dan_2654

##################################################--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- AGTTCAGTCCGGAGCCACCGACCCATATCTTCTGGTATCATCAGGACAAGGTAAATATCGGCGAACTCTCTTAAAATCAATGTACAGACTAGCGAGCAAAAATATAAAAAAAAAAAACAACAGAGCTCAAAGGGCGAGGGGAAAATCCAATTTAAGGTTTTCCTTTTTGGTGCCAAATTATCTTGCAATAATTGAGTTTGTGCGCAGTAAAAAGTCGCGAAAAAAGCAGAGAAGGGGCGGGGCCAGGACCC ************************************************..........((((((((..(((.......((((.(((.....((((...........................))))....(((((((((((((((........))))))))))))....)))......))))))).)))..))))))))************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M058 embryo	V105 male body	V039 embryo	V106 head
.GTTCAGTCCGGAGCCACCGACCC...................................................................................................................................................................................................................................	23	0	1	1.00	1	1	0	0	0
..........................................................................................................................................................................................................................GAAAAAAGAAGAGAAGGG...............	18	1	1	1.00	1	1	0	0	0
............................................................................................................AAAAAAAAACTACAGAGGTCGAA........................................................................................................................	23	3	2	0.50	1	0	1	0	0
........CCAGCGCCCCCGACCCATATC..............................................................................................................................................................................................................................	21	3	4	0.25	1	0	0	1	0
................................................................................................................................AAATGGCGCGGGGTAAATC........................................................................................................	19	3	20	0.10	2	0	0	0	2
..............................................CAAGGTAACTTTCGTCGAA..........................................................................................................................................................................................	19	3	20	0.05	1	0	0	1	0

TCAAGTCAGGCCTCGGTGGCTGGGTATAGAAGACCATAGTAGTCCTGTTCCATTTATAGCCGCTTGAGAGAATTTTAGTTACATGTCTGATCGCTCGTTTTTATATTTTTTTTTTTTGTTGTCTCGAGTTTCCCGCTCCCCTTTTAGGTTAAATTCCAAAAGGAAAAACCACGGTTTAATAGAACGTTATTAACTCAAACACGCGTCATTTTTCAGCGCTTTTTTCGTCTCTTCCCCGCCCCGGTCCTGGG ************************************************..........((((((((..(((.......((((.(((.....((((...........................))))....(((((((((((((((........))))))))))))....)))......))))))).)))..))))))))************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M058 embryo	M044 female body	V055 head	V106 head
..........CCTCGGTGGCTGGTTATACAAA...........................................................................................................................................................................................................................	22	3	1	2.00	2	1	1	0	0
......................................................................................CTGATCGCAAGTTTTTATAT.................................................................................................................................................	20	2	2	0.50	1	0	0	1	0
..................................................................................................................................................................................................CCAAACACGCGTCATTTAT......................................	19	2	3	0.33	1	1	0	0	0
............TCGGTGGCTGGTTATACAAA...........................................................................................................................................................................................................................	20	3	12	0.25	3	0	3	0	0
..........................TAGAAGACGATAGGACTCC..............................................................................................................................................................................................................	19	3	17	0.06	1	0	0	0	1

