dps_681

-----------------------------------###############--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- ATTGAAAGAGCAAAAACAAAAGACCAACTCGAACCATGTCACGGACCAAGGTGAGCAATAAACTCTTTAAAACGAAATGGTAGTTCGTCCAGAACATTTTATTGTAACTGAAATCGGAATATACATATGGCTGCCAGTTTGAATTCTATTTATGTTGCGATGATGTCAACTTTAATGGCATAATCGGATTGGCAGTAGTTTTCTGGTGAGGTAAAATAATTAAGGGCTTGTAAATCATGGGAAACCTATGA ************************************************....((((((((.........(((((.......))))).........))))))))..(((....)))............(((((((((((((((.(((((...((((((....)).))))...))))).))..))))))))))))).....************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M040 female body	GSM343916 embryo	M059 embryo	V112 male body	GSM444067 head	M062 head
.........GCAAAAACAAAAGACCAACT..............................................................................................................................................................................................................................	20	0	1	3.00	3	3	0	0	0	0	0
...................................................................................................................................................................................................TAGTTAGTTGGTGAGGTAAA....................................	20	3	8	2.25	18	6	0	0	12	0	0
.........................................................................................................................................................GTTGCGATGATGTCAACTTTAATGGC........................................................................	26	0	1	1.00	1	0	0	1	0	0	0
.........................AACTCGAACCATGTCACGGA..............................................................................................................................................................................................................	20	0	1	1.00	1	0	0	1	0	0	0
...........................................................................................................................................................TGCGATGATGTCAACTTTAAT...........................................................................	21	0	1	1.00	1	1	0	0	0	0	0
.......................................................................................................................................................................AACTTTAATGGCATAATCGGATTTGC..........................................................	26	1	1	1.00	1	0	0	0	1	0	0
.............................CGAACCATGTCACGGACCAAGC........................................................................................................................................................................................................	22	1	1	1.00	1	1	0	0	0	0	0
...................................................................................................................................................................................ATAATCGGATTGGCAGTAGTTT..................................................	22	0	1	1.00	1	1	0	0	0	0	0
.................AAAAGACCAACTCGAACCATGT....................................................................................................................................................................................................................	22	0	1	1.00	1	0	1	0	0	0	0
.........GCAAAAACAAAAGACCAACTCGAAC.........................................................................................................................................................................................................................	25	0	1	1.00	1	1	0	0	0	0	0
...........................................................................................................................................TGAATCCTATCTTTGTTGCGA...........................................................................................	21	3	1	1.00	1	0	1	0	0	0	0
.......GAGCAAAAACAAAAGACCAAC...............................................................................................................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0
............................................................................................................................................................GCGATGATGTCAACTTTAATGGCAT......................................................................	25	0	1	1.00	1	1	0	0	0	0	0
........AGCAAAAACAAAAGACCAACT..............................................................................................................................................................................................................................	21	0	1	1.00	1	0	1	0	0	0	0
........................................................................................................................................................................ACTTTAATGGCATAATCGGATTGGC..........................................................	25	0	1	1.00	1	0	0	1	0	0	0
.....................................................................................................................................................................................................................................GTAACACGTGGGAAACCT....	18	3	20	0.60	12	8	0	0	0	3	1
...............................................................................................................................................................ATGATGTCAACTTTAATTACAGA.....................................................................	23	3	2	0.50	1	0	1	0	0	0	0
..........................................................................ATATGGTAGTTCGTCTAGAAG............................................................................................................................................................	21	3	7	0.14	1	0	0	0	0	1	0
..............................................................................................................................AAGGCTGCTAGCTTGAATT..........................................................................................................	19	3	20	0.10	2	2	0	0	0	0	0
...................................................................................................................................................................................................TAGTTAGTTGGTGAGGTAA.....................................	19	3	20	0.10	2	0	0	0	2	0	0
......................................................................................................................................................................................................TTATCTGGGGAGGTAAAAAA.................................	20	3	11	0.09	1	1	0	0	0	0	0
.........................AACCCGAACCATGTGATGGA..............................................................................................................................................................................................................	20	3	12	0.08	1	1	0	0	0	0	0
.......................................................................................................................ACTACATCTGGCTGCCAGTT................................................................................................................	20	3	13	0.08	1	0	1	0	0	0	0
........................................ACGGACCAAGGAGAGAAA.................................................................................................................................................................................................	18	2	14	0.07	1	1	0	0	0	0	0

TAACTTTCTCGTTTTTGTTTTCTGGTTGAGCTTGGTACAGTGCCTGGTTCCACTCGTTATTTGAGAAATTTTGCTTTACCATCAAGCAGGTCTTGTAAAATAACATTGACTTTAGCCTTATATGTATACCGACGGTCAAACTTAAGATAAATACAACGCTACTACAGTTGAAATTACCGTATTAGCCTAACCGTCATCAAAAGACCACTCCATTTTATTAATTCCCGAACATTTAGTACCCTTTGGATACT ************************************************....((((((((.........(((((.......))))).........))))))))..(((....)))............(((((((((((((((.(((((...((((((....)).))))...))))).))..))))))))))))).....************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M040 female body
..................................................................................................................................GACGGTCAAACTTAAGATAAA....................................................................................................	21	0	1	1.00	1	1
.................................................................................................................................................................................................TCATCAAACGACCGCACCA.......................................	19	3	20	0.05	1	1

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
dp5	2:20945681-20945931 +	dps_681	ATTGAAAGAGCAAAAACA-A----AAGACCAACTC--------GAACCATGTCACGGACCAAGGTGAGCAATAAACTCTTTAAA---ACGAAATGGTAGTTCGTCCAGAACATTTTATTGTAACTGAAATCGGAATATACATATGGCTGCCAGTTTGAATTCT--------ATTTATGTTGCGATGATGTCAACTTTAATGGCATAATCGGATTGGCAGTAGTTTTCTGGTGAGGTAAAATAATTAAGGGCTTGTAAATCATGGGAAACCTATGA
droPer2	scaffold_3:3695767-3696017 +		ATTGAAAGAGCAAAAACA-A----AAGACCCACTC--------GAACCATGTCACGGACCAAGGTGAGCAATAAACTCTTTAAA---ACGAAATGGTAGTTCGTCCAGAACATTTTATTGTAACTGAAATCGGAATATACATATGGCTGCCAGTTTGAATTCT--------ATTTATGTTCCGATGATGTCAACTTTAATGGCATAATCGGATTGGCAGTAGTTTTCTGGTGAGGTAAAATAATTAAGGGCTTGTAAATCATGGGAAACCTATGA
droBip1	scf7180000396708:2779089-2779162 -		ACCATAAAATCGGAACCA-ACCGCAAAATCCGCCAAAACTATACGATAATGGCGCATTCCAAGGTGAGTAATAAA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droKik1	scf7180000302706:1105917-1105978 -		AACCCAAGAA-----CC-G-----CAAAGCCGCCAAA--CACTCGATAATGCCGCATTCCGAGGTGAGTAATGAA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droFic1	scf7180000454104:395534-395595 -		AA---GCGAG-----CC-GA----AGAAACCGCCAAA--CACTCAATAATGCCGCATTCCAAGGTGAGTAATAAACT------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEle1	scf7180000486474:1014123-1014175 -		GA----------------------AGAAACCGCCAAA--CACTCGATAATGCCGCATTCCAAGGTGAGTAATAAACT------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droRho1	scf7180000779540:35291-35349 +		C------GA-----ACC-AA----CGAAACCGCCAAA--CACTTGATAATGCCGCATTCCAAGGTGAGTAACAAGCT------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEug1	scf7180000409794:30554-30663 -		AA-------------CC-GA----AGAAACCGACAAA--CATTCGATAATGCCGCATTCCAAGGTGAGTAATAAACAGCCGGGATCACCGAAGTCACAGAATTTCTATTACATTTAATGCTAATTGGAAT-------------------------------------------------------------------------------------------------------------------------------------------------
droEre2	scaffold_4770:10491690-10491852 +		AA------------ACC-AA----AGGGTCCGCCAGA--CATTCGATAATGCCACATTCCGAGGTCAGTAATAAATTCGCGAAT---TCGCGAAAAAAATTCAAGGCAATTGGTTTATTTTTACT-----------------ATTGCTATCAGTGAGTGTTCTGAACTTTGGCTGATGGTCCGATAGTAGCCAATTTAATGG-------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

dp5

ATTGAAAGAGCAAAAACA-A----AAGACCAACTC--------GAACCATGTCACGGACCAAGGTGAGCAATAAACTCTTTAAA---ACGAAATGGTAGTTCGTCCAGAACATTTTATTGTAACTGAAATCGGAATATACATATGGCTGCCAGTTTGAATTCT--------ATTTATGTTGCGATGATGTCAACTTTAATGGCATAATCGGATTGGCAGTAGTTTTCTGGTGAGGTAAAATAATTAAGGGCTTGTAAATCATGGGAAACCTATGA	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
.........GCAAAAACA-A----AAGACCAACT.................................................................................................................................................................................................................................................	20	1	3.00	3	0	3	0	0	0	0	0	0	0	0
.................................................................................................................................................................................GTTGCGATGATGTCAACTTTAATGGC........................................................................	26	1	1.00	1	0	0	1	0	0	0	0	0	0	0
..............................AACTC--------GAACCATGTCACGGA.........................................................................................................................................................................................................................	20	1	1.00	1	0	0	1	0	0	0	0	0	0	0
...................................................................................................................................................................................TGCGATGATGTCAACTTTAAT...........................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..................................C--------GAACCATGTCACGGACCAAGC...................................................................................................................................................................................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...........................................................................................................................................................................................................ATAATCGGATTGGCAGTAGTTT..................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.................A-A----AAGACCAACTC--------GAACCATGT...............................................................................................................................................................................................................................	22	1	1.00	1	0	0	0	0	1	0	0	0	0	0
.........GCAAAAACA-A----AAGACCAACTC--------GAAC....................................................................................................................................................................................................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.......GAGCAAAAACA-A----AAGACCAAC..................................................................................................................................................................................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0
....................................................................................................................................................................................GCGATGATGTCAACTTTAATGGCAT......................................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0
........AGCAAAAACA-A----AAGACCAACT.................................................................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0	0	0	0	0
................................................................................................................................................................................................ACTTTAATGGCATAATCGGATTGGC..........................................................	25	1	1.00	1	0	0	1	0	0	0	0	0	0	0

droPer2

ATTGAAAGAGCAAAAACA-A----AAGACCCACTC--------GAACCATGTCACGGACCAAGGTGAGCAATAAACTCTTTAAA---ACGAAATGGTAGTTCGTCCAGAACATTTTATTGTAACTGAAATCGGAATATACATATGGCTGCCAGTTTGAATTCT--------ATTTATGTTCCGATGATGTCAACTTTAATGGCATAATCGGATTGGCAGTAGTTTTCTGGTGAGGTAAAATAATTAAGGGCTTGTAAATCATGGGAAACCTATGA	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
........................................................................................................................................................................................................GGCATAATCGGATTGGCA.........................................................	18	1	3.00	3	3	0	0	0	0	0
.................................................................................................................................................................................................................GGATTGGCAGTAGTTTTCTGGT............................................	22	1	2.00	2	0	2	0	0	0	0
...............................................................................................................................................................................................AACTTTAATGGCATAATCGGATTGGCA.........................................................	27	1	2.00	2	2	0	0	0	0	0
..............AACA-A----AAGACCCACTC--------GAACC...................................................................................................................................................................................................................................	21	1	2.00	2	2	0	0	0	0	0
.................A-A----AAGACCCACTC--------GAACCATGTCACGGAC........................................................................................................................................................................................................................	29	1	2.00	2	2	0	0	0	0	0
.................................................................................................................................................................................................CTTTAATGGCATAATCGGATTGGCA.........................................................	25	1	1.00	1	1	0	0	0	0	0
..................................C--------GAACCATGTCACGGACC.......................................................................................................................................................................................................................	18	1	1.00	1	1	0	0	0	0	0
.............................................ACCATGTCACGGACCAAG....................................................................................................................................................................................................................	18	1	1.00	1	1	0	0	0	0	0
................................CTC--------GAACCATGTCACGGACC.......................................................................................................................................................................................................................	20	1	1.00	1	1	0	0	0	0	0
.....................................................................................................................................................................................................AATGGCATAATCGGATTGGCA.........................................................	21	1	1.00	1	1	0	0	0	0	0
.................A-A----AAGACCCACTC--------GAACCATGTCAC............................................................................................................................................................................................................................	25	1	1.00	1	1	0	0	0	0	0

