ID:

dps_3244

 Coordinate:

XR_group8:1526246-1526396 -

 Confidence:

Candidate

 Type:

Unknown

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-32.8 -32.8 -32.7

Flybase annnotation

exon [dpse_GLEANR_8092:1]; CDS [Dpse\GA20886-cds]; intron [Dpse\GA20886-in]

No Repeatable elements found

Sense Strand Reads

##################################################---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
ATTGCACTAGTGATAGTTTTTCTCGGCTGCCTTCTCGTGGCACCACTGAGGTGAGTCCTGTGCCCTAAGGAGAAAGTGGAAGAAACTTCGAGTTTGTTCTTTGGTTGCGTGGGGCCGAAGAGGGAGACCTGGAATCGCACGTCCCTCTTGGACTCCAACACCCCCTCAAGGACAAGCACTCAATGTCAATGGTGAACCAGTTTCGATGGAGTCACAATCCTTTGTTGCACACCTGCACACACTGTAGGAGC
**************************************************************.((.(((((.(((...((((...))))...))).))))).)).....(((((((.((((((((....((......))..)))))))))).))))).........************************************************************************************* 		Read size # Mismatch Hit Count Total Norm Total M040
female body
 M062
head
 M022
male body		 M059
embryo
 GSM343916
embryo
................................................................................................................................................CTCTTGGACTCCAACACCCCCT..................................................................................... 22 0 1 2.00 2 2 0 0 0 0
.............................................................................................................................................TCCCTCTTGGACTCCAACACC......................................................................................... 21 0 1 2.00 2 1 0 0 1 0
.............................................................................................................................................TCCCTCTTGGACTCCAACACCC........................................................................................ 22 0 1 2.00 2 1 1 0 0 0
......................TCGGCTGCCTTCTCGTGGCACA............................................................................................................................................................................................................... 22 1 1 1.00 1 0 1 0 0 0
............................................................................................................................................................................................ATGGTGAACCAGTTTCGATGG.......................................... 21 0 1 1.00 1 1 0 0 0 0
.................................................................................................................................................TCTTGGACTCCAACACCCCCTT.................................................................................... 22 1 1 1.00 1 1 0 0 0 0
.........GTGATAGTTTTTCTCGGCTGC............................................................................................................................................................................................................................. 21 0 1 1.00 1 0 0 1 0 0
...............................TTCTCGTGGCACCACTGAGGTGAGT................................................................................................................................................................................................... 25 0 1 1.00 1 1 0 0 0 0
..TGCACTAGTGATAGTTTTTCTCGGC................................................................................................................................................................................................................................ 25 0 1 1.00 1 1 0 0 0 0
..............................................................CCCTAAGGAGAAAGTGGAAGAAACT.................................................................................................................................................................... 25 0 1 1.00 1 1 0 0 0 0
............................................................................................................GTGGGGCCGAAGAGGGAGAC........................................................................................................................... 20 0 1 1.00 1 1 0 0 0 0
......CTAGTGATAGTTTTTCTCGGCT............................................................................................................................................................................................................................... 22 0 1 1.00 1 1 0 0 0 0
.................TTTTCTCGGCTGCCTTCTCGTGGCAC................................................................................................................................................................................................................ 26 0 1 1.00 1 1 0 0 0 0
........AGTGATAGTTTTTCTCGGCTGC............................................................................................................................................................................................................................. 22 0 1 1.00 1 1 0 0 0 0
............................................................................................................................AGACCTGGAATCGCACGTCC........................................................................................................... 20 0 1 1.00 1 1 0 0 0 0
..................................................................................................CTTTGGTTGCGTGGGGCCGA..................................................................................................................................... 20 0 1 1.00 1 1 0 0 0 0
........................................................................................................................................GCACGTCCCTCTTGGACTCC............................................................................................... 20 0 1 1.00 1 1 0 0 0 0
...........................................................................................................................................CGTCCCTCTTGGACTCCAACACA......................................................................................... 23 1 1 1.00 1 1 0 0 0 0
.................................................................................................................................................................CCCCTCAAGGACAAGCACTCA..................................................................... 21 0 1 1.00 1 1 0 0 0 0
..................TTTCTCGGCTGCCTTCTCGTGGCACCA.............................................................................................................................................................................................................. 27 0 1 1.00 1 1 0 0 0 0
.............TAGTTTTTCTCGGCTGCCT........................................................................................................................................................................................................................... 19 0 1 1.00 1 1 0 0 0 0
...................TTCTCGGCTGCCTTCTCGTGGC.................................................................................................................................................................................................................. 22 0 1 1.00 1 1 0 0 0 0
.....................................................................................................................................................................................AATGTGAATGGTGAACCA.................................................... 18 1 2 0.50 1 0 0 0 0 1
...............................................................................................GTTCTTTGATGGCGTGGGG......................................................................................................................................... 19 2 3 0.33 1 1 0 0 0 0
..................................................................................................................................................................................................................GTCACTCCCCTTTGTTGCAC..................... 20 3 13 0.08 1 1 0 0 0 0
.....................................................................................................................................................................................AATCTCGCTGGTGAACCAG................................................... 19 3 18 0.06 1 1 0 0 0 0
...................................................................................................TTTGGTGGCGTGGGGCAG...................................................................................................................................... 18 2 20 0.05 1 1 0 0 0 0

Anti-sense strand reads

TAACGTGATCACTATCAAAAAGAGCCGACGGAAGAGCACCGTGGTGACTCCACTCAGGACACGGGATTCCTCTTTCACCTTCTTTGAAGCTCAAACAAGAAACCAACGCACCCCGGCTTCTCCCTCTGGACCTTAGCGTGCAGGGAGAACCTGAGGTTGTGGGGGAGTTCCTGTTCGTGAGTTACAGTTACCACTTGGTCAAAGCTACCTCAGTGTTAGGAAACAACGTGTGGACGTGTGTGACATCCTCG
*************************************************************************************.((.(((((.(((...((((...))))...))).))))).)).....(((((((.((((((((....((......))..)))))))))).))))).........************************************************************** 		Read size # Mismatch Hit Count Total Norm Total M040
female body
 GSM444067
head
 V112
male body		 M062
head
 M059
embryo
 GSM343916
embryo
 M022
male body
.........................................................................................................................CCCTCTGGACCTAAGCTTG............................................................................................................... 19 2 1 4.00 4 3 1 0 0 0 0 0
...................................................................................................................................................................................................................................GTGTGGACGTGTATGACATCC... 21 1 1 1.00 1 1 0 0 0 0 0 0
..........................................................................................................................................................TGTTGTGGGGGAGTTCCTGT............................................................................. 20 1 1 1.00 1 1 0 0 0 0 0 0
.....................................................................................................................................................................AGTTCCTGTTCGTGAGTTAC.................................................................. 20 0 1 1.00 1 0 0 1 0 0 0 0
......................AGCCGACGGAAGAGCACCGT................................................................................................................................................................................................................. 20 0 1 1.00 1 1 0 0 0 0 0 0
..ACGTGGTCACTATCAAAGAGA.................................................................................................................................................................................................................................... 21 2 1 1.00 1 1 0 0 0 0 0 0
.........................................................................................................................CCCTCTGGACCTAAGCTTGG.............................................................................................................. 20 3 3 0.33 1 0 0 0 1 0 0 0
......................................................................................................................................................................GTTCCTTTTCGGGAGCTACAGT............................................................... 22 3 3 0.33 1 1 0 0 0 0 0 0
.........................CAACGCAGGAGCACCGTGGT.............................................................................................................................................................................................................. 20 3 4 0.25 1 0 0 0 0 1 0 0
........................................................................................................................ACCCTCTGGACCTAAGCTTG............................................................................................................... 20 3 5 0.20 1 0 1 0 0 0 0 0
.............................................................................................................................CTGGACCGTACCGTGCAG............................................................................................................ 18 2 7 0.14 1 0 0 0 0 0 1 0
..........................................................................................................................CCTCTGGACCTAAGCTTGG.............................................................................................................. 19 3 13 0.08 1 1 0 0 0 0 0 0
................................................................................................................................................................GGGGGAGTTGGTGTTCGT......................................................................... 18 2 20 0.05 1 0 0 0 0 0 1 0
...............................................................................................................................................................................................................CCTCAGTGTTAGGCCACG.......................... 18 3 20 0.05 1 0 0 0 0 0 0 1

