ID:

dps_42

 Coordinate:

XL_group1a:4093812-4093885 +

 Confidence:

candidate

 Class:

Canonical miRNA

 Genomic Locale:

CDS

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-36.9 -36.8 -36.5 -36.3

Flybase annnotation

CDS [Dpse\GA22983-cds]

No Repeatable elements found

mature

  1. 2:12450392-12450412 -
  2. dps_42  XL_group1a:4093864-4093884 +
star

Sense Strand Reads

GCATTGCGGCTGTGGTGGAAGTCGGAATGAAGGCCTACCGTTATTTCCGGCCACCGCCCCCTGAGCCATCGGCTGTCTTCGTCTGGAAGTCCATCTGCACCCACTGGGTGGGGGTCCTGGCTGGGCCCGTCATACTGGCGCTGATTTTCAAGCGCACCTACGACATCATCAAGG

************************************.((.......((((((...((((((....(((..((.(((((((.....)))).......))).))..)))..))))))...))))))............))************************************ 		Read size # Mismatch Hit Count Total Norm Total V112

male body		 SRR902011

testis

 M040

female body

 GSM444067

head

 M022

male body		 M062

head

 GSM343916

embryo
......................................................................................................ACTGGGTGGGGGTCCTGGCTG................................................... 21 0 2 25.00 50 32 3 8 7 0 0 0
.....................................................................................................CACTGGGTGGGGGTCCTGGC..................................................... 20 0 2 16.50 33 26 2 2 1 0 2 0
......................................................................................................ACTGGGTGGGGGTCCTGGC..................................................... 19 0 2 9.50 19 15 0 1 2 0 1 0
........................................................................................................TGGGTGGGGGTCCTGGCTGGG................................................. 21 0 2 7.00 14 11 0 3 0 0 0 0
...............TGGAAGTCGGAATGAAGGC............................................................................................................................................ 19 0 1 7.00 7 0 7 0 0 0 0 0
..................................................................................................ACCCACTGGGTGGGGGTCCTG....................................................... 21 0 1 7.00 7 0 0 4 0 2 1 0
............TGGTGGAAGTCGGAATGAAGGC............................................................................................................................................ 22 0 1 6.00 6 0 6 0 0 0 0 0
..............GTGGAAGTCGGAATGAAGGC............................................................................................................................................ 20 0 1 5.00 5 0 5 0 0 0 0 0
.....................................................................................................CACTGGGTGGGGGTCCTGG...................................................... 19 0 2 4.50 9 5 2 1 1 0 0 0
...............TGGAAGTCGGAATGAAGGCC........................................................................................................................................... 20 0 1 4.00 4 0 4 0 0 0 0 0
......CGGCTGTGGTGGAAGTCGGA.................................................................................................................................................... 20 0 1 3.00 3 3 0 0 0 0 0 0
..............GTGGAAGTCGGAATGAAGGCC........................................................................................................................................... 21 0 1 3.00 3 0 3 0 0 0 0 0
........................................................................................................TGGGTGGGGGTCCTGGCTG................................................... 19 0 2 2.50 5 3 0 2 0 0 0 0
...................................................................................................CCCACTGGGTGGGGGTCCTGG...................................................... 21 0 2 2.50 5 0 4 1 0 0 0 0
...................................................CACCGCCCCCTGAGCCATC........................................................................................................ 19 0 1 2.00 2 0 0 2 0 0 0 0
........GCTGTGGTGGAAGTCGGAATGAAGG............................................................................................................................................. 25 0 1 2.00 2 0 2 0 0 0 0 0
........................................................................................................TGGGTGGGGGTCCTGGCTGG.................................................. 20 0 2 2.00 4 2 0 1 1 0 0 0
............TGGTGGAAGTCGGAATGAAGG............................................................................................................................................. 21 0 1 2.00 2 0 2 0 0 0 0 0
....TGCGGCTGTGGTGGAAGTCGG..................................................................................................................................................... 21 0 1 2.00 2 2 0 0 0 0 0 0
......................................................................................................ACTGGGTGGGGGTCCTGGCTA................................................... 21 1 2 1.50 3 2 0 1 0 0 0 0
......................................................................................................ACTGGGTGGGGGTCCTGGCT.................................................... 20 0 2 1.50 3 3 0 0 0 0 0 0
...............TGGAAGTCGGAATGAAGGCCTA......................................................................................................................................... 22 0 1 1.00 1 0 1 0 0 0 0 0
................GGAAGTCGGAATGAAGGC............................................................................................................................................ 18 0 1 1.00 1 0 1 0 0 0 0 0
..................................................CCACCGCCCCCTGAGCCATCG....................................................................................................... 21 0 1 1.00 1 1 0 0 0 0 0 0
........GCTGTGGTGGAAGTCGGAATG................................................................................................................................................. 21 0 1 1.00 1 0 1 0 0 0 0 0
............TGGTGGAAGTCGGAATAAAGGC............................................................................................................................................ 22 1 1 1.00 1 0 1 0 0 0 0 0
............TGGCGGAAGTCGGAATGAAGGC............................................................................................................................................ 22 1 1 1.00 1 0 1 0 0 0 0 0
....................................................................................................CCACTGGGTGGGGGTCCTGG...................................................... 20 0 2 1.00 2 1 0 1 0 0 0 0
.......................................................................................................CTGGGTGGGGGTCCTGGCTGG.................................................. 21 0 2 1.00 2 2 0 0 0 0 0 0
.............................AAGGCCCACCGTTATTTCCGG............................................................................................................................ 21 1 1 1.00 1 0 1 0 0 0 0 0
........................................................CCCCCTGAGCCATCGGCTGTC................................................................................................. 21 0 1 1.00 1 0 0 1 0 0 0 0
.............GGTGGAAGTCGGAATGGAGG............................................................................................................................................. 20 1 1 1.00 1 0 1 0 0 0 0 0
....................................................................................................CCACTGGGTGGGGGTCCTGGC..................................................... 21 0 2 1.00 2 1 0 1 0 0 0 0
..............................AGGCCTACCGTTATTTCCG............................................................................................................................. 19 0 1 1.00 1 0 1 0 0 0 0 0
........GCTGTGGTGGAAGTCGGA.................................................................................................................................................... 18 0 1 1.00 1 0 1 0 0 0 0 0
.........................................................................................................GGGTGGGGGTCCTGGCTGGG................................................. 20 0 2 1.00 2 0 0 1 1 0 0 0
......................................................................................................ACTGGGTGGGGGTCCTGG...................................................... 18 0 2 1.00 2 1 0 1 0 0 0 0
..............GTGGAAGTCGGAATGAAGG............................................................................................................................................. 19 0 1 1.00 1 0 1 0 0 0 0 0
.............................AAGGCCTACCGTTATTTCCGG............................................................................................................................ 21 0 1 1.00 1 0 1 0 0 0 0 0
..............................................................................................CTGCACCCACTGGGTGGG.............................................................. 18 0 2 0.50 1 0 0 0 0 1 0 0
..........................................................................................................GGTGGGGGTCCTGGCTGGGC................................................ 20 0 2 0.50 1 1 0 0 0 0 0 0
.......................................................................................................CTGGGTGGGGGTCCTGGC..................................................... 18 0 2 0.50 1 1 0 0 0 0 0 0
......................................................................................................ACTGGGTGGGGGTCCTGGCTGG.................................................. 22 0 2 0.50 1 0 0 1 0 0 0 0
...................................................................................................CCCACTGGGTGGGGGTCCTG....................................................... 20 0 2 0.50 1 0 0 1 0 0 0 0
..........................................................................................................GGTGGGGGTCCTGGCTGGGTTCT............................................. 23 3 2 0.50 1 1 0 0 0 0 0 0
.................................................................................................CACCCACTGGGTGGGGGTCCCA....................................................... 22 2 2 0.50 1 0 0 1 0 0 0 0
.....................................................................................................CACTGGGTGGGGGTCCTGGCT.................................................... 21 0 2 0.50 1 1 0 0 0 0 0 0
.....................................................................................................CACTGGGTGGGGGTCCTGGCTGGGG................................................ 25 1 2 0.50 1 1 0 0 0 0 0 0
.....................................................................................................CACTGGGTGGGGGTCCTGGCA.................................................... 21 1 2 0.50 1 1 0 0 0 0 0 0
......................................................................................................ACTGGGTGGGGGTCCTGGCTC................................................... 21 1 2 0.50 1 1 0 0 0 0 0 0
..............................................................................................CTGCACCCACTGGGTGGGGG............................................................ 20 0 2 0.50 1 0 0 1 0 0 0 0
..................................................CCACCGCCCCCTGAGCCACCG....................................................................................................... 21 1 3 0.33 1 1 0 0 0 0 0 0
.....................................................................................................CACTGGGTGGGGGTCCTG....................................................... 18 0 3 0.33 1 0 0 1 0 0 0 0
.....................................................................................................CACTGGGTGGGGCTCCTG....................................................... 18 1 5 0.20 1 0 0 0 0 1 0 0
....TGCGGGTGTGGTGGAATT........................................................................................................................................................ 18 2 9 0.11 1 0 0 1 0 0 0 0
..........................................ATTCCCGGCCGCCGCCCC.................................................................................................................. 18 2 11 0.09 1 1 0 0 0 0 0 0
.....GCGGGTGTGGTGGAATTGGGA.................................................................................................................................................... 21 3 12 0.08 1 1 0 0 0 0 0 0
..........................................................................................CCATCTACACCGACAGGGT................................................................. 19 3 18 0.06 1 0 0 0 0 0 0 1
...TCGCGGGTGTGGTGGAATT........................................................................................................................................................ 19 3 20 0.05 1 0 0 1 0 0 0 0
..CTTGCGGGTGTGGTGGAAT......................................................................................................................................................... 19 3 20 0.05 1 0 0 1 0 0 0 0

Anti-sense strand reads

CGTAACGCCGACACCACCTTCAGCCTTACTTCCGGATGGCAATAAAGGCCGGTGGCGGGGGACTCGGTAGCCGACAGAAGCAGACCTTCAGGTAGACGTGGGTGACCCACCCCCAGGACCGACCCGGGCAGTATGACCGCGACTAAAAGTTCGCGTGGATGCTGTAGTAGTTCC

************************************.((.......((((((...((((((....(((..((.(((((((.....)))).......))).))..)))..))))))...))))))............))************************************ 		Read size # Mismatch Hit Count Total Norm Total M040

female body

 SRR902011

testis

 GSM444067

head

 M022

male body		 M062

head

 V112

male body
....................................................................................................GGTGACCCACCCCCAGGACC...................................................... 20 0 2 4.00 8 1 6 1 0 0 0
....................................................................................................GGTGACCCACCCCCAGGAC....................................................... 19 0 2 1.50 3 2 0 0 1 0 0
.......................................................................................................GACCCACCCCCAGGACCGAC................................................... 20 0 2 1.50 3 2 1 0 0 0 0
.....................................................................................................GTGACCCACCCCCAGGACC...................................................... 19 0 2 1.00 2 2 0 0 0 0 0
..........................................................................................................CCACCCCCAGGACCGACCCG................................................ 20 0 2 0.50 1 0 0 0 0 1 0
....................................................................................................GGTGACCCACCCCCAGGACCG..................................................... 21 0 2 0.50 1 1 0 0 0 0 0
.................................................CGGTGGCTGGGGACTCGGTAGC....................................................................................................... 22 1 3 0.33 1 0 0 1 0 0 0
..............................................GGCCGGTGGCTGGGGACTCGG........................................................................................................... 21 1 3 0.33 1 0 0 0 0 0 1
..............................................GGCCGGTGGCTGGGGACTC............................................................................................................. 19 1 3 0.33 1 0 0 1 0 0 0
.................................................CGGTGGCGGGGGACTCGGTG......................................................................................................... 20 1 3 0.33 1 0 1 0 0 0 0
...AACGCCGACACTACCTTGAGC...................................................................................................................................................... 21 2 6 0.17 1 1 0 0 0 0 0
........................................................................................................ACCCACCCCCTGGACCTA.................................................... 18 2 11 0.09 1 1 0 0 0 0 0
................................................CTGGTGGCTGGGGACTCGG........................................................................................................... 19 2 20 0.05 1 0 0 0 0 0 1

Re-alignment of all predicted orthologs

Species Coordinate ID Type Alignment
dp5 XL_group1a:4093762-4093935 + dps_42 candidate GCATTGCGGCTGTGGTGGAAGTCGGAATGAAGGCCTACCGTTATTTCCGGCCACCGCCCCCTGAGCCATCGGCTGTCTTCGTCTGGAAGTCCATCTGCACCCACTGGGTGGGGGTCCTGGCTGGGCCCGTCATACTGGCGCTGATTTTCAAGCGCACCTACGACATCATCAAGG
droPer2 scaffold_11:660767-660940 + GCATTGCGGCTGTGGTGGAAGTCGGAATGAAGGCCTACCGTTATTTCCGGCCACCGCCCCCTGAGCCATCGGCCGTCTTCGTCTGGAAGTCCATCTGCACCCACTGGGTGGGGGTCCTGGCTGGGCCCGTCATACTGGCGCTGATTTTCAAGCGCACCTACGACATCATCAAGG

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
dp5
GCATTGCGGCTGTGGTGGAAGTCGGAATGAAGGCCTACCGTTATTTCCGGCCACCGCCCCCTGAGCCATCGGCTGTCTTCGTCTGGAAGTCCATCTGCACCCACTGGGTGGGGGTCCTGGCTGGGCCCGTCATACTGGCGCTGATTTTCAAGCGCACCTACGACATCATCAAGGSizeHit CountTotal NormTotalM022

Male-body		M040

Female-body		M059

Embryo		M062

Head		V043

Embryo		V051

Head		V112

Male-body		SRR902010

Ovary		SRR902011

Testis		SRR902012

CNS imaginal disc
......................................................................................................ACTGGGTGGGGGTCCTGGCTG...................................................21225.005008000732030
.....................................................................................................CACTGGGTGGGGGTCCTGGC.....................................................20216.503302020126020
......................................................................................................ACTGGGTGGGGGTCCTGGC.....................................................1929.501901010215000
........................................................................................................TGGGTGGGGGTCCTGGCTGGG.................................................2127.001403000011000
...............TGGAAGTCGGAATGAAGGC............................................................................................................................................1917.0070000000070
..................................................................................................ACCCACTGGGTGGGGGTCCTG.......................................................2117.0072401000000
............TGGTGGAAGTCGGAATGAAGGC............................................................................................................................................2216.0060000000060
..............GTGGAAGTCGGAATGAAGGC............................................................................................................................................2015.0050000000050
.....................................................................................................CACTGGGTGGGGGTCCTGG......................................................1924.5090100015020
...............TGGAAGTCGGAATGAAGGCC...........................................................................................................................................2014.0040000000040
......CGGCTGTGGTGGAAGTCGGA....................................................................................................................................................2013.0030000003000
..............GTGGAAGTCGGAATGAAGGCC...........................................................................................................................................2113.0030000000030
........................................................................................................TGGGTGGGGGTCCTGGCTG...................................................1922.5050200003000
...................................................................................................CCCACTGGGTGGGGGTCCTGG......................................................2122.5050100000040
...................................................CACCGCCCCCTGAGCCATC........................................................................................................1912.0020200000000
........GCTGTGGTGGAAGTCGGAATGAAGG.............................................................................................................................................2512.0020000000020
........................................................................................................TGGGTGGGGGTCCTGGCTGG..................................................2022.0040100012000
............TGGTGGAAGTCGGAATGAAGG.............................................................................................................................................2112.0020000000020
....TGCGGCTGTGGTGGAAGTCGG.....................................................................................................................................................2112.0020000002000
......................................................................................................ACTGGGTGGGGGTCCTGGCTA...................................................2121.5030100002000
......................................................................................................ACTGGGTGGGGGTCCTGGCT....................................................2021.5030000003000
...............TGGAAGTCGGAATGAAGGCCTA.........................................................................................................................................2211.0010000000010
................GGAAGTCGGAATGAAGGC............................................................................................................................................1811.0010000000010
..................................................CCACCGCCCCCTGAGCCATCG.......................................................................................................2111.0010000001000
........GCTGTGGTGGAAGTCGGAATG.................................................................................................................................................2111.0010000000010
....................................................................................................CCACTGGGTGGGGGTCCTGG......................................................2021.0020100001000
.......................................................................................................CTGGGTGGGGGTCCTGGCTGG..................................................2121.0020000002000
........................................................CCCCCTGAGCCATCGGCTGTC.................................................................................................2111.0010100000000
....................................................................................................CCACTGGGTGGGGGTCCTGGC.....................................................2121.0020100001000
..............................AGGCCTACCGTTATTTCCG.............................................................................................................................1911.0010000000010
........GCTGTGGTGGAAGTCGGA....................................................................................................................................................1811.0010000000010
.........................................................................................................GGGTGGGGGTCCTGGCTGGG.................................................2021.0020100010000
......................................................................................................ACTGGGTGGGGGTCCTGG......................................................1821.0020100001000
..............GTGGAAGTCGGAATGAAGG.............................................................................................................................................1911.0010000000010
.............................AAGGCCTACCGTTATTTCCGG............................................................................................................................2111.0010000000010
..............................................................................................CTGCACCCACTGGGTGGG..............................................................1820.5011000000000
..........................................................................................................GGTGGGGGTCCTGGCTGGGC................................................2020.5010000001000
.......................................................................................................CTGGGTGGGGGTCCTGGC.....................................................1820.5010000001000
......................................................................................................ACTGGGTGGGGGTCCTGGCTGG..................................................2220.5010100000000
...................................................................................................CCCACTGGGTGGGGGTCCTG.......................................................2020.5010100000000
.................................................................................................CACCCACTGGGTGGGGGTCCCA.......................................................2220.5010100000000
.....................................................................................................CACTGGGTGGGGGTCCTGGCT....................................................2120.5010000001000
.....................................................................................................CACTGGGTGGGGGTCCTGGCTGGGG................................................2520.5010000001000
.....................................................................................................CACTGGGTGGGGGTCCTGGCA....................................................2120.5010000001000
......................................................................................................ACTGGGTGGGGGTCCTGGCTC...................................................2120.5010000001000
..............................................................................................CTGCACCCACTGGGTGGGGG............................................................2020.5010100000000
.....................................................................................................CACTGGGTGGGGGTCCTG.......................................................1830.3310100000000
droPer2
GCATTGCGGCTGTGGTGGAAGTCGGAATGAAGGCCTACCGTTATTTCCGGCCACCGCCCCCTGAGCCATCGGCCGTCTTCGTCTGGAAGTCCATCTGCACCCACTGGGTGGGGGTCCTGGCTGGGCCCGTCATACTGGCGCTGATTTTCAAGCGCACCTACGACATCATCAAGGSizeHit CountTotal NormTotalM021

Embryo		M042

Female-body		V042

Embryo		V050

Head		V057

Head		V111

Male-body
..............GTGGAAGTCGGAATGAAGGC............................................................................................................................................20114.00140000014
..............GTGGAAGTCGGAATGAAGG.............................................................................................................................................19111.00110000011
...........GTGGTGGAAGTCGGAATGAAG..............................................................................................................................................2117.007000007
.............GGTGGAAGTCGGAATGAAGG.............................................................................................................................................2016.006000006
........GCTGTGGTGGAAGTCGGA....................................................................................................................................................1814.004000004
..............GTGGAAGTCGGAATGAAGGCC...........................................................................................................................................2114.004000004
..........................................................................................................GGTGGGGGTCCTGGCTGGG.................................................1953.40170000017
......CGGCTGTGGTGGAAGTCGGA....................................................................................................................................................2013.003000003
.......GGCTGTGGTGGAAGTCGG.....................................................................................................................................................1813.003000003
..........................................................................................................GGTGGGGGTCCTGGCTGG..................................................1852.80140000014
.............GGTGGAAGTCGGAATGAAGGC............................................................................................................................................2112.002000002
.......GGCTGTGGTGGAAGTCGGA....................................................................................................................................................1912.002000002
...........................................................................................................GTGGGGGTCCTGGCTGGG.................................................1851.608000008
........GCTGTGGTGGAAGTCGGAATA.................................................................................................................................................2111.001000001
...............TGGAAGTCGGAATGAAGGC............................................................................................................................................1911.001000001
....................................................................................................................................TACTGGCGCTGATTTTCAAG......................2011.001000001
..............GTGGAAGTCGGAATGAAGGTT...........................................................................................................................................2111.001000001
...TTGCGGCTGTGGTGGAAGTCG......................................................................................................................................................2111.001000001
...........GTGGTGGAAGTCGGAATGAAGG.............................................................................................................................................2211.001000001
..............GTGGAAGTCGGAATGAAGGCCT..........................................................................................................................................2211.001000001
......CGGCTGTGGTGGAAGTCGGAA...................................................................................................................................................2111.001000001
...............TGGAAGTCGGAATGAAGGCC...........................................................................................................................................2011.001000001
.........................................................................................................GGGTGGGGGTCCTGGCTGGG.................................................2050.804000004
...........................................................................................................GTGGGGGTCCTGGCTGGGT................................................1950.402000002
..........................................................................................................GGTGGGGGTCCTGGCTGGGCC...............................................2150.402000002
........................................................................................................TGGGTGGGGGTCCTGGCTG...................................................1950.402000002
........................................................................................................TGGGTGGGGGTCCTGGCTGGG.................................................2150.402000002
..........................................................................................................GGTGGGGGTCCTGGCTGT..................................................1870.292000002
......................................................................................................ACTGGGTGGGGGTCCTGGCTG...................................................2150.201000001
........................................................................................................TGGGTGGGGGTCCTGGCTGG..................................................2050.201000001
......................................................................................................ACTGGGTGGGGGTCCTGGC.....................................................1950.201000001
......................................................................................................ACTGGGTGGGGGTCCTGGCTGGG.................................................2350.201000001
.....................................................................................................CACTGGGTGGGGGTCCTGGC.....................................................2050.201000001
.......................................................................................................CTGGGTGGGGGTCCTGGCTG...................................................2050.201000001
.........................................................................................................GGGTGGGGGTCCTGGCTGG..................................................1950.201000001
.....................................................................................................CACTGGGTGGGGGTCCTGGCTGGG.................................................2450.201000001
...........................................................................................................GTGGGGGTCCTGGCTGGGG................................................1950.201000001
......................................................................................................ACTGGGTGGGGGTCCTGG......................................................1860.171000001

Generated: 10/20/2015 at 10:33 PM