dps_2042

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------################################################## CGACGACGACGCGGTCGGCAACGCTTCCGACGAAGGCCGTGGATAAGGAGGGGTCGTCAGCCTGCACGAGGGGGAGATCGGCAACTTGGAGCGAGGAGAACAGCGGCCGGCCGCTCTCGGGCCGTTTGTGTCGCAGGAGGTGATCTCTGCGGTGCGAGACGGCATTCTGTGGCACCTTCGAACGCTGGCCCTCCACGCCAGCGCCGCAGGCGATCGAGCCAGAGATAGAGGAGGCGCGGCTGTGGGAGGAG ***********************************************************************************************************************(((((((((((((((((.....)).))))))))).))))))........*******************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M040 female body	SRR902010 ovaries	GSM343916 embryo	M062 head	V112 male body
.....................................................................................................................................CAGGAGGTGATCTCTGCGGTGCGT..............................................................................................	24	1	1	3.00	3	2	1	0	0	0
...................................................................................................................................................TGCGGTGCGAGACGGCATTCTGT.................................................................................	23	0	1	1.00	1	1	0	0	0	0
........................................................TCAGCCTGCACGAGGGGGAGAA.............................................................................................................................................................................	22	1	1	1.00	1	1	0	0	0	0
...................................................................................................................................................TGCGGTGCGTGACGGCATTCTGTGGCACC...........................................................................	29	1	1	1.00	1	0	1	0	0	0
.....................................................................................................................................CAGGAGGTGATCTCTGCGG...................................................................................................	19	0	1	1.00	1	1	0	0	0	0
.................................................................................................................................GTCGCAGGAGGTGATCTCTA......................................................................................................	20	1	1	1.00	1	1	0	0	0	0
.........................................................................................................................CCGTTTGTGTCGCAGGAGG...............................................................................................................	19	0	1	1.00	1	1	0	0	0	0
............................................................CCTGCACGAGGGGGAGATCGGCAACT.....................................................................................................................................................................	26	0	1	1.00	1	1	0	0	0	0
.....................................................................................................................................CAGGAGGTGATCTCTGCGGTGC................................................................................................	22	0	1	1.00	1	1	0	0	0	0
........................TTCCGACGAAGGCCGTGGAT...............................................................................................................................................................................................................	20	0	1	1.00	1	1	0	0	0	0
........................................................TCAGCCTGCACGAGGGGT.................................................................................................................................................................................	18	1	1	1.00	1	1	0	0	0	0
............................................................................................................................................TGATCTCTGCGGTGCGTGACGGCATTC....................................................................................	27	1	1	1.00	1	0	1	0	0	0
.....................................................................................................................................CAGGAGGTGATCTCTGCGGTGCGTG.............................................................................................	25	1	1	1.00	1	1	0	0	0	0
............................................................................................................................................................................................................................AGAGATACAGGAGGGGCGGCC..........	21	3	2	0.50	1	0	0	1	0	0
.......GACGCGGTCCACAACGCT..................................................................................................................................................................................................................................	18	2	2	0.50	1	1	0	0	0	0
..................................................................................................................................TCGCAGGTAGTGATCTCTGGGG...................................................................................................	22	3	2	0.50	1	0	0	1	0	0
..........................................................ACCCTGCCGGAGGGGGAGATC............................................................................................................................................................................	21	3	3	0.33	1	0	0	0	1	0
............................................................CCAGCAGGAGGGGGAGATC............................................................................................................................................................................	19	2	7	0.14	1	1	0	0	0	0
..............................................................................................................................TGTGTCGCAGGAGGTAAA...........................................................................................................	18	2	7	0.14	1	0	0	1	0	0
................................................................................................................................................................................................................................ATAGATGAGGCGGGGCTG.........	18	2	9	0.11	1	1	0	0	0	0
.............................................................................................................................ATGTGTCGCAGGAGGTGA............................................................................................................	18	1	9	0.11	1	1	0	0	0	0
................................................................................................................GTTATCGGGCCGTTTGTGG........................................................................................................................	19	3	19	0.11	2	1	0	0	0	1
.......................................................GTCAGGCTGGACGTGGGGGA................................................................................................................................................................................	20	3	11	0.09	1	0	0	1	0	0
.............................ACGAAGGCCGTGCATAAA............................................................................................................................................................................................................	18	2	13	0.08	1	1	0	0	0	0
......................................................................................................................................................GGTGCGAGAGGTCATTTT...................................................................................	18	3	20	0.05	1	1	0	0	0	0

GCTGCTGCTGCGCCAGCCGTTGCGAAGGCTGCTTCCGGCACCTATTCCTCCCCAGCAGTCGGACGTGCTCCCCCTCTAGCCGTTGAACCTCGCTCCTCTTGTCGCCGGCCGGCGAGAGCCCGGCAAACACAGCGTCCTCCACTAGAGACGCCACGCTCTGCCGTAAGACACCGTGGAAGCTTGCGACCGGGAGGTGCGGTCGCGGCGTCCGCTAGCTCGGTCTCTATCTCCTCCGCGCCGACACCCTCCTC *******************************************************************************(((((((((((((((((.....)).))))))))).))))))........***********************************************************************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M040 female body	GSM343916 embryo	GSM444067 head
....................TGCGAAGGCTGCTTCCGGCACCTAT..............................................................................................................................................................................................................	25	0	1	2.00	2	2	0	0
....................................................................................................................................CGTCCTCCACTAGAGACGCCACGCT..............................................................................................	25	0	1	2.00	2	2	0	0
......................................................................................................................................................................................................GTCGCGGCGTCCGCCAGCTCGGT..............................	23	1	1	1.00	1	1	0	0
....................................................................................................................................................................AAGACACCGTGGAAGCTTGCGACCC..............................................................	25	1	1	1.00	1	1	0	0
...................................................CCAGCAGTCGGACGTGCTCCC...................................................................................................................................................................................	21	0	1	1.00	1	1	0	0
.....................................................................................................................................GTCCTCCACTAGAGACGCCAC.................................................................................................	21	0	1	1.00	1	1	0	0
..................................CCGGCACCTATTCCTCCCCAGCAGT................................................................................................................................................................................................	25	0	1	1.00	1	1	0	0
......................................................................................................................................................................................................GTCTCGGCGTCCGCTAGCTCGGTCT............................	25	1	1	1.00	1	1	0	0
.....................................................................................................................................................................................................................................CCTCCGCGCTGACACCCT....	18	1	12	0.25	3	3	0	0
................................................................................................................................................GAGACGCGACGCTCTGCGCT.......................................................................................	20	3	5	0.20	1	1	0	0
...........................................................................CAAGCCGTTGAACATCACTC............................................................................................................................................................	20	3	5	0.20	1	1	0	0
......................CGAAGGCTTCTTTCGGCATC.................................................................................................................................................................................................................	20	3	7	0.14	1	0	1	0
............................................................................................................................................................................................................................TCTCTATCCCCTCTGTGCCG...........	20	3	12	0.08	1	0	0	1
.....................................................................................................................GCCCGGCAGAGACAGCGTA...................................................................................................................	19	3	20	0.05	1	1	0	0
.............................TGAGTTCGGCACCTATTCCT..........................................................................................................................................................................................................	20	3	20	0.05	1	1	0	0

Species	Coordinate	ID	Alignment
dp5	Unknown_group_489:6529-6779 -	dps_2042	CG----ACGACGACGCGGTCGGCAACGCTTCCGACGAAGGCCGTGGATAAGGAGGGGTCGTCAGCCT--GCACGAGGGGGAG----------ATCGGCAAC-TTGGAGCGAGGAGAACAGCGGCCGGCCGCTCTCGGG-CCGTTTGTGTCGCAGGAGGTGATCTCTGCGGTGCGAGACGGCATTCTGTGGCACCTTCGAACGCT-----------GGCCCTCCACGCCAGCGCCGCAGGCGATCGAGCCAGAGATAGAGGAGGCGCGGCTGTGGGAGGAG
droPer2	scaffold_6911:666-914 -		CGCCGGACGACAACACGCGCGGCCAA--------------------------GGGGATCAGCAGCCGCGGCAGCAGCAGCAGTATCAGCAGCATCAGCAGCACTGGCACGGG-----CCACAGGGAGCCGCCATTGGGCCCATTCGTCTCGCAAGAGGTGCGCTCCGCGGTAAGAGACGGCATGTTGTGGCACGTGCAAACGCTGCACATATCGTGGGCCTCGGGGCCCGCGCCTCAGCAGCCCGAGCAGGAGCCGGGGGAGGCACGACTGTGGGAAGAG

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

dp5

CG----ACGACGACGCGGTCGGCAACGCTTCCGACGAAGGCCGTGGATAAGGAGGGGTCGTCAGCCT--GCACGAGGGGGAG----------ATCGGCAAC-TTGGAGCGAGGAGAACAGCGGCCGGCCGCTCTCGGG-CCGTTTGTGTCGCAGGAGGTGATCTCTGCGGTGCGAGACGGCATTCTGTGGCACCTTCGAACGCT-----------GGCCCTCCACGCCAGCGCCGCAGGCGATCGAGCCAGAGATAGAGGAGGCGCGGCTGTGGGAGGAG	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
.......................................................................................................................................................CAGGAGGTGATCTCTGCGGTGCGT.........................................................................................................	24	1	3.00	3	0	2	0	0	0	0	0	1	0	0
.....................................................................................................................................................................TGCGGTGCGAGACGGCATTCTGT............................................................................................	23	1	1.00	1	0	1	0	0	0	0	0	0	0	0
............................................................TCAGCCT--GCACGAGGGGGAG----------AA..........................................................................................................................................................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................................................CAGGAGGTGATCTCTGCGG..............................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...................................................................................................................................................GTCGCAGGAGGTGATCTCTA.................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...........................................................................................................................................CCGTTTGTGTCGCAGGAGG..........................................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0
................................................................CCT--GCACGAGGGGGAG----------ATCGGCAAC-T.................................................................................................................................................................................	26	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................................................CAGGAGGTGATCTCTGCGGTGC...........................................................................................................	22	1	1.00	1	0	1	0	0	0	0	0	0	0	0
............................TTCCGACGAAGGCCGTGGAT........................................................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0
............................................................TCAGCCT--GCACGAGGGGT........................................................................................................................................................................................................	18	1	1.00	1	0	1	0	0	0	0	0	0	0	0

droPer2

CGCCGGACGACAACACGCGCGGCCAA--------------------------GGGGATCAGCAGCCGCGGCAGCAGCAGCAGTATCAGCAGCATCAGCAGCACTGGCACGGG-----CCACAGGGAGCCGCCATTGGGCCCATTCGTCTCGCAAGAGGTGCGCTCCGCGGTAAGAGACGGCATGTTGTGGCACGTGCAAACGCTGCACATATCGTGGGCCTCGGGGCCCGCGCCTCAGCAGCCCGAGCAGGAGCCGGGGGAGGCACGACTGTGGGAAGAG	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
...................................................................................................................................................................TCCGCGGTAAGAGACGGCATGTTG.............................................................................................	24	3	6.67	20	0	20	0	0	0	0
..........................................................................................................................................................................................................................................TCAGCAGCCCGAGCAGGAGCCGG.......................	23	1	3.00	3	0	2	1	0	0	0
...............................................................................................................G-----CCACAGGGAGCCGCCATTGGG..............................................................................................................................................	22	7	2.86	20	0	18	2	0	0	0
...................................................................................................................................................................TCCGCGGTAAGAGACGGCATGTT..............................................................................................	23	3	2.00	6	0	6	0	0	0	0
...................................................................................................................................................................TCCGCGGTAAGAGACGGCATGT...............................................................................................	22	3	1.67	5	0	5	0	0	0	0
...............................................................................................................G-----CCACAGGGAGCCGCCATTGG...............................................................................................................................................	21	7	1.57	11	0	11	0	0	0	0
...................................................................................................................................................................TCCGCGGTAAGAGACGGCATGTTGTGG..........................................................................................	27	3	1.33	4	0	4	0	0	0	0
...............................................................................................................G-----CCACAGGGAGCCGCCATTGGGC.............................................................................................................................................	23	7	1.14	8	0	8	0	0	0	0
..........................................................................................................................................................................................................................................TCAGCAGCCCGAGCAGGAGC..........................	20	1	1.00	1	0	1	0	0	0	0
..................................................................................................................................................................................................................................................CCGAGCAGGAGCCGGGGGAGG.................	21	1	1.00	1	0	1	0	0	0	0
...................................................................................................................................................................TCCGCGGTAAGAGACGGCATGTTGT............................................................................................	25	3	1.00	3	0	3	0	0	0	0
...................................................................................................................................................................TCCGCGGTAAGAGACGGCATG................................................................................................	21	3	1.00	3	0	3	0	0	0	0
...............................................................................................................G-----CCACAGGGAGCCGCCATTGGGCC............................................................................................................................................	24	7	0.86	6	0	6	0	0	0	0
....................................................................................................................................................................CCGCGGTAAGAGACGGCATGTTG.............................................................................................	23	3	0.67	2	0	2	0	0	0	0
....................................................................................................................................................................CCGCGGTAAGAGACGGCATG................................................................................................	20	3	0.67	2	0	2	0	0	0	0
..........................................................................................................................................................GAGGTGCGCTCCGCGGTAAGAGA.......................................................................................................	23	3	0.67	2	0	2	0	0	0	0
..........................................................................................................................................................GAGGTGCGCTCCGCGGTAAGAGACGG....................................................................................................	26	3	0.67	2	0	2	0	0	0	0
..................................................................................................................................................................................................TGCAAACGCTGCACATATCGTGGGCC............................................................	26	16	0.56	9	0	9	0	0	0	0
.....................................................................................................................CCACAGGGAGCCGCCATTGGG..............................................................................................................................................	21	7	0.43	3	0	3	0	0	0	0
....................................................................................................................................................................CCGCGGTAAGAGACGGCATGTTGT............................................................................................	24	3	0.33	1	0	1	0	0	0	0
..........................................................................................................................................................GAGGTGCGCTCCGCGGTAAGAGACG.....................................................................................................	25	3	0.33	1	0	1	0	0	0	0
..........................................................................................................................................................GAGGTGCGCTCCGCGGTAAGA.........................................................................................................	21	3	0.33	1	0	1	0	0	0	0
....................................................................................................................................................................CCGCGGTAAGAGACGGCATGTTGTG...........................................................................................	25	3	0.33	1	0	1	0	0	0	0
....................................................GGGGATCAGCAGCCGCGGCAG...............................................................................................................................................................................................................	21	3	0.33	1	0	1	0	0	0	0
.....................................................................................................................................................................CGCGGTAAGAGACGGCATGTTG.............................................................................................	22	3	0.33	1	0	1	0	0	0	0
.....................................................GGGATCAGCAGCCGCGGCAGCAG............................................................................................................................................................................................................	23	3	0.33	1	0	1	0	0	0	0
..............................................................................................................................................................................AGACGGCATGTTGTGGCA........................................................................................	18	5	0.20	1	0	1	0	0	0	0
..................................................................................................................................................................................................TGCAAACGCTGCACATATCGTGGGCCT...........................................................	27	16	0.19	3	0	3	0	0	0	0
.........................................................................................................................................................................................................GCTGCACATATCGTGGGCCTCGG........................................................	23	6	0.17	1	0	1	0	0	0	0
.........................................................................................................................................................................................................GCTGCACATATCGTGGGC.............................................................	18	18	0.17	3	3	0	0	0	0	0
.........................................................................................................................................................................................................GCTGCACATATCGTGGGCCTCGGGG......................................................	25	6	0.17	1	0	1	0	0	0	0
.................................................................................................................................................................................................................TATCGTGGGCCTCGGGGCCC...................................................	20	6	0.17	1	0	1	0	0	0	0
.........................................................................................................................................................................................................GCTGCACATATCGTGGGCCTCGT........................................................	23	6	0.17	1	0	1	0	0	0	0
..........................................................................................................CACGGG-----CCACAGGGAGCCGCCATTG................................................................................................................................................	25	7	0.14	1	0	1	0	0	0	0
.............................................................CAGCCGCGGCAGCAGCAGCA.......................................................................................................................................................................................................	20	7	0.14	1	0	1	0	0	0	0
...............................................................................................................G-----CCACAGGGAGCCGCCATTG................................................................................................................................................	20	7	0.14	1	0	1	0	0	0	0
........................................................................................................................CAGGGAGCCGCCATTGGGC.............................................................................................................................................	19	8	0.13	1	0	1	0	0	0	0
.......................................................................................................TGGCACGGG-----CCACAGGGAGCCGCCATT.................................................................................................................................................	27	8	0.13	1	0	1	0	0	0	0
..................................................................................................................................................................................................TGCAAACGCTGCACATATCGTGGGC.............................................................	25	16	0.13	2	0	2	0	0	0	0
.......................................................................................................TGGCACGGG-----CCACAGGGAGCCGC.....................................................................................................................................................	23	14	0.07	1	0	1	0	0	0	0
.......................................................................................................TGGCACGGG-----CCACAGGGAGCCGCCA...................................................................................................................................................	25	14	0.07	1	0	1	0	0	0	0
...............................................................................................AGCAGCACTGGCACGGG-----CCACAGG............................................................................................................................................................	24	14	0.07	1	0	1	0	0	0	0
.......................................................................................................TGGCACGGG-----CCACAGGGAGC........................................................................................................................................................	20	15	0.07	1	0	1	0	0	0	0
........................................................................................................GGCACGGG-----CCACAGGGAGA........................................................................................................................................................	19	15	0.07	1	0	1	0	0	0	0
...................................................................................................................................................................................................GCAAACGCTGCACATATCGTGGGCCT...........................................................	26	16	0.06	1	0	1	0	0	0	0
..................................................................................................................................................................................................TGCAAACGCTGCACATATCGTGGGCCTC..........................................................	28	16	0.06	1	0	1	0	0	0	0
...................................................................................................................................................................................................GCAAACGCTGCACATATCGTGGG..............................................................	23	16	0.06	1	0	1	0	0	0	0
.........................................................................................................................................................................................................GCTGCACATATCGTGGGCCTC..........................................................	21	18	0.06	1	0	1	0	0	0	0

dps_2042

Unknown_group_489:6579-6729 -

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-18.4	-18.3	-18.3