dan_2173

CDS [Dana\GF21739-cds]; exon [dana_GLEANR_5646:4]; CDS [Dana\GF21739-cds]; exon [dana_GLEANR_5646:3]; intron [Dana\GF21739-in]

Sense Strand Reads

##################################################------------------------------------------------------------------------------------------------------------------################################################## CCGACGATATAATCGAGATCTTCATCCTTGTCTCACTCATATTCGTGCAGGTAACCCACTCAATTACATCCCATCAAAAGGAAAACATTTTTCGTTTGGTATCCGCAGGGATACCAACAAAAAAACAAAAAAAAAAACCAAAACAACGCCCACCACCCACACAGATCCTGGTCAGCATCGCTGGTTGGTGGCTAACGTTTCGACTTCGTCAGCA ************************************************..................(((........))).....(((((.(((.(((((((....)))))))))).)))))........................................************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M058 embryo	V039 embryo	V055 head
..............................................................................................................................AAAAAAAAAAACCAGAACAAC...................................................................	21	1	1	1.00	1	1	0	0
...................................................................................................................................AAAAAACCAAAACAAGGCGCACC............................................................	23	2	1	1.00	1	1	0	0
.....................................................................................................................................AAAACCAAAACCACACCCACCAC..........................................................	23	2	1	1.00	1	1	0	0
................................................................................................................................AAAAAAGAACCAAAACATCGCCC...............................................................	23	2	1	1.00	1	1	0	0
...............................................................................................................................AAAAAAAAAACCAAAACAAC...................................................................	20	0	2	0.50	1	1	0	0
........................................................................................................................................ACCAAAACTGCGCCCACCA...........................................................	19	2	2	0.50	1	1	0	0
.......................................................................................................................AAAATACAAAAAAAAAAACCAAAACA.....................................................................	26	1	2	0.50	1	1	0	0
............................................................................................................................................AAACAACCCCCTCCACCCACCCA...................................................	23	3	2	0.50	1	1	0	0
.............................................................................................GTTTAGTATCCACAGGGA.......................................................................................................	18	2	2	0.50	1	0	0	1
...................................................................................................................AACAAAAACACAAAAAAAAAAACC...........................................................................	24	1	2	0.50	1	1	0	0
........................................................................................................................................AACAAAACAACGCGCACCAC..........................................................	20	2	2	0.50	1	1	0	0
.................................................................................................................................AAAAAAAACCACAACAAAGACCACC............................................................	25	3	2	0.50	1	1	0	0
...........................................................................................................................................AAAACAAGGCCCACCACC.........................................................	18	1	2	0.50	1	1	0	0
.....................................................................................................................................AAAACAAAAACAACGGCCCCCAC..........................................................	23	3	3	0.33	1	0	1	0
...................................................................................................................................AAAAAACAAAAACTACCCCCACCA...........................................................	24	3	3	0.33	1	1	0	0
.......................................................................................................................................AACCAAAACACCCCCCACCA...........................................................	20	2	3	0.33	1	0	1	0
.............................................................................................GGTTGGTAACCGCAGGGA.......................................................................................................	18	2	3	0.33	1	0	0	1
......................................................................................................................AAAAAAACAAAAAAAAAAACC...........................................................................	21	0	6	0.33	2	2	0	0
................................................................................................................ACCAACAAAAAAACAAAAAAA.................................................................................	21	0	3	0.33	1	1	0	0
..................................................................................................................................AAAAAAACCAAAACCACTACCACCA...........................................................	25	3	3	0.33	1	1	0	0
.......................................................................................................................................AACCAAGACAAAACCCACCACC.........................................................	22	3	3	0.33	1	1	0	0
.....................................................................................................................................AGAACCAAAACACTGCCCACC............................................................	21	3	4	0.25	1	1	0	0
.......................................................................................................................AAAAAACAAAAAAAAAAACC...........................................................................	20	0	12	0.25	3	3	0	0
....................................................................................................................................AAAAACCAAATCAACCACCACCA...........................................................	23	3	4	0.25	1	1	0	0