Re-alignment of all predicted orthologs

Species Coordinate ID Alignment
dp5 XR_group8:1526196-1526446 - dps_3244 ATTGCACTAGTGATAGTTTTTCTCGGCTGCCTTCTCGTGGCA---------CCACTGAGGTGAGTCCTGTGCCCTAAGGAGAAAGTGGAAGAA---ACTTCGAGTTTGTT---CTTTGGTTGCGTGGGGCCGAAGAGGGAGACCTGGAATCGCACGTCCCTCTTGGACTCCAACACCCCC------TCAAGGACAAGCACTCAATGTCAATGGTGAACCAGTTTCGATGGAGTCACAATCCTTTGTTGCACACCTGCACACACTGTAGGAGC
droPer2 scaffold_24:1527514-1527769 + ATTGCACTAGTGATAGTTTTTCTCGGCTGCCTTCTCGTGGCA---------CCACTGAGGTGAGTCCTGTGCCCTAAGGAGAAAGTGGAAGAA---ACTTCGAGTTTGTT----TTTGGTTGCGTGGGGCTGAAGAGGGAGACCTGGAATCGCACGTCCCTCTTGGACTCCAACACCCCCACCTCATCAAGGACAAGCACTCAATGTCAATGGTGAACCAGTTTCGATGGAGTCACAATCCTTTGTCGCACACCTGCACACACTGTAGGACC
droWil2 scf2_1100000004768:2767499-2767501 + AGA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droAna3 scaffold_13337:16054302-16054426 - TTTCTCCTGGTGGCCTCTTTGCTTTGCTGCCTCTTGGCCGCCAGCTCCAGTCCCCTAAAGTAAGTAGC---------GCAGAAATGAAAAGACGCAATTACG---TTGTTTTGTTTTTGTTGCCTTGAAATGGAAAG---------------------------------------------------------------------------------------------------------------------------------------
droBip1 scf7180000396589:379312-379434 + TTTCTCCTGGTGGCCTCTTTGCTTTGCTGCCTCTTGGCTGTCAGCTCCAATCCCCTCAAGTGAGTACC---------GCAGAAACGAAAAGAC---GCAATTACTTTGTT-----TTGTTTTTGTTGGCTTGAAATGGAA------------------------------------------------------------------------------------------------------------------------------------
droKik1 scf7180000302274:301866-301903 + CTTCTAATATGGGCCATTTTGGCCTGCTGCCTTGTGGC------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
dp5
ATTGCACTAGTGATAGTTTTTCTCGGCTGCCTTCTCGTGGCA---------CCACTGAGGTGAGTCCTGTGCCCTAAGGAGAAAGTGGAAGAA---ACTTCGAGTTTGTT---CTTTGGTTGCGTGGGGCCGAAGAGGGAGACCTGGAATCGCACGTCCCTCTTGGACTCCAACACCCCC------TCAAGGACAAGCACTCAATGTCAATGGTGAACCAGTTTCGATGGAGTCACAATCCTTTGTTGCACACCTGCACACACTGTAGGAGCSizeHit CountTotal NormTotalM022
Male-body		M040
Female-body		M059
Embryo		M062
Head		V043
Embryo		V051
Head		V112
Male-body		SRR902010
Ovary		SRR902011
Testis		SRR902012
CNS imaginal disc
...............................................................................................................................................................CTCTTGGACTCCAACACCCCC------T.....................................................................................2212.0020200000000
............................................................................................................................................................TCCCTCTTGGACTCCAACACC...............................................................................................2112.0020110000000
............................................................................................................................................................TCCCTCTTGGACTCCAACACCC..............................................................................................2212.0020101000000
......................TCGGCTGCCTTCTCGTGGCA---------CA...........................................................................................................................................................................................................................2211.0010001000000
.................................................................................................................................................................................................................ATGGTGAACCAGTTTCGATGG..........................................2111.0010100000000
................................................................................................................................................................TCTTGGACTCCAACACCCCC------TT....................................................................................2211.0010100000000
.........GTGATAGTTTTTCTCGGCTGC..................................................................................................................................................................................................................................................2111.0011000000000
...............................TTCTCGTGGCA---------CCACTGAGGTGAGT...............................................................................................................................................................................................................2511.0010100000000
..TGCACTAGTGATAGTTTTTCTCGGC.....................................................................................................................................................................................................................................................2511.0010100000000
.......................................................................CCCTAAGGAGAAAGTGGAAGAA---ACT.............................................................................................................................................................................2511.0010100000000
...........................................................................................................................GTGGGGCCGAAGAGGGAGAC.................................................................................................................................2011.0010100000000
......CTAGTGATAGTTTTTCTCGGCT....................................................................................................................................................................................................................................................2211.0010100000000