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droAna3	scaffold_13337:15054729-15054979 -	dan_2654	AGTTCAGTCCGGAGCCACCGACCCATATCTTCTGGTATCATCAGGACAAGGTAAATATCGGCGA---------------------------------------------------ACTCTCT------TAAAATCAATGTACAGA----------CTA-------------------------------------------GCGAGCAAAAATA------TAA------AAAAA--------AAAAACAACAGAG------------------CTCAAAGGG-CGAGG-G-----------GAAAATCCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCTTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAGT--CGC----------GAAAAAA---GCAGAG-------------------------------------------------------------------------------------------------------------------------------------------AAG-GG------------GCGGGGCCAGG--ACCC
droBip1	scf7180000395155:983549-983781 -		AGTTCAGTCCGGAGCCACCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATATCGGCGA---------------------------------------------------GCTCTCT------TAAAATCAATTTACAGA----------CTA-------------------------------------------GCGAGCAAAAATA------TAA------AAAAAG------------------------------------AACTTAAAGGGC-GAGGGG-----------GAAAAACCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCTTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAGTCGCAC------------AAAAAAAAGTAGAG-----------------------------------------------------------------------------------------------------------------------------------------------GG------------GCGGGG-----------
droKik1	scf7180000302334:304292-304538 +		AGTTCAGCCCGGAGCCGCCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATAT------------------------------------------------------------CCCT------TAAAATCAATTTACAGTGCACGGGAGACGAAAAA-------------------------------TA--AC------------------T--------------------------------------------CC-------------TGCGGGCGAGG-G-----------GAAAAACCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCTTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAA---GCCGGG-GAGACG------------------------------------------------------------------------AAGTAGCA-GG-A-GGAGGAGGAGCAAT---------------------------------AG-GG------------GC-GGGCCAC-------
droFic1	scf7180000453841:923318-923561 +		AGTTCAGCCCCGAGCCGCCGACCCATATCTTCTGGTATCATCAGGACAAGGTAAATAT------------------------------------------------------------CTCT------TGAAATCAATTTACAGC-----------AAAAAG---------AAAAATG---------------TA--ACC-------------------------AA--AA-----------------------C-------C-------------------CTGTGG-GGCAGAAG-GAAGTAAAACCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCTTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAG----TGGGGCGTGTTG-------------------------------------------------------------------------TGGAGTG-AG-G-GGC---------AG--------------------------------AGG-GG------------GCGGGGCCAC-------
droEle1	scf7180000490564:956424-956611 -		AGTTCAGCCCGGAGCCGCCGACCCATATCTTCTGGTATCATCAGGACAAGGTAAATAT------------------------------------------------------------CTGT------TGAAATCAATTTAGAGG----------G-----GCGACAG-------------------------TGAG-CC-------------------------A----------------------------------------------------------------------------AAAACCCAATTTAAGGTTTTTCCTTTTTGG-TGCCAAATTATCCTGCAATAATTGAGTTTG-TG--------------------------------AGCAGTAAAAAG---CGC------------AAGAG--------------------------------------------------------------------------------------------------------------------------------------------------------GG------------GCGGTGCCAC-------
droRho1	scf7180000780105:266482-266730 +		AGTTCAGCCCGGAGCCGCCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATATA----------------------------------------------------------TCTCT------TAAAATCAATTTACAGG----------GGAAAAG-------------------------------TAAG-CC-------------------------AA--AA---------------------------------------------------CCGAGG-GGTGTTAGGGGGGAAAACCCAATTTAAGGTT-TTCCTTTTCGG-TGCCAAATTATCTTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAA---CGC------------CAAAG----TGTG-----TGG--------------------------------------------------------------ACGAG------GGGGGAG-GGGG-GAGG--------------------GGAAG-----------GTT-GA-GG-AG------------GCGGTGCCAC-------
droBia1	scf7180000302428:7619799-7620045 +		AGTTCAGCCCGGAGCCGCCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATAT------------------------------------------------------------CTCT------TAAAATCAATTTGCATG----------G----------------------------------------------------AAAATA------TAAGTAA--AA--AGAAGC---------GAAAAAGC-------C------------AGGATG-CGAGGGG-----------TAAAAACCAATTTAAGGTT-TTCCTTTTTGG-CGCCAAATTATCTTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAG----TGGGGCAGGTTG---------------------------------------------------------------------TGGGG-------------GAG--------------------GTG-G-----------GCC---AAA-GG------------GCGGTGCCAC-------
droTak1	scf7180000415209:157287-157526 -		AGTTCAGCCCGGAGCCGCCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATAT------------------------------------------------------------CTCT------TAAAATCAATTTACAGC----------A----------------------------------------------------AAAAAAAGTTACT----AA--AAAAAG--A---A--------AAGAGC-----------------------GGGC-CAGGGG-----------GAAAAACCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCTTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAG----TGGAGCATGGGT------------------------------------------------------------------------GGC-------------AG--------------------GGG-G-----------CGT---AAG-AG------------GCGGGGCCAC-------
droEug1	scf7180000409711:1997659-1997925 -		AGTTTAGCCCAGAGCCGCCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATAT------------------------------------------------------------CTCT------TAAAATCAATTTGCAGG----------CTAAAAG-------------------------------TA--ACC-------------------------AA--ATAA--AAAC---------GAAAAAGCGGCAAACC-------------TGCGGGCGAGG-GGGAA-----------GACCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCTTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAG----TGGAGCGCGTTT----------------------------------------------------------------------CGGTGAGGTG-GG-CAGATG--------------------GTGGG-----------GTTCAAAAG-AG------------GCGGGGCCAC-------
dm3	chr3L:10143874-10144125 -		AGTTCAGCCCGGAGCCACCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATAT------------------------------------------------------------CTCT------TAAAATCAATTTAC--------------TAAAAG-------------------------------TA--ACC-------------------------AA--AAAA--AAAAAAAAAAA--------G---CAAACAA------AAATTAAGCGGCCGCGG-GGCCA-----------TAGCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCGTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAG----TGGGG-----------------------------------------------------------------------------------GGCG-CG-A-GGAG--------------------GTAAG-----------GAT---AAA-GG------------GCGGGGCCATG------