......................................................................................................................................AAACCAAAACGGAGCCCACCAC..........................................................	22	3	4	0.25	1	1	0	0
...................................................................................................................................AAAAAACCAAAACAAAGCCC...............................................................	20	1	4	0.25	1	1	0	0
....................................................................................................................................AAAAACCAAAACGAAACCCACCAC..........................................................	24	3	4	0.25	1	1	0	0
.......................................................................................................................AAAAAACAAAAAAAGAAACCAAAACCA....................................................................	27	2	5	0.20	1	1	0	0
.....................................................................................................................................AAAACAAAAACAACGACCACC............................................................	21	2	6	0.17	1	1	0	0
.........................................................................................................................AAAACAAAAAAAAAAACCAAAA.......................................................................	22	0	7	0.14	1	1	0	0
....................................................................................................................................AAAAAACAAAACAACCTCCACCA...........................................................	23	3	7	0.14	1	1	0	0
.........................................................................................................................AAAACAAAAAAAAAAACCAAA........................................................................	21	0	8	0.13	1	1	0	0
......................................................................................................................AAAAAAACAAAAAACAAAACCAAAAGA.....................................................................	27	2	8	0.13	1	1	0	0
...............................................................................................................................AAAAAAAAAACCAAAACA.....................................................................	18	0	17	0.12	2	1	1	0
................................................................................................................................AAAAAGAACCCAAAACCACGCC................................................................	22	3	9	0.11	1	1	0	0
.......................................................................................................................AAAAAACAAAAAACAAAACCACAAC......................................................................	25	2	10	0.10	1	1	0	0
......................................................................................................................AAAAAAACAAAAGAAAAAACCAAA........................................................................	24	1	10	0.10	1	1	0	0
.........................................................................................................................AAAACAAAAAAAAAAACC...........................................................................	18	0	20	0.10	2	2	0	0
......................................................................................................................................GCACCAAAACAACTCCCACCA...........................................................	21	3	10	0.10	1	0	1	0
...........................................................................................................................AACAAAAAAAAAAACCAAACC......................................................................	21	1	10	0.10	1	1	0	0
..................................................................................................................CAACACAAAAACAAAAAAACAAACCC..........................................................................	26	3	11	0.09	1	1	0	0
......................................................................................................................................AAAACAAAACTACTCCCACCA...........................................................	21	3	13	0.08	1	1	0	0
........................................................................................................................AAAAACAAAAAAAAAAACCA..........................................................................	20	0	13	0.08	1	1	0	0
............................................................................................................................................AAAGAAAGCCCACCACCCA.......................................................	19	2	13	0.08	1	1	0	0
.........................................................................................................................AAAACAAAAAAAAAAACCA..........................................................................	19	0	13	0.08	1	1	0	0
...................................................................................................................AACAAAACAACTAAAAGAAAAACC...........................................................................	24	3	13	0.08	1	1	0	0
...........................................................................................................................AACAAAAAACAAGACCAAAAC......................................................................	21	2	13	0.08	1	0	1	0
.....................................................................................................................................AAAAACAAAAGATCGCCCACC............................................................	21	3	14	0.07	1	1	0	0
....................................................................................................................ACAAAAAAACACAAAAAAAAAC............................................................................	22	1	14	0.07	1	1	0	0
................................................................................................................ACCAACAAAAAACCAAAAAA..................................................................................	20	1	15	0.07	1	1	0	0
......................................................................................................................AAAAAAACAAAAAAAAGAACC...........................................................................	21	1	15	0.07	1	1	0	0
..................................................................................................................CTAGAAAAAAACAAAAAACAAAACC...........................................................................	25	3	16	0.06	1	1	0	0
