dps_1204

intron [Dpse\GA24967-in]; CDS [Dpse\GA24967-cds]; exon [dpse_GLEANR_16041:7]; exon [dpse_GLEANR_16041:6]; CDS [Dpse\GA24967-cds]; intron [Dpse\GA24967-in]

Sense Strand Reads

Anti-sense strand reads

##################################################------------------------------------------------------------------################################################-- CCTGAACCATCCGAAGAGCAGTCTGAAAACATTCCGCCTAAGAAGACAACGTGGGTATATTTGCTTATAATGCAAGATTTTCATTTGAACAGGCATTATCTTTATTGTAATTTCAGAAAGCCGTCTTCTTTGAGCTCCGCTCGTGATGACGTAGTAACTATTGGGT ************************************************.........((...((((((((.(((((...................)))))))))))))....))************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M040 female body	V112 male body	M059 embryo	GSM343916 embryo
.................................................................................CATTTGAAAAGGCAGTATCTA................................................................	21	3	4	35.50	142	112	30	0	0
.................................................................................CATTTGAAAAGGCAGTATCT.................................................................	20	2	1	32.00	32	27	5	0	0
..................................................................................ATTTGAAAAGGCAGTATCT.................................................................	19	2	1	25.00	25	24	1	0	0
................................................................................CCATTTGAAAAGGCAGTATCT.................................................................	21	3	2	6.50	13	10	3	0	0
.................................................................................CATTTGAAAAGGCAGTATC..................................................................	19	2	2	4.00	8	7	1	0	0
..................................................................................ATTTGAAAAGGCAGTATCTA................................................................	20	3	8	3.38	27	27	0	0	0
.................................................................................CATTTGAAAAGGCAGTATCTT................................................................	21	2	1	2.00	2	2	0	0	0
.................................................................................CATTTGAAAAGGCAGTATCTTTT..............................................................	23	3	1	1.00	1	0	1	0	0
................................TCCGCCTAAGAAGACAACAAAG................................................................................................................	22	3	1	1.00	1	0	0	1	0
....................................................................................................................................AGCTCCGCTCGTGATGACGTAG............	22	0	1	1.00	1	0	0	0	1
................................................................................CCATTTGAAAAGGCAGTATC..................................................................	20	3	7	0.86	6	4	2	0	0
..................................................................................ATTTGAAAAGGCAGTATC..................................................................	18	2	4	0.50	2	2	0	0	0
...................................................................................TTTGAAAAGGCAGTATCT.................................................................	18	2	8	0.38	3	3	0	0	0
.................................................................................CATTTGAAAAGGCAGTAT...................................................................	18	2	9	0.33	3	3	0	0	0
................................................................................GCATTTGAAAAGGCAGTATCT.................................................................	21	3	3	0.33	1	1	0	0	0
.................................................................................CATTTGAAGAGGCAGTATCTA................................................................	21	3	4	0.25	1	1	0	0	0
..................................................................................ATTTGAAAAGGCATTATCTAG...............................................................	21	3	4	0.25	1	1	0	0	0
...................................................................................TTTGAAAAGGCATTATCTAGA..............................................................	21	3	5	0.20	1	1	0	0	0
.................................................................................CATTTGAAAAGGCAGTATCA.................................................................	20	3	8	0.13	1	1	0	0	0
..................................................................................ATTTGAAAAGGCAGTATCTG................................................................	20	3	9	0.11	1	1	0	0	0

GGACTTGGTAGGCTTCTCGTCAGACTTTTGTAAGGCGGATTCTTCTGTTGCACCCATATAAACGAATATTACGTTCTAAAAGTAAACTTGTCCGTAATAGAAATAACATTAAAGTCTTTCGGCAGAAGAAACTCGAGGCGAGCACTACTGCATCATTGATAACCCA ************************************************.........((...((((((((.(((((...................)))))))))))))....))************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M040 female body	V112 male body
................................................................................GGTAAACTTTTCCGTCATAGA.................................................................	21	3	2	27.00	54	54	0
.................................................................................GTAAACTTTTCCGTCATAGAT................................................................	21	3	4	15.00	60	59	1
.................................................................................GTAAACTTTTCCGTCATAGA.................................................................	20	2	1	3.00	3	3	0
..................................................................................TAAACTTTTCCGTCATAGA.................................................................	19	2	1	1.00	1	1	0
................................................................................GGTAAACTTTTCCGTCATAG..................................................................	20	3	7	0.86	6	6	0
..................................................................................TAAACTTTTCCGTCATAGAT................................................................	20	3	8	0.38	3	3	0
.................................................................TTATTAGGTTCTGAAAGTAAA................................................................................	21	3	16	0.06	1	1	0

Re-alignment of all predicted orthologs

Species	Coordinate	ID	Alignment
dp5	3:13541924-13542089 +	dps_1204	CCTGAACCATCCGAAGAGCAGTCTGAAAACATTCCGCCTAAGAAGACAACGTGGGTATATTTGCTTATAATGCAAGATTTTCATTTGAACAGGCATTA-TCT-----TTATTGTAATTTCAGAAA-GCCGTCTTCTTTGAGCTCCGCTCGT-------GATGACGTAGTAA---CTATTGGGT
droPer2	scaffold_2:8077498-8077663 -		CCTGAACCATCCGAAGAGCAGTCTGAAAACATTCCGCCTAAGAAGACAACGTGGGTATATTTGCTTATAATGCAAGATTTTCATTTTAACAGGCATTA-TCT-----TTATTGTAATTTCAGAAA-GCCGTCTTCTTTGAGCTCCGCTCGT-------GATGACGTAGTAA---CTATTGGGT
droWil2	scf2_1100000004585:351397-351539 -		TCTTTCCGTCG--------------------TGCCCCCAATATATAAAAAGTAAGTT-ATTGGATTTAAA-------TGTTCGCTTAAATAATAATTTGTCATAATT-------AATTACAGCAAGAACATGATATTAGATAG--TTGCACGACGGATTATAAAAGGAAAA---CCCCCGAGT
droMoj3	scaffold_6496:20560094-20560096 +		GGT------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droAna3	scaffold_13266:150199-150322 +		GGAAAAGAG----------------------AACCGCCCA--GAAACAAAGTGAGTG-GCTAGCTTTTGATAGAAAGTTC-CTTATGAACAATTT------CTACTT-------CCTTTCAGTAA---TAATA-ATTTGGACT------AT-------GATGTCGTGGAGG---ACTTAGGGT
droFic1	scf7180000453201:4495-4518 -		CCAA--CGAACTGAGGAAAAGCCTGA-------------------------------------------------------------------------------------------------------------------------------------------------------------
droEle1	scf7180000491201:1550832-1550857 -		CAAGAGGGACATGAAAAACAACCAG-------------------------------------------------------------------------------------------------------------------------------------------------------------A
droTak1	scf7180000415763:178650-178675 +		TGAT-------------------------------------------------------------------------------------------------------------------------------------------------------------GTTGAAGTAATCGTTGGAAGGT
droSim2	2r:6558706-6558779 +		ATTT-------------------------------------------------------------------------------------------------------TTTCCATAGTTTTAA--A-ACCATTATTTTAGGACTTCCTTTGTGACAGAT---GTTGAAGTGGACCTTAAAAAGT
droSec2	scaffold_1:3392157-3392230 +		ATTG-------------------------------------------------------------------------------------------------------TTTCCATAATTTTAA--A-ACCACTTTTTTAGGACTTCCTTTGTGACAGAT---GTTGAAGTGGACCTTAAAAAGT
droYak3	2L:18408076-18408082 +		TGAGAAC--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droEre2	scaffold_4929:8586335-8586349 -		CCCAAACGATCCAAA------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

dp5

CCTGAACCATCCGAAGAGCAGTCTGAAAACATTCCGCCTAAGAAGACAACGTGGGTATATTTGCTTATAATGCAAGATTTTCATTTGAACAGGCATTA-TCT-----TTATTGTAATTTCAGAAA-GCCGTCTTCTTTGAGCTCCGCTCGT-------GATGACGTAGTAA---CTATTGGGT	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
...........................................................................................................................................AGCTCCGCTCGT-------GATGACGTAG...............	22	1	1.00	1	0	0	0	0	1	0	0	0	0	0

droPer2

CCTGAACCATCCGAAGAGCAGTCTGAAAACATTCCGCCTAAGAAGACAACGTGGGTATATTTGCTTATAATGCAAGATTTTCATTTTAACAGGCATTA-TCT-----TTATTGTAATTTCAGAAA-GCCGTCTTCTTTGAGCTCCGCTCGT-------GATGACGTAGTAA---CTATTGGGT	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
No Reads

droWil2

TCTTTCCGTCG--------------------TGCCCCCAATATATAAAAAGTAAGTT-ATTGGATTTAAA-------TGTTCGCTTAAATAATAATTTGTCATAATT-------AATTACAGCAAGAACATGATATTAGATAG--TTGCACGACGGATTATAAAAGGAAAA---CCCCCGAGT	Size	Hit Count	Total Norm	Total	M020 Head	M045 Female-body	V117 Male-body	V118 Embryo	V119 Head
......................................................................................................................................ATTAGATAG--TTGCACGACGGAT.........................	22	1	1.00	1	0	0	1	0	0

droMoj3

GGT------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
No Reads

droAna3

GGAAAAGAG----------------------AACCGCCCA--GAAACAAAGTGAGTG-GCTAGCTTTTGATAGAAAGTTC-CTTATGAACAATTT------CTACTT-------CCTTTCAGTAA---TAATA-ATTTGGACT------AT-------GATGTCGTGGAGG---ACTTAGGGT	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
......................................CA--GAAACAAAGTGAGTG-GCTAGCTTTT...................................................................................................................	27	1	1.00	1	0	1	0	0	0	0

droFic1

CCAA--CGAACTGAGGAAAAGCCTGA-------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total
No Reads

droEle1

CAAGAGGGACATGAAAAACAACCAG-------------------------------------------------------------------------------------------------------------------------------------------------------------A	Size	Hit Count	Total Norm	Total
No Reads

droTak1

TGAT-------------------------------------------------------------------------------------------------------------------------------------------------------------GTTGAAGTAATCGTTGGAAGGT	Size	Hit Count	Total Norm	Total
No Reads

droSim2

ATTT-------------------------------------------------------------------------------------------------------TTTCCATAGTTTTAA--A-ACCATTATTTTAGGACTTCCTTTGTGACAGAT---GTTGAAGTGGACCTTAAAAAGT	Size	Hit Count	Total Norm	Total	M023 Head	M024 Male-body	M025 Embryo	M053 Female-body	O001 Testis	O002 Head	SRR553485 Ovary	SRR553486 Ovary	SRR553487 Ovary	SRR553488 Ovary	SRR618934 Ovary	V044 Embryo	SRR902008 Ovary	SRR902009 Testis	SRR1275487 Male larvae	SRR1275485 Male prepupae	SRR1275483 Male prepupae
......................................................................................................................................................TGACAGAT---GTTGAAGTGGACCTTAAAA...	27	1	2.00	2	0	0	0	0	0	0	0	2	0	0	0	0	0	0	0	0	0
..................................................................................................................................................TTTGTGACAGAT---GTTGAAGTGA............	22	1	1.00	1	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	0
...............................................................................................................................................TCCTTTGTGACAGAT---GTTGAAGTGGACCT........	29	1	1.00	1	0	0	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0

droSec2

ATTG-------------------------------------------------------------------------------------------------------TTTCCATAATTTTAA--A-ACCACTTTTTTAGGACTTCCTTTGTGACAGAT---GTTGAAGTGGACCTTAAAAAGT	Size	Hit Count	Total Norm	Total	M054 Female-body	V113 Male-body	V114 Embryo	V115 Head
No Reads

droYak3

TGAGAAC--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Size

Hit Count

Total Norm

Total

M026
Head

M043
Female-body

M056
Embryo

V046
Embryo

V052
Head

V058
Head

V120
Male-body

SRR1275488
Male larvae

SRR1275486
Male prepupae

SRR1275484
Male prepupae

GSM1528802
follicle cells

No Reads

droEre2

CCCAAACGATCCAAA------------------------------------------------------------------------------------------------------------------------------------------------------------------------	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
No Reads

dps_1204

3:13541974-13542039 +

Candidate

Unknown

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads