dps_991

##################################################--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- ATCTGTAGCCGGGAGAGCGACAGTACGGATAGCCCTTCCTCGCGAGGCCTGTGACTCTCAGACTTTTTTCATCGCGACGCCAGTGACTCTTAGACCTTTTGCTTCGCGACGCCAGTGACTGTGAGACCTTTTTCTTCGCAAGGCCAGTGACTGTAAGACCGAGGCCTGTGATTCTTCAACCTTTTACTGGATCTACTTTCAATGGCAAATGCTTTCAAGGCCCAAAACTGATTAAAACCAGGATTTAAATT ***************************************************************************************...........((((((......((((.((((.....((((.........)))).)))).))))......))))))..............**********************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR902011 testis	GSM444067 head	M040 female body
.........................................................................................................................................................TAAGACCGAGGCCTGTGATTCTTCAA........................................................................	26	0	1	2.00	2	2	0	0
.........................................................................................TTAGACCTTTTGCTTCGCGACGCC..........................................................................................................................................	24	0	1	2.00	2	2	0	0
..................................................................................................................................................GTGACTGTAAGACCGAGGC......................................................................................	19	0	1	1.00	1	1	0	0
.......................TACGGATAGCCCTTCCTCGCGAGGCC..........................................................................................................................................................................................................	26	0	1	1.00	1	0	1	0
..............................................................................................................................................................................TTCAACCTTGTTCTGGAT...........................................................	18	2	7	0.14	1	0	0	1
...........................................................................................................................................................TGACGGAGGCCTGTGGTTCT............................................................................	20	3	9	0.11	1	0	0	1

##################################################---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
ATCTGTAGCCGGGAGAGCGACAGTACGGATAGCCCTTCCTCGCGAGGCCTGTGACTCTCAGACTTTTTTCATCGCGACGCCAGTGACTCTTAGACCTTTTGCTTCGCGACGCCAGTGACTGTGAGACCTTTTTCTTCGCAAGGCCAGTGACTGTAAGACCGAGGCCTGTGATTCTTCAACCTTTTACTGGATCTACTTTCAATGGCAAATGCTTTCAAGGCCCAAAACTGATTAAAACCAGGATTTAAATT
*****************************************************************************************...........((((((......((((.((((.....((((.........)))).)))).))))......))))))..............************************************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

SRR902011
testis

GSM444067
head

M040
female body

.........................................................................................................................................................TAAGACCGAGGCCTGTGATTCTTCAA........................................................................

2.00

.........................................................................................TTAGACCTTTTGCTTCGCGACGCC..........................................................................................................................................

2.00

..................................................................................................................................................GTGACTGTAAGACCGAGGC......................................................................................

1.00

.......................TACGGATAGCCCTTCCTCGCGAGGCC..........................................................................................................................................................................................................

1.00

..............................................................................................................................................................................TTCAACCTTGTTCTGGAT...........................................................

0.14

...........................................................................................................................................................TGACGGAGGCCTGTGGTTCT............................................................................

0.11

TAGACATCGGCCCTCTCGCTGTCATGCCTATCGGGAAGGAGCGCTCCGGACACTGAGAGTCTGAAAAAAGTAGCGCTGCGGTCACTGAGAATCTGGAAAACGAAGCGCTGCGGTCACTGACACTCTGGAAAAAGAAGCGTTCCGGTCACTGACATTCTGGCTCCGGACACTAAGAAGTTGGAAAATGACCTAGATGAAAGTTACCGTTTACGAAAGTTCCGGGTTTTGACTAATTTTGGTCCTAAATTTAA **********************************************************************...........((((((......((((.((((.....((((.........)))).)))).))))......))))))..............***************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M040 female body	V112 male body	SRR902011 testis
....................................................................................................................................AGAAGCGTTTCGGTGACTG....................................................................................................	19	2	1	1.00	1	1	0	0
.....................................................................................................................................................................................................CAGGTACCGTTTACGAAAG...................................	19	2	2	0.50	1	1	0	0
...............................CGGGAAGGTGCGCTCTGG..........................................................................................................................................................................................................	18	2	3	0.33	1	1	0	0
...............................................................AAAAAATTTGCGCTGCGGT.........................................................................................................................................................................	19	2	3	0.33	1	0	1	0
...........................CTACCGGGAAGGTGCGCT..............................................................................................................................................................................................................	18	2	5	0.20	1	1	0	0
............................TACCGGGAAGGTGCGCTCT............................................................................................................................................................................................................	19	3	10	0.10	1	1	0	0
....................................................................................................................................................................................................ACAGGAACCGTTTACGAAAG...................................	20	3	20	0.05	1	0	0	1
...........................CTAGCGGGAAGGTGCGCC..............................................................................................................................................................................................................	18	3	20	0.05	1	0	1	0
...............................................................AAAACATTTGCGCTGCGGT.........................................................................................................................................................................	19	3	20	0.05	1	1	0	0
.........................................................................................GGTCTGGAAAACGAAGCGG...............................................................................................................................................	19	3	20	0.05	1	1	0	0
..............................CCGGGAAGGTGCGCTCTG...........................................................................................................................................................................................................	18	3	20	0.05	1	1	0	0