...................................................................................................................................................................................GCTGGTTGATGGGTAAC..................	17	2	17	0.06	1	0	0	1
...........................................................................................................................AACAAAAAAAAAAACCGAACCAA....................................................................	23	2	17	0.06	1	0	1	0
............................................................................................................................ACAAAAAAAAAAACCAAAAGA.....................................................................	21	1	19	0.05	1	1	0	0
....................................................................................................................ACAAAAAACCAAAAAAAAAAACCT..........................................................................	24	2	19	0.05	1	1	0	0
.........................................................................................................................AAAACAAAAAAGAAAACCAAAA.......................................................................	22	1	19	0.05	1	1	0	0
...........................................................................................................................AACAAAAAAACAAACCAAGAC......................................................................	21	2	19	0.05	1	1	0	0
...................................................................................................................................AAAAAACTAAAACAACACCC...............................................................	20	2	19	0.05	1	1	0	0
.........................................................................................................................AAAACAAAAAAAAAAACCAGAA.......................................................................	22	1	19	0.05	1	1	0	0
......................................................................................................................AAAAAAACCAAAAAAAACACCA..........................................................................	22	2	20	0.05	1	1	0	0
.........................................................................................................................AAAAGAAAAAAAAAAACCAA.........................................................................	20	1	20	0.05	1	1	0	0
........................................................................................................................................ACCAAAATAAAGACCACCAC..........................................................	20	3	20	0.05	1	1	0	0
......................................................................................................................AAAACAACAAAAAAAAGAACCA..........................................................................	22	2	20	0.05	1	1	0	0
......................................................................................................................................AAAACAAAAGAACGACCACCA...........................................................	21	3	20	0.05	1	0	1	0
................................................................................................................................AAAAAAAAAACAAAACAACG..................................................................	20	1	20	0.05	1	0	1	0
.......................................................................................................................AAACAACAAAAAAAAAAACC...........................................................................	20	1	20	0.05	1	1	0	0
......................................................................................................................AAAAACACAAAAAAAAAAACC...........................................................................	21	1	20	0.05	1	1	0	0
......................................................................................................................AAAAAAACGAAAAAAAAAACC...........................................................................	21	1	20	0.05	1	1	0	0
....................................................................................................................ACAAAAAAACGAAAAAAAGAACCC..........................................................................	24	3	20	0.05	1	1	0	0
.......................................................................................................................AACAAACAAAAAAAAAAACC...........................................................................	20	1	20	0.05	1	1	0	0
......................................................................................................................AATAAAACAAAAAAAAAAACC...........................................................................	21	1	20	0.05	1	1	0	0
...........................................................................................................................................AAAAGATTGCCCACCACCCA.......................................................	20	3	20	0.05	1	1	0	0
................................................................................................................................AAAAGAAAACCAGAACAAC...................................................................	19	2	20	0.05	1	1	0	0
.......................................................................................................................AAAAAACAAAAAAAGAAACC...........................................................................	20	1	20	0.05	1	1	0	0
......................................................................................................................AACAAAACAAAAAAAAAAACC...........................................................................	21	1	20	0.05	1	1	0	0
...........................................................................................................................AACAACAAAAAAAACCAAAA.......................................................................	20	1	20	0.05	1	1	0	0
........................................................................................................................GAAAACAAAAAAAAAAACCA..........................................................................	20	1	20	0.05	1	1	0	0
.........................................................................................TTTCGTTTGGTCTCCGGACG.........................................................................................................	20	3	20	0.05	1	0	0	1
.......................................................................................................................................AAAAAAAAGAACGCCCACCAC..........................................................	21	3	20	0.05	1	1	0	0