droBip1

ACCATAAAATCGGAACCA-ACCGCAAAATCCGCCAAAACTATACGATAATGGCGCATTCCAAGGTGAGTAATAAA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droKik1

AACCCAAGAA-----CC-G-----CAAAGCCGCCAAA--CACTCGATAATGCCGCATTCCGAGGTGAGTAATGAA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droFic1

AA---GCGAG-----CC-GA----AGAAACCGCCAAA--CACTCAATAATGCCGCATTCCAAGGTGAGTAATAAACT------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEle1

GA----------------------AGAAACCGCCAAA--CACTCGATAATGCCGCATTCCAAGGTGAGTAATAAACT------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droRho1

C------GA-----ACC-AA----CGAAACCGCCAAA--CACTTGATAATGCCGCATTCCAAGGTGAGTAACAAGCT------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEug1

AA-------------CC-GA----AGAAACCGACAAA--CATTCGATAATGCCGCATTCCAAGGTGAGTAATAAACAGCCGGGATCACCGAAGTCACAGAATTTCTATTACATTTAATGCTAATTGGAAT-------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEre2

AA------------ACC-AA----AGGGTCCGCCAGA--CATTCGATAATGCCACATTCCGAGGTCAGTAATAAATTCGCGAAT---TCGCGAAAAAAATTCAAGGCAATTGGTTTATTTTTACT-----------------ATTGCTATCAGTGAGTGTTCTGAACTTTGGCTGATGGTCCGATAGTAGCCAATTTAATGG-------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
..............................CGCCAGA--CATTCGATAATG................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	1	0	0	0
.............................CCGCCAGA--CATTCGATAAT.................................................................................................................................................................................................................................	19	1	1.00	1	0	0	0	0	0	1	0
...............CC-AA----AGGGTCCGCCAGA--CATTCGA.....................................................................................................................................................................................................................................	24	1	1.00	1	0	0	0	0	1	0	0
AA------------ACC-AA----AGGGTCCGCCAGA--CA..........................................................................................................................................................................................................................................	22	1	1.00	1	1	0	0	0	0	0	0
...........................................CGATAATGCCACATTCCGAGG...................................................................................................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0	0
.....................................................ACATTCCGAGGTCAGTAATAAATTCGC...................................................................................................................................................................................................	27	1	1.00	1	0	0	0	0	0	1	0
.................................CAGA--CATTCGATAATGCCACATT.........................................................................................................................................................................................................................	23	1	1.00	1	1	0	0	0	0	0	0
..............ACC-AA----AGGGTCCGCCAGA--CATTCGAT....................................................................................................................................................................................................................................	26	1	1.00	1	0	0	0	1	0	0	0
.A------------ACC-AA----AGGGTCCGCCAGA--CATTCGAT....................................................................................................................................................................................................................................	27	1	1.00	1	1	0	0	0	0	0	0
................................CCAGA--CATTCGATAATGCCACA...........................................................................................................................................................................................................................	22	1	1.00	1	1	0	0	0	0	0	0
.................................CAGA--CATTCGATAATGCCACAT..........................................................................................................................................................................................................................	22	1	1.00	1	1	0	0	0	0	0	0
.............................................................................................AAAAAATTCAAGGCAATTGGT.................................................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0	0
.........................................TTCGATAATGCCACATTCCGAGC...................................................................................................................................................................................................................	23	1	1.00	1	0	1	0	0	0	0	0

dps_681

2:20945731-20945881 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-31.3	-31.1	-31.1