droSim2	3l:9888340-9888600 -		AGTTCAGCCCGGAGCCACCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATAT------------------------------------------------------------CTCT------TAAAATCAATTTACAGG----------CTAAAAG-------------------------------TA--ACC-------------------------AA--CAAA--AA-----AAAA--------G---CAAACAA------AAATTAAGCGGCCGCGG-GGCAA-----------TAGCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCGTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAG----TGGAGCGTGTTG------------------------------------------------------------------------GTGGGGCG-CG-A-GGAG--------------------GTAAG-----------GAT---AAA-GG------------GCGGGGCCAC-------
droSec2	scaffold_0:2359455-2359717 -		AGTTCAGCCCGGAGCCACCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATAT------------------------------------------------------------CTCT------TAAAATCAATTTACAGG----------CTAAAAG-------------------------------TA--ACC-------------------------AA--CAAA--AAA---AAAAA--------G---CAAACAA------AAATTAAGCGGCCGCGG-GGCAA-----------TAGCAATTTAAGATT-TTCCTTTTTGG-TGCCAAATTATCGTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAG----TGGAGCGTGTGG-------------------------------------------------------------------------GGGGGTGGAG-A-GGAG--------------------GTAAG-----------GAT---AAA-GG------------GAGGGGCCAC-------
droYak3	3L:10126160-10126445 -		AGTTCAGCCCGGAGCCACCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATAT------------------------------------------------------------CTCT------TAAAATCAATTTACAGG----------CTAAAAG-------------------------------TA--ACCAGC--GCAAAAATA------CAA------AAAAA--AA---AAAGAACAAAAATGC-------CAAAAAACAAATTAAGCGGCCGAGG-GGCAA-----------TAGCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCGTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAG----TGGAGCGTGTGC------------------------------------------------------------------------GGGGGGTG-CG-G-GGAG--------------------GTAAG-----------GAT---AAA-GG------------GCGGTGCCAC-------
droEre2	scaffold_4784:10129681-10129944 -		AGTTCAGCCCGGAGCCACCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATAT------------------------------------------------------------CTCT------CGAAATCAATTTACAGG----------CTAAGAG-------------------------------TA--ACC-------------------------AA--AAAA--AAGCAAAAAAA--------GCGAG----AA------AAATTAAGCGGCCGAGG--GCAA-----------TAGCAATTTAAGGTT-TTCCTCTTTGG-TGCCAAATTATCGTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAG----TGGAGCGTGTGC------------------------------------------------------------------------GGGGGGTG-CG-G-GGCG--------------------GTAAG-----------GAT---AAA-GG------------GCGGAGCCAC-------
dp5	XR_group6:8531477-8531772 -		AGTTCAGCCCAGAGCCGCCGACCCATATCTTTTGGTATCACCAGGACAAGGTAAGTAGCGCCGCCGCTCCACTCCGCGCCACTCCACTCTACAC----------CACAGCAAACCACTCCCT------TGAAATCAATTTACAGG----------G--AAAG-------------------------------CG--CCC-------------------------AC--AGAACGAAC---------------------------------------------------------------GAAAAACCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCTTGCAATAATTGAGTTTGGTG--------------------------------CGCCGTAAA-AG---CGC------------AA--G----TACA-------------------------------------------------------------------AGGGCAAG---------------------------GGCAAGAGGGATCGGGATCGG-----------GATCGA-GAAGGACGCCCCGCCCCGCCGTGCCAC-------
droPer2	scaffold_27:971252-971563 -		AGTTCAGCCCAGAGCCGCCGACCCATATCTTTTGGTATCACCAGGACAAGGTAAGTAGCGCCGCTGCTACACTCCATGCCACTCCGCGCTACACCACACCACAGCACAGCAAACCACTCCCT------TGAAATCAATTTACAGG----------G--AAAG-------------------------------CG--CCC-------------------------AC--AGAACGAAC---------------------------------------------------------------GAAAAACCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCTTGCAATAATTGAGTTTGGTG--------------------------------CGCCGTAAA-AG---CGC------------AA--G----TACA-------------------------------------------------------------------AGGGCAAG------GGC---------------AAGGGCAAGAGGGATCGGGATTGG-----------GATCGA-GAAGGACGCCCCGCCCCGCCGTGCCAC-------
droWil2	scf2_1100000004837:552528-552874 +		AATTTAGTCCGGAGCCACCTACCCATATCTTTTGGTATCATCAGGATAAGGTAAATGTATACGA----------------------------AT----------CACAGTTCACAGTTC----------CCAATCAATTTTCATA----------CAGAGAG---------TGAGATGGTGGGGAGGGGGGGTTA------GCGAATGAAATTT------CAAGTACACAAAATGAAA---------------------------------------------------A-----------GAAAACCCAATTTAATGTT-TTCCTCTTTGGAAGCCAAATTATCTTGCAATAATTGAGTTTT-TTTCGGTATTTCTCTCCTCCTCCTCCGTTCTCTTAGCAGTTAAAAG---CAC------------AAAAA----AATA---------------------------------------------------------------------TATACATATATGAAGAGTG-GG-G-GATG--------------------GTACCAATGCAGGATAATC-GC-AG-AG------------TCTGAGCCATCGTCACC
droVir3	scaffold_13049:15923731-15923958 -	dvi_20133	AGTTCAGTCCGGAGCCACCAACACACATTTTCTGGTATCATCAGGATAAGGTGAGTAT------------------------------------------------------------CATACCAGAGTCAAATCAATTTATGA---------------GAGTAATAAAAT--AAATG---------------A-----------------------------------------------A--------ACAAG------------------TTTAAA--------------A-----------GATAAATAAAAAATT-TTGTTGTGTGG--ATCTAATTATCTTGCAATAATTGAGTTTTGCC--------------------------------CTCGCTAAAA-G---AAA-------AGTTAAAAAA---TCAGAGCAGC--CACGCCCACAGT---------------------------------------------------T------------------------------------------------------------------CAA----------------------------A--ACCC
droMoj3	scaffold_6680:20876159-20876386 +		AGTTCAGCCCGGAGCCGCCGACACACATCTTCTGGTATCACCAGGACAAGGTAAGC---------------------------------------------------------------------------AATCAATTTGTGA--------------A----------------------------------TA--TTC-------------------------AA--CATA--AGA---CGAAACCAAAAATGCTTT----AA----------------------------A-----------GATGCACTTAAAATT-C-CCGTGTGCG-CATCAAATTATCGTGGAATAATTGAACTTTGCC--------------------------------CTCTCTGAGAAC----TC------------GG--G----CA-----------------------------------CTCAAACTCAAACTGTTTCTCATACCAATACTTAAGCT------------------------------------------------------------------CAA----------------------------G--GCCC
droGri2	scaffold_15110:12733104-12733379 +		AATTTAGTCCGGAGCCACCAACACACATTTTCTGGTATCATCAGGATAAGGTGAGCAT------------------------------------------------------------CGTATCAGAATTCAATCAATTTATGA---------------AAA-------------------------------TG--ACG-------------------------GG--AAAG--AAA---A--------AAAAG---------------------------TCGCTT-TGT-A-----------TATAAATTTAAATTT-TTC--TTTGCG-CCTCAAATTATCTTGCAATAATTGAGTTTTGCC--------------------------------CTCG-----------CACAAAAGGGCGCTGAAAAA---TCAGAGAATC--CACACCCACGATTCACAGCACATGCACTCAAACTCAGAGTGAAACTCATACTCAT---------------------------------------------------------------------------------------------ACCGTAGCACAAG-------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droAna3

AGTTCAGTCCGGAGCCACCGACCCATATCTTCTGGTATCATCAGGACAAGGTAAATATCGGCGA---------------------------------------------------ACTCTCT------TAAAATCAATGTACAGA----------CTA-------------------------------------------GCGAGCAAAAATA------TAA------AAAAA--------AAAAACAACAGAG------------------CTCAAAGGG-CGAGG-G-----------GAAAATCCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCTTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAGT--CGC----------GAAAAAA---GCAGAG-------------------------------------------------------------------------------------------------------------------------------------------AAG-GG------------GCGGGGCCAGG--ACCC	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
.GTTCAGTCCGGAGCCACCGACCC................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................	23	1	1.00	1	0	1	0	0	0	0

droBip1

AGTTCAGTCCGGAGCCACCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATATCGGCGA---------------------------------------------------GCTCTCT------TAAAATCAATTTACAGA----------CTA-------------------------------------------GCGAGCAAAAATA------TAA------AAAAAG------------------------------------AACTTAAAGGGC-GAGGGG-----------GAAAAACCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCTTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAGTCGCAC------------AAAAAAAAGTAGAG-----------------------------------------------------------------------------------------------------------------------------------------------GG------------GCGGGG-----------	Size	Hit Count	Total Norm	Total
No Reads

droKik1

AGTTCAGCCCGGAGCCGCCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATAT------------------------------------------------------------CCCT------TAAAATCAATTTACAGTGCACGGGAGACGAAAAA-------------------------------TA--AC------------------T--------------------------------------------CC-------------TGCGGGCGAGG-G-----------GAAAAACCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCTTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAA---GCCGGG-GAGACG------------------------------------------------------------------------AAGTAGCA-GG-A-GGAGGAGGAGCAAT---------------------------------AG-GG------------GC-GGGCCAC-------	Size	Hit Count	Total Norm	Total
No Reads

droFic1

AGTTCAGCCCCGAGCCGCCGACCCATATCTTCTGGTATCATCAGGACAAGGTAAATAT------------------------------------------------------------CTCT------TGAAATCAATTTACAGC-----------AAAAAG---------AAAAATG---------------TA--ACC-------------------------AA--AA-----------------------C-------C-------------------CTGTGG-GGCAGAAG-GAAGTAAAACCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCTTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAG----TGGGGCGTGTTG-------------------------------------------------------------------------TGGAGTG-AG-G-GGC---------AG--------------------------------AGG-GG------------GCGGGGCCAC-------	Size	Hit Count	Total Norm	Total
No Reads

droEle1

AGTTCAGCCCGGAGCCGCCGACCCATATCTTCTGGTATCATCAGGACAAGGTAAATAT------------------------------------------------------------CTGT------TGAAATCAATTTAGAGG----------G-----GCGACAG-------------------------TGAG-CC-------------------------A----------------------------------------------------------------------------AAAACCCAATTTAAGGTTTTTCCTTTTTGG-TGCCAAATTATCCTGCAATAATTGAGTTTG-TG--------------------------------AGCAGTAAAAAG---CGC------------AAGAG--------------------------------------------------------------------------------------------------------------------------------------------------------GG------------GCGGTGCCAC-------	Size	Hit Count	Total Norm	Total
No Reads

droRho1

AGTTCAGCCCGGAGCCGCCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATATA----------------------------------------------------------TCTCT------TAAAATCAATTTACAGG----------GGAAAAG-------------------------------TAAG-CC-------------------------AA--AA---------------------------------------------------CCGAGG-GGTGTTAGGGGGGAAAACCCAATTTAAGGTT-TTCCTTTTCGG-TGCCAAATTATCTTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAA---CGC------------CAAAG----TGTG-----TGG--------------------------------------------------------------ACGAG------GGGGGAG-GGGG-GAGG--------------------GGAAG-----------GTT-GA-GG-AG------------GCGGTGCCAC-------	Size	Hit Count	Total Norm	Total
No Reads

