dan_129

TTCTCGAACAGTCATCCCGTTATTTTCCCATGCAGTGTCATCGCATTAAAGTCGTCTATCGATGGGTGGCCAGAGTGGGGTGGTGAGCGGTGGGTGGGTGGTAGGTGCTTGGCATGGAATGCGAAAAAGTTGGTTGAGAAAAAATAAGAAAAAAA *********************************(((((..(((((....(((((((((.((.....((((........)))).....)).)))))))))..))))).)))))......*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V106 head	V105 male body	V055 head	M044 female body	V039 embryo
......................................................................................GCGGTGGGTGGGTGGTAG...................................................	18	0	3	118.33	355	350	1	4	0	0
....................................................................................GAGTGGTGGGTGGGTGGTA....................................................	19	1	4	12.50	50	46	4	0	0	0
...........................................................................TGGGGTGGTGAGCGGTGGGT............................................................	20	0	1	12.00	12	12	0	0	0	0
...............................................................................GTGGTGAGCGGTGGGTGGGT........................................................	20	0	1	10.00	10	10	0	0	0	0
.....................................................................................AGCGGTGGGTGGGTGGTAG...................................................	19	0	2	6.00	12	12	0	0	0	0
......................................................................................GCGGTGGGTGGGTGGTAGG..................................................	19	0	3	5.67	17	17	0	0	0	0
...............................................................................GTGGTGAGCGGTGGGTGGG.........................................................	19	0	1	5.00	5	4	1	0	0	0
...........................................................................TGGGGTGGTGAGCGGTGGGTG...........................................................	21	0	1	4.00	4	4	0	0	0	0
.....................................................................................AGTGGTGGGTGGGTGGTA....................................................	18	1	6	3.17	19	16	0	1	2	0
............................................................................GGGGTGGTGAGCGGTGGG.............................................................	18	0	1	3.00	3	3	0	0	0	0
.....................................................................................AGCGGTGGGTGGGTGGTA....................................................	18	0	2	2.50	5	5	0	0	0	0
...........................................................................TGGGGTGGTGAGCGGTGGG.............................................................	19	0	1	2.00	2	2	0	0	0	0
.........................................................................................GTGGGTGGGTGGTAGGTGCA..............................................	20	1	2	2.00	4	4	0	0	0	0
...............................................................................GTGGTGAGCGGTGGGTGGGTGGTAA...................................................	25	1	1	2.00	2	2	0	0	0	0
......................................................................................GCGGTGGGTGGGTTGTAG...................................................	18	1	3	1.33	4	4	0	0	0	0
......................................................................................GCGGTGGGTGGGTGGTAT...................................................	18	1	3	1.33	4	4	0	0	0	0
......................................................................................GCTGTGGGTGGGTGGTAG...................................................	18	1	5	1.20	6	6	0	0	0	0
.........................................................................................GTGGGTGGGTGGTAGGTG................................................	18	0	2	1.00	2	2	0	0	0	0
...............................................................................GTGGTGAGCGGTGGGTGGGTG.......................................................	21	0	1	1.00	1	1	0	0	0	0
......................................................................................GCGGTGGGTGGGTCGTAG...................................................	18	1	3	1.00	3	3	0	0	0	0
...........................................................................TGGGGTGGTGAGCGGTGGGTGT..........................................................	22	1	1	1.00	1	1	0	0	0	0
...............................................................................GTGGTGAGCGGTGGGTGGGA........................................................	20	1	1	1.00	1	1	0	0	0	0
................................................................................TGGTGAGCGGTGGGTGGGT........................................................	19	0	1	1.00	1	1	0	0	0	0
......................................................................................GCGGTGGGTTGGTGGTAG...................................................	18	1	4	1.00	4	4	0	0	0	0
......................................................................................GCGGTGGGGGGGTGGTAG...................................................	18	1	3	1.00	3	3	0	0	0	0
...........................................................................TGGGGTGGTGATCGGTGGGTG...........................................................	21	1	1	1.00	1	1	0	0	0	0
