dan_1625

exon [dana_GLEANR_1009:2]; CDS [Dana\GF10133-cds]; intron [Dana\GF10133-in]; Antisense to intron [Dana\GF12903-in]

Sense Strand Reads

##################################################--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- GAAGGAACTCCCACCTTCTAGTGGGGAATAGGCTGAAGGACCCCCCGTGGGTAAGTTCGACAGTCTCAAGTGCGGGATTTAATGCGAGTGCGCAAGGATCTGCGATACTGATCGCAGCTACTGAGCTTAAGGACCCCCTGTGGGTAAGTCCGGCGGCGGTACGCGAATCAGCAAAAGAGTATAAATCAAGGAGCAAGTGGGAAAAGGATCAAGGATCCGCGGTGGAAATGAAGCCCATGGGTAAAACGAGT *****************************************************.....((((.((....((((.((((..((((....)))).)))))))))).)))).******************************************************************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M044 female body	M058 embryo	V106 head	V039 embryo	V105 male body	V055 head
....................................................................................................................................................................................................................GGTTCCGGGGTCGAAATGA....................	19	3	18	2.11	38	29	0	6	0	2	1
...................................................................................................................................................................................................................AGGTTCCGGGGTCGAAATGA....................	20	3	4	1.75	7	6	0	1	0	0	0
...................................................................................................................................................................................................................AGGTTCCGGGGTCGAAATG.....................	19	3	12	1.00	12	8	0	0	0	3	1
...................................................................................................................................................................................................................AGGTTCCGCGGTCGAAATGAC...................	21	3	2	0.50	1	1	0	0	0	0	0
..................................................................................................................................................................................................................AAGGATCCGCGGTGGAAATGAAGCCC...............	26	0	2	0.50	1	0	1	0	0	0	0
.......................................................................................GTGCGCAAGGATCTGCGATACTGA............................................................................................................................................	24	0	2	0.50	1	1	0	0	0	0	0
.............................................................................................AAGGATCTGCGATACTTATCGCAGC.....................................................................................................................................	25	1	2	0.50	1	0	0	0	1	0	0
.....................................................................................................................................................................................TAAATCAAGGAGCAAGTGGGA.................................................	21	0	4	0.25	1	1	0	0	0	0	0
.......................................................................................................................ACTGAGATGTAGGACCCCCTGT..............................................................................................................	22	3	4	0.25	1	0	0	0	0	0	1
....................GTGGGGTATGGGCTGAAG.....................................................................................................................................................................................................................	18	2	10	0.20	2	2	0	0	0	0	0
.......................................................TTCGACAGTCTCAAGTGCGGGA..............................................................................................................................................................................	22	0	5	0.20	1	0	1	0	0	0	0
..........................................................................................................................................................................................................AAAGGATCAAGGATCCGCGGTG...........................	22	0	13	0.15	2	2	0	0	0	0	0
..................................................................................................................................................................................................................GAGGTTCCGCGGTCGAAAT......................	19	3	9	0.11	1	0	0	0	0	1	0
.........................................................................................................................................................................................................AAAAGGATCAAGGATCCGCGGTGG..........................	24	0	13	0.08	1	1	0	0	0	0	0
..........................................................................................................................................................................................................AAAGGATCAAGGATCCGCGGTGGAA........................	25	0	13	0.08	1	0	1	0	0	0	0
............................................................................................................................................................................................................AGGATCAAGGATCCGCGGTGGA.........................	22	0	15	0.07	1	1	0	0	0	0	0
.........................................................................................................................................................................................................AAAAGGATCAAGGATCCGCGGT............................	22	0	15	0.07	1	0	0	0	0	1	0
......................................................................................................................................................................................................................ATCAGGGGTGGAAATGA....................	17	2	20	0.05	1	0	0	0	0	0	1
.................................................................................................................................................................................................CAAGTGGGAAAAGGATCAAGGATCCGC...............................	27	0	20	0.05	1	1	0	0	0	0	0
............................TAGGCTGAAGGACCCCCCGTGGGTAAG....................................................................................................................................................................................................	27	0	20	0.05	1	0	0	0	1	0	0
...................................................................................................................................................................................................AGTGGGAAAAGGATCAAGGATCC.................................	23	0	20	0.05	1	1	0	0	0	0	0
.................................................................................................................................................AAGTCCGGCGGCGGTACGCGAA....................................................................................	22	0	20	0.05	1	1	0	0	0	0	0
................................................................................................................................................TAAGTCCGTCGGCGGTACGCGAATCAGC...............................................................................	28	1	20	0.05	1	1	0	0	0	0	0
..............................................................................................................................TTAAGGACCCCCTGTGGGTAAGTC.....................................................................................................	24	0	20	0.05	1	0	1	0	0	0	0