droBia1

AGTTCAGCCCGGAGCCGCCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATAT------------------------------------------------------------CTCT------TAAAATCAATTTGCATG----------G----------------------------------------------------AAAATA------TAAGTAA--AA--AGAAGC---------GAAAAAGC-------C------------AGGATG-CGAGGGG-----------TAAAAACCAATTTAAGGTT-TTCCTTTTTGG-CGCCAAATTATCTTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAG----TGGGGCAGGTTG---------------------------------------------------------------------TGGGG-------------GAG--------------------GTG-G-----------GCC---AAA-GG------------GCGGTGCCAC-------	Size	Hit Count	Total Norm	Total
No Reads

droTak1

AGTTCAGCCCGGAGCCGCCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATAT------------------------------------------------------------CTCT------TAAAATCAATTTACAGC----------A----------------------------------------------------AAAAAAAGTTACT----AA--AAAAAG--A---A--------AAGAGC-----------------------GGGC-CAGGGG-----------GAAAAACCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCTTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAG----TGGAGCATGGGT------------------------------------------------------------------------GGC-------------AG--------------------GGG-G-----------CGT---AAG-AG------------GCGGGGCCAC-------	Size	Hit Count	Total Norm	Total
No Reads

droEug1

AGTTTAGCCCAGAGCCGCCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATAT------------------------------------------------------------CTCT------TAAAATCAATTTGCAGG----------CTAAAAG-------------------------------TA--ACC-------------------------AA--ATAA--AAAC---------GAAAAAGCGGCAAACC-------------TGCGGGCGAGG-GGGAA-----------GACCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCTTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAG----TGGAGCGCGTTT----------------------------------------------------------------------CGGTGAGGTG-GG-CAGATG--------------------GTGGG-----------GTTCAAAAG-AG------------GCGGGGCCAC-------	Size	Hit Count	Total Norm	Total
No Reads

