ID:

dan_205

 Coordinate:

scaffold_12943:2737363-2737407 +

 Confidence:

candidate

 Class:

Canonical miRNA

 Genomic Locale:

intergenic

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-23.8 -23.6 -23.3

Flybase annnotation

intergenic

No Repeatable elements found

Sense Strand Reads

GGTAAGGAGTTCTTCGCTTTAGAGTCGGCTCATGGCTCTAAAGAAACTCTCGCTGATCTGGGGGAGCGTCTGCGAGCGGCCCCAGGTGAGCTTCCAATGTCAATGCCTATGATGCGAATAGACTGGCCAACGGAGTTCCATGATG

***********************************................(((.((((((((..((........))..)))))))).)))...................*********************************** 		Read size # Mismatch Hit Count Total Norm Total V105

male body		 V055

head

 V106

head

 M044

female body

 M058

embryo
.........................................................................GAGCGGCCCCAGGTGAGCTTC................................................... 21 0 1 627.00 627 536 34 29 28 0
.........................................................................GAGCGGCCCCAGGTGAGCTT.................................................... 20 0 1 38.00 38 36 0 1 1 0
.........................................................................GAGCGGCCCCAGGTGAGCTTCT.................................................. 22 1 1 24.00 24 23 0 1 0 0
.........................................................................GATCGGCCCCAGGTGAGCTTC................................................... 21 1 1 9.00 9 5 0 3 1 0
.........................................................................GAGCGGCCCCAGGTGAGCT..................................................... 19 0 1 8.00 8 5 0 3 0 0
.........................................................................GAGCGGCCCCAGGTGAGCTTG................................................... 21 1 1 6.00 6 5 0 0 1 0
..........................................................................AGCGGCCCCAGGTGAGCTTC................................................... 20 0 1 5.00 5 2 0 0 3 0
.........................................................................GAGCGGCCCCAGGTGAGCTTT................................................... 21 1 1 5.00 5 3 1 0 1 0
........................................................................CGAGCGGCCCCAGGTGAGCTTC................................................... 22 0 1 5.00 5 3 1 0 1 0
.........................................................................GAGCGGCCCCAGGTGAGCTTA................................................... 21 1 1 4.00 4 4 0 0 0 0
.........................................................................GAGCGGCCCCAGGCGAGCTTC................................................... 21 1 1 4.00 4 1 3 0 0 0
.........................................................................GAGAGGCCCCAGGTGAGCTTC................................................... 21 1 1 4.00 4 3 0 1 0 0
.........................................................................GAGCGGCCCCAGCTGAGCTTC................................................... 21 1 1 3.00 3 3 0 0 0 0
.........................................................................GAGCGGCCCCATGTGAGCTTC................................................... 21 1 1 3.00 3 2 0 1 0 0
........................................................................CGAGCGGCCCCAGGTGAGC...................................................... 19 0 1 3.00 3 1 1 1 0 0
.........................................................................GTGCGGCCCCAGGTGAGCTTC................................................... 21 1 1 3.00 3 2 1 0 0 0
.........................................................................GAGCGGCCCCAGGTGCGCTTC................................................... 21 1 1 3.00 3 2 1 0 0 0
.........................................................................GGGCGGCCCCAGGTGAGCTTC................................................... 21 1 1 3.00 3 3 0 0 0 0
..............................................................................................CAATGTCAATGCCTATGATGCG............................. 22 0 1 3.00 3 1 1 1 0 0
.........................................................................GAGCGGCCCTAGGTGAGCTTC................................................... 21 1 1 3.00 3 2 0 1 0 0
.........................................................................GAGCAGCCCCAGGTGAGCTTC................................................... 21 1 1 3.00 3 1 2 0 0 0
.........................................................................GAGCGGCCTCAGGTGAGCTTC................................................... 21 1 1 2.00 2 2 0 0 0 0
........................................................................CGAGCGGCCCCAGGTGAGCTT.................................................... 21 0 1 2.00 2 2 0 0 0 0
.........................................................................GAGCGGCCGCAGGTGAGCTTC................................................... 21 1 1 2.00 2 1 0 0 1 0
.........................................................................GAGCGGCCCCAAGTGAGCTTC................................................... 21 1 1 2.00 2 2 0 0 0 0
.........................................................................GAGCGGCCACAGGTGAGCTTC................................................... 21 1 1 2.00 2 2 0 0 0 0
.........................................................................GAGCGGCCCCAGTTGAGCTTC................................................... 21 1 1 2.00 2 2 0 0 0 0
.........................................................................GAGCGGCCCCAGGAGAGCTTC................................................... 21 1 1 2.00 2 1 1 0 0 0
.........................................................................GAGCTGCCCCAGGTGAGCTTC................................................... 21 1 1 2.00 2 2 0 0 0 0