##################################################------------------------------------------------------------------------------------------------------------------##################################################
CCGACGATATAATCGAGATCTTCATCCTTGTCTCACTCATATTCGTGCAGGTAACCCACTCAATTACATCCCATCAAAAGGAAAACATTTTTCGTTTGGTATCCGCAGGGATACCAACAAAAAAACAAAAAAAAAAACCAAAACAACGCCCACCACCCACACAGATCCTGGTCAGCATCGCTGGTTGGTGGCTAACGTTTCGACTTCGTCAGCA
**************************************************..................(((........))).....(((((.(((.(((((((....)))))))))).)))))........................................**************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M058
embryo

V039
embryo

V055
head

..............................................................................................................................AAAAAAAAAAACCAGAACAAC...................................................................

1.00

...................................................................................................................................AAAAAACCAAAACAAGGCGCACC............................................................

1.00

.....................................................................................................................................AAAACCAAAACCACACCCACCAC..........................................................

1.00

................................................................................................................................AAAAAAGAACCAAAACATCGCCC...............................................................

1.00

...............................................................................................................................AAAAAAAAAACCAAAACAAC...................................................................

0.50

........................................................................................................................................ACCAAAACTGCGCCCACCA...........................................................

0.50

.......................................................................................................................AAAATACAAAAAAAAAAACCAAAACA.....................................................................

0.50

............................................................................................................................................AAACAACCCCCTCCACCCACCCA...................................................

0.50

.............................................................................................GTTTAGTATCCACAGGGA.......................................................................................................

0.50

...................................................................................................................AACAAAAACACAAAAAAAAAAACC...........................................................................

0.50

........................................................................................................................................AACAAAACAACGCGCACCAC..........................................................

0.50

.................................................................................................................................AAAAAAAACCACAACAAAGACCACC............................................................

0.50

...........................................................................................................................................AAAACAAGGCCCACCACC.........................................................

0.50

.....................................................................................................................................AAAACAAAAACAACGGCCCCCAC..........................................................

0.33

...................................................................................................................................AAAAAACAAAAACTACCCCCACCA...........................................................

0.33

.......................................................................................................................................AACCAAAACACCCCCCACCA...........................................................

0.33

.............................................................................................GGTTGGTAACCGCAGGGA.......................................................................................................

0.33

......................................................................................................................AAAAAAACAAAAAAAAAAACC...........................................................................

0.33

................................................................................................................ACCAACAAAAAAACAAAAAAA.................................................................................

0.33

..................................................................................................................................AAAAAAACCAAAACCACTACCACCA...........................................................

0.33

.......................................................................................................................................AACCAAGACAAAACCCACCACC.........................................................

0.33

.....................................................................................................................................AGAACCAAAACACTGCCCACC............................................................

0.25

.......................................................................................................................AAAAAACAAAAAAAAAAACC...........................................................................

0.25

....................................................................................................................................AAAAACCAAATCAACCACCACCA...........................................................

0.25

......................................................................................................................................AAACCAAAACGGAGCCCACCAC..........................................................

0.25

...................................................................................................................................AAAAAACCAAAACAAAGCCC...............................................................

0.25

....................................................................................................................................AAAAACCAAAACGAAACCCACCAC..........................................................

0.25

.......................................................................................................................AAAAAACAAAAAAAGAAACCAAAACCA....................................................................

0.20

.....................................................................................................................................AAAACAAAAACAACGACCACC............................................................

0.17

.........................................................................................................................AAAACAAAAAAAAAAACCAAAA.......................................................................

0.14

....................................................................................................................................AAAAAACAAAACAACCTCCACCA...........................................................

0.14

.........................................................................................................................AAAACAAAAAAAAAAACCAAA........................................................................

0.13

......................................................................................................................AAAAAAACAAAAAACAAAACCAAAAGA.....................................................................

0.13

...............................................................................................................................AAAAAAAAAACCAAAACA.....................................................................

0.12

................................................................................................................................AAAAAGAACCCAAAACCACGCC................................................................

0.11

.......................................................................................................................AAAAAACAAAAAACAAAACCACAAC......................................................................

0.10

......................................................................................................................AAAAAAACAAAAGAAAAAACCAAA........................................................................

0.10

.........................................................................................................................AAAACAAAAAAAAAAACC...........................................................................

0.10

......................................................................................................................................GCACCAAAACAACTCCCACCA...........................................................

0.10

...........................................................................................................................AACAAAAAAAAAAACCAAACC......................................................................

0.10

..................................................................................................................CAACACAAAAACAAAAAAACAAACCC..........................................................................

0.09

......................................................................................................................................AAAACAAAACTACTCCCACCA...........................................................

0.08

........................................................................................................................AAAAACAAAAAAAAAAACCA..........................................................................

0.08

............................................................................................................................................AAAGAAAGCCCACCACCCA.......................................................

0.08

.........................................................................................................................AAAACAAAAAAAAAAACCA..........................................................................

0.08

...................................................................................................................AACAAAACAACTAAAAGAAAAACC...........................................................................

0.08

...........................................................................................................................AACAAAAAACAAGACCAAAAC......................................................................