dm3

AGTTCAGCCCGGAGCCACCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATAT------------------------------------------------------------CTCT------TAAAATCAATTTAC--------------TAAAAG-------------------------------TA--ACC-------------------------AA--AAAA--AAAAAAAAAAA--------G---CAAACAA------AAATTAAGCGGCCGCGG-GGCCA-----------TAGCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCGTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAG----TGGGG-----------------------------------------------------------------------------------GGCG-CG-A-GGAG--------------------GTAAG-----------GAT---AAA-GG------------GCGGGGCCATG------	Size	Hit Count	Total Norm	Total	GSM154618 Ovary	GSM154620 Ovary	GSM154621 Ovary	GSM154622 Ovary	GSM180328 Head	GSM180329 Mixed-body	GSM180330 Embryo	GSM180331 Embryo	GSM180332 Embryo	GSM180333 Embryo	GSM180334 Larvae	GSM180335 Disc	GSM180336 Pupae	GSM180337 S2	GSM231091 Ovary	GSM239041 Head	GSM239050 Head	GSM239051 S2	GSM239052 S2	GSM246084 Head	GSM266765 S2	GSM280082 Ovary	GSM280083 Ovary	GSM280084 Ovary	GSM280085 Testis	GSM280086 Ovary	GSM280087 S2-NP	GSM280088 S2-NP	GSM312995 Head	GSM313160 Head	GSM313161 Head	GSM313162 Head	GSM313163 Head	GSM313164 Head	GSM313165 Head	GSM343287 Embryo	GSM379050 Ovary	GSM379051 Ovary	GSM379052 Ovary	GSM379053 Ovary	GSM379054 Ovary	GSM379055 Ovary	GSM379056 Ovary	GSM379057 Ovary	GSM379058 Ovary	GSM379059 Ovary	GSM379060 Ovary	GSM379061 Ovary	GSM379062 Ovary	GSM379063 Ovary	GSM379064 Ovary	GSM379065 Ovary	GSM379066 Ovary	GSM379067 Ovary	GSM424739 S2	GSM424740 S2	GSM424741 S2	GSM467729 Female-body	GSM467730 Female-body	GSM467731 Female-body	M002 CS	M003 Male-body	M004 Male-body	M005 ML-DmD32	M006 ML-DmD32	M029 OSC	M030 OSC	M031 OSC	M032 S2-R+	M033 S2-R+	M034 S2-R+	M035 S2-R+	M036 S2-R+	M037 S2-R+	M038 S2-R+	M039 S2-R+	M048 S2-R+	OSS2 OSS	OSS6 OSS	OSS7 OSS	OSS8 OSS	S1 Head	S10 Embryo	S11 Embryo	S12 Embryo	S13 Embryo	S14 Embryo	S15 S2-GMR	S16 Kc	S17 Larvae	S18 Larvae	S19 Pupae	S2 Head	S20 Pupae	S21 Disc	S22 Head	S23 Female-body	S24 Male-body	S25 Larvae	S26 Larvae	S27 Pupae	S28 Pupae	S29 Head	S3 Male-body	S30 S2-GMR	S31 S2	S32 S2	S33 Kc	S34 Kc	S35 Kc	S4 Female-body	S5 Disc	S6 Embryo	S7 Embryo	S8 S2	S9 Embryo	SRR001337 Head	SRR001338 Head	SRR001339 Head	SRR001340 Head	SRR001341 Head	SRR001342 Head	SRR001343 Head	SRR001344 Head	SRR001345 Head	SRR001346 Head	SRR001347 Head	SRR001348 Head	SRR001349 Head	SRR001664 Head	SRR010951 Ovary	SRR010952 Ovary	SRR010953 Ovary	SRR010954 Ovary	SRR010955 Ovary	SRR010956 Ovary	SRR010957 Ovary	SRR010958 Ovary	SRR010959 Ovary	SRR010960 Ovary	SRR014268 Embryo	SRR014269 Embryo	SRR014270 Embryo	SRR014271 Embryo	SRR014272 Embryo	SRR014273 Ovary	SRR014274 Ovary	SRR014275 Ovary	SRR014276 Ovary	SRR014277 Ovary	SRR014278 Ovary	SRR014279 Ovary	SRR014280 Ovary	SRR014281 Ovary	SRR014282 Ovary	SRR023197 S2	SRR023399 S2	SRR023400 S2	SRR023402 S2	SRR023407 S2	SRR029028 S2	SRR029029 S2	SRR029030 S2	SRR029031 S2	SRR029032 S2	SRR029033 S2	SRR029608 Head	SRR029633 Head	SRR030145 gb3	SRR031692 Head	SRR031693 Head	SRR031694 Head	SRR031696 Head	SRR031697 Head	SRR031698 Head	SRR031699 Head	SRR031700 Head	SRR031701 Head	SRR031702 Head	SRR031703 Head	SRR031704 Head	SRR032092 S2	SRR032093 S2	SRR032094 S2	SRR032095 S2-NP	SRR032096 S2-NP	SRR032152 Head	SRR060643 Testis	SRR060644 Ovary	SRR060645 Testis	SRR060646 Ovary	SRR060647 Ovary	SRR060648 Ovary	SRR060649 Ovary	SRR060650 Testis	SRR060651 Ovary	SRR060652 Testis	SRR060653 Ovary	SRR065151 Ovary	SRR065152 Ovary	SRR065153 Ovary	SRR065154 Ovary	SRR065155 Ovary	SRR065800 Ovary	SRR065801 Ovary	SRR065802 Ovary	SRR065803 Ovary	SRR065804 Ovary	SRR065805 Ovary	SRR065806 Ovary	SRR065807 Ovary	SRR097865 S2-NP	SRR097866 S2-NP	SRR097867 S2-NP	SRR298536 Ovary	SRR298537 Ovary	SRR298711 Ovary	SRR298712 Ovary	SRR317110 Ovary	SRR317111 Ovary	SRR317112 Ovary	SRR317113 Ovary	SRR317114 Ovary	SRR317115 Ovary	SRR317116 Ovary	SRR317117 Ovary	SRR341115 S2	SRR341116 S2	SRR341117 S2	SRR341118 S2	SRR351332 Mixed-body	SRR351333 Mixed-body	T004 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CME-W2	V136 Male-body	V137 Head	V138 Male-body	V139 Female-body	V140 Female-body	V141 Female-body	V142 Female-body	V144 OSC	V145 S2	V146 S1	V147 1182-2H	V148 mbn2	GSM1528798 follicle cells
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.................................................................................................................................................................................................................................AA--AAAA--AAAAAAAAAAA--------G---CAA...................................................................................................................................................................................................................................................................................................................................................................	21	17	0.12	2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................................................................................................G-------------------------------TA--ACC-------------------------AA--AAAA--AAAAAAAAAAA--------..........................................................................................................................................................................................................................................................................................................................................................................	23	9	0.11	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................A--ACC-------------------------AA--AAAA--AAAAAAAAAAA--------G---......................................................................................................................................................................................................................................................................................................................................................................	22	9	0.11	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
..................................................................................................................................................................................................................................A--AAAA--AAAAAAAAAAA--------G---CAAA..................................................................................................................................................................................................................................................................................................................................................................	21	11	0.09	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.................................................................................................................................................................G-------------------------------TA--ACC-------------------------AA--AAAA--AAAAAAA......................................................................................................................................................................................................................................................................................................................................................................................	19	12	0.08	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
.....................................................................................................................................................................................................................................AAAA--AAAAAAAAAAA--------G---CAAA..................................................................................................................................................................................................................................................................................................................................................................	20	15	0.07	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

droSim2

AGTTCAGCCCGGAGCCACCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATAT------------------------------------------------------------CTCT------TAAAATCAATTTACAGG----------CTAAAAG-------------------------------TA--ACC-------------------------AA--CAAA--AA-----AAAA--------G---CAAACAA------AAATTAAGCGGCCGCGG-GGCAA-----------TAGCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCGTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAG----TGGAGCGTGTTG------------------------------------------------------------------------GTGGGGCG-CG-A-GGAG--------------------GTAAG-----------GAT---AAA-GG------------GCGGGGCCAC-------

