ID:

dan_2722

 Coordinate:

scaffold_13337:19347974-19348124 +

 Confidence:

Candidate

 Type:

Unknown

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-9.4 -9.0 -8.8 -8.8

Flybase annnotation

exon [dana_GLEANR_10796:3]; CDS [Dana\GF10838-cds]; intron [Dana\GF10838-in]

No Repeatable elements found

Sense Strand Reads

##################################################---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
GAACCCGAAATAGAGCATGAGGAGCATGAAGAACCGATCCCAAAACCAAAGTATTATAAACGCAAGAAGTCTTTAATTAGCCTTGAGAGAGAAAGGTTGGCTGTCCAGAAGGAAACCTTAAATGTAATGCGATCCATGGTTAGGGAAATGTCAAACTTTCATGCCTCGTTTTTAATTACATACAAAAAACAAAAAAAACGCTCCACATAATTAAGTTAAAACCTCGAATCCTACATTCAATACCTGATTGA
***********************************************************************((......((...........((((((.....(((....))))))))).........)).......))**************************************************************************************************************** 		Read size # Mismatch Hit Count Total Norm Total M058
embryo
 V039
embryo
 M044
female body
 V055
head
 V105
male body
.......................................................................TTTAATTAGCCTTGAGAGAGAAAG............................................................................................................................................................ 24 0 1 1.00 1 1 0 0 0 0
......................................................................................................................TAAATGTAATGCGATCCATGG................................................................................................................ 21 0 1 1.00 1 0 1 0 0 0
..............................................................................................................................ATGCGATCCATGGTTAGGG.......................................................................................................... 19 0 1 1.00 1 0 0 1 0 0
..............................................................................................................................................................................................AAAAAAAACGCTCCGCAGAA......................................... 20 2 2 0.50 1 0 1 0 0 0
..................GAGGAGCCTGAATATCCGATC.................................................................................................................................................................................................................... 21 3 2 0.50 1 0 0 0 1 0
......................TGGATGAAGAACGGATCCCAA................................................................................................................................................................................................................ 21 3 3 0.33 1 0 0 0 1 0
........................................................................................................................................................................................AAAGACAGAAAAAACGCACCAC............................................. 22 3 5 0.20 1 1 0 0 0 0
...............................................................................................................................................................................................AAAAAAACGCTCCACACCA......................................... 19 2 6 0.17 1 1 0 0 0 0
.........................................................................................................................................................................................AAAACAAAAAAAACTCCCCACA............................................ 22 2 6 0.17 1 1 0 0 0 0
........................................................................................................................................................................................AAAAACAAAAACAACCCTCCCCA............................................ 23 3 8 0.13 1 1 0 0 0 0
.........................................................................................................CAGAAGGATAACTTAAATGC.............................................................................................................................. 20 3 13 0.08 1 0 0 0 0 1
........................................................................................................GCAGAAGGATAACTTAAATG............................................................................................................................... 20 3 20 0.05 1 0 0 0 0 1
..........................................................................................................................................................................................AAACACAAAAAACGCACCAGA............................................ 21 3 20 0.05 1 0 1 0 0 0
....................................................................................................................................................................................................................AAGTTAGAGGCTCGAATCC.................... 19 3 20 0.05 1 0 1 0 0 0
.................................................................................................................................................AAATGTCGAACTTTCTTG........................................................................................ 18 2 20 0.05 1 0 1 0 0 0
..................GAGGAGCATGGATATCCGA...................................................................................................................................................................................................................... 19 3 20 0.05 1 0 0 0 1 0
..........................................................................................................................................................................................AACCAAAAAAAACGCACCA.............................................. 19 2 20 0.05 1 1 0 0 0 0
..........................................................................................................................................................................................AAACAAAAAAAACGCTAGA.............................................. 19 2 20 0.05 1 1 0 0 0 0

Anti-sense strand reads

CTTGGGCTTTATCTCGTACTCCTCGTACTTCTTGGCTAGGGTTTTGGTTTCATAATATTTGCGTTCTTCAGAAATTAATCGGAACTCTCTCTTTCCAACCGACAGGTCTTCCTTTGGAATTTACATTACGCTAGGTACCAATCCCTTTACAGTTTGAAAGTACGGAGCAAAAATTAATGTATGTTTTTTGTTTTTTTTGCGAGGTGTATTAATTCAATTTTGGAGCTTAGGATGTAAGTTATGGACTAACT
****************************************************************************************************************((......((...........((((((.....(((....))))))))).........)).......))*********************************************************************** 		Read size # Mismatch Hit Count Total Norm Total M044
female body
 M058
embryo
 V039
embryo
 V055
head
....................................................................................................................................................ACAGTTTGGAAGTACGGAGC................................................................................... 20 1 1 1.00 1 1 0 0 0
........TTATCTAGTACTCCTCGTACTTCTTGGC....................................................................................................................................................................................................................... 28 1 1 1.00 1 1 0 0 0
...................................................................................................CGACAGGTCTTCCTTTGGAAT................................................................................................................................... 21 0 1 1.00 1 1 0 0 0
........................................................................................................................TTACATTACGCTAGGTACCAAT............................................................................................................. 22 0 1 1.00 1 0 1 0 0
......CTTTATCTCGTACTCCTCGTA................................................................................................................................................................................................................................ 21 0 1 1.00 1 1 0 0 0
....................................................................................................................................................................................................................................AGGATGTGAGTTATGGAATAT.. 21 3 6 0.17 1 0 0 1 0
.......................................................................................................................................................GTTTGAGAGTAGGGAGC................................................................................... 17 2 20 0.05 1 0 0 0 1