...............................................................................GTGGTGAGCGGTGGGTGGGTGTT.....................................................	23	1	1	1.00	1	1	0	0	0	0
.....................................................................................AGCGGTGGGTGGGTGGTAGAA.................................................	21	2	3	0.67	2	2	0	0	0	0
......................................................................................GCGGTGGGAGGGTGGTAGG..................................................	19	1	4	0.50	2	2	0	0	0	0
..................................................................................................................TGGAATGCTAAGAAGTTTGTTG...................	22	3	2	0.50	1	0	0	1	0	0
....................................................................................GAGCGGTGGGTGGGTGGTAG...................................................	20	0	2	0.50	1	1	0	0	0	0
..................................................................................................................TGGAATGGGAAGAAGTTGGGTG...................	22	3	2	0.50	1	0	0	1	0	0
...............................................................................GTGGTGATCGGTGGGTGGGT........................................................	20	1	2	0.50	1	1	0	0	0	0
.........................................................................................GTGGGTGGGTGGTAGG..................................................	16	0	8	0.50	4	0	0	4	0	0
............................................................................GGGGTGGTGATCGGTGGG.............................................................	18	1	2	0.50	1	1	0	0	0	0
...............................................................................GTGGTGAGCGGTGGGTGGGTT.......................................................	21	1	2	0.50	1	1	0	0	0	0
.....................................................................................AGCGGTGGGTGGGTGGTAGG..................................................	20	0	2	0.50	1	1	0	0	0	0
....................................................................................GAGCGGTGGGTGGGTGGT.....................................................	18	0	2	0.50	1	1	0	0	0	0
.....................................................................................AGCGGTGGGTTGGTGGTAG...................................................	19	1	2	0.50	1	1	0	0	0	0
......................................................................................GCGGTGGGTGGGTGGTAA...................................................	18	1	5	0.40	2	2	0	0	0	0
......................................................................................GCGGTGGGTGGGTTGTAGG..................................................	19	1	3	0.33	1	1	0	0	0	0
......................................................................................GCGGTGGGTGGGTGGCAG...................................................	18	1	3	0.33	1	0	1	0	0	0
......................................................................................GCGGCGGGTGGGTGGTAG...................................................	18	1	3	0.33	1	1	0	0	0	0
.....................................................................................AGGGGTGGGTGGGTGGTA....................................................	18	1	3	0.33	1	1	0	0	0	0
......................................................................................GCGGTGGGCGGGTGGTAG...................................................	18	1	3	0.33	1	1	0	0	0	0
................................................................................TGGTGAGAGGTGGGTGGG.........................................................	18	1	3	0.33	1	1	0	0	0	0
......................................................................................GCGGTGGGTGGGTGGCAGG..................................................	19	1	3	0.33	1	1	0	0	0	0
......................................................................................GCGGTGGGTAGGTGGTAG...................................................	18	1	3	0.33	1	1	0	0	0	0
......................................................................................GCGGTGGGAGGGTGGTAG...................................................	18	1	4	0.25	1	1	0	0	0	0
......................................................................................GCGGTGCGTGGGTGGTAG...................................................	18	1	4	0.25	1	1	0	0	0	0
......................................................................................GCGGTGGGTGGGTGGTAGT..................................................	19	1	4	0.25	1	1	0	0	0	0
......................................................................................GCGGTGGGTGGGCGGTAG...................................................	18	1	4	0.25	1	1	0	0	0	0
............................................................GATGGGTGGTCAGAGTGGC............................................................................	19	2	4	0.25	1	0	1	0	0	0
......................................................................................GCTGTGGGTGGGTGGTAGG..................................................	19	1	4	0.25	1	1	0	0	0	0