Size

Hit Count

Total Norm

Total

M023
Head

M024
Male-body

M025
Embryo

M053
Female-body

O001
Testis

O002
Head

SRR553485
Ovary

SRR553486
Ovary

SRR553487
Ovary

SRR553488
Ovary

SRR618934
Ovary

V044
Embryo

SRR902008
Ovary

SRR902009
Testis

SRR1275487
Male larvae

SRR1275485
Male prepupae

SRR1275483
Male prepupae

No Reads

droSec2

AGTTCAGCCCGGAGCCACCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATAT------------------------------------------------------------CTCT------TAAAATCAATTTACAGG----------CTAAAAG-------------------------------TA--ACC-------------------------AA--CAAA--AAA---AAAAA--------G---CAAACAA------AAATTAAGCGGCCGCGG-GGCAA-----------TAGCAATTTAAGATT-TTCCTTTTTGG-TGCCAAATTATCGTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAG----TGGAGCGTGTGG-------------------------------------------------------------------------GGGGGTGGAG-A-GGAG--------------------GTAAG-----------GAT---AAA-GG------------GAGGGGCCAC-------	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
.............................................................................................................................................................................................................................................................................................................................................................................................................................................................GTGG-------------------------------------------------------------------------GGGGGTGGAG-A-GGAG--------------------.........................................................	19	1	1.00	1	0	0	1	0

droYak3

AGTTCAGCCCGGAGCCACCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATAT------------------------------------------------------------CTCT------TAAAATCAATTTACAGG----------CTAAAAG-------------------------------TA--ACCAGC--GCAAAAATA------CAA------AAAAA--AA---AAAGAACAAAAATGC-------CAAAAAACAAATTAAGCGGCCGAGG-GGCAA-----------TAGCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCGTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAG----TGGAGCGTGTGC------------------------------------------------------------------------GGGGGGTG-CG-G-GGAG--------------------GTAAG-----------GAT---AAA-GG------------GCGGTGCCAC-------	Size	Hit Count	Total Norm	Total	M026 Head	M043 Female-body	M056 Embryo	V046 Embryo	V052 Head	V058 Head	V120 Male-body	SRR1275488 Male larvae	SRR1275486 Male prepupae	SRR1275484 Male prepupae	GSM1528802 follicle cells
...............................................................................................................................................................................................................................................................................................................................................................................................................................................................GC------------------------------------------------------------------------GGGGGGTG-CG-G-GGAG--------------------GTA......................................................	20	1	1.00	1	0	0	1	0	0	0	0	0	0	0	0

droEre2

AGTTCAGCCCGGAGCCACCGACCCATATCTTCTGGTATCATCAGGATAAGGTAAATAT------------------------------------------------------------CTCT------CGAAATCAATTTACAGG----------CTAAGAG-------------------------------TA--ACC-------------------------AA--AAAA--AAGCAAAAAAA--------GCGAG----AA------AAATTAAGCGGCCGAGG--GCAA-----------TAGCAATTTAAGGTT-TTCCTCTTTGG-TGCCAAATTATCGTGCAATAATTGAGTTTG-TG--------------------------------CGCAGTAAAAAG---CGC------------AAAAG----TGGAGCGTGTGC------------------------------------------------------------------------GGGGGGTG-CG-G-GGCG--------------------GTAAG-----------GAT---AAA-GG------------GCGGAGCCAC-------	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
No Reads

dp5

AGTTCAGCCCAGAGCCGCCGACCCATATCTTTTGGTATCACCAGGACAAGGTAAGTAGCGCCGCCGCTCCACTCCGCGCCACTCCACTCTACAC----------CACAGCAAACCACTCCCT------TGAAATCAATTTACAGG----------G--AAAG-------------------------------CG--CCC-------------------------AC--AGAACGAAC---------------------------------------------------------------GAAAAACCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCTTGCAATAATTGAGTTTGGTG--------------------------------CGCCGTAAA-AG---CGC------------AA--G----TACA-------------------------------------------------------------------AGGGCAAG---------------------------GGCAAGAGGGATCGGGATCGG-----------GATCGA-GAAGGACGCCCCGCCCCGCCGTGCCAC-------

Size

Hit Count

Total Norm

Total

M022
Male-body

M040
Female-body

M059
Embryo

M062
Head

V043
Embryo

V051
Head

V112
Male-body

SRR902010
Ovary

SRR902011
Testis

SRR902012
CNS imaginal disc

No Reads

droPer2

AGTTCAGCCCAGAGCCGCCGACCCATATCTTTTGGTATCACCAGGACAAGGTAAGTAGCGCCGCTGCTACACTCCATGCCACTCCGCGCTACACCACACCACAGCACAGCAAACCACTCCCT------TGAAATCAATTTACAGG----------G--AAAG-------------------------------CG--CCC-------------------------AC--AGAACGAAC---------------------------------------------------------------GAAAAACCAATTTAAGGTT-TTCCTTTTTGG-TGCCAAATTATCTTGCAATAATTGAGTTTGGTG--------------------------------CGCCGTAAA-AG---CGC------------AA--G----TACA-------------------------------------------------------------------AGGGCAAG------GGC---------------AAGGGCAAGAGGGATCGGGATTGG-----------GATCGA-GAAGGACGCCCCGCCCCGCCGTGCCAC-------	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
No Reads