Re-alignment of all predicted orthologs

Species Coordinate ID Alignment
droAna3 scaffold_13337:19347924-19348174 + dan_2722 GAACCCGAAATAGAGCATGAGGAGCATGAAGA------ACCGATCCCAAAACCAAAGTATTATAAACGCAAGAAGTCTTTAATTAGCCT-TGAGAGAGAAAGGTTGGCTGTCCAGAAGGAAACCTTAAATGTAATGCGATCCATGGTTAGGGAAATGTCAAACTTTCATGCCTCGTTTTTAATTACATACAAAA---AACAAAAAAAACGCTCCACATAATTAAGTTAAAACCTCGAATCCTACATTCAATACCTGAT------------------------------------------------------------------------------TGA
droBip1 scf7180000396360:541066-541312 + GAACCCGAAATTGAATCTGAGA---TTGAAGAATTACAACCGGTCTCCAACGAAAAGTATTTTAAACGCAAGCGGTCGATTTTAAGAAT-TGAGCAAGAAAAAATAGCTGTCCAGAAGCAGACCTTAAATATAATGCGGTCGATGGTTAAGGAAATTTCAAACTTTCATGCGTCATTTTTAATTACTTACAAGACACAAAACAAAAAACGCTCTACGTAGTTAAATTCAGACC----------CATTAATTAGCTGAT------------------------------------------------------------------------------GGA
droKik1 scf7180000302686:355171-355337 - G-------------------------------------------------------------------------------------------AGGAAAAAAAACTGGCTATTCAAAAAGAAATACTCCACGTAATGCAAACAATGGCAAACGATCTTTCCAGTACATATACCACCTTTCTAGATGCTTACAAAACAAGTCCGACGAAACGCTTTTGATAGTTCAAATTAGATGTAGATAAATACAA---AAAACCAAA------------------------------------------------------------------------------TAT
droFic1 scf7180000454113:2398535-2398678 + A------------------------------------------------------------------------------------GCTGTGGAAGAAAAAACTTTGGCCGTTCAACGAAAAACCCTTAAAGCCATGCAAGCAATGGCCGAAGATCTTTCCAGTTTTCATGAAAATTTCCTTAAGGCGATCAAACCAACATTCCAAAAACACGCTTAATAGCC---------------------------------AAT------------------------------------------------------------------------------TAA
droEle1 scf7180000491193:4193002-4193212 - A------------------------------------------------------AGCGCTATAAGCGCAAACGGACCA----AAGCTGTGGGAGAAGAAATGTTGGCCTTGCAAAGAGAAACACTCGACGTCCTGCGAAATATTGCAAAAGACCTTTCCAGTTTTCATGAAAAGTTCTTAAATGCACTCAAGCCCATGCATCAGCGACGCTCTTCATAGTTATATTAAGT---------------------------TATTTAAGGAATACTATTAGTAGTTATGA--------C---------------------------TTTC--------TTTT
droRho1 scf7180000773422:10816-11071 + C---------------------------------------TGAACTCAA-CGCTAAACGCTATAAGCACAAACGGGCCA----AAGCAATGGGAGAAGAAATGTTGGCTCTTCAAAGAGAAACTCTCAACGTCATGCGAACGATGGCAAAGGACCTTTCCAGCTTTCATGACAAGTTCTTGAAAGCACTAAAACCAACTCACCACAAACGCTCTTCATAGCTAGAATAAAT---------------------------TACTTAAAAAATTATATTTGTTATTATGATATGACAACATTCTTTCAA----AAGGTGCAAAATATTTTTG--------CT
droBia1 scf7180000302428:1388803-1389008 + ------------------------------------------------------------------------------------------------AGAAATGTTGGCCCTTCAAAGGGAAACTCTCAAGGTCTTGCAGACAATTTCAGAGAACCTCTCCAGTTTCCACGATAAGTTCTTGAGAGCACTCAAGCCAAGACGCGAGAAGCGCCCTA--------GACTAAGT---------------------------TAGCTAAGAGCT-GCATTTGTTAT-ATATTAAGAGAATTTTCCTACATACGTAATATGCAAAATATTTTTGCAAAACTTGT
droTak1 scf7180000415420:174209-174345 + T-----------------------------------------------------------------------------------------TGGCGAAGAAATGCTGGCCCTTCAAAGGGAAACTCTAAATGTTATGCGAACTATTGCAGAGGATCTTTCCAGTTTTCATGACAAGTTCTTGAAAGCATTCGCGCCAAAACGCCAGAAACGCTCTA--------GACTAAGC---------------------------TAA------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droAna3
GAACCCGAAATAGAGCATGAGGAGCATGAAGA------ACCGATCCCAAAACCAAAGTATTATAAACGCAAGAAGTCTTTAATTAGCCT-TGAGAGAGAAAGGTTGGCTGTCCAGAAGGAAACCTTAAATGTAATGCGATCCATGGTTAGGGAAATGTCAAACTTTCATGCCTCGTTTTTAATTACATACAAAA---AACAAAAAAAACGCTCCACATAATTAAGTTAAAACCTCGAATCCTACATTCAATACCTGAT------------------------------------------------------------------------------TGASizeHit CountTotal NormTotalM044
Female-body		M058
Embryo		V039
Embryo		V055
Head		V105
Male-body		V106
Head