##################################################---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
GAAGGAACTCCCACCTTCTAGTGGGGAATAGGCTGAAGGACCCCCCGTGGGTAAGTTCGACAGTCTCAAGTGCGGGATTTAATGCGAGTGCGCAAGGATCTGCGATACTGATCGCAGCTACTGAGCTTAAGGACCCCCTGTGGGTAAGTCCGGCGGCGGTACGCGAATCAGCAAAAGAGTATAAATCAAGGAGCAAGTGGGAAAAGGATCAAGGATCCGCGGTGGAAATGAAGCCCATGGGTAAAACGAGT
*******************************************************.....((((.((....((((.((((..((((....)))).)))))))))).)))).********************************************************************************************************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M044
female body

M058
embryo

V106
head

V039
embryo

V105
male body

V055
head

....................................................................................................................................................................................................................GGTTCCGGGGTCGAAATGA....................

2.11

1.75

1.00

0.50

.......................................................................................GTGCGCAAGGATCTGCGATACTGA............................................................................................................................................

0.50

.............................................................................................AAGGATCTGCGATACTTATCGCAGC.....................................................................................................................................

0.50

.....................................................................................................................................................................................TAAATCAAGGAGCAAGTGGGA.................................................

0.25

.......................................................................................................................ACTGAGATGTAGGACCCCCTGT..............................................................................................................

0.25

....................GTGGGGTATGGGCTGAAG.....................................................................................................................................................................................................................

0.20

.......................................................TTCGACAGTCTCAAGTGCGGGA..............................................................................................................................................................................

0.20

0.15

0.11

0.08

0.07

0.05

.................................................................................................................................................................................................CAAGTGGGAAAAGGATCAAGGATCCGC...............................

0.05

............................TAGGCTGAAGGACCCCCCGTGGGTAAG....................................................................................................................................................................................................

0.05

...................................................................................................................................................................................................AGTGGGAAAAGGATCAAGGATCC.................................

0.05

.................................................................................................................................................AAGTCCGGCGGCGGTACGCGAA....................................................................................

0.05

................................................................................................................................................TAAGTCCGTCGGCGGTACGCGAATCAGC...............................................................................

0.05

..............................................................................................................................TTAAGGACCCCCTGTGGGTAAGTC.....................................................................................................