0.08

.....................................................................................................................................AAAAACAAAAGATCGCCCACC............................................................

0.07

....................................................................................................................ACAAAAAAACACAAAAAAAAAC............................................................................

0.07

................................................................................................................ACCAACAAAAAACCAAAAAA..................................................................................

0.07

......................................................................................................................AAAAAAACAAAAAAAAGAACC...........................................................................

0.07

..................................................................................................................CTAGAAAAAAACAAAAAACAAAACC...........................................................................

0.06

...................................................................................................................................................................................GCTGGTTGATGGGTAAC..................

0.06

...........................................................................................................................AACAAAAAAAAAAACCGAACCAA....................................................................

0.06

............................................................................................................................ACAAAAAAAAAAACCAAAAGA.....................................................................

0.05

....................................................................................................................ACAAAAAACCAAAAAAAAAAACCT..........................................................................

0.05

.........................................................................................................................AAAACAAAAAAGAAAACCAAAA.......................................................................

0.05

...........................................................................................................................AACAAAAAAACAAACCAAGAC......................................................................

0.05

...................................................................................................................................AAAAAACTAAAACAACACCC...............................................................

0.05

.........................................................................................................................AAAACAAAAAAAAAAACCAGAA.......................................................................

0.05

......................................................................................................................AAAAAAACCAAAAAAAACACCA..........................................................................

0.05

.........................................................................................................................AAAAGAAAAAAAAAAACCAA.........................................................................

0.05

........................................................................................................................................ACCAAAATAAAGACCACCAC..........................................................

0.05

......................................................................................................................AAAACAACAAAAAAAAGAACCA..........................................................................

0.05

......................................................................................................................................AAAACAAAAGAACGACCACCA...........................................................

0.05

................................................................................................................................AAAAAAAAAACAAAACAACG..................................................................

0.05

.......................................................................................................................AAACAACAAAAAAAAAAACC...........................................................................

0.05

......................................................................................................................AAAAACACAAAAAAAAAAACC...........................................................................

0.05

......................................................................................................................AAAAAAACGAAAAAAAAAACC...........................................................................

0.05

....................................................................................................................ACAAAAAAACGAAAAAAAGAACCC..........................................................................

0.05

.......................................................................................................................AACAAACAAAAAAAAAAACC...........................................................................

0.05

......................................................................................................................AATAAAACAAAAAAAAAAACC...........................................................................

0.05

...........................................................................................................................................AAAAGATTGCCCACCACCCA.......................................................

0.05

................................................................................................................................AAAAGAAAACCAGAACAAC...................................................................

0.05

.......................................................................................................................AAAAAACAAAAAAAGAAACC...........................................................................

0.05

......................................................................................................................AACAAAACAAAAAAAAAAACC...........................................................................

0.05

...........................................................................................................................AACAACAAAAAAAACCAAAA.......................................................................

0.05

........................................................................................................................GAAAACAAAAAAAAAAACCA..........................................................................

0.05

.........................................................................................TTTCGTTTGGTCTCCGGACG.........................................................................................................

0.05

.......................................................................................................................................AAAAAAAAGAACGCCCACCAC..........................................................

0.05

Anti-sense strand reads

GGCTGCTATATTAGCTCTAGAAGTAGGAACAGAGTGAGTATAAGCACGTCCATTGGGTGAGTTAATGTAGGGTAGTTTTCCTTTTGTAAAAAGCAAACCATAGGCGTCCCTATGGTTGTTTTTTTGTTTTTTTTTTTGGTTTTGTTGCGGGTGGTGGGTGTGTCTAGGACCAGTCGTAGCGACCAACCACCGATTGCAAAGCTGAAGCAGTCGT ************************************************..................(((........))).....(((((.(((.(((((((....)))))))))).)))))........................................************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V055 head	M044 female body	V106 head
.............................................................................................................................................................................TCATAGCAACCAACCACAGAT....................	21	3	2	0.50	1	1	0	0
..............................................................................................................................................................................................CGATTGCTAGGCTGAAGGAGT...	21	3	3	0.33	1	1	0	0
...............................................................................................................................................GGTACGGGTGGTGGGTGT.....................................................	18	2	20	0.30	6	0	6	0
...............................................................................................................................................GGTACGGGTGGTGGGTGTTTC..................................................	21	3	8	0.13	1	0	1	0
....................................................TTGGGTGAGTTGATGGAGG...............................................................................................................................................	19	2	9	0.11	1	0	1	0
................................................................................................................................................ATGCGTGTGGTGGGTGTG....................................................	18	2	20	0.10	2	0	2	0
........................................AAAGCACGGCTATTGGGTG...........................................................................................................................................................	19	3	20	0.05	1	1	0	0
.....................................................................................................................................................GGTGGTGGGTGTGTGTAGGT.............................................	20	2	20	0.05	1	0	0	1

GGCTGCTATATTAGCTCTAGAAGTAGGAACAGAGTGAGTATAAGCACGTCCATTGGGTGAGTTAATGTAGGGTAGTTTTCCTTTTGTAAAAAGCAAACCATAGGCGTCCCTATGGTTGTTTTTTTGTTTTTTTTTTTGGTTTTGTTGCGGGTGGTGGGTGTGTCTAGGACCAGTCGTAGCGACCAACCACCGATTGCAAAGCTGAAGCAGTCGT
**************************************************..................(((........))).....(((((.(((.(((((((....)))))))))).)))))........................................**************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

V055
head

M044
female body

V106
head

0.50

0.33

...............................................................................................................................................GGTACGGGTGGTGGGTGT.....................................................

0.30

...............................................................................................................................................GGTACGGGTGGTGGGTGTTTC..................................................

0.13

....................................................TTGGGTGAGTTGATGGAGG...............................................................................................................................................

0.11

................................................................................................................................................ATGCGTGTGGTGGGTGTG....................................................

0.10

........................................AAAGCACGGCTATTGGGTG...........................................................................................................................................................

0.05

.....................................................................................................................................................GGTGGTGGGTGTGTGTAGGT.............................................

0.05

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droAna3	scaffold_13334:809579-809792 -	dan_2173	CCGACGATATA--------------------------------ATCGAGATCTTCATCCTTGTCTCACTCATATTCGTGCAGGTA--ACCCACTCAATTACATCCCATCAAAAGGAAAACATTTTTCGTTTG---------------GTATCCGCAGGGATACCAACAAAAAAACAAAAAAAAAAACCAAAACAACGCCCACCACCCACACAGATCCTGGTCAGCATCGCTGGTTGGTGGCTAACGTTTCGACTTCGTCAGCA
droBip1	scf7180000396733:443815-444011 -		CCGAGGATGTC--------------------------------ATCGAAATATGCATTCTTGTTATTCTTATAGTCGTGCAGGTA--ACCCACTCAATTATATCCAATCGAAAGAAAAACATTATTCGTTTG---------------GTATCCGCAGCGATACCAACAAA--------------AACCA--ACAACGCCCACC-CACACACAGATCCTAGTCAGCATCGCTGGTTGGTGGCTAACGTATCGACTTCATCGCCA
dp5	XL_group1e:1075057-1075266 +		ATGGCAGGGTGGACAACATAATCATGAAACTTTAGATGAAGGCATTGGCGATGCTATGCTTTTGGTTATCAATATTGTGCAGGTGCGATTCAATTAATCAT-TCACATCA----------ATCATTTGTATGAAAATACGATACGATGCATCTACATAGGT------------------------------------------AGCCATTCAGGTTTCTGTCAATATTGCCGGCTGGTGGCTGGCCGTTCGACTGCGGAGGCA
droPer2	scaffold_30:121122-121331 -		ATGGCAGGGTGGACAACATAATCATGAAACTTTAGATGAAGGCATTGGCGATGCTATGCTTTTGGTTATCAATATTGTGCAGGTGCGATTCAATTAATCAT-TCACATCA----------ATCATTTGTATGAAAATACGATACGATGCATCTACATAGGT------------------------------------------AGCCATTCAGGTTTCTGTCAATATTGCCGGCTGGTGGCTGGCCGTTCGACTGCGGAGGCA
droWil2	scf2_1100000004909:6179906-6179958 +		C------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGGTGGTGGTCAACATTGCTGGCTGGTGGCTAGCTATGCGTTTGCGTAAATA

