dps_2811

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------################################################## NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCATAGATGTTGATGCGCCCACCACTGATTCATTTAAGGGTTCGAAACCATATCACAGATCTGTCTTAGTGCAGCGTTCCAGTGATCAAGTCGATTTTGCACCACAACAGCAGCAGCAG ************************************************....................................................................................((((((..(((((((...(((..(((.....)))..))).))......)))))...)))))).....************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M040 female body	V112 male body	SRR902011 testis	M059 embryo	GSM444067 head
.............................................................................................................................................................TGATACAATTAAGCGTTCGAA.........................................................................	21	3	6	4.33	26	23	3	0	0	0
...............................................................................................................................................................ATACAATTAAGCGTTCGAAACC......................................................................	22	3	2	3.00	6	5	1	0	0	0
...............................................................................................................................................................ATACAATTAAGCGTTCGAAACCA.....................................................................	23	3	2	2.00	4	4	0	0	0	0
.............................................................................................................................................................TGATACAATTAAGCGTTCGAAA........................................................................	22	3	4	1.00	4	2	2	0	0	0
.......................................................................................................................................TAGATGTTGATGCGCCCACC................................................................................................	20	0	1	1.00	1	0	1	0	0	0
...........................................................................................................................................TGTTGATGCGCCCACCACTG............................................................................................	20	0	1	1.00	1	1	0	0	0	0
..................................................................................................................................................................CAATTAAGCGTTCGAAAC.......................................................................	18	2	3	0.67	2	2	0	0	0	0
........................................................................................................................................................ACCACTGATTCATTTAAGGG...............................................................................	20	0	2	0.50	1	0	1	0	0	0
................................................................................................................................................................TACAATTAAGCGTTCGAAACC......................................................................	21	3	2	0.50	1	1	0	0	0	0
.........................................................................................................................................................CCAATGATTCATTTAAGGGTT.............................................................................	21	1	2	0.50	1	0	1	0	0	0
.........................................................................................................................................................CCACTGATTCATTTAAGGGTT.............................................................................	21	0	2	0.50	1	0	1	0	0	0
.........................................................................................................................................................CCAATGATTCATTTAAGGGTTT............................................................................	22	2	2	0.50	1	0	1	0	0	0
................................................................................................................................................................TACAATTAAGCGTTCGAAACCA.....................................................................	22	3	2	0.50	1	1	0	0	0	0
.......................................................................................................................................TAGATGTTGATACGCGAACC................................................................................................	20	3	3	0.33	1	0	0	0	1	0
...................................................................................................................................GCCATAGATGTTGATGCGCC....................................................................................................	20	0	3	0.33	1	0	0	0	0	1
...................................................................................................................................................CGCCCACCACTGATTCATTT....................................................................................	20	0	3	0.33	1	1	0	0	0	0
....................................................................................................................................CCATAGATGTTGATGCGCCT...................................................................................................	20	1	3	0.33	1	0	1	0	0	0
....................................................................................................................................CCATAGATGTTGATGCGCC....................................................................................................	19	0	3	0.33	1	0	0	1	0	0
..............................................................................................................................................................GATACAATTAAGCGTTCGAAAC.......................................................................	22	3	3	0.33	1	1	0	0	0	0
................................................................................................................................................ATGAGCCCACCACTGATTCA.......................................................................................	20	1	5	0.20	1	0	0	1	0	0
................................................................................................................................................ATGCGCCCACCACTGGTTCAT......................................................................................	21	1	5	0.20	1	0	0	1	0	0
............................................................................................................................................................CTGATACAATTAAGCGTTCGA..........................................................................	21	3	7	0.14	1	1	0	0	0	0
...............................................................................................................................................................ATACAATTAAGTGTTCGAAAC.......................................................................	21	3	8	0.13	1	1	0	0	0	0
.............................................................................................................................................................CGATCCATATAAGGGTTCG...........................................................................	19	3	17	0.06	1	1	0	0	0	0
........................................................................................................................................AGATGTTGATACCACCACC................................................................................................	19	3	18	0.06	1	0	0	0	1	0
.................................................................................................................................................................GCAATTAAGCGTTCGAAAC.......................................................................	19	3	20	0.05	1	1	0	0	0	0

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------##################################################
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCATAGATGTTGATGCGCCCACCACTGATTCATTTAAGGGTTCGAAACCATATCACAGATCTGTCTTAGTGCAGCGTTCCAGTGATCAAGTCGATTTTGCACCACAACAGCAGCAGCAG
**************************************************....................................................................................((((((..(((((((...(((..(((.....)))..))).))......)))))...)))))).....**************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M040
female body

V112
male body

SRR902011
testis

M059
embryo

GSM444067
head

.............................................................................................................................................................TGATACAATTAAGCGTTCGAA.........................................................................

4.33

...............................................................................................................................................................ATACAATTAAGCGTTCGAAACC......................................................................

3.00

...............................................................................................................................................................ATACAATTAAGCGTTCGAAACCA.....................................................................

2.00

.............................................................................................................................................................TGATACAATTAAGCGTTCGAAA........................................................................

1.00

.......................................................................................................................................TAGATGTTGATGCGCCCACC................................................................................................

1.00

...........................................................................................................................................TGTTGATGCGCCCACCACTG............................................................................................

1.00

..................................................................................................................................................................CAATTAAGCGTTCGAAAC.......................................................................

0.67

........................................................................................................................................................ACCACTGATTCATTTAAGGG...............................................................................

0.50

................................................................................................................................................................TACAATTAAGCGTTCGAAACC......................................................................

0.50

.........................................................................................................................................................CCAATGATTCATTTAAGGGTT.............................................................................

0.50

.........................................................................................................................................................CCACTGATTCATTTAAGGGTT.............................................................................

0.50

.........................................................................................................................................................CCAATGATTCATTTAAGGGTTT............................................................................

0.50

................................................................................................................................................................TACAATTAAGCGTTCGAAACCA.....................................................................

0.50

.......................................................................................................................................TAGATGTTGATACGCGAACC................................................................................................

0.33

...................................................................................................................................GCCATAGATGTTGATGCGCC....................................................................................................

0.33

...................................................................................................................................................CGCCCACCACTGATTCATTT....................................................................................

0.33

....................................................................................................................................CCATAGATGTTGATGCGCCT...................................................................................................

0.33

....................................................................................................................................CCATAGATGTTGATGCGCC....................................................................................................

0.33

..............................................................................................................................................................GATACAATTAAGCGTTCGAAAC.......................................................................

0.33

................................................................................................................................................ATGAGCCCACCACTGATTCA.......................................................................................

0.20

................................................................................................................................................ATGCGCCCACCACTGGTTCAT......................................................................................

0.20

............................................................................................................................................................CTGATACAATTAAGCGTTCGA..........................................................................

0.14

...............................................................................................................................................................ATACAATTAAGTGTTCGAAAC.......................................................................

0.13

.............................................................................................................................................................CGATCCATATAAGGGTTCG...........................................................................

0.06

........................................................................................................................................AGATGTTGATACCACCACC................................................................................................

0.06

.................................................................................................................................................................GCAATTAAGCGTTCGAAAC.......................................................................

0.05

NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGGTATCTACAACTACGCGGGTGGTGACTAAGTAAATTCCCAAGCTTTGGTATAGTGTCTAGACAGAATCACGTCGCAAGGTCACTAGTTCAGCTAAAACGTGGTGTTGTCGTCGTCGTC ************************************************....................................................................................((((((..(((((((...(((..(((.....)))..))).))......)))))...)))))).....************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M040 female body	V112 male body	SRR902011 testis	GSM444067 head	M062 head
.............................................................................................................................................................ACTATGTTAATTCGCAAGCTT.........................................................................	21	3	6	8.33	50	46	4	0	0	0
..............................................................................................................................................................CTATGTTAATTCGCAAGCTTTG.......................................................................	22	3	3	2.67	8	8	0	0	0	0
...................................................................................................................................................GCGGGTGGTGACTAAGTAA.....................................................................................	19	0	5	2.40	12	0	8	3	0	1
...................................................................................................................................................GCGGGTGGTGACTAAGTAAAT...................................................................................	21	0	3	2.33	7	0	6	0	1	0
.................................................................................................................................................ACGCGGGTGGTGACTAAGTAA.....................................................................................	21	0	5	1.80	9	0	6	2	1	0
.........................................................................................................................................................GGTGACTAAGTAAATTCCCAA.............................................................................	21	0	2	1.50	3	0	0	3	0	0
.....................................................................................................................................................GGGTGGTGACTAAGTAAAT...................................................................................	19	0	3	1.33	4	0	0	4	0	0
.............................................................................................................................................................ACTATGTTAATTCGCAAGCTTTG.......................................................................	23	3	1	1.00	1	1	0	0	0	0
.............................................................................................................................................................ACTATGTTAATTCGCAAGCTTT........................................................................	22	3	4	0.75	3	3	0	0	0	0
...................................................................................................................................................GCGGGTGGTGACTAAGTAAA....................................................................................	20	0	3	0.67	2	0	2	0	0	0
......................................................................................................................................................GGTGGTGACTAAGTAAAT...................................................................................	18	0	3	0.67	2	0	0	2	0	0
.....................................................................................................................................................GGGTGGTGACTAAGTAAATT..................................................................................	20	0	3	0.67	2	0	0	2	0	0
....................................................................................................................................................CGGGTGGTGACTAAGTAA.....................................................................................	18	0	5	0.60	3	0	0	3	0	0
........................................................................................................................................................TGGTGACTAAGTAAATTCCCAA.............................................................................	22	0	2	0.50	1	1	0	0	0	0
........................................................................................................................................................TGGTGACTAAGTAAATTCC................................................................................	19	0	2	0.50	1	0	0	1	0	0
..........................................................................................................................................................GTGACTAAGTAAATTCCCAA.............................................................................	20	0	2	0.50	1	0	1	0	0	0
..................................................................................................................................................TGCGGGTGGTGACTAAGTAA.....................................................................................	20	1	5	0.40	2	0	2	0	0	0
.....................................................................................................................................................GGGTGGTGACTAAGTAAATTC.................................................................................	21	0	3	0.33	1	0	0	1	0	0
....................................................................................................................................................CGGGTGGTGACTAAGTAAAT...................................................................................	20	0	3	0.33	1	0	0	1	0	0
.................................................................................................................................................TTGCGGGTGGTGACTAAGTAA.....................................................................................	21	2	6	0.33	2	1	1	0	0	0
..................................................................................................................................................CGCGGGTGGTGACTAAGTAAA....................................................................................	21	0	3	0.33	1	0	1	0	0	0
.....................................................................................................................................................TGGTGGTGACTAAGTAAAT...................................................................................	19	1	3	0.33	1	0	1	0	0	0
............................................................................................................................................................GACTAAGTAAATTCCCAAGCT..........................................................................	21	0	4	0.25	1	0	0	1	0	0
............................................................................................................................................................GACTAAGTAAATTCCCAAGC...........................................................................	20	0	4	0.25	1	0	0	1	0	0
...............................................................................................................................................................................................AGACTGAATCAGGTCGCCAG........................................	20	3	4	0.25	1	0	0	0	1	0
............................................................................................................................................................GACTAAGTAAATTCCCAA.............................................................................	18	0	4	0.25	1	0	0	1	0	0
.....................................................................................................................................................GGGTGGTGACTAAGTGAA....................................................................................	18	1	4	0.25	1	1	0	0	0	0
..................................................................................................................................................CGCGGGTGGTGACTAAGTAA.....................................................................................	20	0	5	0.20	1	0	0	1	0	0
....................................................................................................................................................TGGGTGGTGACTAAGTAA.....................................................................................	18	1	5	0.20	1	0	1	0	0	0
.................................................................................................................................................ACGCGGGTGGTGACTAAGTA......................................................................................	20	0	5	0.20	1	0	1	0	0	0
................................................................................................................................................TACGCGGGTGGTGACTAAGT.......................................................................................	20	0	5	0.20	1	0	1	0	0	0
..................................................................................................................................................AGCGGGTGGTGACTAAGTAA.....................................................................................	20	1	5	0.20	1	0	1	0	0	0
................................................................................................................................................TACGCGGGTGGTGACTAAGTAA.....................................................................................	22	0	5	0.20	1	0	1	0	0	0
.................................................................................................................................................ACGCGGGTGGTGACTAAGT.......................................................................................	19	0	5	0.20	1	0	1	0	0	0
..................................................................................................................................................CGGGGGTGGTGACTAAGTAA.....................................................................................	20	1	5	0.20	1	0	0	1	0	0
...............................................................................................................................................ATTTGCGGGTGGTGACTAAGTAA.....................................................................................	23	3	5	0.20	1	0	1	0	0	0
................................................................................................................................................ATTGCGGGTGGTGACTAAGTAA.....................................................................................	22	3	7	0.14	1	0	1	0	0	0
.................................................................................................................................................................CGTTAATTCGCAAGCTTTG.......................................................................	19	3	20	0.05	1	1	0	0	0	0

NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCGGTATCTACAACTACGCGGGTGGTGACTAAGTAAATTCCCAAGCTTTGGTATAGTGTCTAGACAGAATCACGTCGCAAGGTCACTAGTTCAGCTAAAACGTGGTGTTGTCGTCGTCGTC
**************************************************....................................................................................((((((..(((((((...(((..(((.....)))..))).))......)))))...)))))).....**************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M040
female body

V112
male body

SRR902011
testis

GSM444067
head

M062
head

.............................................................................................................................................................ACTATGTTAATTCGCAAGCTT.........................................................................

8.33

..............................................................................................................................................................CTATGTTAATTCGCAAGCTTTG.......................................................................

2.67

...................................................................................................................................................GCGGGTGGTGACTAAGTAA.....................................................................................

2.40

...................................................................................................................................................GCGGGTGGTGACTAAGTAAAT...................................................................................

2.33

.................................................................................................................................................ACGCGGGTGGTGACTAAGTAA.....................................................................................

1.80

.........................................................................................................................................................GGTGACTAAGTAAATTCCCAA.............................................................................

1.50

.....................................................................................................................................................GGGTGGTGACTAAGTAAAT...................................................................................

1.33

.............................................................................................................................................................ACTATGTTAATTCGCAAGCTTTG.......................................................................

1.00

.............................................................................................................................................................ACTATGTTAATTCGCAAGCTTT........................................................................

0.75

...................................................................................................................................................GCGGGTGGTGACTAAGTAAA....................................................................................

0.67

......................................................................................................................................................GGTGGTGACTAAGTAAAT...................................................................................

0.67

.....................................................................................................................................................GGGTGGTGACTAAGTAAATT..................................................................................

0.67

....................................................................................................................................................CGGGTGGTGACTAAGTAA.....................................................................................

0.60

........................................................................................................................................................TGGTGACTAAGTAAATTCCCAA.............................................................................

0.50

........................................................................................................................................................TGGTGACTAAGTAAATTCC................................................................................

0.50

..........................................................................................................................................................GTGACTAAGTAAATTCCCAA.............................................................................

0.50

..................................................................................................................................................TGCGGGTGGTGACTAAGTAA.....................................................................................

0.40

.....................................................................................................................................................GGGTGGTGACTAAGTAAATTC.................................................................................

0.33

....................................................................................................................................................CGGGTGGTGACTAAGTAAAT...................................................................................

0.33

.................................................................................................................................................TTGCGGGTGGTGACTAAGTAA.....................................................................................

0.33

..................................................................................................................................................CGCGGGTGGTGACTAAGTAAA....................................................................................

0.33

.....................................................................................................................................................TGGTGGTGACTAAGTAAAT...................................................................................

0.33

............................................................................................................................................................GACTAAGTAAATTCCCAAGCT..........................................................................

0.25

............................................................................................................................................................GACTAAGTAAATTCCCAAGC...........................................................................

0.25

...............................................................................................................................................................................................AGACTGAATCAGGTCGCCAG........................................

0.25

............................................................................................................................................................GACTAAGTAAATTCCCAA.............................................................................

0.25

.....................................................................................................................................................GGGTGGTGACTAAGTGAA....................................................................................

0.25

..................................................................................................................................................CGCGGGTGGTGACTAAGTAA.....................................................................................

0.20

....................................................................................................................................................TGGGTGGTGACTAAGTAA.....................................................................................

0.20

.................................................................................................................................................ACGCGGGTGGTGACTAAGTA......................................................................................

0.20

................................................................................................................................................TACGCGGGTGGTGACTAAGT.......................................................................................

0.20

..................................................................................................................................................AGCGGGTGGTGACTAAGTAA.....................................................................................

0.20

................................................................................................................................................TACGCGGGTGGTGACTAAGTAA.....................................................................................

0.20

.................................................................................................................................................ACGCGGGTGGTGACTAAGT.......................................................................................

0.20

..................................................................................................................................................CGGGGGTGGTGACTAAGTAA.....................................................................................

0.20

...............................................................................................................................................ATTTGCGGGTGGTGACTAAGTAA.....................................................................................

0.20

................................................................................................................................................ATTGCGGGTGGTGACTAAGTAA.....................................................................................

0.14

.................................................................................................................................................................CGTTAATTCGCAAGCTTTG.......................................................................

0.05

Species	Coordinate	ID	Alignment
dp5	XL_group1e:12254942-12255192 +	dps_2811	NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCATAGATGTTGATGCGCCCACCACTGATTCATTTAAGGGTTCGAAACCATATC-------ACAGATCT------GTCTTAGTGCAGCGTTCCAGTGATCAAGTCGATTT--TGCACCACAACAGCAGCAGCAG
droPer2	scaffold_1:3665601-3665707 -		-----------------------------------------------------------------------------------------------------------------------------------GCCATAGCTGTTCATGCGCCCACCACTGATTCATTTAAGAGTTCGACAACAGATCAGATGCCACAAATCGAGTGCAGTATGAGTGTAGC----------------CGATTTAGTGCGCTACAA------------

Species

Coordinate

Alignment

dp5

XL_group1e:12254942-12255192 +

dps_2811

NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCATAGATGTTGATGCGCCCACCACTGATTCATTTAAGGGTTCGAAACCATATC-------ACAGATCT------GTCTTAGTGCAGCGTTCCAGTGATCAAGTCGATTT--TGCACCACAACAGCAGCAGCAG

droPer2

scaffold_1:3665601-3665707 -

-----------------------------------------------------------------------------------------------------------------------------------GCCATAGCTGTTCATGCGCCCACCACTGATTCATTTAAGAGTTCGACAACAGATCAGATGCCACAAATCGAGTGCAGTATGAGTGTAGC----------------CGATTTAGTGCGCTACAA------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

dp5

NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCATAGATGTTGATGCGCCCACCACTGATTCATTTAAGGGTTCGAAACCATATC-------ACAGATCT------GTCTTAGTGCAGCGTTCCAGTGATCAAGTCGATTT--TGCACCACAACAGCAGCAGCAG	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
.......................................................................................................................................TAGATGTTGATGCGCCCACC...............................................................................................................	20	1	1.00	1	0	0	0	0	0	0	1	0	0	0
...........................................................................................................................................TGTTGATGCGCCCACCACTG...........................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0
........................................................................................................................................................ACCACTGATTCATTTAAGGG..............................................................................................	20	2	0.50	1	0	0	0	0	0	0	1	0	0	0
.........................................................................................................................................................CCACTGATTCATTTAAGGGTT............................................................................................	21	2	0.50	1	0	0	0	0	0	0	1	0	0	0
...................................................................................................................................GCCATAGATGTTGATGCGCC...................................................................................................................	20	3	0.33	1	0	0	0	0	0	1	0	0	0	0
...................................................................................................................................................CGCCCACCACTGATTCATTT...................................................................................................	20	3	0.33	1	0	1	0	0	0	0	0	0	0	0
....................................................................................................................................CCATAGATGTTGATGCGCCT..................................................................................................................	20	3	0.33	1	0	0	0	0	0	0	1	0	0	0
....................................................................................................................................CCATAGATGTTGATGCGCC...................................................................................................................	19	3	0.33	1	0	0	0	0	0	0	0	0	1	0

droPer2

-----------------------------------------------------------------------------------------------------------------------------------GCCATAGCTGTTCATGCGCCCACCACTGATTCATTTAAGAGTTCGACAACAGATCAGATGCCACAAATCGAGTGCAGTATGAGTGTAGC----------------CGATTTAGTGCGCTACAA------------	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
..............................................................................................................................................................................CGACAACAGATCAGATGCCAC.......................................................................	21	1	2.00	2	0	0	0	0	0	2
.........................................................................................................................................................................GAGTTCGACAACAGATCAGATG...........................................................................	22	1	1.00	1	0	0	0	0	0	1
............................................................................................................................................................................TTCGACAACAGATCAGATG...........................................................................	19	1	1.00	1	0	1	0	0	0	0
..............................................................................................................................................................................CGACAACAGATCAGATGCCA........................................................................	20	1	1.00	1	0	0	0	0	0	1

dps_2811

XL_group1e:12254992-12255142 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-8.1	-8.1	-7.4