0.05

Anti-sense strand reads

CTTCCTTGAGGGTGGAAGATCACCCCTTATCCGACTTCCTGGGGGGCACCCATTCAAGCTGTCAGAGTTCACGCCCTAAATTACGCTCACGCGTTCCTAGACGCTATGACTAGCGTCGATGACTCGAATTCCTGGGGGACACCCATTCAGGCCGCCGCCATGCGCTTAGTCGTTTTCTCATATTTAGTTCCTCGTTCACCCTTTTCCTAGTTCCTAGGCGCCACCTTTACTTCGGGTACCCATTTTGCTCA ******************************************************************************************************************************************.....((((.((....((((.((((..((((....)))).)))))))))).)))).*****************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M044 female body	M058 embryo	V039 embryo	V055 head	V106 head
............TGGAAGATCACCCCTTATCCGACTTC.....................................................................................................................................................................................................................	26	0	1	1.00	1	0	1	0	0	0
..........................TTATCCGACTTCCTGGGGGGCACCCAT......................................................................................................................................................................................................	27	0	1	1.00	1	1	0	0	0	0
........................................................................................................................................................CGCCGCCATGCGCTTAGTCGTT.............................................................................	22	0	1	1.00	1	1	0	0	0	0
...................................................................................................................................................CAGGCCGCCGCCATGCGCTTAGTCGTTT............................................................................	28	0	1	1.00	1	1	0	0	0	0
..............................................................................................................................................CCATTCAGGCCGCCGCCATGCGCTT....................................................................................	25	0	20	0.70	14	0	14	0	0	0
................................................................................TTACGCTCACGCGTTCCTAGACGCTAT................................................................................................................................................	27	0	2	0.50	1	1	0	0	0	0
..........................................................................................................................................................CCGCCATGCGCTTAGTCGTT.............................................................................	20	0	2	0.50	1	0	1	0	0	0
......................................................................................................................................................GCCGCCGCCATGAGCTTAGTCGTT.............................................................................	24	1	2	0.50	1	0	1	0	0	0
.....................................................TCAAGCTGTCAGAGTTCACGCCCTAAAT..........................................................................................................................................................................	28	0	5	0.40	2	2	0	0	0	0
..........................................................................................................................................................................................GTTCCTCGTTCACCCTTTTCCTAGTT.......................................	26	0	20	0.35	7	7	0	0	0	0
.............................................................................................................................................................................................CCTCGTTCACCCTTTTCCTAGTTCCT....................................	26	0	20	0.30	6	0	6	0	0	0
.............................................................................................................................................CCCATTCAGGCCGCCGCCATGCGC......................................................................................	24	0	20	0.30	6	6	0	0	0	0
..................................................................................................AGACGCTATGACTCCCGTTG.....................................................................................................................................	20	3	4	0.25	1	0	0	0	1	0
..........................................................................................................................CTCGAATTCCTGGGGGACATCC...........................................................................................................	22	1	4	0.25	1	0	1	0	0	0
.......................................CGGGGGGCACCCATTCAAGCTGTCAGAG........................................................................................................................................................................................	28	1	5	0.20	1	0	1	0	0	0
.........................................................................................................................................................................................AGTTCCTCGTTCACCCTTTTCCTAGTT.......................................	27	0	20	0.20	4	3	1	0	0	0
..................................................................................................................................................TCAGGCCGCCGCCATGCGCTTAGTC................................................................................	25	0	20	0.20	4	4	0	0	0	0
.....................................................................................................................................GGGGGACACCCATTCAGGCCGCCGCCA...........................................................................................	27	0	20	0.20	4	4	0	0	0	0
.....................................................................CACGCCCTAAATTACGCTCACGCGT.............................................................................................................................................................	25	0	5	0.20	1	0	1	0	0	0
...........................................GGGCACCCATTCAAGCTGTCAG..........................................................................................................................................................................................	22	0	11	0.18	2	2	0	0	0	0
...........................................GGGCACCCATTCAAGCTGTCATAGT.......................................................................................................................................................................................	25	1	6	0.17	1	0	1	0	0	0
............................................................GTCAGAGTTCACGCCCTAAATTACGCT....................................................................................................................................................................	27	0	6	0.17	1	1	0	0	0	0
....................................................................TCACGCCCTAAATTACGCTCACG................................................................................................................................................................	23	0	6	0.17	1	0	1	0	0	0
.............................................................................................................................................CCCATTCAGGCCGCCGCCATGCG.......................................................................................	23	0	20	0.15	3	3	0	0	0	0
........................................................................................................................................................................................TAGTTCCTCGTTCACCCTTTTCCTAGTT.......................................	28	0	20	0.15	3	3	0	0	0	0
.............................................................................................................................................................................................CCTCGTTCACCCTTTTCCTAGTTC......................................	24	0	20	0.15	3	1	2	0	0	0
.............................................................................................................................................CCCATTCAGGCCGCCGCCATG.........................................................................................	21	0	20	0.15	3	3	0	0	0	0
...................................................................................................................................................CAGGCCGCCGCCATGCGCTTAGT.................................................................................	23	0	20	0.15	3	2	0	1	0	0
...................................................................................................................................................CAGGCCGCCGCCATGCGCTTAGTC................................................................................	24	0	20	0.15	3	2	1	0	0	0
...............................................ACCCATTCAAGCTGTCAGAGTTCAC...................................................................................................................................................................................	25	0	7	0.14	1	1	0	0	0	0
