dan_3280

##################################################--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- CGTAAGCAGTACGCTAAGACCCTCCTGGGCCTGAATGATCAGTGTTTGAAGTACGTGGAGTGGCACGGCTATAATGAAGCATTCTATCCAACCGAAAAGCCCCGAAGTCGTCGCGATACTTTGGGACAATCGGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN ***********************************************************************...................(((.....((((((((((...)))..)))))))....)))*********************************************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M044 female body	V106 head	V105 male body	V055 head	V039 embryo	M058 embryo
...............................................................................................................CGCGATACTTTGGGACAATCG.......................................................................................................................	21	0	2	11.00	22	13	7	1	1	0	0
..............................................................................................................TCGCGATACTTTGGGACAATC........................................................................................................................	21	0	1	8.00	8	7	0	1	0	0	0
..........................................................................................ACCGAAAAGCCCCGAAGTCGT............................................................................................................................................	21	0	1	8.00	8	7	0	1	0	0	0
...........................................................................................................TCGTCGCGATACTTTGGGACA...........................................................................................................................	21	0	1	5.00	5	4	0	0	1	0	0
.........................................................................ATGAAGCATTCTATCCAACCG.............................................................................................................................................................	21	0	1	4.00	4	4	0	0	0	0	0
.........................................................................................................AGTCGTCGCGATACTTTGG...............................................................................................................................	19	0	1	4.00	4	3	0	0	1	0	0
...............................................GAAGTACGTGGAGTGGCACGG.......................................................................................................................................................................................	21	0	2	4.00	8	3	2	3	0	0	0
...........................................GTTTGAAGTACGTGGAGTGGC...........................................................................................................................................................................................	21	0	1	3.00	3	1	1	0	1	0	0
...............................................................................................AAAGCCCCGAAGTCGTCGCGA.......................................................................................................................................	21	0	1	3.00	3	0	1	1	1	0	0
....................................................................................................CCCGAAGTCGTCGCGATACTT..................................................................................................................................	21	0	1	3.00	3	3	0	0	0	0	0
............................................TTTGAAGTACGTGGAGTGG............................................................................................................................................................................................	19	0	1	3.00	3	1	0	2	0	0	0
.........................................................................................................AGTCGTCGCGATACTTTGGGA.............................................................................................................................	21	0	1	3.00	3	1	1	1	0	0	0
....................................................................................TATCCAACCGAAAAGCCCCGA..................................................................................................................................................	21	0	1	3.00	3	2	0	1	0	0	0
............................................................................................................CGTCGCGATACTTTGGGACA...........................................................................................................................	20	0	1	3.00	3	3	0	0	0	0	0
........................................AGTGTTTGAAGTACGTGGAGT..............................................................................................................................................................................................	21	0	1	2.00	2	1	1	0	0	0	0
................................GAATGATCAGTGTTTGAAGTA......................................................................................................................................................................................................	21	0	1	2.00	2	0	2	0	0	0	0
..............................................................................................AAAAGCCCCGAAGTCGTCGCG........................................................................................................................................	21	0	1	2.00	2	2	0	0	0	0	0
.....................................................................TATAATGAAGCATTCTATCCA.................................................................................................................................................................	21	0	1	2.00	2	1	0	1	0	0	0
............................................TTTGAAGTACGTGGAGTGGC...........................................................................................................................................................................................	20	0	1	2.00	2	2	0	0	0	0	0
..............................................................................................................TCGCGATACTTTGGGACAAT.........................................................................................................................	20	0	1	2.00	2	1	1	0	0	0	0
.......................................................................................................GAAGTCGTCGCGATACTTTGG...............................................................................................................................	21	0	1	2.00	2	2	0	0	0	0	0
........................CTGGGCCTGAATGATCAGTGT..............................................................................................................................................................................................................	21	0	1	2.00	2	0	2	0	0	0	0
.................................................................................................AGCCCCGAAGTCGTCGCGATA.....................................................................................................................................	21	0	1	2.00	2	0	2	0	0	0	0
..................................................GTACGTGGAGTGGCACGGCTA....................................................................................................................................................................................	21	0	2	1.50	3	1	2	0	0	0	0
..................................................................................................................GATACTTTGGGACAATCGGAG....................................................................................................................	21	0	2	1.50	3	2	0	1	0	0	0
...........................................................................GAAGCATTCTATCCAACCGAA...........................................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0
..........................................TGTTTGAAGTACGTGGAGTGG............................................................................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0
...........CGCTAAGACCCTCCTGGGCCTGAAT.......................................................................................................................................................................................................................	25	0	1	1.00	1	0	1	0	0	0	0
..........................................................................TGAAGCATTCTATCCAACCGA............................................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0
............................GCCTGAATGATCAGTGTTTGA..........................................................................................................................................................................................................	21	0	1	1.00	1	0	1	0	0	0	0
.....................................................CGTGGAGTGGCACGGCTATAA.................................................................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0
.......................................................................TAATGAAGCATTCTATCCAAC...............................................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0
..........................................................................................................GTCGTCGCTATACTTTGGGA.............................................................................................................................	20	1	1	1.00	1	1	0	0	0	0	0
..........................................................................................................GTCGTCGCGATACTTTGGGAC............................................................................................................................	21	0	1	1.00	1	0	0	1	0	0	0
.........................................................................................AACCGAAAAGCCCCGAAGTCG.............................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0
...........................................GTTTGAAGTACGTGGAGTGTC...........................................................................................................................................................................................	21	1	1	1.00	1	0	1	0	0	0	0
...........................................................................................................TCGTCGCGATACTTTGGGAC............................................................................................................................	20	0	1	1.00	1	1	0	0	0	0	0
...................................TGATCAGTGTTTGAAGTACGT...................................................................................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0
.................................................................................................................CGATACTTTGGGACAATCGGA.....................................................................................................................	21	0	2	1.00	2	2	0	0	0	0	0
.........................................GTGTTTGAAGTACGTGGAGTGG............................................................................................................................................................................................	22	0	1	1.00	1	1	0	0	0	0	0
..........................................................................................ACCGAAAAGCCCCGAAGTCG.............................................................................................................................................	20	0	1	1.00	1	1	0	0	0	0	0
.........................................................................................................AGTCGTCGCGATACTTTGGG..............................................................................................................................	20	0	1	1.00	1	1	0	0	0	0	0
..........................................................................TGAAGCATTCTATCCAACCG.............................................................................................................................................................	20	0	1	1.00	1	0	0	1	0	0	0
...............................................................................................................CGCGATACTTTGGGACAATC........................................................................................................................	20	0	2	1.00	2	2	0	0	0	0	0
.................................AATGATCAGTGTTTGAAGTAC.....................................................................................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0
................................GAATGATCAGTGTTTGAAGTACGTG..................................................................................................................................................................................................	25	0	1	1.00	1	0	1	0	0	0	0
...........................................................................................CCGAAAAGCCCCGAAGTCGTC...........................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0
..................................................................................TCTATCCAACCGAAAAGCCCC....................................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0
.............................................................................................................GTCGCGATACTTTTGGACA...........................................................................................................................	19	1	1	1.00	1	0	0	0	1	0	0
.................................AATGATCAGTGTTTGAAGT.......................................................................................................................................................................................................	19	0	1	1.00	1	0	0	1	0	0	0
........................................................................AATGAAGCATTCTATCCAACC..............................................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0
..........................................................................................................GTCGTCGCGATACTTTGGGA.............................................................................................................................	20	0	1	1.00	1	1	0	0	0	0	0
....................................................................................................CCCGAAGTTGTCGCGATACTT..................................................................................................................................	21	1	1	1.00	1	0	1	0	0	0	0
...........................................GTTTGAAGTACGTGGAGTGG............................................................................................................................................................................................	20	0	1	1.00	1	1	0	0	0	0	0
.........................................................................ATGAAGCATTCTATCCAACC..............................................................................................................................................................	20	0	1	1.00	1	1	0	0	0	0	0
................................................................................................AAGCCCCGAAGTCGTCGCGAT......................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0
.................................................................................................AGCCCCGAAGTCGTCGCGATAC....................................................................................................................................	22	0	1	1.00	1	1	0	0	0	0	0
......................................TCAGTGTTTGAAGTACGTGGAG...............................................................................................................................................................................................	22	0	1	1.00	1	1	0	0	0	0	0
...................................................................................CTATCCAACCGAAAAGCCCCG...................................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0
.................................................................................................AGCCCCGAAGTCGTCGCGAT......................................................................................................................................	20	0	1	1.00	1	1	0	0	0	0	0
...........................................................................................CCGAAAAGCCCCGAAGTCG.............................................................................................................................................	19	0	1	1.00	1	1	0	0	0	0	0
.............................................TTGAAGTACGTGGAGTGGCAC.........................................................................................................................................................................................	21	0	2	1.00	2	2	0	0	0	0	0
...........................................................................................CCGAAAAGCCCCGAAGTCGTCG..........................................................................................................................................	22	0	1	1.00	1	1	0	0	0	0	0
..................................................................................................GCCCCGAAGTCGTCGCGATA.....................................................................................................................................	20	0	1	1.00	1	1	0	0	0	0	0
............................................TTTGAAGTGCGTGGAGTGGC...........................................................................................................................................................................................	20	1	1	1.00	1	1	0	0	0	0	0
......................................................................................TCCAACCGAAAAGCCCCGAAGT...............................................................................................................................................	22	0	1	1.00	1	1	0	0	0	0	0
..............................................................................................................TCGCGATACTTTGGGACAATCG.......................................................................................................................	22	0	1	1.00	1	0	0	1	0	0	0
.........................TGGTCCTGAATGATCAGTGTT.............................................................................................................................................................................................................	21	1	1	1.00	1	0	1	0	0	0	0
..............................................................................................AAAAGCCCCGAAGTCGCCGC.........................................................................................................................................	20	1	1	1.00	1	1	0	0	0	0	0
...............................................................CACGGCTATAATGAAGCATTC.......................................................................................................................................................................	21	0	1	1.00	1	0	0	0	1	0	0
...........................................................................GAAGCATTCTATCCAACCGA............................................................................................................................................................	20	0	1	1.00	1	0	1	0	0	0	0
................................................................................................AAGCCCCGAAGTCGTCGC.........................................................................................................................................	18	0	1	1.00	1	1	0	0	0	0	0
.................................................AGTACGTGGAGTGGCACGG.......................................................................................................................................................................................	19	0	2	1.00	2	2	0	0	0	0	0
...........................................................................................CCGAAAAGCCCCGAAGTCGT............................................................................................................................................	20	0	1	1.00	1	1	0	0	0	0	0
..................................ATGATCAGTGTTTGAAGTACG....................................................................................................................................................................................................	21	0	1	1.00	1	0	0	1	0	0	0
.........................................................GAGTGGCAGGGCTATAATGA..............................................................................................................................................................................	20	1	1	1.00	1	1	0	0	0	0	0
...................................................TACGTGGAGTGGCACGGC......................................................................................................................................................................................	18	0	2	0.50	1	1	0	0	0	0	0
...............................................GAAGTACGTGGAGTGGCACGGC......................................................................................................................................................................................	22	0	2	0.50	1	0	1	0	0	0	0
...............................................................................................................CGCGATACTTTTGGACAATCG.......................................................................................................................	21	1	2	0.50	1	0	1	0	0	0	0
...............................................................................................................CGCGATACTTTGGGGCAATCG.......................................................................................................................	21	1	2	0.50	1	0	1	0	0	0	0
................................................................................................................GCGATACTTTGGGACAATCGG......................................................................................................................	21	0	2	0.50	1	0	0	1	0	0	0
.................................................AGTACGTGGAGTGGCACGGC......................................................................................................................................................................................	20	0	2	0.50	1	1	0	0	0	0	0
.................................................................................................................CGATACTTAGGGACAATCGGA.....................................................................................................................	21	1	2	0.50	1	1	0	0	0	0	0
.............................................TTGAAGTACGTGGAGTGGCACGG.......................................................................................................................................................................................	23	0	2	0.50	1	1	0	0	0	0	0
...............................................GAAGTACGTGGAGTGGCACGGTTT....................................................................................................................................................................................	24	2	2	0.50	1	0	0	0	1	0	0
...............................................GAAGTACGTGGAGTGGCACG........................................................................................................................................................................................	20	0	2	0.50	1	1	0	0	0	0	0
...........................................ATTTGAAGGACGTGGAGTGGCA..........................................................................................................................................................................................	22	2	2	0.50	1	1	0	0	0	0	0
........................................................GGAGTGGCAGGGCTAGAAT................................................................................................................................................................................	19	2	2	0.50	1	0	0	0	0	1	0
...............................................................................................................CGCGATACTTTGGGACAACCG.......................................................................................................................	21	1	2	0.50	1	0	0	0	1	0	0
...............................................................................................................CGCGATACTTTGGGACAATCGC......................................................................................................................	22	1	2	0.50	1	1	0	0	0	0	0
..............................................TGAAGTACGTGGAGTGGCTT.........................................................................................................................................................................................	20	2	2	0.50	1	0	1	0	0	0	0
...............................TGAATGCTAAGTGTTTGAGG........................................................................................................................................................................................................	20	3	13	0.15	2	0	0	2	0	0	0
................................GAATGATAAGTGTTGGAGG........................................................................................................................................................................................................	19	3	20	0.15	3	0	0	3	0	0	0
.............ATAAGACCCTCTTGAGCCTGA.........................................................................................................................................................................................................................	21	3	19	0.05	1	0	0	0	0	0	1