.........................................................................GCGCGGCCCCAGGTGAGCTTC................................................... 21 1 1 2.00 2 2 0 0 0 0
.........................................................................GAGCGGCCCCAGGGGAGCTTC................................................... 21 1 1 2.00 2 1 1 0 0 0
........................................................................CGAGCGGCCCCAGGTGAGT...................................................... 19 1 1 2.00 2 0 0 2 0 0
.........................................................................GAGTGGCCCCAGGTGAGCTTC................................................... 21 1 1 2.00 2 2 0 0 0 0
.........................................................................GAGCGTCCCCAGGTGAGCTTCT.................................................. 22 2 1 1.00 1 1 0 0 0 0
......................................TAAAGAAACTCTCGCTGATCTGGGGG................................................................................. 26 0 1 1.00 1 1 0 0 0 0
.........................................................................GAGCGGCCACAGGTGCGCTTC................................................... 21 2 1 1.00 1 0 0 1 0 0
.........................................................................GAGCGGCCCCAGGTGAGCGTC................................................... 21 1 1 1.00 1 0 1 0 0 0
.........................................................................GAGCGGCCCCACGTGAGCTTC................................................... 21 1 1 1.00 1 0 1 0 0 0
..............................................................................................CAATGTCAATGCCTATATTGCG............................. 22 2 1 1.00 1 0 1 0 0 0
.........................................................................GAGCGGCCCCAGGTGAGATTC................................................... 21 1 1 1.00 1 1 0 0 0 0
.........................................................................GCTCGGCCCCAGGTGAGCTTC................................................... 21 2 1 1.00 1 1 0 0 0 0
..........TCTTCGCTTTAGAGTCGGCTCATGG.............................................................................................................. 25 0 1 1.00 1 1 0 0 0 0
.........................................................................AAGCGGCCCCAGGTGAGCTTC................................................... 21 1 1 1.00 1 0 1 0 0 0
.........................................................................GAGCGGCCCCAGGTGTGCTTC................................................... 21 1 1 1.00 1 1 0 0 0 0
.........................................................................GAGCGGCCCCAGGTTAGCTTC................................................... 21 1 1 1.00 1 1 0 0 0 0
.........................................................................GAGCGGCCCCGGGTCAGCTTC................................................... 21 2 1 1.00 1 1 0 0 0 0
.........................................................................GAGCGGCCCCAGGTGAGC...................................................... 18 0 1 1.00 1 1 0 0 0 0
..............................CATGGCTCTAAAGAAACTCTC.............................................................................................. 21 0 1 1.00 1 0 0 0 0 1
....................................................CTGATCTGGGGGAGCGTCT.......................................................................... 19 0 1 1.00 1 0 0 0 1 0
.........................................................................GATCGTCCCCAGGTGAGCTTC................................................... 21 2 1 1.00 1 1 0 0 0 0
........................................................................CGAGCGGCCCCAGGTGAGCTTT................................................... 22 1 1 1.00 1 1 0 0 0 0
..............................................................................................CAATGTCAATGCCTATGATGCT............................. 22 1 1 1.00 1 1 0 0 0 0
..............................................................................................CAATGTCAATGCCTATGATTCG............................. 22 1 1 1.00 1 0 0 1 0 0
.........................................................................GAGCGGCCCCCGGTGAGCCTC................................................... 21 2 1 1.00 1 0 1 0 0 0
.........................................................................GAGCGGCCCCAGGTGAGCTTCTG................................................. 23 2 1 1.00 1 0 1 0 0 0
..........................................................................AGCGGCCCCAGGTGAGCTT.................................................... 19 0 1 1.00 1 0 0 0 1 0
.........................................................................GAGCGGACCCAGGTGAGCTTC................................................... 21 1 1 1.00 1 1 0 0 0 0
..........................................................................AGCGGCCCCAGGAGAGCTTC................................................... 20 1 1 1.00 1 1 0 0 0 0
.........................................................................GAGCGGCCCCAGGTGAGCTAC................................................... 21 1 1 1.00 1 0 1 0 0 0
.........................................................................AAGCGGCCCCAGGTGAGCTCC................................................... 21 2 1 1.00 1 0 1 0 0 0
........................................................................CGAGCGGCCCCAGGTGAGCT..................................................... 20 0 1 1.00 1 1 0 0 0 0
.........................................................................GAGCGGCCCCAGGTGAGCC..................................................... 19 1 1 1.00 1 1 0 0 0 0
.........................................................................GAGCGGCCCCAGGTGAGCTTCA.................................................. 22 1 1 1.00 1 1 0 0 0 0
.........................................................................GAGCGGCCCCAGGTGAGCCTC................................................... 21 1 1 1.00 1 0 1 0 0 0
........................................................................CGAGCGGCCCCAGGTGATT...................................................... 19 2 1 1.00 1 1 0 0 0 0
.........................................................................GAGCGGCACCAGGTGAGCTTC................................................... 21 1 1 1.00 1 1 0 0 0 0
.........................................................................GAGCGGCCCCAGGTGTGCTTCT.................................................. 22 2 1 1.00 1 1 0 0 0 0
........................................................................CGAGCGGCCCCAGGTGAGTT..................................................... 20 1 1 1.00 1 1 0 0 0 0
..............................................................................................CAATGTCAATGCCTATGATGCGTTTT......................... 26 3 1 1.00 1 1 0 0 0 0
........................................................................CGAGCGGCCCCAGGTGAGCTTCT.................................................. 23 1 1 1.00 1 1 0 0 0 0
..............................................................................................CAATGTCAATGCCTATGATGCGTT........................... 24 2 1 1.00 1 1 0 0 0 0
.........................................................................GATCGGCCCCAGGTGAGCTCC................................................... 21 2 1 1.00 1 1 0 0 0 0
.........................................................................GAGCGTCCCCAGGTGAGCTTC................................................... 21 1 1 1.00 1 0 0 1 0 0
.........................................................................GAGCGGCCCCATGTGAGCTT.................................................... 20 1 1 1.00 1 1 0 0 0 0
............................................................................CGGCCCCAGGTGAGCTTC................................................... 18 0 1 1.00 1 1 0 0 0 0
.........................................................................GAGCGGCCCCAGTTGAGCT..................................................... 19 1 1 1.00 1 1 0 0 0 0
.........................................................................GAGCGGCCCCAGATGAGCTTC................................................... 21 1 1 1.00 1 1 0 0 0 0
.........................................................................GAGCGGCCCCAGGTGAGTT..................................................... 19 1 1 1.00 1 0 0 0 1 0
.........................................................................GAGCGGCCCCAGGTGAACTT.................................................... 20 1 1 1.00 1 1 0 0 0 0
.................................................................................CCAGGTGAGCTACCCATGTC............................................ 20 2 1 1.00 1 0 0 0 1 0
.........................................................................GGGCGGCCCCAGGTAAGCTTC................................................... 21 2 1 1.00 1 1 0 0 0 0
.........................................................................GAGCGTCCCCAGGTGATCTTC................................................... 21 2 1 1.00 1 1 0 0 0 0
.........................................................................GAGCGGCCCCAGGCCAGCTTC................................................... 21 2 1 1.00 1 0 1 0 0 0
.........................................................................GAGCGGCCACTGGTGAGCTTC................................................... 21 2 2 0.50 1 1 0 0 0 0
.........................................................................GAGCCGCCCCAGGTGAGCTTC................................................... 21 1 2 0.50 1 1 0 0 0 0
.........................................................................GAGCGGCCCCTGGTGAGCTTC................................................... 21 1 2 0.50 1 1 0 0 0 0
.........................................................................GACAGGCCCCAGGTGAGCTTC................................................... 21 2 2 0.50 1 1 0 0 0 0
...........................................................................GAGCCCCCAGGTGAGCTTC................................................... 19 2 3 0.33 1 1 0 0 0 0
.................................................................................CCAGGTGACCTACCCATGTC............................................ 20 3 3 0.33 1 0 0 0 1 0
.........................................................................GAGCGGCCCCAGGCCATCTTC................................................... 21 3 4 0.25 1 0 1 0 0 0
.................................................................................CCAGGTGAGCTATCCATGTC............................................ 20 3 5 0.20 1 0 0 0 1 0
..................................................................................CAGGTGACCTACCCATGTC............................................ 19 3 7 0.14 1 0 0 0 1 0
...................TAGAGTCGGTTCAGGGC............................................................................................................. 17 2 8 0.13 1 0 1 0 0 0
.........................................................................GAGCGGCCCCAGAAGTGCTTC................................................... 21 3 11 0.09 1 0 1 0 0 0
..................................................................................CAGGTGACCTACCCATGT............................................. 18 3 20 0.05 1 0 0 0 1 0

