ID:

dan_2546

 Coordinate:

scaffold_13337:10330914-10330968 -

 Confidence:

candidate

 Class:

Mirtron

 Genomic Locale:

intron

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-9.1 -9.1 -9.1

Flybase annnotation

intron [Dana\GF24177-in]; CDS [Dana\GF24177-cds]; utr5 [utr5_plus_8293]; CDS [Dana\GF24177-cds]

No Repeatable elements found

Sense Strand Reads

GAACGGAGCCAGAGAAGCCAAGCACACAAATGGAGTTAGTTTGGAAGGCGGTAAGGATAATTGATAAATTCTCGTTCTCAAGAAAATGACTCAATTCCTTTACAGTTTCTGCTATTATGGGTGATCCTGTATCTGCACACAGCCAACGGCCTCGA

**************************************************((((((..((((((...(((.((........)).)))...)))))).))))))..************************************************** 		Read size # Mismatch Hit Count Total Norm Total V106

head

 M044

female body

 V105

male body		 V055

head

 V039

embryo
.....................................................................................ATGACTCAATTCCTTTACAGT................................................. 21 0 1 20.00 20 12 4 3 1 0
.....................................................................................ATGACTCAATTCCTTTACAGTT................................................ 22 0 1 2.00 2 1 1 0 0 0
...................................................................................AAATGACTCAATTCCTTTACAG.................................................. 22 0 1 2.00 2 0 2 0 0 0
......................................................................................TGACTCAATTCCTTTACAGT................................................. 20 0 1 1.00 1 0 1 0 0 0
...................................................................................AAATGACTCAATTCCTTTACAGT................................................. 23 0 1 1.00 1 0 1 0 0 0
.....................................................................................ATTACTCAATTCCTTTACAGT................................................. 21 1 1 1.00 1 1 0 0 0 0
..................................................GTAAGGATAATTGATAAATTC.................................................................................... 21 0 1 1.00 1 1 0 0 0 0
.....................................................................................ATGACTCAATTCCTTTACAG.................................................. 20 0 1 1.00 1 0 1 0 0 0
........................................................................................................................GTGATCCTGTGTCTGCAGCCAG............. 22 3 2 0.50 1 0 0 0 1 0
......................................................................................................................................ACACCCAGCGAACGGCCTCG. 20 3 3 0.33 1 1 0 0 0 0
..................CAAACACACAATTGTAGTTAGT................................................................................................................... 22 3 3 0.33 1 0 0 0 0 1
...............................GGAGATACTTTTGAAGGCGGT....................................................................................................... 21 3 6 0.17 1 0 0 0 1 0
................................................................................................................ATTTATGGGTGATCCTGT......................... 18 2 8 0.13 1 0 0 0 1 0
......AGCTAGAGGAGCCAAGCA................................................................................................................................... 18 2 11 0.09 1 0 0 0 1 0
.....................................................................................................................................TGAACACAGCCGGCGGCCTC.. 20 3 12 0.08 1 0 0 0 1 0
.................................................................................................................ATTATGGATGATCCTGCATGT..................... 21 3 16 0.06 1 0 0 0 1 0
......................CACACCATTGGAGTTGGTTT................................................................................................................. 20 3 20 0.05 1 0 0 1 0 0
....................................................................................................................ATGGGAGATCCGGTATCGG.................... 19 3 20 0.05 1 0 0 0 1 0

Anti-sense strand reads

CTTGCCTCGGTCTCTTCGGTTCGTGTGTTTACCTCAATCAAACCTTCCGCCATTCCTATTAACTATTTAAGAGCAAGAGTTCTTTTACTGAGTTAAGGAAATGTCAAAGACGATAATACCCACTAGGACATAGACGTGTGTCGGTTGCCGGAGCT

**************************************************((((((..((((((...(((.((........)).)))...)))))).))))))..************************************************** 		Read size # Mismatch Hit Count Total Norm Total M058

embryo

 V039

embryo

 V055

head
.....................................................................................................................ACCCACTAGGACATCGATAT.................. 20 3 7 0.14 1 1 0 0
...................................................................................................................AAACCCACTAGGTCATATAC.................... 20 3 8 0.13 1 0 1 0
.......................TGTGGGTCCCTCAATCAAA................................................................................................................. 19 3 19 0.05 1 0 0 1

