ID:

dan_207

Coordinate:

scaffold_13417:2501536-2501593 -

Confidence:

candidate

Class:

Canonical miRNA

Genomic Locale:

antisense_to_intron

[View on UCSC Genome Browser {Cornell Mirror}]

Legend:

mature

star

mismatch in alignment

mismatch in read

Predicted structure

-38.0	-38.0	-38.0

Flybase annnotation

Antisense to intron [Dana\GF20486-in]

No Repeatable elements found

Sense Strand Reads

CTGCCTCACCCGATTCGCCCGGTGCGTTGACCTACAATGCCTAGTCCGGCTAATTGTCAGCTGACCCTTCGGCGAGCTGACTGCCGCAGGGGATGGCCGGCAAGTGCTTGGACTTGGGAAACGGACCCGACACCGCACCGGACAACAACATATCGCGG *********************************....(((((((((((...(((((.(((..((((((((((.......))))).)))))..))).)))))..)).))))).))))...***********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V105 male body	M044 female body	V106 head	V055 head	M058 embryo
..................................................TAATTGTCAGCTGACCCTTCG.......................................................................................	21	0	1	17.00	17	12	3	2	0	0
..................................................TAATTGTCAGCTGACCCTTCGG......................................................................................	22	0	1	7.00	7	0	5	1	1	0
...................................................AATTGTCAGCTGACCCTTCGGTT....................................................................................	23	2	1	5.00	5	2	1	2	0	0
...................................................AATTGTCAGCTGACCCTTCGG......................................................................................	21	0	1	3.00	3	1	1	1	0	0
...................................................AATTGTCAGCTGACCCTTCGGC.....................................................................................	22	0	1	3.00	3	1	1	0	1	0
..................................................TAATTGTCAGCTGACCCTTCGT......................................................................................	22	1	1	2.00	2	1	0	1	0	0
..................................................TAATTGTCAGCTGACCCTTCGGTT....................................................................................	24	2	1	2.00	2	1	0	1	0	0
...................................................AATTGTCAGCTGACCCTTCG.......................................................................................	20	0	1	1.00	1	1	0	0	0	0
..................................................TAATTGTCAGCTGACCCTTCGGCTTT..................................................................................	26	3	1	1.00	1	0	0	1	0	0
..................................................TAATTGTCAGCTGACCCTTCT.......................................................................................	21	1	1	1.00	1	1	0	0	0	0
..................................................TAATTGTCAGCTGACCCTTCA.......................................................................................	21	1	1	1.00	1	1	0	0	0	0
.....................................................................................GCAGGGGATGGCCGGCAAGTGT...................................................	22	1	1	1.00	1	1	0	0	0	0
...................................................AATTGTCAGCTGACCCTTCGGT.....................................................................................	22	1	1	1.00	1	0	1	0	0	0
..................................................TAATTGTCAGTTGACCCTTCG.......................................................................................	21	1	1	1.00	1	1	0	0	0	0
.....................................................................................GCAGGGGATGGCCGGCAAGTG....................................................	21	0	1	1.00	1	1	0	0	0	0
...................................................AATTGTCAGCTGACCCTTCGGCT....................................................................................	23	1	1	1.00	1	0	0	1	0	0
.............................................................................TGACTGACGCGGGGGATGCCC............................................................	21	3	3	0.33	1	0	0	0	1	0
.....................................................................................................................GAAAAGAACCCGACACCACA.....................	20	3	11	0.09	1	0	0	0	0	1
................................................................................................................................AAGACCACACCGGACAACA...........	19	3	20	0.05	1	0	0	0	0	1

Anti-sense strand reads

GACGGAGTGGGCTAAGCGGGCCACGCAACTGGATGTTACGGATCAGGCCGATTAACAGTCGACTGGGAAGCCGCTCGACTGACGGCGTCCCCTACCGGCCGTTCACGAACCTGAACCCTTTGCCTGGGCTGTGGCGTGGCCTGTTGTTGTATAGCGCC ***********************************....(((((((((((...(((((.(((..((((((((((.......))))).)))))..))).)))))..)).))))).))))...*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V055 head
............................................................AACTGCGAAGCCGCTCGA................................................................................	18	2	2	0.50	1	1
..........GATAAGTGGGTCACGCAAC.................................................................................................................................	19	3	9	0.11	1	1

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Type	Alignment
droAna3	scaffold_13417:2501486-2501643 -	dan_207	candidate	CTGCCTCACCCGATTCGCCCGGTGCGTTGACCTACAATGCCTAGTCCGGCTAATTGTCAGCTGACCCTTCGGCGAGCTGACTGCCGCAGGGGATGGCCGGCAAGTGCTTGGACTTGGGAAACGGACCCGACACCGCACCGGACAACAACATATCGCGG
droBip1	scf7180000395874:75808-75947 -			CTGGCTCACCAGGTTCACCCGGTGCGTTGACCTACAATGCTAAGTCCGGCTAATTGTCAGCTGACCCTTCGCCGAGCTGACTGCCGCAGGTGATTGCCGAAAAGTGCTTGGACTTGGGA------------------CCGGACAGCAACACACAGCGG
droKik1	scf7180000302677:223154-223208 +			T--------------------------------------------------------------------CGGCGGATGCTCTCACCCGGGGCATGGCCGGATTGGGATTGGGCTTGGGAAACG-----------------------------------
droEle1	scf7180000490967:672227-672269 +			A--------------------------------------------------------------------------GCTGACTGCCACAAAGGCGGGCCAACAATGGTGGGGATTTGG-----------------------------------------
droRho1	scf7180000779234:293650-293724 +			CT------------TCAGCGGATGCCTTGAC------------------------------------------------------CAGGGGAATGGCCGGATTGGGCTTGGGTTTGGGCAACGAGG------------TGGCCGACATTGGAC-----
droBia1	scf7180000299728:248-278 +			G-----------------------------------------------------------CTGCCTCTCCGCCGGGCTGGCTGCCGCAGG--------------------------------------------------------------------
droTak1	scf7180000415878:263138-263184 -			G--------------------------------------------------------------------------------------------------------------ACTTGGAAAACGGAACCCACACCTGAACGGAAAACACCGGAT-GAGG
droWil2	scf2_1100000004958:376322-376367 +			A---------------------------------------------------AGTATCAAGGGACCCGTGAACAAGTTGACTGCTGCAGAGGGTGAC-------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droAna3

CTGCCTCACCCGATTCGCCCGGTGCGTTGACCTACAATGCCTAGTCCGGCTAATTGTCAGCTGACCCTTCGGCGAGCTGACTGCCGCAGGGGATGGCCGGCAAGTGCTTGGACTTGGGAAACGGACCCGACACCGCACCGGACAACAACATATCGCGG	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
..................................................TAATTGTCAGCTGACCCTTCG.......................................................................................	21	1	17.00	17	3	0	0	0	12	2
..................................................TAATTGTCAGCTGACCCTTCGG......................................................................................	22	1	7.00	7	5	0	0	1	0	1
...................................................AATTGTCAGCTGACCCTTCGGTT....................................................................................	23	1	5.00	5	1	0	0	0	2	2
...................................................AATTGTCAGCTGACCCTTCGG......................................................................................	21	1	3.00	3	1	0	0	0	1	1
...................................................AATTGTCAGCTGACCCTTCGGC.....................................................................................	22	1	3.00	3	1	0	0	1	1	0
..................................................TAATTGTCAGCTGACCCTTCGT......................................................................................	22	1	2.00	2	0	0	0	0	1	1
..................................................TAATTGTCAGCTGACCCTTCGGTT....................................................................................	24	1	2.00	2	0	0	0	0	1	1
...................................................AATTGTCAGCTGACCCTTCG.......................................................................................	20	1	1.00	1	0	0	0	0	1	0
..................................................TAATTGTCAGCTGACCCTTCGGCTTT..................................................................................	26	1	1.00	1	0	0	0	0	0	1
..................................................TAATTGTCAGCTGACCCTTCT.......................................................................................	21	1	1.00	1	0	0	0	0	1	0
..................................................TAATTGTCAGCTGACCCTTCA.......................................................................................	21	1	1.00	1	0	0	0	0	1	0
.....................................................................................GCAGGGGATGGCCGGCAAGTGT...................................................	22	1	1.00	1	0	0	0	0	1	0
...................................................AATTGTCAGCTGACCCTTCGGT.....................................................................................	22	1	1.00	1	1	0	0	0	0	0
.....................................................................................GCAGGGGATGGCCGGCAAGTG....................................................	21	1	1.00	1	0	0	0	0	1	0
...................................................AATTGTCAGCTGACCCTTCGGCT....................................................................................	23	1	1.00	1	0	0	0	0	0	1

droBip1

CTGGCTCACCAGGTTCACCCGGTGCGTTGACCTACAATGCTAAGTCCGGCTAATTGTCAGCTGACCCTTCGCCGAGCTGACTGCCGCAGGTGATTGCCGAAAAGTGCTTGGACTTGGGA------------------CCGGACAGCAACACACAGCGG	Size	Hit Count	Total Norm	Total
No Reads

droKik1

T--------------------------------------------------------------------CGGCGGATGCTCTCACCCGGGGCATGGCCGGATTGGGATTGGGCTTGGGAAACG-----------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEle1

A--------------------------------------------------------------------------GCTGACTGCCACAAAGGCGGGCCAACAATGGTGGGGATTTGG-----------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droRho1

CT------------TCAGCGGATGCCTTGAC------------------------------------------------------CAGGGGAATGGCCGGATTGGGCTTGGGTTTGGGCAACGAGG------------TGGCCGACATTGGAC-----	Size	Hit Count	Total Norm	Total
No Reads

droBia1

G-----------------------------------------------------------CTGCCTCTCCGCCGGGCTGGCTGCCGCAGG--------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droTak1

G--------------------------------------------------------------------------------------------------------------ACTTGGAAAACGGAACCCACACCTGAACGGAAAACACCGGAT-GAGG	Size	Hit Count	Total Norm	Total
No Reads

droWil2

A---------------------------------------------------AGTATCAAGGGACCCGTGAACAAGTTGACTGCTGCAGAGGGTGAC-------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
.......................................................................ACAAGTTGACTGCTGCAGAGGGTGA..............................................................	25	2	0.50	1	0	0	0	1	0
................................................................CCCGTGAACAAGTTGACTGCTG........................................................................	22	20	0.20	4	0	4	0	0	0
................................................................CCCGTGAACAAGTTGACTGCTGC.......................................................................	23	20	0.20	4	0	4	0	0	0
.................................................................CCGTGAACAAGTTGACTGCTGC.......................................................................	22	20	0.10	2	0	2	0	0	0
................................................................CCCGTGAACAAGTTGACTGCTGCAG.....................................................................	25	20	0.05	1	0	0	0	1	0
................................................................CCCGTGAACAAGTTGACTG...........................................................................	19	20	0.05	1	1	0	0	0	0
....................................................................TGAACAAGTTGACTGCTGCAG.....................................................................	21	20	0.05	1	0	0	0	1	0
.................................................................CCGTGAACAAGTTGACTGCTGCAA.....................................................................	24	20	0.05	1	0	1	0	0	0
.................................................................CCGTGAACAAGTTGACTGCTGCA......................................................................	23	20	0.05	1	0	0	0	1	0

Generated: 10/20/2015 at 06:55 PM