##################################################---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
CGTAAGCAGTACGCTAAGACCCTCCTGGGCCTGAATGATCAGTGTTTGAAGTACGTGGAGTGGCACGGCTATAATGAAGCATTCTATCCAACCGAAAAGCCCCGAAGTCGTCGCGATACTTTGGGACAATCGGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
*************************************************************************...................(((.....((((((((((...)))..)))))))....)))***********************************************************************************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M044
female body

V106
head

V105
male body

V055
head

V039
embryo

M058
embryo

...............................................................................................................CGCGATACTTTGGGACAATCG.......................................................................................................................

11.00

..............................................................................................................TCGCGATACTTTGGGACAATC........................................................................................................................

8.00

..........................................................................................ACCGAAAAGCCCCGAAGTCGT............................................................................................................................................

8.00

...........................................................................................................TCGTCGCGATACTTTGGGACA...........................................................................................................................

5.00

.........................................................................ATGAAGCATTCTATCCAACCG.............................................................................................................................................................

4.00

.........................................................................................................AGTCGTCGCGATACTTTGG...............................................................................................................................

4.00

...............................................GAAGTACGTGGAGTGGCACGG.......................................................................................................................................................................................

4.00

...........................................GTTTGAAGTACGTGGAGTGGC...........................................................................................................................................................................................

3.00

...............................................................................................AAAGCCCCGAAGTCGTCGCGA.......................................................................................................................................

3.00

....................................................................................................CCCGAAGTCGTCGCGATACTT..................................................................................................................................

3.00

............................................TTTGAAGTACGTGGAGTGG............................................................................................................................................................................................

3.00

.........................................................................................................AGTCGTCGCGATACTTTGGGA.............................................................................................................................

3.00

....................................................................................TATCCAACCGAAAAGCCCCGA..................................................................................................................................................

3.00

............................................................................................................CGTCGCGATACTTTGGGACA...........................................................................................................................

3.00

........................................AGTGTTTGAAGTACGTGGAGT..............................................................................................................................................................................................

2.00

................................GAATGATCAGTGTTTGAAGTA......................................................................................................................................................................................................

2.00

..............................................................................................AAAAGCCCCGAAGTCGTCGCG........................................................................................................................................

2.00

.....................................................................TATAATGAAGCATTCTATCCA.................................................................................................................................................................

2.00

............................................TTTGAAGTACGTGGAGTGGC...........................................................................................................................................................................................

2.00

..............................................................................................................TCGCGATACTTTGGGACAAT.........................................................................................................................

2.00

.......................................................................................................GAAGTCGTCGCGATACTTTGG...............................................................................................................................

2.00

........................CTGGGCCTGAATGATCAGTGT..............................................................................................................................................................................................................

2.00

.................................................................................................AGCCCCGAAGTCGTCGCGATA.....................................................................................................................................

2.00

..................................................GTACGTGGAGTGGCACGGCTA....................................................................................................................................................................................

1.50

..................................................................................................................GATACTTTGGGACAATCGGAG....................................................................................................................

1.50

...........................................................................GAAGCATTCTATCCAACCGAA...........................................................................................................................................................

1.00

..........................................TGTTTGAAGTACGTGGAGTGG............................................................................................................................................................................................

1.00

...........CGCTAAGACCCTCCTGGGCCTGAAT.......................................................................................................................................................................................................................

1.00

..........................................................................TGAAGCATTCTATCCAACCGA............................................................................................................................................................

1.00

............................GCCTGAATGATCAGTGTTTGA..........................................................................................................................................................................................................

1.00

.....................................................CGTGGAGTGGCACGGCTATAA.................................................................................................................................................................................

1.00

.......................................................................TAATGAAGCATTCTATCCAAC...............................................................................................................................................................

1.00

..........................................................................................................GTCGTCGCTATACTTTGGGA.............................................................................................................................

1.00

..........................................................................................................GTCGTCGCGATACTTTGGGAC............................................................................................................................

1.00

.........................................................................................AACCGAAAAGCCCCGAAGTCG.............................................................................................................................................

1.00

...........................................GTTTGAAGTACGTGGAGTGTC...........................................................................................................................................................................................

1.00

...........................................................................................................TCGTCGCGATACTTTGGGAC............................................................................................................................

1.00

...................................TGATCAGTGTTTGAAGTACGT...................................................................................................................................................................................................

1.00

.................................................................................................................CGATACTTTGGGACAATCGGA.....................................................................................................................

1.00

.........................................GTGTTTGAAGTACGTGGAGTGG............................................................................................................................................................................................

1.00

..........................................................................................ACCGAAAAGCCCCGAAGTCG.............................................................................................................................................

1.00

.........................................................................................................AGTCGTCGCGATACTTTGGG..............................................................................................................................

1.00

..........................................................................TGAAGCATTCTATCCAACCG.............................................................................................................................................................

1.00

...............................................................................................................CGCGATACTTTGGGACAATC........................................................................................................................

1.00

.................................AATGATCAGTGTTTGAAGTAC.....................................................................................................................................................................................................

1.00

................................GAATGATCAGTGTTTGAAGTACGTG..................................................................................................................................................................................................

1.00

...........................................................................................CCGAAAAGCCCCGAAGTCGTC...........................................................................................................................................

1.00

..................................................................................TCTATCCAACCGAAAAGCCCC....................................................................................................................................................

1.00

.............................................................................................................GTCGCGATACTTTTGGACA...........................................................................................................................

1.00

.................................AATGATCAGTGTTTGAAGT.......................................................................................................................................................................................................

1.00

........................................................................AATGAAGCATTCTATCCAACC..............................................................................................................................................................

1.00

..........................................................................................................GTCGTCGCGATACTTTGGGA.............................................................................................................................

1.00

....................................................................................................CCCGAAGTTGTCGCGATACTT..................................................................................................................................

1.00

...........................................GTTTGAAGTACGTGGAGTGG............................................................................................................................................................................................

1.00

.........................................................................ATGAAGCATTCTATCCAACC..............................................................................................................................................................

1.00

................................................................................................AAGCCCCGAAGTCGTCGCGAT......................................................................................................................................

1.00

.................................................................................................AGCCCCGAAGTCGTCGCGATAC....................................................................................................................................

1.00

......................................TCAGTGTTTGAAGTACGTGGAG...............................................................................................................................................................................................

1.00

...................................................................................CTATCCAACCGAAAAGCCCCG...................................................................................................................................................

1.00

.................................................................................................AGCCCCGAAGTCGTCGCGAT......................................................................................................................................

1.00

...........................................................................................CCGAAAAGCCCCGAAGTCG.............................................................................................................................................

1.00

.............................................TTGAAGTACGTGGAGTGGCAC.........................................................................................................................................................................................

1.00

...........................................................................................CCGAAAAGCCCCGAAGTCGTCG..........................................................................................................................................

1.00

..................................................................................................GCCCCGAAGTCGTCGCGATA.....................................................................................................................................

1.00

............................................TTTGAAGTGCGTGGAGTGGC...........................................................................................................................................................................................

1.00

......................................................................................TCCAACCGAAAAGCCCCGAAGT...............................................................................................................................................

1.00

..............................................................................................................TCGCGATACTTTGGGACAATCG.......................................................................................................................

1.00

.........................TGGTCCTGAATGATCAGTGTT.............................................................................................................................................................................................................

1.00

..............................................................................................AAAAGCCCCGAAGTCGCCGC.........................................................................................................................................

1.00

...............................................................CACGGCTATAATGAAGCATTC.......................................................................................................................................................................

1.00

...........................................................................GAAGCATTCTATCCAACCGA............................................................................................................................................................

1.00

................................................................................................AAGCCCCGAAGTCGTCGC.........................................................................................................................................

1.00

.................................................AGTACGTGGAGTGGCACGG.......................................................................................................................................................................................

1.00

...........................................................................................CCGAAAAGCCCCGAAGTCGT............................................................................................................................................

1.00

..................................ATGATCAGTGTTTGAAGTACG....................................................................................................................................................................................................

1.00

.........................................................GAGTGGCAGGGCTATAATGA..............................................................................................................................................................................

1.00

...................................................TACGTGGAGTGGCACGGC......................................................................................................................................................................................

0.50

...............................................GAAGTACGTGGAGTGGCACGGC......................................................................................................................................................................................

0.50

...............................................................................................................CGCGATACTTTTGGACAATCG.......................................................................................................................

0.50

...............................................................................................................CGCGATACTTTGGGGCAATCG.......................................................................................................................

0.50

................................................................................................................GCGATACTTTGGGACAATCGG......................................................................................................................

0.50

.................................................AGTACGTGGAGTGGCACGGC......................................................................................................................................................................................

0.50

.................................................................................................................CGATACTTAGGGACAATCGGA.....................................................................................................................

0.50

.............................................TTGAAGTACGTGGAGTGGCACGG.......................................................................................................................................................................................

0.50

...............................................GAAGTACGTGGAGTGGCACGGTTT....................................................................................................................................................................................

0.50

...............................................GAAGTACGTGGAGTGGCACG........................................................................................................................................................................................

0.50

...........................................ATTTGAAGGACGTGGAGTGGCA..........................................................................................................................................................................................

0.50

........................................................GGAGTGGCAGGGCTAGAAT................................................................................................................................................................................

0.50

...............................................................................................................CGCGATACTTTGGGACAACCG.......................................................................................................................

0.50

...............................................................................................................CGCGATACTTTGGGACAATCGC......................................................................................................................

0.50

..............................................TGAAGTACGTGGAGTGGCTT.........................................................................................................................................................................................

0.50

...............................TGAATGCTAAGTGTTTGAGG........................................................................................................................................................................................................

0.15

................................GAATGATAAGTGTTGGAGG........................................................................................................................................................................................................

0.15

.............ATAAGACCCTCTTGAGCCTGA.........................................................................................................................................................................................................................

0.05

GCATTCGTCATGCGATTCTGGGAGGACCCGGACTTACTAGTCACAAACTTCATGCACCTCACCGTGCCGATATTACTTCGTAAGATAGGTTGGCTTTTCGGGGCTTCAGCAGCGCTATGAAACCCTGTTAGCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN *********************************************************************************************************************...................(((.....((((((((((...)))..)))))))....)))***********************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M044 female body	V055 head	V105 male body
...............................................................................................................GCGCTATGAAACCCTGTTAGC.......................................................................................................................	21	0	2	2.00	4	4	0	0
................................................................................................................CGCTATGAAACCCTGTTAGCC......................................................................................................................	21	0	2	1.50	3	3	0	0
....................................CTCGTCACAAACTTCACGCAA..................................................................................................................................................................................................	21	3	2	0.50	1	0	1	0
................................................................................................................CGCTATGAAACCGTGTTAGCC......................................................................................................................	21	1	2	0.50	1	1	0	0
.................................................TCATGCACCTCACCGTGCCGA.....................................................................................................................................................................................	21	0	2	0.50	1	1	0	0
..................................................................................................................CTATGAAACCCTGTTAGCCTC....................................................................................................................	21	0	2	0.50	1	0	1	0
..................................................CATGCACCTCACCGTGCCGAT....................................................................................................................................................................................	21	0	2	0.50	1	1	0	0
................................................................................................................CGCTATGAAACCCTGTTAGC.......................................................................................................................	20	0	2	0.50	1	1	0	0
...............................................................................................................GCGCTATGAAACACTGTCAGA.......................................................................................................................	21	3	2	0.50	1	0	1	0
.................................................................................................................GCTATGAAACCCTGTTAGC.......................................................................................................................	19	0	2	0.50	1	1	0	0
.................................................................................................................GCTATGAAACCCTGTTAGCCT.....................................................................................................................	21	0	2	0.50	1	1	0	0
...............................................................................................................GCGCTATGAAACCCCGTTAGC.......................................................................................................................	21	1	2	0.50	1	1	0	0
..................................................................................AGATAAGTTGGCCTTTCGCGG....................................................................................................................................................	21	3	3	0.33	1	0	1	0
......................................................................................AGTTTGGCCTATCGGGGCTT.................................................................................................................................................	20	3	10	0.10	1	0	0	1

GCATTCGTCATGCGATTCTGGGAGGACCCGGACTTACTAGTCACAAACTTCATGCACCTCACCGTGCCGATATTACTTCGTAAGATAGGTTGGCTTTTCGGGGCTTCAGCAGCGCTATGAAACCCTGTTAGCCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
***********************************************************************************************************************...................(((.....((((((((((...)))..)))))))....)))*************************************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M044
female body

V055
head

V105
male body

...............................................................................................................GCGCTATGAAACCCTGTTAGC.......................................................................................................................

2.00

................................................................................................................CGCTATGAAACCCTGTTAGCC......................................................................................................................

1.50

....................................CTCGTCACAAACTTCACGCAA..................................................................................................................................................................................................

0.50

................................................................................................................CGCTATGAAACCGTGTTAGCC......................................................................................................................

0.50

.................................................TCATGCACCTCACCGTGCCGA.....................................................................................................................................................................................

0.50

..................................................................................................................CTATGAAACCCTGTTAGCCTC....................................................................................................................

0.50

..................................................CATGCACCTCACCGTGCCGAT....................................................................................................................................................................................

0.50

................................................................................................................CGCTATGAAACCCTGTTAGC.......................................................................................................................

0.50

...............................................................................................................GCGCTATGAAACACTGTCAGA.......................................................................................................................

0.50

.................................................................................................................GCTATGAAACCCTGTTAGC.......................................................................................................................

0.50

.................................................................................................................GCTATGAAACCCTGTTAGCCT.....................................................................................................................

0.50

...............................................................................................................GCGCTATGAAACCCCGTTAGC.......................................................................................................................

0.50

..................................................................................AGATAAGTTGGCCTTTCGCGG....................................................................................................................................................

0.33

......................................................................................AGTTTGGCCTATCGGGGCTT.................................................................................................................................................

0.10

Species	Coordinate	ID	Alignment
droAna3	scaffold_13417:769434-769684 +	dan_3280	CGTAAGCAGTACGCTAAGACCCTCCTGGGCCTGAATGATCAGTGTTTGAAGTACGTGGAGTGGCACGGCTATAATGAAGCATTCTATCCAACCGAAAAGCCCCGAAGTCGTCGCGATACTTTGGGACAATCGGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
droBip1	scf7180000396583:641607-641736 -		CGATCGAATTTCACTAGAAGCCTACAGGGACTGGTTGATCAGTGTAAGGTATACGTGGAGTGGCACGGCTATGATATAGCATTTTATCCTCCCTCAAAGCCCCGAAGCCGTCGGGATACATCG---CAATCGG----------------------------------------------------------------------------------------------------------------------

Species

Coordinate

Alignment

droAna3

scaffold_13417:769434-769684 +

dan_3280

CGTAAGCAGTACGCTAAGACCCTCCTGGGCCTGAATGATCAGTGTTTGAAGTACGTGGAGTGGCACGGCTATAATGAAGCATTCTATCCAACCGAAAAGCCCCGAAGTCGTCGCGATACTTTGGGACAATCGGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN

droBip1

scf7180000396583:641607-641736 -

CGATCGAATTTCACTAGAAGCCTACAGGGACTGGTTGATCAGTGTAAGGTATACGTGGAGTGGCACGGCTATGATATAGCATTTTATCCTCCCTCAAAGCCCCGAAGCCGTCGGGATACATCG---CAATCGG----------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droAna3

CGTAAGCAGTACGCTAAGACCCTCCTGGGCCTGAATGATCAGTGTTTGAAGTACGTGGAGTGGCACGGCTATAATGAAGCATTCTATCCAACCGAAAAGCCCCGAAGTCGTCGCGATACTTTGGGACAATCGGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
...............................................................................................................CGCGATACTTTGGGACAATCG.......................................................................................................................	21	2	11.00	22	13	0	0	1	1	7
..............................................................................................................TCGCGATACTTTGGGACAATC........................................................................................................................	21	1	8.00	8	7	0	0	0	1	0
..........................................................................................ACCGAAAAGCCCCGAAGTCGT............................................................................................................................................	21	1	8.00	8	7	0	0	0	1	0
...........................................................................................................TCGTCGCGATACTTTGGGACA...........................................................................................................................	21	1	5.00	5	4	0	0	1	0	0
.........................................................................ATGAAGCATTCTATCCAACCG.............................................................................................................................................................	21	1	4.00	4	4	0	0	0	0	0
.........................................................................................................AGTCGTCGCGATACTTTGG...............................................................................................................................	19	1	4.00	4	3	0	0	1	0	0
...............................................GAAGTACGTGGAGTGGCACGG.......................................................................................................................................................................................	21	2	4.00	8	3	0	0	0	3	2
...........................................GTTTGAAGTACGTGGAGTGGC...........................................................................................................................................................................................	21	1	3.00	3	1	0	0	1	0	1
...............................................................................................AAAGCCCCGAAGTCGTCGCGA.......................................................................................................................................	21	1	3.00	3	0	0	0	1	1	1
....................................................................................................CCCGAAGTCGTCGCGATACTT..................................................................................................................................	21	1	3.00	3	3	0	0	0	0	0
............................................TTTGAAGTACGTGGAGTGG............................................................................................................................................................................................	19	1	3.00	3	1	0	0	0	2	0
.........................................................................................................AGTCGTCGCGATACTTTGGGA.............................................................................................................................	21	1	3.00	3	1	0	0	0	1	1
....................................................................................TATCCAACCGAAAAGCCCCGA..................................................................................................................................................	21	1	3.00	3	2	0	0	0	1	0
............................................................................................................CGTCGCGATACTTTGGGACA...........................................................................................................................	20	1	3.00	3	3	0	0	0	0	0
........................................AGTGTTTGAAGTACGTGGAGT..............................................................................................................................................................................................	21	1	2.00	2	1	0	0	0	0	1
................................GAATGATCAGTGTTTGAAGTA......................................................................................................................................................................................................	21	1	2.00	2	0	0	0	0	0	2
..............................................................................................AAAAGCCCCGAAGTCGTCGCG........................................................................................................................................	21	1	2.00	2	2	0	0	0	0	0
.....................................................................TATAATGAAGCATTCTATCCA.................................................................................................................................................................	21	1	2.00	2	1	0	0	0	1	0
............................................TTTGAAGTACGTGGAGTGGC...........................................................................................................................................................................................	20	1	2.00	2	2	0	0	0	0	0
..............................................................................................................TCGCGATACTTTGGGACAAT.........................................................................................................................	20	1	2.00	2	1	0	0	0	0	1
.......................................................................................................GAAGTCGTCGCGATACTTTGG...............................................................................................................................	21	1	2.00	2	2	0	0	0	0	0
........................CTGGGCCTGAATGATCAGTGT..............................................................................................................................................................................................................	21	1	2.00	2	0	0	0	0	0	2
.................................................................................................AGCCCCGAAGTCGTCGCGATA.....................................................................................................................................	21	1	2.00	2	0	0	0	0	0	2
..................................................GTACGTGGAGTGGCACGGCTA....................................................................................................................................................................................	21	2	1.50	3	1	0	0	0	0	2
..................................................................................................................GATACTTTGGGACAATCGGAG....................................................................................................................	21	2	1.50	3	2	0	0	0	1	0
...........................................................................GAAGCATTCTATCCAACCGAA...........................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
..........................................TGTTTGAAGTACGTGGAGTGG............................................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
...........CGCTAAGACCCTCCTGGGCCTGAAT.......................................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	1
..........................................................................TGAAGCATTCTATCCAACCGA............................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
............................GCCTGAATGATCAGTGTTTGA..........................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	0	1
.....................................................CGTGGAGTGGCACGGCTATAA.................................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
.......................................................................TAATGAAGCATTCTATCCAAC...............................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
..........................................................................................................GTCGTCGCGATACTTTGGGAC............................................................................................................................	21	1	1.00	1	0	0	0	0	1	0
.........................................................................................AACCGAAAAGCCCCGAAGTCG.............................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
...........................................................................................................TCGTCGCGATACTTTGGGAC............................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
...................................TGATCAGTGTTTGAAGTACGT...................................................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
.................................................................................................................CGATACTTTGGGACAATCGGA.....................................................................................................................	21	2	1.00	2	2	0	0	0	0	0
.........................................GTGTTTGAAGTACGTGGAGTGG............................................................................................................................................................................................	22	1	1.00	1	1	0	0	0	0	0
..........................................................................................ACCGAAAAGCCCCGAAGTCG.............................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
.........................................................................................................AGTCGTCGCGATACTTTGGG..............................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
..........................................................................TGAAGCATTCTATCCAACCG.............................................................................................................................................................	20	1	1.00	1	0	0	0	0	1	0
...............................................................................................................CGCGATACTTTGGGACAATC........................................................................................................................	20	2	1.00	2	2	0	0	0	0	0
.................................AATGATCAGTGTTTGAAGTAC.....................................................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
................................GAATGATCAGTGTTTGAAGTACGTG..................................................................................................................................................................................................	25	1	1.00	1	0	0	0	0	0	1
...........................................................................................CCGAAAAGCCCCGAAGTCGTC...........................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
..................................................................................TCTATCCAACCGAAAAGCCCC....................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
.................................AATGATCAGTGTTTGAAGT.......................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	1	0
........................................................................AATGAAGCATTCTATCCAACC..............................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
..........................................................................................................GTCGTCGCGATACTTTGGGA.............................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
...........................................GTTTGAAGTACGTGGAGTGG............................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
.........................................................................ATGAAGCATTCTATCCAACC..............................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
................................................................................................AAGCCCCGAAGTCGTCGCGAT......................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
.................................................................................................AGCCCCGAAGTCGTCGCGATAC....................................................................................................................................	22	1	1.00	1	1	0	0	0	0	0
......................................TCAGTGTTTGAAGTACGTGGAG...............................................................................................................................................................................................	22	1	1.00	1	1	0	0	0	0	0
...................................................................................CTATCCAACCGAAAAGCCCCG...................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
.................................................................................................AGCCCCGAAGTCGTCGCGAT......................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
...........................................................................................CCGAAAAGCCCCGAAGTCG.............................................................................................................................................	19	1	1.00	1	1	0	0	0	0	0
.............................................TTGAAGTACGTGGAGTGGCAC.........................................................................................................................................................................................	21	2	1.00	2	2	0	0	0	0	0
...........................................................................................CCGAAAAGCCCCGAAGTCGTCG..........................................................................................................................................	22	1	1.00	1	1	0	0	0	0	0
..................................................................................................GCCCCGAAGTCGTCGCGATA.....................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
......................................................................................TCCAACCGAAAAGCCCCGAAGT...............................................................................................................................................	22	1	1.00	1	1	0	0	0	0	0
..............................................................................................................TCGCGATACTTTGGGACAATCG.......................................................................................................................	22	1	1.00	1	0	0	0	0	1	0
...............................................................CACGGCTATAATGAAGCATTC.......................................................................................................................................................................	21	1	1.00	1	0	0	0	1	0	0
...........................................................................GAAGCATTCTATCCAACCGA............................................................................................................................................................	20	1	1.00	1	0	0	0	0	0	1
................................................................................................AAGCCCCGAAGTCGTCGC.........................................................................................................................................	18	1	1.00	1	1	0	0	0	0	0
.................................................AGTACGTGGAGTGGCACGG.......................................................................................................................................................................................	19	2	1.00	2	2	0	0	0	0	0
...........................................................................................CCGAAAAGCCCCGAAGTCGT............................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
..................................ATGATCAGTGTTTGAAGTACG....................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0
...................................................TACGTGGAGTGGCACGGC......................................................................................................................................................................................	18	2	0.50	1	1	0	0	0	0	0
...............................................GAAGTACGTGGAGTGGCACGGC......................................................................................................................................................................................	22	2	0.50	1	0	0	0	0	0	1
................................................................................................................GCGATACTTTGGGACAATCGG......................................................................................................................	21	2	0.50	1	0	0	0	0	1	0
.................................................AGTACGTGGAGTGGCACGGC......................................................................................................................................................................................	20	2	0.50	1	1	0	0	0	0	0
.............................................TTGAAGTACGTGGAGTGGCACGG.......................................................................................................................................................................................	23	2	0.50	1	1	0	0	0	0	0
...............................................GAAGTACGTGGAGTGGCACG........................................................................................................................................................................................	20	2	0.50	1	1	0	0	0	0	0
...............................................................................................................CGCGATACTTTGGGACAATCGC......................................................................................................................	22	2	0.50	1	1	0	0	0	0	0
..............................................TGAAGTACGTGGAGTGGCTT.........................................................................................................................................................................................	20	2	0.50	1	0	0	0	0	0	1

droBip1

CGATCGAATTTCACTAGAAGCCTACAGGGACTGGTTGATCAGTGTAAGGTATACGTGGAGTGGCACGGCTATGATATAGCATTTTATCCTCCCTCAAAGCCCCGAAGCCGTCGGGATACATCG---CAATCGG----------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

dan_3280

scaffold_13417:769484-769634 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-9.6	-9.6	-9.5