Re-alignment of all predicted orthologs

Species Coordinate ID Type Alignment
droAna3 scaffold_13337:10330864-10331018 - dan_2546 candidate GAACGGAGCCAGAGAAGCCAAGCAC-ACAAATGGAGTTAGTTTGGAAGGCGGTAAGGATAATTGATAAATTCTCGTTCTCAAGAAAATGACTCAATTCCTTTACAGTTTCTGCTATTATGGGTGATCCTGTATCTGCACACAGCCAACGGCCTCGA
droBip1 scf7180000396569:1039718-1039872 + CTACGAAGTCCCAGAAGCCAAGCAC-ACAAATGGAGCTAGTTTGGAAAGCGGTAAGGATAATTGAAAAATTCTCATTCCCAAGAAAATGACTCAATTCCTTTACAGTTTTTGCTATTTTGGGTGATCCTGTATCTGCACACAGTCAAAGGCCTCGA
droFic1 scf7180000453839:1687838-1687982 + CATTCGAGCCTCTATTGC-AAGCACACCAAATGGATTTTCTTTCGAAAGCGGTAAGAATAAATTTGAAA-------CCCTTTGGTAAATCTTTAATTCCTGTCCAGATATTCCTCTT---CCTGTTCGTATATCTTCACCTAACCAGCGGATATGT
droRho1 scf7180000758913:1088-1233 + GATTTGAGCCTCTAGAGT-AAGCACTACAAATGGATTTCCCGTGGAAAGCGGTAAGTAGCAATGTGAAAG------TCCTTTGGAAACTATCTAATTTCTGTACAGATGTTGTTCTT---ACTCTTCATGTATTTCCACTGCACCAGCGGATATGT
droBia1 scf7180000302428:3814106-3814244 - GAACG---CCT---TGGC-AAGCACTGAAAATGGAATTCCTGATGAAAGCGGTAAGAATAGATGAAAGA-------TCTTCGGGGAACTAACTAATTCCCGTGCAGCTTTTGTTTTT---CCTTTTCCTAACCTACCACTGCACCAGCGGATATGT
droTak1 scf7180000414337:6981-7123 - GAATCG--TCT---TTGC-TAGAACTACAAATGGATTTCCTATTGAAAGCGGTAAGAATAAATACGAAATAA----TCCCTTGGGAACTAATTAATTCCTGTGTAGCTTTTCTTTTT---CCTTTTCCTGTCCTTTCATTGCACCAGCGGATATGT

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droAna3
GAACGGAGCCAGAGAAGCCAAGCAC-ACAAATGGAGTTAGTTTGGAAGGCGGTAAGGATAATTGATAAATTCTCGTTCTCAAGAAAATGACTCAATTCCTTTACAGTTTCTGCTATTATGGGTGATCCTGTATCTGCACACAGCCAACGGCCTCGASizeHit CountTotal NormTotalM044

Female-body		M058

Embryo		V039

Embryo		V055

Head		V105

Male-body		V106

Head
......................................................................................ATGACTCAATTCCTTTACAGT.................................................21120.00204001312
......................................................................................ATGACTCAATTCCTTTACAGTT................................................2212.002100001
....................................................................................AAATGACTCAATTCCTTTACAG..................................................2212.002200000
.......................................................................................TGACTCAATTCCTTTACAGT.................................................2011.001100000
....................................................................................AAATGACTCAATTCCTTTACAGT.................................................2311.001100000
...................................................GTAAGGATAATTGATAAATTC....................................................................................2111.001000001
......................................................................................ATGACTCAATTCCTTTACAG..................................................2011.001100000
droBip1
CTACGAAGTCCCAGAAGCCAAGCAC-ACAAATGGAGCTAGTTTGGAAAGCGGTAAGGATAATTGAAAAATTCTCATTCCCAAGAAAATGACTCAATTCCTTTACAGTTTTTGCTATTTTGGGTGATCCTGTATCTGCACACAGTCAAAGGCCTCGASizeHit CountTotal NormTotal
No Reads
droFic1
CATTCGAGCCTCTATTGC-AAGCACACCAAATGGATTTTCTTTCGAAAGCGGTAAGAATAAATTTGAAA-------CCCTTTGGTAAATCTTTAATTCCTGTCCAGATATTCCTCTT---CCTGTTCGTATATCTTCACCTAACCAGCGGATATGTSizeHit CountTotal NormTotal
No Reads
droRho1
GATTTGAGCCTCTAGAGT-AAGCACTACAAATGGATTTCCCGTGGAAAGCGGTAAGTAGCAATGTGAAAG------TCCTTTGGAAACTATCTAATTTCTGTACAGATGTTGTTCTT---ACTCTTCATGTATTTCCACTGCACCAGCGGATATGTSizeHit CountTotal NormTotal
No Reads
droBia1
GAACG---CCT---TGGC-AAGCACTGAAAATGGAATTCCTGATGAAAGCGGTAAGAATAGATGAAAGA-------TCTTCGGGGAACTAACTAATTCCCGTGCAGCTTTTGTTTTT---CCTTTTCCTAACCTACCACTGCACCAGCGGATATGTSizeHit CountTotal NormTotal
No Reads
droTak1
GAATCG--TCT---TTGC-TAGAACTACAAATGGATTTCCTATTGAAAGCGGTAAGAATAAATACGAAATAA----TCCCTTGGGAACTAATTAATTCCTGTGTAGCTTTTCTTTTT---CCTTTTCCTGTCCTTTCATTGCACCAGCGGATATGTSizeHit CountTotal NormTotal
No Reads

Generated: 10/20/2015 at 07:05 PM