Anti-sense strand reads

CCATTCCTCAAGAAGCGAAATCTCAGCCGAGTACCGAGATTTCTTTGAGAGCGACTAGACCCCCTCGCAGACGCTCGCCGGGGTCCACTCGAAGGTTACAGTTACGGATACTACGCTTATCTGACCGGTTGCCTCAAGGTACTAC

***********************************................(((.((((((((..((........))..)))))))).)))...................*********************************** 		Read size # Mismatch Hit Count Total Norm Total M058

embryo

 M044

female body
.................................................................CGCAGACGCTCGCCGGGGT............................................................. 19 0 1 1.00 1 1 0
........................................................................................................................CTGCCCGGTTGCGTCCAGGT..... 20 3 3 0.33 1 0 1

Re-alignment of all predicted orthologs

Species Coordinate ID Type Alignment
droAna3 scaffold_12943:2737313-2737457 + dan_205 candidate GGTAAGGAGTTCTTCGCTTTAGAGTCGGCTCATGGCTCTAAAGAAACT------CTCGCTGATCTGGGGGAGCGTCTGCGAGCGGCCCCAGGTGAGCTTCCAATGTCAATGCCTATGATGCGAATAGACTGGCCAACGGAGTTCCATGATG
droBip1 scf7180000396535:2134218-2134360 + GGTAAGGACTTTTTCGCTTCAGTGTCTGCTTATGGCGGTGAAGGAATTCCCATTCCCGCTTACTAGGGCTATTGTCTGCAAACGGCGCCAGCGGAGCTGCCAATATCAATGCCAAAGATGCGAATAGGGGAGTCATCTG--------GAAG

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droAna3
GGTAAGGAGTTCTTCGCTTTAGAGTCGGCTCATGGCTCTAAAGAAACT------CTCGCTGATCTGGGGGAGCGTCTGCGAGCGGCCCCAGGTGAGCTTCCAATGTCAATGCCTATGATGCGAATAGACTGGCCAACGGAGTTCCATGATGSizeHit CountTotal NormTotalM044

Female-body		M058

Embryo		V039

Embryo		V055

Head		V105

Male-body		V106

Head
...............................................................................GAGCGGCCCCAGGTGAGCTTC...................................................211627.0062728003453629
...............................................................................GAGCGGCCCCAGGTGAGCTT....................................................20138.00381000361
...............................................................................GAGCGGCCCCAGGTGAGCTTCT..................................................22124.00240000231
...............................................................................GAGCGGCCCCAGGTGAGCT.....................................................1918.008000053
...............................................................................GAGCGGCCCCAGGTGAGCTTG...................................................2116.006100050
................................................................................AGCGGCCCCAGGTGAGCTTC...................................................2015.005300020
...............................................................................GAGCGGCCCCAGGTGAGCTTT...................................................2115.005100130
..............................................................................CGAGCGGCCCCAGGTGAGCTTC...................................................2215.005100130
...............................................................................GAGCGGCCCCAGGTGAGCTTA...................................................2114.004000040
..............................................................................CGAGCGGCCCCAGGTGAGC......................................................1913.003000111
....................................................................................................CAATGTCAATGCCTATGATGCG.............................2213.003000111
..............................................................................CGAGCGGCCCCAGGTGAGCTT....................................................2112.002000020
..............................................................................CGAGCGGCCCCAGGTGAGT......................................................1912.002000002
......................................TAAAGAAACT------CTCGCTGATCTGGGGG.................................................................................2611.001000010
..........TCTTCGCTTTAGAGTCGGCTCATGG....................................................................................................................2511.001000010
...............................................................................GAGCGGCCCCAGGTGAGC......................................................1811.001000010
..............................CATGGCTCTAAAGAAACT------CTC..............................................................................................2111.001010000
..........................................................CTGATCTGGGGGAGCGTCT..........................................................................1911.001100000
..............................................................................CGAGCGGCCCCAGGTGAGCTTT...................................................2211.001000010
....................................................................................................CAATGTCAATGCCTATGATGCT.............................2211.001000010
...............................................................................GAGCGGCCCCAGGTGAGCTTCTG.................................................2311.001000100
................................................................................AGCGGCCCCAGGTGAGCTT....................................................1911.001100000
..............................................................................CGAGCGGCCCCAGGTGAGCT.....................................................2011.001000010
...............................................................................GAGCGGCCCCAGGTGAGCC.....................................................1911.001000010
...............................................................................GAGCGGCCCCAGGTGAGCTTCA..................................................2211.001000010
..............................................................................CGAGCGGCCCCAGGTGATT......................................................1911.001000010
..............................................................................CGAGCGGCCCCAGGTGAGCTTCT..................................................2311.001000010
....................................................................................................CAATGTCAATGCCTATGATGCGTT...........................2411.001000010
..................................................................................CGGCCCCAGGTGAGCTTC...................................................1811.001000010
droBip1
GGTAAGGACTTTTTCGCTTCAGTGTCTGCTTATGGCGGTGAAGGAATTCCCATTCCCGCTTACTAGGGCTATTGTCTGCAAACGGCGCCAGCGGAGCTGCCAATATCAATGCCAAAGATGCGAATAGGGGAGTCATCTG--------GAAGSizeHit CountTotal NormTotal
No Reads

Generated: 10/20/2015 at 07:33 PM