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droAna3

CCGACGATATA--------------------------------ATCGAGATCTTCATCCTTGTCTCACTCATATTCGTGCAGGTA--ACCCACTCAATTACATCCCATCAAAAGGAAAACATTTTTCGTTTG---------------GTATCCGCAGGGATACCAACAAAAAAACAAAAAAAAAAACCAAAACAACGCCCACCACCCACACAGATCCTGGTCAGCATCGCTGGTTGGTGGCTAACGTTTCGACTTCGTCAGCA	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
................................................................................................................................................................................AAAAAAAAAACCAAAACAAC...................................................................	20	2	0.50	1	0	1	0	0	0	0
.......................................................................................................................................................................AAAAAAACAAAAAAAAAAACC...........................................................................	21	6	0.33	2	0	2	0	0	0	0
.................................................................................................................................................................ACCAACAAAAAAACAAAAAAA.................................................................................	21	3	0.33	1	0	1	0	0	0	0
........................................................................................................................................................................AAAAAACAAAAAAAAAAACC...........................................................................	20	12	0.25	3	0	3	0	0	0	0
..........................................................................................................................................................................AAAACAAAAAAAAAAACCAAAA.......................................................................	22	7	0.14	1	0	1	0	0	0	0
..........................................................................................................................................................................AAAACAAAAAAAAAAACCAAA........................................................................	21	8	0.13	1	0	1	0	0	0	0
................................................................................................................................................................................AAAAAAAAAACCAAAACA.....................................................................	18	17	0.12	2	0	1	1	0	0	0
..........................................................................................................................................................................AAAACAAAAAAAAAAACC...........................................................................	18	20	0.10	2	0	2	0	0	0	0
.........................................................................................................................................................................AAAAACAAAAAAAAAAACCA..........................................................................	20	13	0.08	1	0	1	0	0	0	0
..........................................................................................................................................................................AAAACAAAAAAAAAAACCA..........................................................................	19	13	0.08	1	0	1	0	0	0	0

droBip1

CCGAGGATGTC--------------------------------ATCGAAATATGCATTCTTGTTATTCTTATAGTCGTGCAGGTA--ACCCACTCAATTATATCCAATCGAAAGAAAAACATTATTCGTTTG---------------GTATCCGCAGCGATACCAACAAA--------------AACCA--ACAACGCCCACC-CACACACAGATCCTAGTCAGCATCGCTGGTTGGTGGCTAACGTATCGACTTCATCGCCA	Size	Hit Count	Total Norm	Total
No Reads

dp5

ATGGCAGGGTGGACAACATAATCATGAAACTTTAGATGAAGGCATTGGCGATGCTATGCTTTTGGTTATCAATATTGTGCAGGTGCGATTCAATTAATCAT-TCACATCA----------ATCATTTGTATGAAAATACGATACGATGCATCTACATAGGT------------------------------------------AGCCATTCAGGTTTCTGTCAATATTGCCGGCTGGTGGCTGGCCGTTCGACTGCGGAGGCA

Size

Hit Count

Total Norm

Total

M022
Male-body

M040
Female-body

M059
Embryo

M062
Head

V043
Embryo

V051
Head

V112
Male-body

SRR902010
Ovary

SRR902011
Testis

SRR902012
CNS imaginal disc

No Reads

droPer2

ATGGCAGGGTGGACAACATAATCATGAAACTTTAGATGAAGGCATTGGCGATGCTATGCTTTTGGTTATCAATATTGTGCAGGTGCGATTCAATTAATCAT-TCACATCA----------ATCATTTGTATGAAAATACGATACGATGCATCTACATAGGT------------------------------------------AGCCATTCAGGTTTCTGTCAATATTGCCGGCTGGTGGCTGGCCGTTCGACTGCGGAGGCA	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
No Reads

droWil2

C------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGGTGGTGGTCAACATTGCTGGCTGGTGGCTAGCTATGCGTTTGCGTAAATA	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
No Reads

dan_2173

scaffold_13334:809629-809742 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-16.4	-15.9	-15.9