............................................GCCACCCATTCAAGCTGTCAGAGT.......................................................................................................................................................................................	24	1	7	0.14	1	0	0	1	0	0
..........................................................................................................................CTCGAAATCCTGTGGGAC...............................................................................................................	18	2	7	0.14	1	0	0	0	0	1
.......................................................AAGCTGTCAGAGTTCACGC.................................................................................................................................................................................	19	0	8	0.13	1	0	1	0	0	0
.......................................TGGGGGGCACCCATTCAAGCTGTCAG..........................................................................................................................................................................................	26	0	8	0.13	1	1	0	0	0	0
.........................................GGGGGCACCCATTCAAGCTGTCAG..........................................................................................................................................................................................	24	0	9	0.11	1	1	0	0	0	0
..........................................................................................................................................ACACCCATTCAGGCCGCCGCCATGC........................................................................................	25	0	20	0.10	2	2	0	0	0	0
............................................................................................................................................................................................TCCTCGTTCACCCTTTTCCTAGTTC......................................	25	0	20	0.10	2	1	0	1	0	0
.........................................GGGGGCACCCATTCAAGCTGT.............................................................................................................................................................................................	21	0	10	0.10	1	1	0	0	0	0
........................................................................................................................................GGACACCCATTCAGGCCGCCGCCATGCGC......................................................................................	29	0	20	0.10	2	2	0	0	0	0
.......................................................................................................................................................................................................CCTTTTCCTAGTTCCTAGGCGCCAC...........................	25	0	13	0.08	1	1	0	0	0	0
......................................................................................................................................................................................................CCCTTTTCCTAGTTCCTAGGCGCCAC...........................	26	0	13	0.08	1	1	0	0	0	0
.......................................................................................................................................................................................TTAGTTCCTCGTTCACCCTTTTCCTAGT........................................	28	0	20	0.05	1	1	0	0	0	0
..........................................................................................................................................ACACCCATTCAGGCCGCCGCCA...........................................................................................	22	0	20	0.05	1	1	0	0	0	0
...........................................................................................................................................................................................TTCCTCGTTCACCCTTTTCCTAGTT.......................................	25	0	20	0.05	1	0	0	1	0	0
......................................................................................................................................GGGGACACCCATTCAGGCCGCCTC.............................................................................................	24	1	20	0.05	1	0	1	0	0	0
..........................................................CTGTCAGAGTTCACGCCCTAAAT..........................................................................................................................................................................	23	0	20	0.05	1	0	1	0	0	0
...........................................................................................................................................CACCCATTCAGGCCGCCGCCATGCGCTT....................................................................................	28	0	20	0.05	1	0	1	0	0	0
...........................................................................................................................................................................................TTCCTCGTTCACCCTTTTCCTAGT........................................	24	0	20	0.05	1	1	0	0	0	0
...........................................................................................................................................................................................TTCCTCGTTCACCCTTATCCTAGTT.......................................	25	1	20	0.05	1	1	0	0	0	0
..............................................................................................................................................................................................CTCGTTCACCCTTTTCCTAGTTCATA...................................	26	1	20	0.05	1	0	1	0	0	0
.........................................................GCTGTCAGAGTTCACGCCCT..............................................................................................................................................................................	20	0	20	0.05	1	1	0	0	0	0
............................................................................................................................................................................................TCCTCGTTCACCCTTTTCCTAGTT.......................................	24	0	20	0.05	1	1	0	0	0	0
.................................................................................................................................................TTCAGGCCGCCGCCATGCGCTTAGTC................................................................................	26	0	20	0.05	1	1	0	0	0	0
..................................................................................................................................................CCAGGCCGCCGCCATGCGCTTAGT.................................................................................	24	1	20	0.05	1	0	1	0	0	0
.................................................................................................................................................TTCAGGCCGCCGCCATGCGCTTAGGC................................................................................	26	1	20	0.05	1	0	1	0	0	0
..............................................................................................................................................CCATTCAGGCCGCCGCCATGCGC......................................................................................	23	0	20	0.05	1	1	0	0	0	0
..........................................................................................................................................................................................GTTCCTCGTTCACCCTTTTCCTAGTTCC.....................................	28	0	20	0.05	1	1	0	0	0	0
............................................................................................................................................ACCCATTCAGGCCGCCGCCATGCGCTTAG..................................................................................	29	0	20	0.05	1	1	0	0	0	0
.....................................................................................................................................GGGGGACACCCATTCAGGCCGCCGCCATG.........................................................................................	29	0	20	0.05	1	0	1	0	0	0
...........................................................................................................................................................................................TTCCTCGTTCACCCTTTTCCT...........................................	21	0	20	0.05	1	0	1	0	0	0
................................................................................................................................................ATTCAGGCCGCCGCCATGCGCTTA...................................................................................	24	0	20	0.05	1	1	0	0	0	0
...........................................................................................................................................CACCCATTCAGGCCGCCGCCATGC........................................................................................	24	0	20	0.05	1	1	0	0	0	0

CTTCCTTGAGGGTGGAAGATCACCCCTTATCCGACTTCCTGGGGGGCACCCATTCAAGCTGTCAGAGTTCACGCCCTAAATTACGCTCACGCGTTCCTAGACGCTATGACTAGCGTCGATGACTCGAATTCCTGGGGGACACCCATTCAGGCCGCCGCCATGCGCTTAGTCGTTTTCTCATATTTAGTTCCTCGTTCACCCTTTTCCTAGTTCCTAGGCGCCACCTTTACTTCGGGTACCCATTTTGCTCA
********************************************************************************************************************************************.....((((.((....((((.((((..((((....)))).)))))))))).)))).*******************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M044
female body

M058
embryo

V039
embryo

V055
head

V106
head

............TGGAAGATCACCCCTTATCCGACTTC.....................................................................................................................................................................................................................

1.00

..........................TTATCCGACTTCCTGGGGGGCACCCAT......................................................................................................................................................................................................

1.00

........................................................................................................................................................CGCCGCCATGCGCTTAGTCGTT.............................................................................

1.00

...................................................................................................................................................CAGGCCGCCGCCATGCGCTTAGTCGTTT............................................................................

1.00

..............................................................................................................................................CCATTCAGGCCGCCGCCATGCGCTT....................................................................................

0.70

................................................................................TTACGCTCACGCGTTCCTAGACGCTAT................................................................................................................................................

0.50

..........................................................................................................................................................CCGCCATGCGCTTAGTCGTT.............................................................................

0.50

......................................................................................................................................................GCCGCCGCCATGAGCTTAGTCGTT.............................................................................

0.50

.....................................................TCAAGCTGTCAGAGTTCACGCCCTAAAT..........................................................................................................................................................................

0.40

..........................................................................................................................................................................................GTTCCTCGTTCACCCTTTTCCTAGTT.......................................

0.35

.............................................................................................................................................................................................CCTCGTTCACCCTTTTCCTAGTTCCT....................................

0.30

.............................................................................................................................................CCCATTCAGGCCGCCGCCATGCGC......................................................................................

0.30

..................................................................................................AGACGCTATGACTCCCGTTG.....................................................................................................................................

0.25

..........................................................................................................................CTCGAATTCCTGGGGGACATCC...........................................................................................................

0.25

.......................................CGGGGGGCACCCATTCAAGCTGTCAGAG........................................................................................................................................................................................

0.20

.........................................................................................................................................................................................AGTTCCTCGTTCACCCTTTTCCTAGTT.......................................

0.20

..................................................................................................................................................TCAGGCCGCCGCCATGCGCTTAGTC................................................................................

0.20

.....................................................................................................................................GGGGGACACCCATTCAGGCCGCCGCCA...........................................................................................

0.20

.....................................................................CACGCCCTAAATTACGCTCACGCGT.............................................................................................................................................................

0.20

...........................................GGGCACCCATTCAAGCTGTCAG..........................................................................................................................................................................................

0.18

...........................................GGGCACCCATTCAAGCTGTCATAGT.......................................................................................................................................................................................

0.17

............................................................GTCAGAGTTCACGCCCTAAATTACGCT....................................................................................................................................................................

0.17

....................................................................TCACGCCCTAAATTACGCTCACG................................................................................................................................................................

0.17

.............................................................................................................................................CCCATTCAGGCCGCCGCCATGCG.......................................................................................

0.15

........................................................................................................................................................................................TAGTTCCTCGTTCACCCTTTTCCTAGTT.......................................

0.15

.............................................................................................................................................................................................CCTCGTTCACCCTTTTCCTAGTTC......................................

0.15

.............................................................................................................................................CCCATTCAGGCCGCCGCCATG.........................................................................................

0.15

...................................................................................................................................................CAGGCCGCCGCCATGCGCTTAGT.................................................................................

0.15

...................................................................................................................................................CAGGCCGCCGCCATGCGCTTAGTC................................................................................

0.15

...............................................ACCCATTCAAGCTGTCAGAGTTCAC...................................................................................................................................................................................

0.14

............................................GCCACCCATTCAAGCTGTCAGAGT.......................................................................................................................................................................................

0.14

..........................................................................................................................CTCGAAATCCTGTGGGAC...............................................................................................................

0.14

.......................................................AAGCTGTCAGAGTTCACGC.................................................................................................................................................................................

0.13

.......................................TGGGGGGCACCCATTCAAGCTGTCAG..........................................................................................................................................................................................

0.13

.........................................GGGGGCACCCATTCAAGCTGTCAG..........................................................................................................................................................................................

0.11

..........................................................................................................................................ACACCCATTCAGGCCGCCGCCATGC........................................................................................

0.10

............................................................................................................................................................................................TCCTCGTTCACCCTTTTCCTAGTTC......................................

0.10

.........................................GGGGGCACCCATTCAAGCTGT.............................................................................................................................................................................................

0.10

........................................................................................................................................GGACACCCATTCAGGCCGCCGCCATGCGC......................................................................................

0.10

.......................................................................................................................................................................................................CCTTTTCCTAGTTCCTAGGCGCCAC...........................

0.08

......................................................................................................................................................................................................CCCTTTTCCTAGTTCCTAGGCGCCAC...........................

0.08

.......................................................................................................................................................................................TTAGTTCCTCGTTCACCCTTTTCCTAGT........................................

0.05

..........................................................................................................................................ACACCCATTCAGGCCGCCGCCA...........................................................................................

0.05

...........................................................................................................................................................................................TTCCTCGTTCACCCTTTTCCTAGTT.......................................

0.05

......................................................................................................................................GGGGACACCCATTCAGGCCGCCTC.............................................................................................

0.05

..........................................................CTGTCAGAGTTCACGCCCTAAAT..........................................................................................................................................................................

0.05

...........................................................................................................................................CACCCATTCAGGCCGCCGCCATGCGCTT....................................................................................

0.05

...........................................................................................................................................................................................TTCCTCGTTCACCCTTTTCCTAGT........................................

0.05

...........................................................................................................................................................................................TTCCTCGTTCACCCTTATCCTAGTT.......................................

0.05

..............................................................................................................................................................................................CTCGTTCACCCTTTTCCTAGTTCATA...................................

0.05

.........................................................GCTGTCAGAGTTCACGCCCT..............................................................................................................................................................................

0.05

............................................................................................................................................................................................TCCTCGTTCACCCTTTTCCTAGTT.......................................

0.05

.................................................................................................................................................TTCAGGCCGCCGCCATGCGCTTAGTC................................................................................

0.05

..................................................................................................................................................CCAGGCCGCCGCCATGCGCTTAGT.................................................................................

0.05

.................................................................................................................................................TTCAGGCCGCCGCCATGCGCTTAGGC................................................................................

0.05

..............................................................................................................................................CCATTCAGGCCGCCGCCATGCGC......................................................................................

0.05

..........................................................................................................................................................................................GTTCCTCGTTCACCCTTTTCCTAGTTCC.....................................

0.05

............................................................................................................................................ACCCATTCAGGCCGCCGCCATGCGCTTAG..................................................................................

0.05

.....................................................................................................................................GGGGGACACCCATTCAGGCCGCCGCCATG.........................................................................................

0.05

...........................................................................................................................................................................................TTCCTCGTTCACCCTTTTCCT...........................................

0.05

................................................................................................................................................ATTCAGGCCGCCGCCATGCGCTTA...................................................................................

0.05

...........................................................................................................................................CACCCATTCAGGCCGCCGCCATGC........................................................................................

0.05

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
droAna3	scaffold_13250:3358404-3358654 -	dan_1625	GAAGGAACTCCCACCTTCTAGTGGGGAATAGGCTGAAGGACCCCCCGTGGGTAAGTTCGACAGTC------TCAAGTGCGGGATTTAATGCGAGTGCGCAAGGATCTGCGATACTGATCGCAGCTAC-TGAGCTTAAGGACCCCCTGTGGGTAAGTCCGGCGGCGGTACGCGAATCAGCAAAAG---AGTATAAATCAAGGAGCAAGTGGGAAAAGGATCAAGGATCCGCGGTGGAAATG------------AAGCCCATGGGTAAAACGAGT
droBip1	scf7180000396486:4218-4430 +		CTTGTAGCGTT--------------GAGTAGG----AGGACCGCC-TTTGGAAAGTCCGACATTCAAGTGTTCAAGAGCGACATTCAA-----GAGCG-------TTTCTAAAAAGGACGCGGTTATTTGAGTTGAAGGACCGCCTTTGA-AGAGTCCAACA---------------GCCCAAAGAAAGGAGAACGTAAAGAGCGAGTGA---AAG-------GATCCGTTGCAGGA-TGGTTCCCCAGGACAAGCCCAGG-GTATCA-GAGT
droFic1	scf7180000453342:66121-66160 -		CA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCGAAAA---AAAATAAGTAGAGGAGCAAGGGAGGAGAGGA-------------------------------------------------------
droEug1	scf7180000409905:57628-57642 +		GAGGCAACTCCCACC------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droGri2	scaffold_15110:21448829-21448841 +		GGT---ACTCATACCT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droAna3

GAAGGAACTCCCACCTTCTAGTGGGGAATAGGCTGAAGGACCCCCCGTGGGTAAGTTCGACAGTC------TCAAGTGCGGGATTTAATGCGAGTGCGCAAGGATCTGCGATACTGATCGCAGCTAC-TGAGCTTAAGGACCCCCTGTGGGTAAGTCCGGCGGCGGTACGCGAATCAGCAAAAG---AGTATAAATCAAGGAGCAAGTGGGAAAAGGATCAAGGATCCGCGGTGGAAATG------------AAGCCCATGGGTAAAACGAGT	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
............................................................................................................................................................................................................................AAGGATCCGCGGTGGAAATG------------AAGCCC...............	26	2	0.50	1	0	1	0	0	0	0
.............................................................................................GTGCGCAAGGATCTGCGATACTGA............................................................................................................................................................	24	2	0.50	1	1	0	0	0	0	0
...............................................................................................................................................................................................TAAATCAAGGAGCAAGTGGGA.............................................................	21	4	0.25	1	1	0	0	0	0	0
.......................................................TTCGACAGTC------TCAAGTGCGGGA..............................................................................................................................................................................................	22	5	0.20	1	0	1	0	0	0	0
....................................................................................................................................................................................................................AAAGGATCAAGGATCCGCGGTG.......................................	22	13	0.15	2	2	0	0	0	0	0
...................................................................................................................................................................................................................AAAAGGATCAAGGATCCGCGGTGG......................................	24	13	0.08	1	1	0	0	0	0	0
....................................................................................................................................................................................................................AAAGGATCAAGGATCCGCGGTGGAA....................................	25	13	0.08	1	0	1	0	0	0	0
......................................................................................................................................................................................................................AGGATCAAGGATCCGCGGTGGA.....................................	22	15	0.07	1	1	0	0	0	0	0
...................................................................................................................................................................................................................AAAAGGATCAAGGATCCGCGGT........................................	22	15	0.07	1	0	0	0	0	1	0
...........................................................................................................................................................................................................CAAGTGGGAAAAGGATCAAGGATCCGC...........................................	27	20	0.05	1	1	0	0	0	0	0
............................TAGGCTGAAGGACCCCCCGTGGGTAAG..........................................................................................................................................................................................................................	27	20	0.05	1	0	0	1	0	0	0
.............................................................................................................................................................................................................AGTGGGAAAAGGATCAAGGATCC.............................................	23	20	0.05	1	1	0	0	0	0	0
........................................................................................................................................................AAGTCCGGCGGCGGTACGCGAA...................................................................................................	22	20	0.05	1	1	0	0	0	0	0
.....................................................................................................................................TTAAGGACCCCCTGTGGGTAAGTC....................................................................................................................	24	20	0.05	1	0	1	0	0	0	0

droBip1

CTTGTAGCGTT--------------GAGTAGG----AGGACCGCC-TTTGGAAAGTCCGACATTCAAGTGTTCAAGAGCGACATTCAA-----GAGCG-------TTTCTAAAAAGGACGCGGTTATTTGAGTTGAAGGACCGCCTTTGA-AGAGTCCAACA---------------GCCCAAAGAAAGGAGAACGTAAAGAGCGAGTGA---AAG-------GATCCGTTGCAGGA-TGGTTCCCCAGGACAAGCCCAGG-GTATCA-GAGT	Size	Hit Count	Total Norm	Total
No Reads

droFic1

CA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCGAAAA---AAAATAAGTAGAGGAGCAAGGGAGGAGAGGA-------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEug1

GAGGCAACTCCCACC------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droGri2

GGT---ACTCATACCT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

dan_1625

scaffold_13250:3358454-3358604 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-13.1	-13.1	-12.5