...................................................................................AGAGCGGTGGGTGGGTGGT.....................................................	19	1	4	0.25	1	1	0	0	0	0
......................................................................................GCGGTTGGTGGGTGGTAG...................................................	18	1	5	0.20	1	1	0	0	0	0
......................................................................................GCGGTGGGTGTGTGGTAG...................................................	18	1	5	0.20	1	1	0	0	0	0
......................................................................................GCGGTGGGTGGGTGTTAG...................................................	18	1	5	0.20	1	1	0	0	0	0
......................................................................................GCGATGGGTGGGTGGTAG...................................................	18	1	5	0.20	1	1	0	0	0	0
......................................................................................GCGTTGGGTGGGTGGTAG...................................................	18	1	5	0.20	1	1	0	0	0	0
......................................................................................GCGGTGGGTCGGTGGTAG...................................................	18	1	6	0.17	1	1	0	0	0	0
......................................................................................GCGGTGGGTGGGTGGAAG...................................................	18	1	6	0.17	1	1	0	0	0	0
.................................................................GTGGGCGGAGTGGGGTGGTGAA....................................................................	22	3	7	0.14	1	0	1	0	0	0
.................................................................................GATGGGCGGTGGGTTGGTGGTAG...................................................	23	3	7	0.14	1	0	1	0	0	0
...........................................................................TGGGGTGTTGAGAGGTGTGTGG..........................................................	22	3	8	0.13	1	0	0	1	0	0
.............................................................AAGGGTGGCCATAGTGGG............................................................................	18	2	8	0.13	1	1	0	0	0	0
.....................................................................................AGGGTTGGGTGGGTGGTAG...................................................	19	2	9	0.11	1	1	0	0	0	0
....................................................................................GAGTGGTGGGCGGGTGGTA....................................................	19	2	10	0.10	1	1	0	0	0	0
........................................................................GAGGGGTGTGGTGAGCGG.................................................................	18	2	10	0.10	1	0	0	1	0	0
......................................................................................TCGGTGGGTGGGTGGTAG...................................................	18	1	10	0.10	1	1	0	0	0	0
......................................................................................GCGGTGGGTGGGTTGTAT...................................................	18	2	10	0.10	1	1	0	0	0	0
.............................................................................................GTGGGTGGTGGGTGCATGGC..........................................	20	2	10	0.10	1	0	1	0	0	0
.....................................................................................AGGGTTGGGTGGGTGGTA....................................................	18	2	20	0.10	2	2	0	0	0	0
........................................................................................GGTGGGTCAGTGGTAGATGC...............................................	20	3	11	0.09	1	1	0	0	0	0
....................................................................................GAGTTGTGGGTGGGTGGTA....................................................	19	2	11	0.09	1	0	1	0	0	0
...................................................................................AGAGTGGTGGGTGGGTGGTA....................................................	20	2	11	0.09	1	0	1	0	0	0
.....................................................................CATGAGTGGGGTTGTGAGCG..................................................................	20	3	13	0.08	1	0	0	1	0	0
....................................................................................GGGCGGTGGGTTGGTGGTAG...................................................	20	2	14	0.07	1	0	0	0	1	0
..................................................................................................................TGGAATGCGAAAAAGTCT.......................	18	2	16	0.06	1	0	0	1	0	0
........................................................................GAGCGGGGTGGATAGCGGT................................................................	19	3	19	0.05	1	0	0	1	0	0
................................................................................GGGTGAGCGGTGGGGGTGTG.......................................................	20	3	20	0.05	1	0	0	1	0	0
....................................................................................GAGCGGCGGGCGGGTAGTA....................................................	19	3	20	0.05	1	0	0	0	0	1

TTCTCGAACAGTCATCCCGTTATTTTCCCATGCAGTGTCATCGCATTAAAGTCGTCTATCGATGGGTGGCCAGAGTGGGGTGGTGAGCGGTGGGTGGGTGGTAGGTGCTTGGCATGGAATGCGAAAAAGTTGGTTGAGAAAAAATAAGAAAAAAA

***********************************(((((..(((((....(((((((((.((.....((((........)))).....)).)))))))))..))))).)))))......***********************************

Read size

# Mismatch

Hit Count

Total Norm

Total

V106

head

V105

male body

V055

head

M044

female body

V039

embryo

......................................................................................GCGGTGGGTGGGTGGTAG...................................................

118.33

355

350

....................................................................................GAGTGGTGGGTGGGTGGTA....................................................

12.50

...........................................................................TGGGGTGGTGAGCGGTGGGT............................................................

12.00

...............................................................................GTGGTGAGCGGTGGGTGGGT........................................................

10.00

.....................................................................................AGCGGTGGGTGGGTGGTAG...................................................

6.00

......................................................................................GCGGTGGGTGGGTGGTAGG..................................................

5.67

...............................................................................GTGGTGAGCGGTGGGTGGG.........................................................

5.00

...........................................................................TGGGGTGGTGAGCGGTGGGTG...........................................................

4.00

.....................................................................................AGTGGTGGGTGGGTGGTA....................................................

3.17

............................................................................GGGGTGGTGAGCGGTGGG.............................................................

3.00

.....................................................................................AGCGGTGGGTGGGTGGTA....................................................

2.50

...........................................................................TGGGGTGGTGAGCGGTGGG.............................................................

2.00

.........................................................................................GTGGGTGGGTGGTAGGTGCA..............................................

2.00

...............................................................................GTGGTGAGCGGTGGGTGGGTGGTAA...................................................

2.00

......................................................................................GCGGTGGGTGGGTTGTAG...................................................

1.33

......................................................................................GCGGTGGGTGGGTGGTAT...................................................

1.33

......................................................................................GCTGTGGGTGGGTGGTAG...................................................

1.20

.........................................................................................GTGGGTGGGTGGTAGGTG................................................

1.00

...............................................................................GTGGTGAGCGGTGGGTGGGTG.......................................................

1.00

......................................................................................GCGGTGGGTGGGTCGTAG...................................................

1.00

...........................................................................TGGGGTGGTGAGCGGTGGGTGT..........................................................

1.00

...............................................................................GTGGTGAGCGGTGGGTGGGA........................................................

1.00

................................................................................TGGTGAGCGGTGGGTGGGT........................................................

1.00

......................................................................................GCGGTGGGTTGGTGGTAG...................................................

1.00

......................................................................................GCGGTGGGGGGGTGGTAG...................................................

1.00

...........................................................................TGGGGTGGTGATCGGTGGGTG...........................................................

1.00

...............................................................................GTGGTGAGCGGTGGGTGGGTGTT.....................................................

1.00

.....................................................................................AGCGGTGGGTGGGTGGTAGAA.................................................

0.67

......................................................................................GCGGTGGGAGGGTGGTAGG..................................................

0.50

..................................................................................................................TGGAATGCTAAGAAGTTTGTTG...................

0.50

....................................................................................GAGCGGTGGGTGGGTGGTAG...................................................

0.50

..................................................................................................................TGGAATGGGAAGAAGTTGGGTG...................

0.50

...............................................................................GTGGTGATCGGTGGGTGGGT........................................................

0.50

.........................................................................................GTGGGTGGGTGGTAGG..................................................

0.50

............................................................................GGGGTGGTGATCGGTGGG.............................................................

0.50

...............................................................................GTGGTGAGCGGTGGGTGGGTT.......................................................

0.50

.....................................................................................AGCGGTGGGTGGGTGGTAGG..................................................

0.50

....................................................................................GAGCGGTGGGTGGGTGGT.....................................................

0.50

.....................................................................................AGCGGTGGGTTGGTGGTAG...................................................

0.50

......................................................................................GCGGTGGGTGGGTGGTAA...................................................

0.40

......................................................................................GCGGTGGGTGGGTTGTAGG..................................................

0.33

......................................................................................GCGGTGGGTGGGTGGCAG...................................................

0.33

......................................................................................GCGGCGGGTGGGTGGTAG...................................................

0.33

.....................................................................................AGGGGTGGGTGGGTGGTA....................................................

0.33

......................................................................................GCGGTGGGCGGGTGGTAG...................................................

0.33

................................................................................TGGTGAGAGGTGGGTGGG.........................................................

0.33

......................................................................................GCGGTGGGTGGGTGGCAGG..................................................

0.33

......................................................................................GCGGTGGGTAGGTGGTAG...................................................