.............................................................................TTTAATTAGCCT-TGAGAGAGAAAG.............................................................................................................................................................................................................................................2411.001010000
.............................................................................................................................TAAATGTAATGCGATCCATGG.................................................................................................................................................................................................2111.001001000
.....................................................................................................................................ATGCGATCCATGGTTAGGG...........................................................................................................................................................................................1911.001100000
droBip1
GAACCCGAAATTGAATCTGAGA---TTGAAGAATTACAACCGGTCTCCAACGAAAAGTATTTTAAACGCAAGCGGTCGATTTTAAGAAT-TGAGCAAGAAAAAATAGCTGTCCAGAAGCAGACCTTAAATATAATGCGGTCGATGGTTAAGGAAATTTCAAACTTTCATGCGTCATTTTTAATTACTTACAAGACACAAAACAAAAAACGCTCTACGTAGTTAAATTCAGACC----------CATTAATTAGCTGAT------------------------------------------------------------------------------GGASizeHit CountTotal NormTotal
No Reads
droKik1
G-------------------------------------------------------------------------------------------AGGAAAAAAAACTGGCTATTCAAAAAGAAATACTCCACGTAATGCAAACAATGGCAAACGATCTTTCCAGTACATATACCACCTTTCTAGATGCTTACAAAACAAGTCCGACGAAACGCTTTTGATAGTTCAAATTAGATGTAGATAAATACAA---AAAACCAAA------------------------------------------------------------------------------TATSizeHit CountTotal NormTotal
No Reads
droFic1
A------------------------------------------------------------------------------------GCTGTGGAAGAAAAAACTTTGGCCGTTCAACGAAAAACCCTTAAAGCCATGCAAGCAATGGCCGAAGATCTTTCCAGTTTTCATGAAAATTTCCTTAAGGCGATCAAACCAACATTCCAAAAACACGCTTAATAGCC---------------------------------AAT------------------------------------------------------------------------------TAASizeHit CountTotal NormTotal
No Reads
droEle1
A------------------------------------------------------AGCGCTATAAGCGCAAACGGACCA----AAGCTGTGGGAGAAGAAATGTTGGCCTTGCAAAGAGAAACACTCGACGTCCTGCGAAATATTGCAAAAGACCTTTCCAGTTTTCATGAAAAGTTCTTAAATGCACTCAAGCCCATGCATCAGCGACGCTCTTCATAGTTATATTAAGT---------------------------TATTTAAGGAATACTATTAGTAGTTATGA--------C---------------------------TTTC--------TTTTSizeHit CountTotal NormTotal
No Reads
droRho1
C---------------------------------------TGAACTCAA-CGCTAAACGCTATAAGCACAAACGGGCCA----AAGCAATGGGAGAAGAAATGTTGGCTCTTCAAAGAGAAACTCTCAACGTCATGCGAACGATGGCAAAGGACCTTTCCAGCTTTCATGACAAGTTCTTGAAAGCACTAAAACCAACTCACCACAAACGCTCTTCATAGCTAGAATAAAT---------------------------TACTTAAAAAATTATATTTGTTATTATGATATGACAACATTCTTTCAA----AAGGTGCAAAATATTTTTG--------CTSizeHit CountTotal NormTotal
No Reads
droBia1
------------------------------------------------------------------------------------------------AGAAATGTTGGCCCTTCAAAGGGAAACTCTCAAGGTCTTGCAGACAATTTCAGAGAACCTCTCCAGTTTCCACGATAAGTTCTTGAGAGCACTCAAGCCAAGACGCGAGAAGCGCCCTA--------GACTAAGT---------------------------TAGCTAAGAGCT-GCATTTGTTAT-ATATTAAGAGAATTTTCCTACATACGTAATATGCAAAATATTTTTGCAAAACTTGTSizeHit CountTotal NormTotal
No Reads
droTak1
T-----------------------------------------------------------------------------------------TGGCGAAGAAATGCTGGCCCTTCAAAGGGAAACTCTAAATGTTATGCGAACTATTGCAGAGGATCTTTCCAGTTTTCATGACAAGTTCTTGAAAGCATTCGCGCCAAAACGCCAGAAACGCTCTA--------GACTAAGC---------------------------TAA------------------------------------------------------------------------------SizeHit CountTotal NormTotal
No Reads

Generated: 05/18/2015 at 05:13 AM