droWil2

AATTTAGTCCGGAGCCACCTACCCATATCTTTTGGTATCATCAGGATAAGGTAAATGTATACGA----------------------------AT----------CACAGTTCACAGTTC----------CCAATCAATTTTCATA----------CAGAGAG---------TGAGATGGTGGGGAGGGGGGGTTA------GCGAATGAAATTT------CAAGTACACAAAATGAAA---------------------------------------------------A-----------GAAAACCCAATTTAATGTT-TTCCTCTTTGGAAGCCAAATTATCTTGCAATAATTGAGTTTT-TTTCGGTATTTCTCTCCTCCTCCTCCGTTCTCTTAGCAGTTAAAAG---CAC------------AAAAA----AATA---------------------------------------------------------------------TATACATATATGAAGAGTG-GG-G-GATG--------------------GTACCAATGCAGGATAATC-GC-AG-AG------------TCTGAGCCATCGTCACC	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
No Reads

droVir3

AGTTCAGTCCGGAGCCACCAACACACATTTTCTGGTATCATCAGGATAAGGTGAGTAT------------------------------------------------------------CATACCAGAGTCAAATCAATTTATGA---------------GAGTAATAAAAT--AAATG---------------A-----------------------------------------------A--------ACAAG------------------TTTAAA--------------A-----------GATAAATAAAAAATT-TTGTTGTGTGG--ATCTAATTATCTTGCAATAATTGAGTTTTGCC--------------------------------CTCGCTAAAA-G---AAA-------AGTTAAAAAA---TCAGAGCAGC--CACGCCCACAGT---------------------------------------------------T------------------------------------------------------------------CAA----------------------------A--ACCC

Size

Hit Count

Total Norm

Total

M027
Male-body

M028
Head

M047
Female-body

M061
Embryo

SRR060654
Ovary

SRR060655
Testis

SRR060656
Ovary

SRR060657
Testis

SRR060658
Ovary

SRR060659
Testis

SRR060660
Ovary

SRR060661
Embryo

SRR060662
Embryo

SRR060663
Embryo

SRR060664
Male-body

SRR060665
Female-body

SRR060666
Male-body

SRR060667
Female-body

SRR060668
Male-body

SRR060669
Female-body

SRR060670
Testis

SRR060671
Male-body

SRR060672
Female-body

SRR060673
Ovary

SRR060674
Ovary

SRR060675
Ovary

SRR060676
Ovary

SRR060677
Ovary

SRR060678
Testis

SRR060679
Testis

SRR060680
Testis

SRR060681
Testis

SRR060682
Embryo

SRR060683
Testis

SRR060684
Embryo

SRR060685
Embryo

SRR060686
Embryo

SRR060687
Embryo

SRR060688
Ovary

SRR060689
Testis

SRR1106712
embryo_0-2h

SRR1106713
embryo_0-2h

SRR1106714
embryo_2-4h

SRR1106715
embryo_4-6h

SRR1106716
embryo_4-6h

SRR1106717
embryo_6-8h

SRR1106718
embryo_6-8h

SRR1106719
embryo_8-10h

SRR1106720
embryo_8-10h

SRR1106721
embryo_10-12h

SRR1106722
embryo_10-12h

SRR1106723
embryo_12-14h

SRR1106724
embryo_12-14h

SRR1106725
embryo_14-16h

SRR1106726
embryo_14-16h

SRR1106727
larvae

SRR1106728
larvae

SRR1106729
mixed whole adult body

SRR1106730
embryo_16-30h

V047
Embryo

V053
Head

V116
Male-body

GSM1528803
follicle cells

No Reads

droMoj3

AGTTCAGCCCGGAGCCGCCGACACACATCTTCTGGTATCACCAGGACAAGGTAAGC---------------------------------------------------------------------------AATCAATTTGTGA--------------A----------------------------------TA--TTC-------------------------AA--CATA--AGA---CGAAACCAAAAATGCTTT----AA----------------------------A-----------GATGCACTTAAAATT-C-CCGTGTGCG-CATCAAATTATCGTGGAATAATTGAACTTTGCC--------------------------------CTCTCTGAGAAC----TC------------GG--G----CA-----------------------------------CTCAAACTCAAACTGTTTCTCATACCAATACTTAAGCT------------------------------------------------------------------CAA----------------------------G--GCCC	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
No Reads

droGri2

AATTTAGTCCGGAGCCACCAACACACATTTTCTGGTATCATCAGGATAAGGTGAGCAT------------------------------------------------------------CGTATCAGAATTCAATCAATTTATGA---------------AAA-------------------------------TG--ACG-------------------------GG--AAAG--AAA---A--------AAAAG---------------------------TCGCTT-TGT-A-----------TATAAATTTAAATTT-TTC--TTTGCG-CCTCAAATTATCTTGCAATAATTGAGTTTTGCC--------------------------------CTCG-----------CACAAAAGGGCGCTGAAAAA---TCAGAGAATC--CACACCCACGATTCACAGCACATGCACTCAAACTCAGAGTGAAACTCATACTCAT---------------------------------------------------------------------------------------------ACCGTAGCACAAG-------	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

dan_2654

scaffold_13337:15054779-15054929 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads