ID:

dan_100

 Coordinate:

scaffold_12903:550549-550608 +

 Confidence:

confident

 Class:

Testes-restricted

 Genomic Locale:

antisense_to_intron

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-31.2 -31.2 -30.9

Flybase annnotation

Antisense to intron [Dana\GF15947-in]

No Repeatable elements found

Sense Strand Reads

AAAAAATTTTTAAAAAGTGATAATAATTCTTGGAGTATTATCTAATTATCAGTAGAAGCCAAAAGGATGTGAGTATTTTTGAAATCCACCTCCCTTTGGCTTCTACTACTTAATTGGTAATCAAAACTGCTTCAAAAACAGTCTAAAACTAACTGAAAAG

***********************************.((((.(((((((..(((((((((((((.(((.(((...............))).))).)))))))))))))...)))))))))))....*********************************** 		Read size # Mismatch Hit Count Total Norm Total M044

female body

 V105

male body		 V106

head

 V055

head

 M058

embryo
........................................................................................CCTCCCTTTGGCTTCTACTACT.................................................. 22 0 1 99.00 99 96 1 0 0 2
........................................................................................CCTCCCTTTGGCTTCTACTAC................................................... 21 0 1 36.00 36 32 1 2 1 0
..................................................AGTAGAAGCCAAAAGGATGTG......................................................................................... 21 0 1 26.00 26 4 11 9 1 1
.........................................................................................CTCCCTTTGGCTTCTACTACT.................................................. 21 0 1 6.00 6 6 0 0 0 0
.........................................................................................CTCCCTTTGGCTTCTACTAC................................................... 20 0 1 5.00 5 5 0 0 0 0
........................................................................................CCTCCCTTTGGCTTCTACTA.................................................... 20 0 1 5.00 5 4 0 1 0 0
.................................................CAGTAGAAGCCAAAAGGATGTG......................................................................................... 22 0 1 5.00 5 0 4 1 0 0
........................................................................................CCTCCCTTTGGCTTCTACTACTT................................................. 23 0 1 3.00 3 3 0 0 0 0
........................................................................................CCTCCCTTGGGCTTCTACTACT.................................................. 22 1 1 3.00 3 3 0 0 0 0
..................................................AGTAGAAGCCAAAAGGATG........................................................................................... 19 0 1 2.00 2 0 1 1 0 0
........................................................................................CCTCCCTTTGGCTTCTACTACTA................................................. 23 1 1 2.00 2 2 0 0 0 0
........................................................................................CCTCCCTTGGGCTTCTACTAC................................................... 21 1 1 2.00 2 2 0 0 0 0
..................................................AGTAGAAGCCAAAAGGATGTGTT....................................................................................... 23 2 1 2.00 2 0 1 1 0 0
..................................................AGTAGAAGCCAAAAGGATGTC......................................................................................... 21 1 1 2.00 2 0 0 1 1 0
..................................................AGTAGAAGCCAAAAGAATGTG......................................................................................... 21 1 1 1.00 1 0 1 0 0 0
........................................................................................CCTCCCTTCGGCTTCTACTA.................................................... 20 1 1 1.00 1 1 0 0 0 0
...................................................GTAGAAGCCAAAAGGATGTG......................................................................................... 20 0 1 1.00 1 1 0 0 0 0
........................................................................................CGTCCCTTTGGCTTCTACTAC................................................... 21 1 1 1.00 1 0 0 0 0 1
........................................................................................CCTCCCTTTGGCTTCTACTACC.................................................. 22 1 1 1.00 1 0 0 0 0 1
..................................................AGTAGAAGCCAAAAGGATGTA......................................................................................... 21 1 1 1.00 1 0 1 0 0 0
..................................................GGTAGAAGCCAAAAGGATGTG......................................................................................... 21 1 1 1.00 1 0 0 1 0 0
........................................................................................CCTCCCTTTGGCTTCAACTACT.................................................. 22 1 1 1.00 1 1 0 0 0 0
........................................................................................CCTCCCTTAGGCTTCTACTACT.................................................. 22 1 1 1.00 1 1 0 0 0 0
..................................................AGTAGAAGCCAAAAGGATGTGTTT...................................................................................... 24 2 1 1.00 1 0 1 0 0 0
.................................................CAGTAGAAGCCAAAAGGATG........................................................................................... 20 0 1 1.00 1 0 0 1 0 0
........................................................................................ACTCCCTTTGGCTTCTACTAC................................................... 21 1 1 1.00 1 0 0 0 1 0
........................................................................................TCTCCCTTTGGCTTCTACTACT.................................................. 22 1 1 1.00 1 0 0 0 1 0
..................................................AGTAGAAGGCAAAAGGATGTG......................................................................................... 21 1 1 1.00 1 0 1 0 0 0
..................................................AGTAGAAGCCAAAAGGATGT.......................................................................................... 20 0 1 1.00 1 0 0 0 1 0
.....................................................................................................................................AAAAACAGTCAAAAGCTAAC....... 20 2 4 0.25 1 0 0 0 0 1