0.33

......................................................................................GCGGTGGGAGGGTGGTAG...................................................

0.25

......................................................................................GCGGTGCGTGGGTGGTAG...................................................

0.25

......................................................................................GCGGTGGGTGGGTGGTAGT..................................................

0.25

......................................................................................GCGGTGGGTGGGCGGTAG...................................................

0.25

............................................................GATGGGTGGTCAGAGTGGC............................................................................

0.25

......................................................................................GCTGTGGGTGGGTGGTAGG..................................................

0.25

...................................................................................AGAGCGGTGGGTGGGTGGT.....................................................

0.25

......................................................................................GCGGTTGGTGGGTGGTAG...................................................

0.20

......................................................................................GCGGTGGGTGTGTGGTAG...................................................

0.20

......................................................................................GCGGTGGGTGGGTGTTAG...................................................

0.20

......................................................................................GCGATGGGTGGGTGGTAG...................................................

0.20

......................................................................................GCGTTGGGTGGGTGGTAG...................................................

0.20

......................................................................................GCGGTGGGTCGGTGGTAG...................................................

0.17

......................................................................................GCGGTGGGTGGGTGGAAG...................................................

0.17

.................................................................GTGGGCGGAGTGGGGTGGTGAA....................................................................

0.14

.................................................................................GATGGGCGGTGGGTTGGTGGTAG...................................................

0.14

...........................................................................TGGGGTGTTGAGAGGTGTGTGG..........................................................

0.13

.............................................................AAGGGTGGCCATAGTGGG............................................................................

0.13

.....................................................................................AGGGTTGGGTGGGTGGTAG...................................................

0.11

....................................................................................GAGTGGTGGGCGGGTGGTA....................................................

0.10

........................................................................GAGGGGTGTGGTGAGCGG.................................................................

0.10

......................................................................................TCGGTGGGTGGGTGGTAG...................................................

0.10

......................................................................................GCGGTGGGTGGGTTGTAT...................................................

0.10

.............................................................................................GTGGGTGGTGGGTGCATGGC..........................................

0.10

.....................................................................................AGGGTTGGGTGGGTGGTA....................................................

0.10

........................................................................................GGTGGGTCAGTGGTAGATGC...............................................

0.09

....................................................................................GAGTTGTGGGTGGGTGGTA....................................................

0.09

...................................................................................AGAGTGGTGGGTGGGTGGTA....................................................

0.09

.....................................................................CATGAGTGGGGTTGTGAGCG..................................................................

0.08

....................................................................................GGGCGGTGGGTTGGTGGTAG...................................................

0.07

..................................................................................................................TGGAATGCGAAAAAGTCT.......................

0.06

........................................................................GAGCGGGGTGGATAGCGGT................................................................

0.05

................................................................................GGGTGAGCGGTGGGGGTGTG.......................................................

0.05

....................................................................................GAGCGGCGGGCGGGTAGTA....................................................

0.05

AAGAGCTTGTCAGTAGGGCAATAAAAGGGTACGTCACAGTAGCGTAATTTCAGCAGATAGCTACCCACCGGTCTCACCCCACCACTCGCCACCCACCCACCATCCACGAACCGTACCTTACGCTTTTTCAACCAACTCTTTTTTATTCTTTTTTT *********************************(((((..(((((....(((((((((.((.....((((........)))).....)).)))))))))..))))).)))))......*********************************	Read size	# Mismatch	Hit Count	Total Norm	Total	V039 embryo	M058 embryo	M044 female body	V105 male body
..................................CACAATAGCGTCATTCCAG......................................................................................................	19	3	5	0.20	1	1	0	0	0
.......................................................................TCTCACCGCACCACTTGACA................................................................	20	3	7	0.14	1	1	0	0	0
..............................................................ACCCTACTGTCTCACCCCA..........................................................................	19	3	17	0.12	2	1	0	1	0
...............................................TTACCGCAGATAGCCACCC.........................................................................................	19	3	12	0.08	1	1	0	0	0
.........TCAGTAGGGGCATAAAACG...............................................................................................................................	19	3	20	0.05	1	0	0	0	1
................................................................................ACCGCTAGCCGCCCACCCAC.......................................................	20	3	20	0.05	1	0	1	0	0
..........................................................................................ACCCACCCAACATCCACAAT.............................................	20	3	20	0.05	1	0	1	0	0
.............................................................................................................ACCGTACCTTACGTCTTTTG..........................	20	3	20	0.05	1	1	0	0	0

AAGAGCTTGTCAGTAGGGCAATAAAAGGGTACGTCACAGTAGCGTAATTTCAGCAGATAGCTACCCACCGGTCTCACCCCACCACTCGCCACCCACCCACCATCCACGAACCGTACCTTACGCTTTTTCAACCAACTCTTTTTTATTCTTTTTTT

***********************************(((((..(((((....(((((((((.((.....((((........)))).....)).)))))))))..))))).)))))......***********************************

Read size

# Mismatch

Hit Count

Total Norm

Total

V039

embryo

M058

embryo

M044

female body

V105

male body

..................................CACAATAGCGTCATTCCAG......................................................................................................

0.20

.......................................................................TCTCACCGCACCACTTGACA................................................................

0.14

..............................................................ACCCTACTGTCTCACCCCA..........................................................................

0.12

...............................................TTACCGCAGATAGCCACCC.........................................................................................

0.08

.........TCAGTAGGGGCATAAAACG...............................................................................................................................

0.05

................................................................................ACCGCTAGCCGCCCACCCAC.......................................................

0.05

..........................................................................................ACCCACCCAACATCCACAAT.............................................

0.05

.............................................................................................................ACCGTACCTTACGTCTTTTG..........................

0.05

Species	Coordinate	ID	Type	Alignment
droAna3	scaffold_13117:3534682-3534836 -	dan_129	candidate	TTCTCGAACAGTCATCCCGTTATTTTCCCATGCAGTGTCATCGCATTAAAGTCGTCTATCGATGGGTGGCCAGAGTGG-----------GGTGGTGAGCGGTGGGTGGGTGGTAGGTGCTTGGCATGGAATGCGAAAAAGTTGGTTGAGAAAAAATAAGAAAAAAA
droBip1	scf7180000395895:22126-22271 +			TTCTCCAACAGTCATCCCGTTATTTTCCCATGCACTGTCATCGCATTAAAGTCGTCTATCGATGGGTGG-----GTGGGCCGCCGAATGGGTGGTGA-------CTGGGTGG--------TGGCATGGAATGCGAAAAAGTTGGTTGAAAAAGAACAAAAAAAAAA

Species

Coordinate

Type

Alignment

droAna3

scaffold_13117:3534682-3534836 -

dan_129

candidate

TTCTCGAACAGTCATCCCGTTATTTTCCCATGCAGTGTCATCGCATTAAAGTCGTCTATCGATGGGTGGCCAGAGTGG-----------GGTGGTGAGCGGTGGGTGGGTGGTAGGTGCTTGGCATGGAATGCGAAAAAGTTGGTTGAGAAAAAATAAGAAAAAAA

droBip1

scf7180000395895:22126-22271 +

TTCTCCAACAGTCATCCCGTTATTTTCCCATGCACTGTCATCGCATTAAAGTCGTCTATCGATGGGTGG-----GTGGGCCGCCGAATGGGTGGTGA-------CTGGGTGG--------TGGCATGGAATGCGAAAAAGTTGGTTGAAAAAGAACAAAAAAAAAA

