	0	0
.............................................................................................................................................................................TCATATCCTACTCCAGCAGAGCT---------------------------------------CTGA............................................................................................	27	12	0.08	1	0	1	0	0	0	0	0	0	0	0	0
.........................................................................................................................................................................CGGGTCATATCCTACTCCAGCAGAGCT---------------------------------------C...............................................................................................	28	12	0.08	1	0	1	0	0	0	0	0	0	0	0	0
...............................................................................................................................................................................ATATCCTACTCCAGCAGAGCT---------------------------------------CT..............................................................................................	23	12	0.08	1	0	1	0	0	0	0	0	0	0	0	0
...................................................................................................................................................................................CCTACTCCAGCAGAGCT---------------------------------------CTGAACG.........................................................................................	24	13	0.08	1	0	1	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................TCCTACTCCAGCAGAGCT---------------------------------------CTGAACGT........................................................................................	26	13	0.08	1	0	1	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................TCCTACTCCAGCAGAGCT---------------------------------------CTGAAC..........................................................................................	24	13	0.08	1	0	0	1	0	0	0	0	0	0	0	0
..................................................................................................................................................................................TCCTACTCCAGCAGAGCT---------------------------------------CTGAACG.........................................................................................	25	13	0.08	1	0	1	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................CTCCAGCAGAGCT---------------------------------------CTGAACGGAGCGG...................................................................................	26	14	0.07	1	0	1	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................CTCCAGCAGAGCT---------------------------------------CTGAACGGAGCGGT..................................................................................	27	14	0.07	1	1	0	0	0	0	0	0	0	0	0	0
.........................................................................................................................................................................................CCAGCAGAGCT---------------------------------------CTGAACGGAGCGG...................................................................................	24	14	0.07	1	0	1	0	0	0	0	0	0	0	0	0
..........................................................................................................................................................................................CAGCAGAGCT---------------------------------------CTGAACGGAGCGG...................................................................................	23	14	0.07	1	0	0	0	0	0	0	1	0	0	0	0
.......................................................................................................................................................................................CTCCAGCAGAGCT---------------------------------------CTGAACGGAGCGTT..................................................................................	27	14	0.07	1	0	1	0	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................CTCCAGCAGAGCT---------------------------------------CTGAACGGAGCG....................................................................................	25	14	0.07	1	0	1	0	0	0	0	0	0	0	0	0
..........................................................................................................................................................................................CAGCAGAGCT---------------------------------------CTGAACGGAGC.....................................................................................	21	14	0.07	1	0	0	0	1	0	0	0	0	0	0	0
.......................................................................................................................................................................................CTCCAGCAGAGCT---------------------------------------CTGAACGGAGCGGA..................................................................................	27	14	0.07	1	0	1	0	0	0	0	0	0	0	0	0
.................................................................................................CATTT-CGTAGCGAGCAGAAACA-AGG-AA............................................................................................................................................................................................................	27	15	0.07	1	0	0	1	0	0	0	0	0	0	0	0
........................................................................................................................................................................GCGGGTCATATCCTACTCCAGCAGAGCT---------------------------------------................................................................................................	28	15	0.07	1	0	1	0	0	0	0	0	0	0	0	0
..............................................................................................TTGCATTT-CGTAGCGAGCAGAAACA-AGG-..............................................................................................................................................................................................................	28	15	0.07	1	0	0	0	0	0	0	1	0	0	0	0
..................................................................................................................................................................................TCCTACTCCAGCAGAGCT---------------------------------------................................................................................................	18	16	0.06	1	0	0	0	0	1	0	0	0	0	0	0
........................................................................................................................................................................................TCCAGCAGAGCT---------------------------------------CTGAACGG........................................................................................	20	16	0.06	1	0	1	0	0	0	0	0	0	0	0	0
........................................................................................................................................................................GCGGGTCATATCCTACTCCAGCAGA..........................................................................................................................................	25	16	0.06	1	0	1	0	0	0	0	0	0	0	0	0
........................................................................................................................................................................GCGGGTCATATCCTACTCCAGCAGAG.........................................................................................................................................	26	16	0.06	1	0	1	0	0	0	0	0	0	0	0	0
.........................................................................................................................................................................CGGGTCATATCCTACTCCAGCAGA..........................................................................................................................................	24	16	0.06	1	0	1	0	0	0	0	0	0	0	0	0
.........................................................................................................................................................................CGGGTCATATCCTACTCCAGCAGAG.........................................................................................................................................	25	16	0.06	1	0	1	0	0	0	0	0	0	0	0	0
...................................................................................................TTT-CGTAGCGAGCAGAAACA-AGG-AA............................................................................................................................................................................................................	25	17	0.06	1	0	1	0	0	0	0	0	0	0	0	0
..............................................................................................................................................................................CATATCCTACTCCAGCAGAGC........................................................................................................................................	21	17	0.06	1	0	1	0	0	0	0	0	0	0	0	0
....................................................................................................TT-CGTAGCGAGCAGAAACA-AGG-A.............................................................................................................................................................................................................	23	17	0.06	1	0	0	0	0	0	0	1	0	0	0	0
....................................................................................................TT-CGTAGCGAGCAGAAACA-AGG-AAT...........................................................................................................................................................................................................	25	17	0.06	1	0	0	0	0	0	0	1	0	0	0	0
.........................................................................................AGCCGTTGCATTT-CGTAGCGAGC..........................................................................................................................................................................................................................	23	20	0.05	1	0	0	1	0	0	0	0	0	0	0	0
.................................................................................TCTGAGCTAGCCGTTGCATTT-CGT.................................................................................................................................................................................................................................	24	20	0.05	1	0	1	0	0	0	0	0	0	0	0	0
..................................................................................................................................AGTTCGTTACATCTGGCGC......................................................................................................................................................................................	19	20	0.05	1	0	0	1	0	0	0	0	0	0	0	0
...................................................................................................................................GTTCGTTACATCTGGCGCCCA...................................................................................................................................................................................	21	20	0.05	1	0	1	0	0	0	0	0	0	0	0	0
...............................................................................................TGCATTT-CGTAGCGAGCAGAAACA-AG................................................................................................................................................................................................................	26	20	0.05	1	0	1	0	0	0	0	0	0	0	0	0
........................................................................................TAGCCGTTGCATTT-CGTAGCG.............................................................................................................................................................................................................................	21	20	0.05	1	0	0	0	0	0	0	1	0	0	0	0
................................................................................CTCTGAGCTAGCCGTTGCATTT-CGTA................................................................................................................................................................................................................................	26	20	0.05	1	1	0	0	0	0	0	0	0	0	0	0
.........................................................................................AGCCGTTGCATTT-CGTAGCGA............................................................................................................................................................................................................................	21	20	0.05	1	0	0	1	0	0	0	0	0	0	0	0
...............................................................................................................................AACAGTTCGTTACATCTGGCGCC.....................................................................................................................................................................................	23	20	0.05	1	0	1	0	0	0	0	0	0	0	0	0

dp5

AG-------------------------------------------------------------------------------------------------------------------ACGGAAC-AGAACGGTTCGTTACAACTGGCGCCCAA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
.................................................................................................................................CGGTTCGTTACAACTGGCGCC.....................................................................................................................................................................................	21	13	0.38	5	0	5	0	0	0	0	0	0	0	0
.................................................................................................................................CGGTTCGTTACAACTGGCGC......................................................................................................................................................................................	20	13	0.38	5	0	5	0	0	0	0	0	0	0	0
AG-------------------------------------------------------------------------------------------------------------------ACGGAAC-AGAACGGTTCGT..................................................................................................................................................................................................	21	3	0.33	1	0	1	0	0	0	0	0	0	0	0
AG-------------------------------------------------------------------------------------------------------------------ACGGAAC-AGAACGGTTT....................................................................................................................................................................................................	19	4	0.25	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................CGGTTCGTTACAACTGGCGCCC....................................................................................................................................................................................	22	13	0.15	2	0	2	0	0	0	0	0	0	0	0
.................................................................................................................................CGGTTCGTTACAACTGGCG.......................................................................................................................................................................................	19	13	0.15	2	0	2	0	0	0	0	0	0	0	0
.................................................................................................................................CGGTTCGTTACAACTGGCGCCCAA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	24	12	0.08	1	0	1	0	0	0	0	0	0	0	0
.............................................................................................................................AGAACGGTTCGTTACAACTGGCG.......................................................................................................................................................................................	23	12	0.08	1	0	1	0	0	0	0	0	0	0	0
.................................................................................................................................CGGTTCGTTACAACTGGCGCCA....................................................................................................................................................................................	22	13	0.08	1	0	1	0	0	0	0	0	0	0	0
....................................................................................................................................TTCGTTACAACTGGCGCCCA...................................................................................................................................................................................	20	20	0.05	1	0	1	0	0	0	0	0	0	0	0
..................................................................................................................................GGTTCGTTACAACTGGCGC......................................................................................................................................................................................	19	20	0.05	1	0	1	0	0	0	0	0	0	0	0
...................................................................................................................................GTTCGTTACAACTGGCGCCC....................................................................................................................................................................................	20	20	0.05	1	0	1	0	0	0	0	0	0	0	0
..................................................................................................................................GGTTCGTTACAACTGGCGCCCA...................................................................................................................................................................................	22	20	0.05	1	0	1	0	0	0	0	0	0	0	0
...................................................................................................................................GTTCGTTACAACTGGCGCCCA...................................................................................................................................................................................	21	20	0.05	1	0	1	0	0	0	0	0	0	0	0
...................................................................................................................................GTTCGTTACAACTGGCGA......................................................................................................................................................................................	18	20	0.05	1	0	1	0	0	0	0	0	0	0	0

droPer2

AG-------------------------------------------------------------------------------------------------------------------ACGGAGA-AGAACGGTTCGTTACAACTGGCGCCCAA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
.....................................................................................................................ACGGAGA-AGAACGGTTCGTTACAA.............................................................................................................................................................................................	24	20	0.50	10	0	10	0	0	0	0
.....................................................................................................................ACGGAGA-AGAACGGTTCGTTACAAC............................................................................................................................................................................................	25	15	0.47	7	0	7	0	0	0	0
......................................................................................................................CGGAGA-AGAACGGTTCGTTACAACTG..........................................................................................................................................................................................	26	14	0.21	3	3	0	0	0	0	0
.....................................................................................................................ACGGAGA-AGAACGGTTCGTTACA..............................................................................................................................................................................................	23	20	0.20	4	0	4	0	0	0	0
.....................................................................................................................ACGGAGA-AGAACGGTTCGTTACAACT...........................................................................................................................................................................................	26	15	0.13	2	0	2	0	0	0	0
......................................................................................................................CGGAGA-AGAACGGTTCGTTAC...............................................................................................................................................................................................	21	20	0.10	2	0	2	0	0	0	0
...........................................................................................................................A-AGAACGGTTCGTTACAACTGGCGC......................................................................................................................................................................................	25	15	0.07	1	0	1	0	0	0	0
.................................................................................................................................CGGTTCGTTACAACTGGCGC......................................................................................................................................................................................	20	20	0.05	1	0	1	0	0	0	0
......................................................................................................................CGGAGA-AGAACGGTTCGTTACA..............................................................................................................................................................................................	22	20	0.05	1	0	1	0	0	0	0
................................................................................................................................ACGGTTCGTTACAACTGGCGCC.....................................................................................................................................................................................	22	20	0.05	1	0	1	0	0	0	0
......................................................................................................................CGGAGA-AGAACGGTTCGT..................................................................................................................................................................................................	18	20	0.05	1	0	1	0	0	0	0
.G-------------------------------------------------------------------------------------------------------------------ACGGAGA-AGAACGGTTCGTTA................................................................................................................................................................................................	22	20	0.05	1	0	1	0	0	0	0
....................................................................................................................................TTCGTTACAACTGGCGCCCAA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	21	20	0.05	1	0	1	0	0	0	0
.....................................................................................................................ACGGAGA-AGAACGGTTCGTTAC...............................................................................................................................................................................................	22	20	0.05	1	0	0	1	0	0	0

droWil2

AA----------------------------------------------------------------------------CAA-CTGCAG------------------------CAGCAACA-ACA-ACAGCAGCA-ACAACAACAGCAGCAGCA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
................................................................................................................................GCAGCA-ACAACAACAGCA........................................................................................................................................................................................	18	20	0.15	3	0	0	0	3	0
..................................................................................................................GCAACA-ACA-ACAGCAGCA-ACA.................................................................................................................................................................................................	21	20	0.10	2	0	0	0	2	0
...................................................................................TGCAG------------------------CAGCAACA-ACA-ACAGCAGCA-ACA.................................................................................................................................................................................................	28	14	0.07	1	0	0	0	1	0
AA----------------------------------------------------------------------------CAA-CTGCAG------------------------CAGCAACA-ACA-ACA...........................................................................................................................................................................................................	25	15	0.07	1	0	1	0	0	0
...............................................................................................................................AGCAGCA-ACAACAACAGCAG.......................................................................................................................................................................................	20	20	0.05	1	0	0	0	1	0
.............................................................................................................................ACAGCAGCA-ACAACAACAGCA........................................................................................................................................................................................	21	20	0.05	1	0	0	0	0	1
..........................................................................................................................CA-ACAGCAGCA-ACAACAACAGCAGC......................................................................................................................................................................................	25	20	0.05	1	0	0	0	1	0

droMoj3

AG--------------------------------------------------------------------------------------------------------------CAACAACA-GCA-GCAGCAGG-------CCCAGAAGTACAG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
................................................................................................................CAACAACA-GCA-GCAGCAGG-------CC.............................................................................................................................................................................................	21	20	1.05	21	21	0	0	0	0	0
..................................................................................................................ACAACA-GCA-GCAGCAGG-------CCCA...........................................................................................................................................................................................	21	20	0.50	10	10	0	0	0	0	0
.................................................................................................................AACAACA-GCA-GCAGCAGG-------CCC............................................................................................................................................................................................	21	20	0.35	7	7	0	0	0	0	0
AG--------------------------------------------------------------------------------------------------------------CAACAACA-GCA-GCAGCAGG-------...............................................................................................................................................................................................	21	20	0.25	5	5	0	0	0	0	0
.................................................................................................................AACAACA-GCA-GCAGCAGG-------CC.............................................................................................................................................................................................	20	20	0.20	4	4	0	0	0	0	0
...................................................................................................................CAACA-GCA-GCAGCAGG-------CCCA...........................................................................................................................................................................................	20	20	0.15	3	3	0	0	0	0	0
.................................................................................................................AACAACA-GCA-GCAGCAGG-------C..............................................................................................................................................................................................	19	20	0.10	2	2	0	0	0	0	0
..................................................................................................................ACAACA-GCA-GCAGCAGG-------CCC............................................................................................................................................................................................	20	20	0.10	2	1	0	0	0	1	0
...................................................................................................................CAACA-GCA-GCAGCAGG-------CCC............................................................................................................................................................................................	19	20	0.05	1	1	0	0	0	0	0
.................................................................................................................AACAACA-GCA-GCAGCAGG-------CCCT...........................................................................................................................................................................................	22	20	0.05	1	0	0	1	0	0	0
..................................................................................................................ACAACA-GCA-GCAGCAGG-------CCCT...........................................................................................................................................................................................	21	20	0.05	1	1	0	0	0	0	0
.........................................................................................................................GCA-GCAGCAGG-------CCCAGAAGA......................................................................................................................................................................................	20	20	0.05	1	1	0	0	0	0	0
.G--------------------------------------------------------------------------------------------------------------CAACAACA-GCA-GCAGCAGG-------...............................................................................................................................................................................................	20	20	0.05	1	1	0	0	0	0	0
.G--------------------------------------------------------------------------------------------------------------CAACAACA-GCA-GCAGCAGG-------CC.............................................................................................................................................................................................	22	20	0.05	1	1	0	0	0	0	0

dan_3375

scaffold_13499:1582877-1583027 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-25.0	-25.0	-25.0