Anti-sense strand reads

TTTTTTAAAAATTTTTCACTATTATTAAGAACCTCATAATAGATTAATAGTCATCTTCGGTTTTCCTACACTCATAAAAACTTTAGGTGGAGGGAAACCGAAGATGATGAATTAACCATTAGTTTTGACGAAGTTTTTGTCAGATTTTGATTGACTTTTC

***********************************.((((.(((((((..(((((((((((((.(((.(((...............))).))).)))))))))))))...)))))))))))....*********************************** 		Read size # Mismatch Hit Count Total Norm Total V106

head

 V105

male body		 M044

female body

 M058

embryo

 V055

head
.....................................................................................GGTGGAGGGAAACCGAAGATGA..................................................... 22 0 1 24.00 24 11 9 4 0 0
.....................................................................................GGTGGAGGGAAACCGAAGATG...................................................... 21 0 1 4.00 4 1 1 2 0 0
........................................................................................GGAGGGAAACCGAAGATGA..................................................... 19 0 1 4.00 4 2 2 0 0 0
........................................................................................TGAGGGAAACCGAAGATGA..................................................... 19 1 1 4.00 4 3 1 0 0 0
.................................................GTCATCTTCGGTTTTCCTACA.......................................................................................... 21 0 1 3.00 3 0 0 2 1 0
........................................................................................GGAGGGAAACCGAAGATGAT.................................................... 20 0 1 3.00 3 0 3 0 0 0
.....................................................................................TGTGGAGGGAAACCGAAGATGA..................................................... 22 1 1 2.00 2 1 0 0 0 1
.....................................................................................TGTGGAGGGAAACCTAAGATGA..................................................... 22 2 1 2.00 2 2 0 0 0 0
......................................................................................GTGGAGGGAAACCGAAGATGA..................................................... 21 0 1 1.00 1 0 1 0 0 0
............................................................................................GGAAACCGAAGATGATGAA................................................. 19 0 1 1.00 1 0 0 0 1 0
.....................................................................................GGTTGAGGGAAACCGAAGATGA..................................................... 22 1 1 1.00 1 0 0 1 0 0
......................................................................................TTTGAGGGAAACCGAAGATGA..................................................... 21 2 1 1.00 1 1 0 0 0 0
.......................................................................................TGGAGGGAAACCGAAGATGA..................................................... 20 0 1 1.00 1 0 0 1 0 0
................................................AGTCATCTTCGGTTTTCCTACA.......................................................................................... 22 0 1 1.00 1 0 0 1 0 0
.....................................................................................GGTGGAGGGAAACCGAAGATGAT.................................................... 23 0 1 1.00 1 1 0 0 0 0
................................................................................................................TAACCATTAGTTTTGACGAAGTTT........................ 24 0 1 1.00 1 0 1 0 0 0
.......................................................................................TGGAGGGAAACGGAAGATGA..................................................... 20 1 1 1.00 1 0 0 1 0 0
.....................................................TCTTCGGTTTTCCTACACTCAGA.................................................................................... 23 1 1 1.00 1 0 0 0 1 0
........................................................................................TGAGGGAGACCGAAGATGA..................................................... 19 2 3 0.33 1 0 1 0 0 0
...........................................................................AAAAAGTTTAGGTGGAAG................................................................... 18 2 20 0.25 5 0 0 0 5 0

Re-alignment of all predicted orthologs

Species Coordinate ID Type Alignment
droAna3 scaffold_12903:550499-550658 + dan_100 confident AAAAAATTTTTAAAAAGTGATAATAATTCTT-----------------------------GGAGTATTATCTAATTAT--CAGTAGAAGCCAAAAGGATGTGAGTATTTTTGAAATCCA----------CCTCCCTTTGGCTTCTACTACTTAATTGGTAATCAAAACTGCTTCAAAAACAGT---------------------CTAAAACTAACTGAAAAG
droBip1 scf7180000395912:19103-19310 + AAAAAAGTCTTCAAA-----TTAAAGTTCAAAGAGAAGAAAAGTGTTAAAATACAAAAAAAAAGTATTATCTAATTACTACAAGGGGAGTCATAGTGAAATACATATTTTTAAAAACTATTACATTATACCTTCCTTTGG---------CTTAATTGATAATCAAATCTTCAACAAAAAAAATAATAATAATAATAATAATAATAAAAAGTGAAAAATGAAG

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
droAna3
AAAAAATTTTTAAAAAGTGATAATAATTCTT-----------------------------GGAGTATTATCTAATTAT--CAGTAGAAGCCAAAAGGATGTGAGTATTTTTGAAATCCA----------CCTCCCTTTGGCTTCTACTACTTAATTGGTAATCAAAACTGCTTCAAAAACAGT---------------------CTAAAACTAACTGAAAAGSizeHit CountTotal NormTotalM044

Female-body		M058

Embryo		V039

Embryo		V055

Head		V105

Male-body		V106

Head
.................................................................................................................................CCTCCCTTTGGCTTCTACTACT.......................................................................22199.00999620010
.................................................................................................................................CCTCCCTTTGGCTTCTACTAC........................................................................21136.00363200112
.................................................................................AGTAGAAGCCAAAAGGATGTG........................................................................................................................21126.00264101119
..................................................................................................................................CTCCCTTTGGCTTCTACTACT.......................................................................2116.006600000
..................................................................................................................................CTCCCTTTGGCTTCTACTAC........................................................................2015.005500000
.................................................................................................................................CCTCCCTTTGGCTTCTACTA.........................................................................2015.005400001
................................................................................CAGTAGAAGCCAAAAGGATGTG........................................................................................................................2215.005000041
.................................................................................................................................CCTCCCTTTGGCTTCTACTACTT......................................................................2313.003300000
.................................................................................AGTAGAAGCCAAAAGGATG..........................................................................................................................1912.002000011
.................................................................................................................................CCTCCCTTTGGCTTCTACTACTA......................................................................2312.002200000
.................................................................................AGTAGAAGCCAAAAGGATGTGTT......................................................................................................................2312.002000011
.................................................................................AGTAGAAGCCAAAAGGATGTC........................................................................................................................2112.002000101
..................................................................................GTAGAAGCCAAAAGGATGTG........................................................................................................................2011.001100000
.................................................................................................................................CCTCCCTTTGGCTTCTACTACC.......................................................................2211.001010000
.................................................................................AGTAGAAGCCAAAAGGATGTA........................................................................................................................2111.001000010
................................................................................CAGTAGAAGCCAAAAGGATG..........................................................................................................................2011.001000001
.................................................................................AGTAGAAGCCAAAAGGATGT.........................................................................................................................2011.001000100
droBip1
AAAAAAGTCTTCAAA-----TTAAAGTTCAAAGAGAAGAAAAGTGTTAAAATACAAAAAAAAAGTATTATCTAATTACTACAAGGGGAGTCATAGTGAAATACATATTTTTAAAAACTATTACATTATACCTTCCTTTGG---------CTTAATTGATAATCAAATCTTCAACAAAAAAAATAATAATAATAATAATAATAATAAAAAGTGAAAAATGAAGSizeHit CountTotal NormTotal
No Reads

Generated: 10/20/2015 at 06:45 PM