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

droAna3

TTCTCGAACAGTCATCCCGTTATTTTCCCATGCAGTGTCATCGCATTAAAGTCGTCTATCGATGGGTGGCCAGAGTGG-----------GGTGGTGAGCGGTGGGTGGGTGGTAGGTGCTTGGCATGGAATGCGAAAAAGTTGGTTGAGAAAAAATAAGAAAAAAA	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
.................................................................................................GCGGTGGGTGGGTGGTAG...................................................	18	3	118.33	355	0	0	0	4	1	350
...........................................................................TGG-----------GGTGGTGAGCGGTGGGT............................................................	20	1	12.00	12	0	0	0	0	0	12
..........................................................................................GTGGTGAGCGGTGGGTGGGT........................................................	20	1	10.00	10	0	0	0	0	0	10
................................................................................................AGCGGTGGGTGGGTGGTAG...................................................	19	2	6.00	12	0	0	0	0	0	12
.................................................................................................GCGGTGGGTGGGTGGTAGG..................................................	19	3	5.67	17	0	0	0	0	0	17
..........................................................................................GTGGTGAGCGGTGGGTGGG.........................................................	19	1	5.00	5	0	0	0	0	1	4
...........................................................................TGG-----------GGTGGTGAGCGGTGGGTG...........................................................	21	1	4.00	4	0	0	0	0	0	4
............................................................................GG-----------GGTGGTGAGCGGTGGG.............................................................	18	1	3.00	3	0	0	0	0	0	3
................................................................................................AGCGGTGGGTGGGTGGTA....................................................	18	2	2.50	5	0	0	0	0	0	5
...........................................................................TGG-----------GGTGGTGAGCGGTGGG.............................................................	19	1	2.00	2	0	0	0	0	0	2
....................................................................................................GTGGGTGGGTGGTAGGTGCA..............................................	20	2	2.00	4	0	0	0	0	0	4
..........................................................................................GTGGTGAGCGGTGGGTGGGTGGTAA...................................................	25	1	2.00	2	0	0	0	0	0	2
.................................................................................................GCGGTGGGTGGGTGGTAT...................................................	18	3	1.33	4	0	0	0	0	0	4
....................................................................................................GTGGGTGGGTGGTAGGTG................................................	18	2	1.00	2	0	0	0	0	0	2
..........................................................................................GTGGTGAGCGGTGGGTGGGTG.......................................................	21	1	1.00	1	0	0	0	0	0	1
...........................................................................TGG-----------GGTGGTGAGCGGTGGGTGT..........................................................	22	1	1.00	1	0	0	0	0	0	1
..........................................................................................GTGGTGAGCGGTGGGTGGGA........................................................	20	1	1.00	1	0	0	0	0	0	1
...........................................................................................TGGTGAGCGGTGGGTGGGT........................................................	19	1	1.00	1	0	0	0	0	0	1
................................................................................................AGCGGTGGGTGGGTGGTAGAA.................................................	21	3	0.67	2	0	0	0	0	0	2
...............................................................................................GAGCGGTGGGTGGGTGGTAG...................................................	20	2	0.50	1	0	0	0	0	0	1
....................................................................................................GTGGGTGGGTGGTAGG..................................................	16	8	0.50	4	0	0	0	4	0	0
..........................................................................................GTGGTGAGCGGTGGGTGGGTT.......................................................	21	2	0.50	1	0	0	0	0	0	1
................................................................................................AGCGGTGGGTGGGTGGTAGG..................................................	20	2	0.50	1	0	0	0	0	0	1
...............................................................................................GAGCGGTGGGTGGGTGGT.....................................................	18	2	0.50	1	0	0	0	0	0	1
.................................................................................................GCGGTGGGTGGGTGGTAA...................................................	18	5	0.40	2	0	0	0	0	0	2
.................................................................................................GCGGTGGGTGGGTGGTAGT..................................................	19	4	0.25	1	0	0	0	0	0	1
.............................................................................................................................TGGAATGCGAAAAAGTCT.......................	18	16	0.06	1	0	0	0	1	0	0

droBip1

TTCTCCAACAGTCATCCCGTTATTTTCCCATGCACTGTCATCGCATTAAAGTCGTCTATCGATGGGTGG-----GTGGGCCGCCGAATGGGTGGTGA-------CTGGGTGG--------TGGCATGGAATGCGAAAAAGTTGGTTGAAAAAGAACAAAAAAAAAA	Size	Hit Count	Total Norm	Total
No Reads

dan_129

scaffold_13117:3534732-3534786 -

candidate

Canonical miRNA

intron

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-21.8	-21.5	-21.4