TAGACATCGGCCCTCTCGCTGTCATGCCTATCGGGAAGGAGCGCTCCGGACACTGAGAGTCTGAAAAAAGTAGCGCTGCGGTCACTGAGAATCTGGAAAACGAAGCGCTGCGGTCACTGACACTCTGGAAAAAGAAGCGTTCCGGTCACTGACATTCTGGCTCCGGACACTAAGAAGTTGGAAAATGACCTAGATGAAAGTTACCGTTTACGAAAGTTCCGGGTTTTGACTAATTTTGGTCCTAAATTTAA
************************************************************************...........((((((......((((.((((.....((((.........)))).)))).))))......))))))..............*****************************************************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M040
female body

V112
male body

SRR902011
testis

....................................................................................................................................AGAAGCGTTTCGGTGACTG....................................................................................................

1.00

.....................................................................................................................................................................................................CAGGTACCGTTTACGAAAG...................................

0.50

...............................CGGGAAGGTGCGCTCTGG..........................................................................................................................................................................................................

0.33

...............................................................AAAAAATTTGCGCTGCGGT.........................................................................................................................................................................

0.33

...........................CTACCGGGAAGGTGCGCT..............................................................................................................................................................................................................

0.20

............................TACCGGGAAGGTGCGCTCT............................................................................................................................................................................................................

0.10

....................................................................................................................................................................................................ACAGGAACCGTTTACGAAAG...................................

0.05

...........................CTAGCGGGAAGGTGCGCC..............................................................................................................................................................................................................

0.05

...............................................................AAAACATTTGCGCTGCGGT.........................................................................................................................................................................

0.05

.........................................................................................GGTCTGGAAAACGAAGCGG...............................................................................................................................................

0.05

..............................CCGGGAAGGTGCGCTCTG...........................................................................................................................................................................................................

0.05

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
dp5	3:3147753-3148003 +	dps_991	ATCTGTAGCCGGGAGAGCGACAGTACGGATAGCCCTTCCTCGCGAGGCCTGTGACTCTCAGACTTTTTTCATCGCGACGCCAGTGACTCTTAGACCTTTTGCTTCGCGACGCCAGTGACTGTGAGACCTTTTTCTTCGCAAGGCCAGTGACTGTAAGACCGAGGCCTGTGATTCTTCAACCTTTTACTGGATCTACTTTCAATGGCAAATGCTTTCAAGGCCCAAAACTGATTAAAACCAGGATTTAAATT
droPer2	scaffold_2:3341281-3341531 +		ATCTGTAGCCGGGAGAGCGACAGTACGGATAGCCCTTCCTCGCGAGGCCTGTGACTGTGAGACCTGTTTCATCGCGACGCCAGTGACTGTCAGACCTTTTGCTTCGCGACGCCAGTGACTGTCAGACCTTTTTCTTCGCAAGGCCAGTGACTGTAAGACCGAGGCCTGTGATTATTCAACCTTTTACTGGATCTACTTTCAATGGCAAATGGTTTCAAGGCCCAAAACTGATTAAAACCAGGATTTAAATT

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

dp5

ATCTGTAGCCGGGAGAGCGACAGTACGGATAGCCCTTCCTCGCGAGGCCTGTGACTCTCAGACTTTTTTCATCGCGACGCCAGTGACTCTTAGACCTTTTGCTTCGCGACGCCAGTGACTGTGAGACCTTTTTCTTCGCAAGGCCAGTGACTGTAAGACCGAGGCCTGTGATTCTTCAACCTTTTACTGGATCTACTTTCAATGGCAAATGCTTTCAAGGCCCAAAACTGATTAAAACCAGGATTTAAATT	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
.........................................................................................................................................................TAAGACCGAGGCCTGTGATTCTTCAA........................................................................	26	1	2.00	2	0	0	0	0	0	0	0	0	2	0
.........................................................................................TTAGACCTTTTGCTTCGCGACGCC..........................................................................................................................................	24	1	2.00	2	0	0	0	0	0	0	0	0	2	0
..................................................................................................................................................GTGACTGTAAGACCGAGGC......................................................................................	19	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.......................TACGGATAGCCCTTCCTCGCGAGGCC..........................................................................................................................................................................................................	26	1	1.00	1	0	0	0	0	0	1	0	0	0	0

droPer2

ATCTGTAGCCGGGAGAGCGACAGTACGGATAGCCCTTCCTCGCGAGGCCTGTGACTGTGAGACCTGTTTCATCGCGACGCCAGTGACTGTCAGACCTTTTGCTTCGCGACGCCAGTGACTGTCAGACCTTTTTCTTCGCAAGGCCAGTGACTGTAAGACCGAGGCCTGTGATTATTCAACCTTTTACTGGATCTACTTTCAATGGCAAATGGTTTCAAGGCCCAAAACTGATTAAAACCAGGATTTAAATT	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
No Reads

dps_991

3:3147803-3147953 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-19.0	-18.2	-18.2