dps_1927

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------################################################## TTGATTGTTCCCATTTCATATGGGTGAGAATGAAGGTTTGACATTTAGTCACAAAATTCTACACGTCAATTTTGGATATTGGAGGATATTTTGTTTTGTTTTAGATCTTTTTAGCGCAGGTCCGTGTATCGATTTTTTACCATCAATTCTTCCTCTCTTCACGAATCTTTAGCTGCTGGTGCGTCTGCGGACGGAGAAGAGGCTACCCACCCCCCAAATGGAGGTCCTGCCGCCTCTGGCTTGCAGAAGGA *************************************************************.......((((((....((((((....(((....((...(((((......((((......))))...)))))...))...)))....))))))..)).))))...............*********************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR902011 testis	M040 female body	SRR902010 ovaries	V112 male body	M059 embryo	GSM343916 embryo
.............................................................................................................................................................................................GACGGAGAAGAGGCTACCCA..........................................	20	0	3	0.67	2	2	0	0	0	0	0
.............................................................................................................................................................................................GACGGAGAAGAGGCTACCCAC.........................................	21	0	3	0.67	2	2	0	0	0	0	0
.............................................................................................................................................................................................GACGGAGAAGAGGCTACCCACC........................................	22	0	3	0.33	1	1	0	0	0	0	0
...............................................................CGTCAATTTTGGATATTGGAGG......................................................................................................................................................................	22	0	3	0.33	1	1	0	0	0	0	0
..................................................................CAATTTTGGATATTGGAGGAT....................................................................................................................................................................	21	0	3	0.33	1	0	1	0	0	0	0
.........................................................................................................................................................................................TGCGGACGGAGAAGAGGCTAACCA..........................................	24	1	3	0.33	1	1	0	0	0	0	0
.......................................................................................................................................................................................TCTGCGGACGGAGAAGAGGC................................................	20	0	3	0.33	1	0	0	1	0	0	0
..........................................................................................................................................................................................GCGGACGGAGAAGAGGCTACCCACC........................................	25	0	3	0.33	1	1	0	0	0	0	0
...............................................................................................................................................................CACGAATCTTTAGCTGCTGGT.......................................................................	21	0	3	0.33	1	0	1	0	0	0	0
............................................................................................................................................................................................................................GAGGTCCTGCCGCCTCTGGCTTGCAGAAG..	29	0	6	0.33	2	2	0	0	0	0	0
.....................................................................TTTTGGATATTGGAGGATATT.................................................................................................................................................................	21	0	3	0.33	1	1	0	0	0	0	0
...........................................................................................................................................................................................CGGACGGAGAAGAGGCTACCCAC.........................................	23	0	3	0.33	1	1	0	0	0	0	0
........................................................TTCTACACGTCAATTTTGGAT..............................................................................................................................................................................	21	0	3	0.33	1	1	0	0	0	0	0
...................................................................................................................................................................................................................................TGCCGCCTCTGGCTTGCAGAAGGA	24	0	3	0.33	1	1	0	0	0	0	0
...........................................................................................................TTTTTAGCGCAGGTCCGTGTA...........................................................................................................................	21	0	4	0.25	1	0	1	0	0	0	0
...................................................CAAAATTCTACACGTCAATTT...................................................................................................................................................................................	21	0	4	0.25	1	0	0	0	1	0	0
.....................................................................................................TAGATCTTTTTAGCGCAGGTC.................................................................................................................................	21	0	4	0.25	1	0	1	0	0	0	0
..........................................................................GATATTGGTGGGTATTTTGT.............................................................................................................................................................	20	2	5	0.20	1	0	1	0	0	0	0
.....................................................................................................................................................................................................AGAGGCTACACACCCACCA...................................	19	2	5	0.20	1	0	1	0	0	0	0
.................................................................................................................................................................................................................................CCTGCCGCCTCTGGCTTGCA......	20	0	6	0.17	1	0	1	0	0	0	0
....................................................................................................................................................................................................AAGAGGCTACACCCCCCAC....................................	19	3	20	0.10	2	0	0	0	0	1	1
...................ATGGGTGAGGATTAAGCT......................................................................................................................................................................................................................	18	3	20	0.10	2	0	2	0	0	0	0
.......................................................................................................GATCTTTTTAACGCAGC...................................................................................................................................	17	2	20	0.10	2	2	0	0	0	0	0
.........................................................................................................................................................................TAGCTGCGGGTGCGTCCTC...............................................................	19	3	20	0.05	1	0	1	0	0	0	0

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------##################################################
TTGATTGTTCCCATTTCATATGGGTGAGAATGAAGGTTTGACATTTAGTCACAAAATTCTACACGTCAATTTTGGATATTGGAGGATATTTTGTTTTGTTTTAGATCTTTTTAGCGCAGGTCCGTGTATCGATTTTTTACCATCAATTCTTCCTCTCTTCACGAATCTTTAGCTGCTGGTGCGTCTGCGGACGGAGAAGAGGCTACCCACCCCCCAAATGGAGGTCCTGCCGCCTCTGGCTTGCAGAAGGA
***************************************************************.......((((((....((((((....(((....((...(((((......((((......))))...)))))...))...)))....))))))..)).))))...............***********************************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

SRR902011
testis

M040
female body

SRR902010
ovaries

V112
male body

M059
embryo

GSM343916
embryo

.............................................................................................................................................................................................GACGGAGAAGAGGCTACCCA..........................................

0.67

0.33

...............................................................CGTCAATTTTGGATATTGGAGG......................................................................................................................................................................

0.33

..................................................................CAATTTTGGATATTGGAGGAT....................................................................................................................................................................

0.33

.........................................................................................................................................................................................TGCGGACGGAGAAGAGGCTAACCA..........................................

0.33

.......................................................................................................................................................................................TCTGCGGACGGAGAAGAGGC................................................

0.33

..........................................................................................................................................................................................GCGGACGGAGAAGAGGCTACCCACC........................................

0.33

...............................................................................................................................................................CACGAATCTTTAGCTGCTGGT.......................................................................

0.33

............................................................................................................................................................................................................................GAGGTCCTGCCGCCTCTGGCTTGCAGAAG..

0.33

.....................................................................TTTTGGATATTGGAGGATATT.................................................................................................................................................................

0.33

...........................................................................................................................................................................................CGGACGGAGAAGAGGCTACCCAC.........................................

0.33

........................................................TTCTACACGTCAATTTTGGAT..............................................................................................................................................................................

0.33

...................................................................................................................................................................................................................................TGCCGCCTCTGGCTTGCAGAAGGA

0.33

...........................................................................................................TTTTTAGCGCAGGTCCGTGTA...........................................................................................................................

0.25

...................................................CAAAATTCTACACGTCAATTT...................................................................................................................................................................................

0.25

.....................................................................................................TAGATCTTTTTAGCGCAGGTC.................................................................................................................................

0.25

..........................................................................GATATTGGTGGGTATTTTGT.............................................................................................................................................................

0.20

.....................................................................................................................................................................................................AGAGGCTACACACCCACCA...................................

0.20

.................................................................................................................................................................................................................................CCTGCCGCCTCTGGCTTGCA......

0.17

....................................................................................................................................................................................................AAGAGGCTACACCCCCCAC....................................

0.10

...................ATGGGTGAGGATTAAGCT......................................................................................................................................................................................................................

0.10

.......................................................................................................GATCTTTTTAACGCAGC...................................................................................................................................

0.10

.........................................................................................................................................................................TAGCTGCGGGTGCGTCCTC...............................................................

0.05

AACTAACAAGGGTAAAGTATACCCACTCTTACTTCCAAACTGTAAATCAGTGTTTTAAGATGTGCAGTTAAAACCTATAACCTCCTATAAAACAAAACAAAATCTAGAAAAATCGCGTCCAGGCACATAGCTAAAAAATGGTAGTTAAGAAGGAGAGAAGTGCTTAGAAATCGACGACCACGCAGACGCCTGCCTCTTCTCCGATGGGTGGGGGGTTTACCTCCAGGACGGCGGAGACCGAACGTCTTCCT *********************************************************************.......((((((....((((((....(((....((...(((((......((((......))))...)))))...))...)))....))))))..)).))))...............*************************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M040 female body	V112 male body
.............................................ATCAGTGTTTTAAGATGTGCA.........................................................................................................................................................................................	21	0	4	0.25	1	1	0
..............................................TCAGCGGGTTAAGATGTGCAGT.......................................................................................................................................................................................	22	3	4	0.25	1	1	0
................................................................................................................................................TCAAGAATGAGAGAAGTG.........................................................................................	18	2	5	0.20	1	1	0
.............AAAGTATACCGGCTCTTACGT.........................................................................................................................................................................................................................	21	3	6	0.17	1	1	0
..................................................................................................................GCGTCCAGGCACATAGCTAA.....................................................................................................................	20	0	7	0.14	1	0	1
..................................................................................................................................CTTCAGAATGGTAGTTAAG......................................................................................................	19	3	20	0.05	1	1	0

AACTAACAAGGGTAAAGTATACCCACTCTTACTTCCAAACTGTAAATCAGTGTTTTAAGATGTGCAGTTAAAACCTATAACCTCCTATAAAACAAAACAAAATCTAGAAAAATCGCGTCCAGGCACATAGCTAAAAAATGGTAGTTAAGAAGGAGAGAAGTGCTTAGAAATCGACGACCACGCAGACGCCTGCCTCTTCTCCGATGGGTGGGGGGTTTACCTCCAGGACGGCGGAGACCGAACGTCTTCCT
***********************************************************************.......((((((....((((((....(((....((...(((((......((((......))))...)))))...))...)))....))))))..)).))))...............***************************************************************

Read size

# Mismatch

Hit Count

Total Norm

Total

M040
female body

V112
male body

.............................................ATCAGTGTTTTAAGATGTGCA.........................................................................................................................................................................................

0.25

..............................................TCAGCGGGTTAAGATGTGCAGT.......................................................................................................................................................................................

0.25

................................................................................................................................................TCAAGAATGAGAGAAGTG.........................................................................................

0.20

.............AAAGTATACCGGCTCTTACGT.........................................................................................................................................................................................................................

0.17

..................................................................................................................GCGTCCAGGCACATAGCTAA.....................................................................................................................

0.14

..................................................................................................................................CTTCAGAATGGTAGTTAAG......................................................................................................

0.05

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
dp5	Unknown_group_265:20537-20787 +	dps_1927	TTGATTGTTCCCATTTCATATGGGTGAGAATGAAGGTTTGACATTTAGTCACAAAATTCTACACGTCAATTTTGGATATTGGAGGATATTTTGTTTTGTTTTAGATCTTTTTAGCGCAGGTCCGTGTATCGATTTTTTACCATCAATTCTTCCTCTCTTCACGAATCTTTAGCTGCTGGTGCGTCTGCGGACGGAGAAGAGGCTACCCACCCCCCAAAT--GGAGGTCCTGCCGCCTCTGGCTTGCAGAAGGA
droPer2	scaffold_77:48376-48626 -		TTGATTGTTCCCATTTCATATGGGTAAGAATGAAGGTTTGACATTTAGTCACAAAATTCTGCACGTCAATTTTGGATATTGAAGGATATTTCGTTTTGTTTTAGATCTTTTTAGCGCAGGTCCGTGTATCGATTTTTTACCATCAATTCTTCCTCTCTTCACGAATCTTCAGCTGCTGGTGCGTCTGCGGACGAAGAAGAGGCTACCCACCCCCCAAAT--GGAGGTCCTGCCTCCTCTGGCTTGCAGAAGGA
droBip1	scf7180000396709:230403-230408 +		-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GGAGAA
droEle1	scf7180000491000:943547-943607 +		----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ACAGGCGGGAGGCCAGCCATTCACCAGCTCCGGAGCATCTACCTCTGGTGGCCAACA--GGGC
droEug1	scf7180000408844:281595-281602 +		-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAACAGCA

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

dp5

TTGATTGTTCCCATTTCATATGGGTGAGAATGAAGGTTTGACATTTAGTCACAAAATTCTACACGTCAATTTTGGATATTGGAGGATATTTTGTTTTGTTTTAGATCTTTTTAGCGCAGGTCCGTGTATCGATTTTTTACCATCAATTCTTCCTCTCTTCACGAATCTTTAGCTGCTGGTGCGTCTGCGGACGGAGAAGAGGCTACCCACCCCCCAAAT--GGAGGTCCTGCCGCCTCTGGCTTGCAGAAGGA	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
.............................................................................................................................................................................................GACGGAGAAGAGGCTACCCA............................................	20	3	0.67	2	0	0	0	0	0	0	0	0	2	0
.............................................................................................................................................................................................GACGGAGAAGAGGCTACCCAC...........................................	21	3	0.67	2	0	0	0	0	0	0	0	0	2	0
.............................................................................................................................................................................................GACGGAGAAGAGGCTACCCACC..........................................	22	3	0.33	1	0	0	0	0	0	0	0	0	1	0
...............................................................CGTCAATTTTGGATATTGGAGG........................................................................................................................................................................	22	3	0.33	1	0	0	0	0	0	0	0	0	1	0
..................................................................CAATTTTGGATATTGGAGGAT......................................................................................................................................................................	21	3	0.33	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................................................................................................................TCTGCGGACGGAGAAGAGGC..................................................	20	3	0.33	1	0	0	0	0	0	0	0	1	0	0
..........................................................................................................................................................................................GCGGACGGAGAAGAGGCTACCCACC..........................................	25	3	0.33	1	0	0	0	0	0	0	0	0	1	0
...............................................................................................................................................................CACGAATCTTTAGCTGCTGGT.........................................................................	21	3	0.33	1	0	1	0	0	0	0	0	0	0	0
..............................................................................................................................................................................................................................GAGGTCCTGCCGCCTCTGGCTTGCAGAAG..	29	6	0.33	2	0	0	0	0	0	0	0	0	2	0
.....................................................................TTTTGGATATTGGAGGATATT...................................................................................................................................................................	21	3	0.33	1	0	0	0	0	0	0	0	0	1	0
...........................................................................................................................................................................................CGGACGGAGAAGAGGCTACCCAC...........................................	23	3	0.33	1	0	0	0	0	0	0	0	0	1	0
........................................................TTCTACACGTCAATTTTGGAT................................................................................................................................................................................	21	3	0.33	1	0	0	0	0	0	0	0	0	1	0
.....................................................................................................................................................................................................................................TGCCGCCTCTGGCTTGCAGAAGGA	24	3	0.33	1	0	0	0	0	0	0	0	0	1	0
...........................................................................................................TTTTTAGCGCAGGTCCGTGTA.............................................................................................................................	21	4	0.25	1	0	1	0	0	0	0	0	0	0	0
...................................................CAAAATTCTACACGTCAATTT.....................................................................................................................................................................................	21	4	0.25	1	0	0	0	0	0	0	1	0	0	0
.....................................................................................................TAGATCTTTTTAGCGCAGGTC...................................................................................................................................	21	4	0.25	1	0	1	0	0	0	0	0	0	0	0
...................................................................................................................................................................................................................................CCTGCCGCCTCTGGCTTGCA......	20	6	0.17	1	0	1	0	0	0	0	0	0	0	0

droPer2

TTGATTGTTCCCATTTCATATGGGTAAGAATGAAGGTTTGACATTTAGTCACAAAATTCTGCACGTCAATTTTGGATATTGAAGGATATTTCGTTTTGTTTTAGATCTTTTTAGCGCAGGTCCGTGTATCGATTTTTTACCATCAATTCTTCCTCTCTTCACGAATCTTCAGCTGCTGGTGCGTCTGCGGACGAAGAAGAGGCTACCCACCCCCCAAAT--GGAGGTCCTGCCTCCTCTGGCTTGCAGAAGGA	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
....................................................................................................................................................CTTCCTCTCTTCACGAATCTTCAGC................................................................................	25	2	0.50	1	0	1	0	0	0	0
...................................................................................................................GCAGGTCCGTGTATCGATT.......................................................................................................................	19	5	0.20	1	0	0	0	0	0	1

droBip1

-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GGAGAA	Size	Hit Count	Total Norm	Total
No Reads

droEle1

----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ACAGGCGGGAGGCCAGCCATTCACCAGCTCCGGAGCATCTACCTCTGGTGGCCAACA--GGGC	Size	Hit Count	Total Norm	Total
No Reads

droEug1

-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAACAGCA	Size	Hit Count	Total Norm	Total
No Reads

dps_1927

Unknown_group_265:20587-20737 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-14.9	-14.5	-14.4