.................TTTTCTCGGCTGCCTTCTCGTGGCA---------C............................................................................................................................................................................................................................2611.0010100000000
........AGTGATAGTTTTTCTCGGCTGC..................................................................................................................................................................................................................................................2211.0010100000000
...........................................................................................................................................AGACCTGGAATCGCACGTCC.................................................................................................................2011.0010100000000
.................................................................................................................CTTTGGTTGCGTGGGGCCGA...........................................................................................................................................2011.0010100000000
.......................................................................................................................................................GCACGTCCCTCTTGGACTCC.....................................................................................................2011.0010100000000
..........................................................................................................................................................CGTCCCTCTTGGACTCCAACACA...............................................................................................2311.0010100000000
................................................................................................................................................................................CCCC------TCAAGGACAAGCACTCA.....................................................................2111.0010100000000
..................TTTCTCGGCTGCCTTCTCGTGGCA---------CCA..........................................................................................................................................................................................................................2711.0010100000000
.............TAGTTTTTCTCGGCTGCCT................................................................................................................................................................................................................................................1911.0010100000000
...................TTCTCGGCTGCCTTCTCGTGGC.......................................................................................................................................................................................................................................2211.0010100000000
droPer2
ATTGCACTAGTGATAGTTTTTCTCGGCTGCCTTCTCGTGGCA---------CCACTGAGGTGAGTCCTGTGCCCTAAGGAGAAAGTGGAAGAA---ACTTCGAGTTTGTT----TTTGGTTGCGTGGGGCTGAAGAGGGAGACCTGGAATCGCACGTCCCTCTTGGACTCCAACACCCCCACCTCATCAAGGACAAGCACTCAATGTCAATGGTGAACCAGTTTCGATGGAGTCACAATCCTTTGTCGCACACCTGCACACACTGTAGGACCSizeHit CountTotal NormTotalM021
Embryo		M042
Female-body		V042
Embryo		V050
Head		V057
Head		V111
Male-body
...........................................................................................................................GTGGGGCTGAAGAGGGAGACCTGGA............................................................................................................................2512.002000002
..................TTTCTCGGCTGCCTTCTCGTGGCA---------C............................................................................................................................................................................................................................2511.001100000
...................................................................................AGTGGAAGAA---ACTTCGAGTTT.....................................................................................................................................................................2111.001000001
...GCACTAGTGATAGTTTTTCTCGGCTGCCT................................................................................................................................................................................................................................................2911.001000001
.......TAGTGATAGTTTTTCTCGGCT....................................................................................................................................................................................................................................................2111.001100000
droWil2
AGA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SizeHit CountTotal NormTotalM020
Head		M045
Female-body		V117
Male-body		V118
Embryo		V119
Head
No Reads
droAna3
TTTCTCCTGGTGGCCTCTTTGCTTTGCTGCCTCTTGGCCGCCAGCTCCAGTCCCCTAAAGTAAGTAGC---------GCAGAAATGAAAAGACGCAATTACG---TTGTTTTGTTTTTGTTGCCTTGAAATGGAAAG---------------------------------------------------------------------------------------------------------------------------------------SizeHit CountTotal NormTotalM044
Female-body		M058
Embryo		V039
Embryo		V055
Head		V105
Male-body		V106
Head
No Reads
droBip1
TTTCTCCTGGTGGCCTCTTTGCTTTGCTGCCTCTTGGCTGTCAGCTCCAATCCCCTCAAGTGAGTACC---------GCAGAAACGAAAAGAC---GCAATTACTTTGTT-----TTGTTTTTGTTGGCTTGAAATGGAA------------------------------------------------------------------------------------------------------------------------------------SizeHit CountTotal NormTotal
No Reads
droKik1
CTTCTAATATGGGCCATTTTGGCCTGCTGCCTTGTGGC------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SizeHit CountTotal NormTotal
No Reads

Generated: 05/18/2015 at 06:24 AM