dps-mir-2529

TGGATAATTACGTGTGTGTCAGTAGGCTGCCTCCTTGTCTGATAGGTACTGCCGGTTTTGGGCAAAATTATCGTGTCACTCATTATTCAACGCATTTTTACTTCGGGGAAGGCAAAATGCGTAGAAAAGTGGGTGACAGGATAATGTTGGATCGAAGCGGGCAAGACATACATAGACGAGCGGACAGCTTACTGAAACTTGCGTATTTCTCCA ************************************************(((.(((((((.(((.((((((.(((((((((((.(((.(((((((((..((((...))))..))))))))).))).))))))))))).)))))).)))..))))))).))).************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M040 female body	GSM444067 head	M062 head	V112 male body	GSM343916 embryo	M059 embryo	SRR902012 CNS imaginal disc
TGGATAATTACGTGAGTGTCA................................................................................................................................................................................................	21	1	1	36.00	36	13	16	1	5	1	0	0
..............................................................................................................................AAGTGGGTGACAGGATAATGTT.................................................................	22	0	1	33.00	33	24	4	4	0	1	0	0
..............................................................................................................................AAGTGGGTGACAGGATAATGT..................................................................	21	0	1	24.00	24	6	12	1	5	0	0	0
.................................................................AATTATCGTGTCACTCATTATT..............................................................................................................................	22	0	1	23.00	23	11	7	5	0	0	0	0
..............................................................................................................................AAGTGGGTGACAGGATAATG...................................................................	20	0	1	6.00	6	2	3	0	1	0	0	0
.................................................................AATTATCGTGTCACTCATTAT...............................................................................................................................	21	0	1	5.00	5	0	2	0	3	0	0	0
TGGATAATTACGTGAGTGTC.................................................................................................................................................................................................	20	1	1	5.00	5	1	2	2	0	0	0	0
.................................................................AGTTATCGTGTCACTCATTATT..............................................................................................................................	22	1	1	2.00	2	0	1	1	0	0	0	0
..............................................................................................................................AAGTGGGTGACAGGATAATGTTT................................................................	23	1	1	2.00	2	1	0	1	0	0	0	0
..............................................................................................................................AAGTGGGTGACAGGATAAT....................................................................	19	0	1	2.00	2	2	0	0	0	0	0	0
.................................................................AATTATCGTGTCACTCATTA................................................................................................................................	20	0	1	2.00	2	1	1	0	0	0	0	0
.......................................TGATAGGTACTGCCGGTTTTGGGC......................................................................................................................................................	24	0	1	1.00	1	0	0	0	0	0	1	0
..............................................................................................................................AAGTGGGTGACAGGATAATGTTG................................................................	23	0	1	1.00	1	0	1	0	0	0	0	0
..................................................................ATTATCGTGTCACTCATTATT..............................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0	0
........................................................................................................................................CAGGATAATGTTGGATCGAAGCGGGC...................................................	26	0	1	1.00	1	0	0	0	0	0	0	1
.................................................................AAGTATCATGTCACTCATTATT..............................................................................................................................	22	2	1	1.00	1	0	0	1	0	0	0	0
..............................................................................................................................AAGTGGGTGACAGGATAATGTTTTT..............................................................	25	3	1	1.00	1	1	0	0	0	0	0	0
..........................................TAGGTACTGCCGGTTTTGGGCA.....................................................................................................................................................	22	0	1	1.00	1	0	1	0	0	0	0	0
..............................................................................................................................AAGTGGGGGACAGGATAATG...................................................................	20	1	1	1.00	1	0	1	0	0	0	0	0
........................................................................................................................................................................TACATAGACGAGCGGACAGCTTACT....................	25	0	1	1.00	1	0	0	0	0	1	0	0
..............................................................CAAAATTATCGTGTCACTCA...................................................................................................................................	20	0	1	1.00	1	1	0	0	0	0	0	0
..............................................................................................................................AAGCGGGTGACAGGATAATG...................................................................	20	1	1	1.00	1	0	0	1	0	0	0	0
TGGATAATTACGTGAGTGT..................................................................................................................................................................................................	19	1	1	1.00	1	0	1	0	0	0	0	0
.......................................................................................................................CGTAGAAAAGTGGGTGACAGG.........................................................................	21	0	1	1.00	1	1	0	0	0	0	0	0
.........................................................................................................................TAGAAAAGTGGGTGACAGGAT.......................................................................	21	0	1	1.00	1	0	0	1	0	0	0	0
..............................................................................................................................AACTGGGTGACAGGATAATGTC.................................................................	22	2	1	1.00	1	0	0	1	0	0	0	0
TGGATAATTACGTGGGTGTCA................................................................................................................................................................................................	21	1	1	1.00	1	1	0	0	0	0	0	0
................................................................................................................................GTGGGTGACAGGATAATGT..................................................................	19	0	1	1.00	1	1	0	0	0	0	0	0
.......................................................................................................................................................................ATACATAGACGAGCGGTGAGCTTATT....................	26	3	1	1.00	1	0	0	0	0	1	0	0
.................................................................AACTATCGTGTCACTCATTATT..............................................................................................................................	22	1	1	1.00	1	0	0	1	0	0	0	0
..............................................................................................................................AAGTGGGTGACAGGATAATGTTTT...............................................................	24	2	1	1.00	1	1	0	0	0	0	0	0
TGCATAATTACGTGAGTGTCA................................................................................................................................................................................................	21	2	1	1.00	1	0	0	1	0	0	0	0
TGAATAATTACGTGAGTGTCA................................................................................................................................................................................................	21	2	1	1.00	1	0	1	0	0	0	0	0
.................................................................AATTATCGTGTCACTCATT.................................................................................................................................	19	0	1	1.00	1	0	0	1	0	0	0	0
.................................................................AATAATCGTGTCACTCATTATT..............................................................................................................................	22	1	1	1.00	1	0	0	1	0	0	0	0
TGGATAATTACGTGAGTGGCA................................................................................................................................................................................................	21	2	1	1.00	1	0	1	0	0	0	0	0
.GAATAATTACGTGAGTGTCAG...............................................................................................................................................................................................	21	2	2	0.50	1	1	0	0	0	0	0	0
TGCATAATTACGTGAGTGTAA................................................................................................................................................................................................	21	3	2	0.50	1	0	1	0	0	0	0	0
TGGATAATTACGTGAGCGGCA................................................................................................................................................................................................	21	3	5	0.20	1	0	1	0	0	0	0	0
..............TGAGGCAGTAGGCTGCATCC...................................................................................................................................................................................	20	3	8	0.13	1	0	1	0	0	0	0	0
.............................................................................................ATCGTGACTTCGGGGAAGG.....................................................................................................	19	3	9	0.11	1	0	0	0	1	0	0	0
..............................................................................................................................AAGTTGGTGGCAGGATAA.....................................................................	18	2	11	0.09	1	1	0	0	0	0	0	0
..............................................................................................................................AAGTAGTGGACAGGATAATG...................................................................	20	3	11	0.09	1	0	1	0	0	0	0	0

ACCTATTAATGCACACACAGTCATCCGACGGAGGAACAGACTATCCATGACGGCCAAAACCCGTTTTAATAGCACAGTGAGTAATAAGTTGCGTAAAAATGAAGCCCCTTCCGTTTTACGCATCTTTTCACCCACTGTCCTATTACAACCTAGCTTCGCCCGTTCTGTATGTATCTGCTCGCCTGTCGAATGACTTTGAACGCATAAAGAGGT ************************************************(((.(((((((.(((.((((((.(((((((((((.(((.(((((((((..((((...))))..))))))))).))).))))))))))).)))))).)))..))))))).))).************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M040 female body
..............................GAGGGTCAGAATATCCATG....................................................................................................................................................................	19	3	10	0.10	1	1

Species	Coordinate	ID	Type	Alignment
dp5	3:10934540-10934752 +	dps-mir-2529	Known_mirbase	TGGA-TAATTACGTGTGTGTCAGTAGGCTGCCTCCTTGTCT-----GATAGGTACTGCCGGTTTTGGGCAAAATTATCGTGTCACTCATTATTCAACGCATTTTTACTTCGGGGAAGGCA-AAATGCGTAGAAAAGTGGGTGACAGGATAATGTTGGATCGAAGCGGGCAAGACATACATAGACGAGCGGACAGCTTACTGAAACTTGCGTATTTCTCCA
droPer2	scaffold_4:6258241-6258453 +			TGGA-AAACTACGTGAGTGTCAGTAGGCTGCCTCCTTGTCT-----GATAGGTACTGCCGGTTTTGGGCAAAAGTATCATATCACTCATTATTCAACGCATTTTTACTTCGGGGAAGGCA-AAATGCGTAGAAAAGTGGGTGACAGGATAATGTTGGATCGAACCGGGCAAGACATACATAGACGAGCGGACAGCTTACTGAAACTTGCGTATTTCTCCA
droVir3	scaffold_12928:7518740-7518768 +			CAAT-T----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGCTAGACTTTGCATATTGTCTCA
droMoj3	scaffold_6680:5819878-5819895 +			AACA-TAC---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GTATGTATACA
droGri2	scaffold_15110:22896292-22896358 +			CGGG-GG------------------------------------------------------------------------------------------------------------GGGGA-AAGTGTATGGAAAAGTGGGTCACATAGCGATTTG---------------------------------------AGCTTAAGGATCGA----TGGGGACA
droAna3	scaffold_12947:1562760-1562794 -			AGTG-TA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAACGCAGTGAAGGAGACATATTGCTTGG
droBip1	scf7180000396769:996421-996508 -			GTCAACAATCATCCAACACAAT-----------------------------------------------------------------------------------------GCAAATGAACAAAT-GAGGGAAAAGTGGGTGGCAGGGCCATGGCTGAGCCAAGAGTCCGAGGCACA------------------------------------------C
droKik1	scf7180000302395:444176-444216 +			CTTG-TTATTA-----------------------------------------------------------------------------------TAGGCTTCCTTACTTCAGAGATGGCA-AAAT----------------------------------------------------------------------------------------------G
droFic1	scf7180000453948:1625524-1625549 -			TGTG-TATGTGTGTGTGTGTGGGT-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GGG
droEle1	scf7180000491028:1300547-1300613 -			TGTA-CAATCGTGCGCGAGTGTGTAGTTTTTTTTCTGGTGTAAGTGTGTAGGTGTGACCGCTTAT--------------------------------------------------------------------------------------------------------------------------------------------------------GGG
droRho1	scf7180000777227:411697-411759 +			TTAG-AA--------------------------------------------------------------------------------------------------------------------------------------------------------CAAAGCGAAGACAACGCACCAAACAAAGCGAACTACTCACTCAAATCTGCGTAT----TTC
droBia1	scf7180000301495:282092-282102 -			TGGA-GGATTA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------C
dm3	chr2R:801516-801525 -			TGGA-TAACTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
droSim2	2l:18941654-18941705 +			AAAA-CG-----------------------------------------------------------------------------------------------------------------------CGTTGACAAGTGGGTGAGGGGGGCGGTTTGGATGGGGGTGGGT------------------------------------------------GGG
droEre2	scaffold_4770:12850310-12850357 -			AAAT-G------------------------------------------------CTGCCTGTTTTGGGCTAACATCTCGAGTCCATCTTTAATC---------------------------------------------------------------------------------------------------------------------------AAC

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

dp5

TGGA-TAATTACGTGTGTGTCAGTAGGCTGCCTCCTTGTCT-----GATAGGTACTGCCGGTTTTGGGCAAAATTATCGTGTCACTCATTATTCAACGCATTTTTACTTCGGGGAAGGCA-AAATGCGTAGAAAAGTGGGTGACAGGATAATGTTGGATCGAAGCGGGCAAGACATACATAGACGAGCGGACAGCTTACTGAAACTTGCGTATTTCTCCA	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
.....................................................................................................................................AAGTGGGTGACAGGATAATGTT.................................................................	22	1	33.00	33	0	24	0	4	1	4	0	0	0	0
.....................................................................................................................................AAGTGGGTGACAGGATAATGT..................................................................	21	1	24.00	24	0	6	0	1	0	12	5	0	0	0
.......................................................................AATTATCGTGTCACTCATTATT...............................................................................................................................	22	1	23.00	23	0	11	0	5	0	7	0	0	0	0
.....................................................................................................................................AAGTGGGTGACAGGATAATG...................................................................	20	1	6.00	6	0	2	0	0	0	3	1	0	0	0
.......................................................................AATTATCGTGTCACTCATTAT................................................................................................................................	21	1	5.00	5	0	0	0	0	0	2	3	0	0	0
.....................................................................................................................................AAGTGGGTGACAGGATAATGTTT................................................................	23	1	2.00	2	0	1	0	1	0	0	0	0	0	0
.....................................................................................................................................AAGTGGGTGACAGGATAAT....................................................................	19	1	2.00	2	0	2	0	0	0	0	0	0	0	0
.......................................................................AATTATCGTGTCACTCATTA.................................................................................................................................	20	1	2.00	2	0	1	0	0	0	1	0	0	0	0
........................................T-----GATAGGTACTGCCGGTTTTGGGC.......................................................................................................................................................	24	1	1.00	1	0	0	1	0	0	0	0	0	0	0
.....................................................................................................................................AAGTGGGTGACAGGATAATGTTG................................................................	23	1	1.00	1	0	0	0	0	0	1	0	0	0	0
........................................................................ATTATCGTGTCACTCATTATT...............................................................................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................................................................CAGGATAATGTTGGATCGAAGCGGGC...................................................	26	1	1.00	1	0	0	0	0	0	0	0	0	0	1
.....................................................................................................................................AAGTGGGTGACAGGATAATGTTTTT..............................................................	25	1	1.00	1	0	1	0	0	0	0	0	0	0	0
................................................TAGGTACTGCCGGTTTTGGGCA......................................................................................................................................................	22	1	1.00	1	0	0	0	0	0	1	0	0	0	0
...............................................................................................................................................................................TACATAGACGAGCGGACAGCTTACT....................	25	1	1.00	1	0	0	0	0	1	0	0	0	0	0
....................................................................CAAAATTATCGTGTCACTCA....................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..............................................................................................................................CGTAGAAAAGTGGGTGACAGG.........................................................................	21	1	1.00	1	0	1	0	0	0	0	0	0	0	0
................................................................................................................................TAGAAAAGTGGGTGACAGGAT.......................................................................	21	1	1.00	1	0	0	0	1	0	0	0	0	0	0
.......................................................................................................................................GTGGGTGACAGGATAATGT..................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.....................................................................................................................................AAGTGGGTGACAGGATAATGTTTT...............................................................	24	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.......................................................................AATTATCGTGTCACTCATT..................................................................................................................................	19	1	1.00	1	0	0	0	1	0	0	0	0	0	0

droPer2

TGGA-AAACTACGTGAGTGTCAGTAGGCTGCCTCCTTGTCT-----GATAGGTACTGCCGGTTTTGGGCAAAAGTATCATATCACTCATTATTCAACGCATTTTTACTTCGGGGAAGGCA-AAATGCGTAGAAAAGTGGGTGACAGGATAATGTTGGATCGAACCGGGCAAGACATACATAGACGAGCGGACAGCTTACTGAAACTTGCGTATTTCTCCA	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
TGGA-AAACTACGTGAGTGTCA......................................................................................................................................................................................................	21	1	204.00	204	0	6	0	48	94	56
.....................................................................................................................................AAGTGGGTGACAGGATAATGTT.................................................................	22	1	10.00	10	0	1	1	2	4	2
....................................................................................................................................................................................AGACGAGCGGACAGCTTACT....................	20	1	9.00	9	0	0	0	3	6	0
TGGA-AAACTACGTGAGTGTC.......................................................................................................................................................................................................	20	1	8.00	8	0	0	0	2	6	0
.....................................................................................................................................AAGTGGGTGACAGGATAATGT..................................................................	21	1	8.00	8	0	0	0	0	5	3
.........................................................................................................................................................................................................AAACTTGCGTATTTCTCCA	19	1	4.00	4	0	0	0	0	4	0
....................................................................................................................................................................................AGACGAGCGGACAGCTTACTGT..................	22	1	3.00	3	0	0	0	1	2	0
.....................................................................................................................................AAGTGGGTGACAGGATAATG...................................................................	20	1	3.00	3	0	0	0	0	3	0
.....................................................................................................................................................................................GACGAGCGGACAGCTTACTGTT.................	22	1	2.00	2	0	0	0	1	1	0
TGGA-AAACTACGTGAGTGT........................................................................................................................................................................................................	19	1	2.00	2	0	0	0	0	0	2
.....................................................................................................................................................AATGTTGGATCGAACCGGGC...................................................	20	1	2.00	2	2	0	0	0	0	0
....................................................................................................................................................TAATGTTGGATCGAACCGGGCAAGAC..............................................	26	1	2.00	2	2	0	0	0	0	0
TGGA-AAACTACGTGAGTGTCT......................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0
.....................................................................................................................................AAGTGGGTGACAGGATAATT...................................................................	20	1	1.00	1	0	0	0	0	1	0
.........................................................................................................................................................................AAGACATACATAGACGAGCGGACA...........................	24	1	1.00	1	1	0	0	0	0	0
.....................................................................................................................................................................................GACGAGCGGACAGCTTACTGT..................	21	1	1.00	1	0	0	0	0	1	0
TGGA-AAACTACGTGAGTGTCC......................................................................................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0
.....................................................................................................................................AAGTGGGTGACAGGATAATGTTG................................................................	23	1	1.00	1	0	0	0	0	1	0
....................................................................................................................................................................................AGACGAGCGGACAGCTTACTG...................	21	1	1.00	1	0	0	0	0	1	0
.GGA-AAACTACGTGAGTGTCA......................................................................................................................................................................................................	20	1	1.00	1	0	1	0	0	0	0
..............................................................TTTGGGCAAAAGTATCATATC.........................................................................................................................................	21	1	1.00	1	1	0	0	0	0	0
.....................................................................................................................................................................................GACGAGCGGACAGCTTACTG...................	20	1	1.00	1	0	0	0	1	0	0
TGGA-AAACTACGTGAGTGTCAA.....................................................................................................................................................................................................	22	1	1.00	1	0	0	0	1	0	0
......................GTAGGCTGCCTCCTTGTCT-----GA............................................................................................................................................................................	21	1	1.00	1	0	0	0	0	1	0

droVir3

CAAT-T----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGCTAGACTTTGCATATTGTCTCA

Size

Hit Count

Total Norm

Total

M027

Male-body

M028

Head

M047

Female-body

M061

Embryo

SRR060654

Ovary

SRR060655

Testis

SRR060656

Ovary

SRR060657

Testis

SRR060658

Ovary

SRR060659

Testis

SRR060660

Ovary

SRR060661

Embryo

SRR060662

Embryo

SRR060663

Embryo

SRR060664

Male-body

SRR060665

Female-body

SRR060666

Male-body

SRR060667

Female-body

SRR060668

Male-body

SRR060669

Female-body

SRR060670

Testis

SRR060671

Male-body

SRR060672

Female-body

SRR060673

Ovary

SRR060674

Ovary

SRR060675

Ovary

SRR060676

Ovary

SRR060677

Ovary

SRR060678

Testis

SRR060679

Testis

SRR060680

Testis

SRR060681

Testis

SRR060682

Embryo

SRR060683

Testis

SRR060684

Embryo

SRR060685

Embryo

SRR060686

Embryo

SRR060687

Embryo

SRR060688

Ovary

SRR060689

Testis

SRR1106712

embryo_0-2h

SRR1106713

embryo_0-2h

SRR1106714

embryo_2-4h

SRR1106715

embryo_4-6h

SRR1106716

embryo_4-6h

SRR1106717

embryo_6-8h

SRR1106718

embryo_6-8h

SRR1106719

embryo_8-10h

SRR1106720

embryo_8-10h

SRR1106721

embryo_10-12h

SRR1106722

embryo_10-12h

SRR1106723

embryo_12-14h

SRR1106724

embryo_12-14h

SRR1106725

embryo_14-16h

SRR1106726

embryo_14-16h

SRR1106727

larvae

SRR1106728

larvae

SRR1106729

mixed whole adult body

SRR1106730

embryo_16-30h

V047

Embryo

V053

Head

V116

Male-body

GSM1528803

follicle cells

SRR2096013

Ovary_Strain_9_oxidized

SRR2096014

Ovary_Strain_160_oxidized

SRR2096026

embryo_0-2h from 14 day old Strain 9 mothers

SRR2096038

embryo_0-2h from 14 day old Strain 9 mothers

SRR2096050

embryo_0-2h from 14 day old Strain 160 mothers

SRR2096055

Ovary_Min_43

SRR2096056

Ovary_Min_46

SRR2096057

Ovary_Min_50

SRR2096058

Ovary_Min_98

SRR2096059

Ovary_Min_164

SRR2096060

Ovary_Min_193

SRR2096061

embryo_0-2h from 14 day old Strain 160 mothers

SRR2096072

embryo_0-2h from 15to16 day old dysgenic mothers

SRR2096079

embryo_0-2h from 15to16 day old dysgenic mothers

SRR2096080

embryo_0-2h from 15to16 day old nondysgenic mothers

SRR2096081

embryo_0-2h from 15to16 day old nondysgenic mothers

No Reads

droMoj3

AACA-TAC---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GTATGTATACA	Size	Hit Count	Total Norm	Total	M046 Female-body	V041 Embryo	V049 Head	V056 Head	M060 Embryo	V110 Male-body
No Reads

droGri2

CGGG-GG------------------------------------------------------------------------------------------------------------GGGGA-AAGTGTATGGAAAAGTGGGTCACATAGCGATTTG---------------------------------------AGCTTAAGGATCGA----TGGGGACA	Size	Hit Count	Total Norm	Total	M041 Female-body	V109 Male-body
No Reads

droAna3

AGTG-TA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAACGCAGTGAAGGAGACATATTGCTTGG	Size	Hit Count	Total Norm	Total	M044 Female-body	M058 Embryo	V039 Embryo	V055 Head	V105 Male-body	V106 Head
AGTG-TA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAACGCAGTGAAGG...............	20	20	0.05	1	0	0	1	0	0	0

droBip1

GTCAACAATCATCCAACACAAT-----------------------------------------------------------------------------------------GCAAATGAACAAAT-GAGGGAAAAGTGGGTGGCAGGGCCATGGCTGAGCCAAGAGTCCGAGGCACA------------------------------------------C	Size	Hit Count	Total Norm	Total
No Reads

droKik1

CTTG-TTATTA-----------------------------------------------------------------------------------TAGGCTTCCTTACTTCAGAGATGGCA-AAAT----------------------------------------------------------------------------------------------G	Size	Hit Count	Total Norm	Total
No Reads

droFic1

TGTG-TATGTGTGTGTGTGTGGGT-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GGG	Size	Hit Count	Total Norm	Total
No Reads

droEle1

TGTA-CAATCGTGCGCGAGTGTGTAGTTTTTTTTCTGGTGTAAGTGTGTAGGTGTGACCGCTTAT--------------------------------------------------------------------------------------------------------------------------------------------------------GGG	Size	Hit Count	Total Norm	Total
No Reads

droRho1

TTAG-AA--------------------------------------------------------------------------------------------------------------------------------------------------------CAAAGCGAAGACAACGCACCAAACAAAGCGAACTACTCACTCAAATCTGCGTAT----TTC	Size	Hit Count	Total Norm	Total
No Reads

droBia1

TGGA-GGATTA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------C	Size	Hit Count	Total Norm	Total
No Reads

dm3

TGGA-TAACTA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Size

Hit Count

Total Norm

Total

GSM154618

Ovary

GSM154620

Ovary

GSM154621

Ovary

GSM154622

Ovary

GSM180328

Head

GSM180329

Mixed-body

GSM180330

Embryo

GSM180331

Embryo

GSM180332

Embryo

GSM180333

Embryo

GSM180334

Larvae

GSM180335

Disc

GSM180336

Pupae

GSM180337

S2

GSM239041

Head

GSM239050

Head

GSM239051

S2

GSM239052

S2

GSM246084

Head

GSM266765

S2

GSM280082

Ovary

GSM280083

Ovary

GSM280084

Ovary

GSM280085

Testis

GSM280086

Ovary

GSM280087

S2-NP

GSM280088

S2-NP

GSM312995

Head

GSM313160

Head

GSM313161

Head

GSM313162

Head

GSM313163

Head

GSM313164

Head

GSM313165

Head

GSM343287

Embryo

GSM379050

Ovary

GSM379051

Ovary

GSM379052

Ovary

GSM379053

Ovary

GSM379054

Ovary

GSM379055

Ovary

GSM379056

Ovary

GSM379057

Ovary

GSM379058

Ovary

GSM379059

Ovary

GSM379060

Ovary

GSM379061

Ovary

GSM379062

Ovary

GSM379063

Ovary

GSM379064

Ovary

GSM379065

Ovary

GSM379066

Ovary

GSM379067

Ovary

GSM424739

S2

GSM424740

S2

GSM424741

S2

GSM467729

Female-body

GSM467730

Female-body

GSM467731

Female-body

M002

CS

M003

Male-body

M004

Male-body

M005

ML-DmD32

M006

ML-DmD32

M029

OSC

M030

OSC

M031

OSC

M032

S2-R+

M033

S2-R+

M034

S2-R+

M035

S2-R+

M036

S2-R+

M037

S2-R+

M038

S2-R+

M039

S2-R+

M048

S2-R+

OSS2

OSS

OSS6

OSS

OSS7

OSS

OSS8

OSS

S1

Head

S10

Embryo

S11

Embryo

S12

Embryo

S13

Embryo

S14

Embryo

S15

S2-GMR

S16

Kc

S17

Larvae

S18

Larvae

S19

Pupae

S2

Head

S20

Pupae

S21

Disc

S22

Head

S23

Female-body

S24

Male-body

S25

Larvae

S26

Larvae

S27

Pupae

S28

Pupae

S29

Head

S3

Male-body

S30

S2-GMR

S31

S2

S32

S2

S33

Kc

S34

Kc

S35

Kc

S4

Female-body

S5

Disc

S6

Embryo

S7

Embryo

S8

S2

S9

Embryo

SRR001337

Head

SRR001338

Head

SRR001339

Head

SRR001340

Head

SRR001341

Head

SRR001342

Head

SRR001343

Head

SRR001344

Head

SRR001345

Head

SRR001346

Head

SRR001347

Head

SRR001348

Head

SRR001349

Head

SRR001664

Head

SRR010951

Ovary

SRR010952

Ovary

SRR010953

Ovary

SRR010954

Ovary

SRR010955

Ovary

SRR010956

Ovary

SRR010957

Ovary

SRR010958

Ovary

SRR010959

Ovary

SRR010960

Ovary

SRR014268

Embryo

SRR014269

Embryo

SRR014270

Embryo

SRR014271

Embryo

SRR014272

Embryo

SRR014273

Ovary

SRR014274

Ovary

SRR014275

Ovary

SRR014276

Ovary

SRR014277

Ovary

SRR014278

Ovary

SRR014279

Ovary

SRR014280

Ovary

SRR014281

Ovary

SRR014282

Ovary

SRR023197

S2

SRR023399

S2

SRR023400

S2

SRR023402

S2

SRR023407

S2

SRR029028

S2

SRR029029

S2

SRR029030

S2

SRR029031

S2

SRR029032

S2

SRR029033

S2

SRR029608

Head

SRR029633

Head

SRR030145

gb3

SRR031692

Head

SRR031693

Head

SRR031694

Head

SRR031696

Head

SRR031697

Head

SRR031698

Head

SRR031699

Head

SRR031700

Head

SRR031701

Head

SRR031702

Head

SRR031703

Head

SRR031704

Head

SRR032092

S2

SRR032093

S2

SRR032094

S2

SRR032095

S2-NP

SRR032096

S2-NP

SRR032152

Head

SRR060643

Testis

SRR060644

Ovary

SRR060645

Testis

SRR060646

Ovary

SRR060647

Ovary

SRR060648

Ovary

SRR060649

Ovary

SRR060650

Testis

SRR060651

Ovary

SRR060652

Testis

SRR060653

Ovary

SRR065151

Ovary

SRR065152

Ovary

SRR065153

Ovary

SRR065154

Ovary

SRR065155

Ovary

SRR065800

Ovary

SRR065801

Ovary

SRR065802

Ovary

SRR065803

Ovary

SRR065804

Ovary

SRR065805

Ovary

SRR065806

Ovary

SRR065807

Ovary

SRR097865

S2-NP

SRR097866

S2-NP

SRR097867

S2-NP

SRR298536

Ovary

SRR298537

Ovary

SRR298711

Ovary

SRR298712

Ovary

SRR317110

Ovary

SRR317111

Ovary

SRR317112

Ovary

SRR317113

Ovary

SRR317114

Ovary

SRR317115

Ovary

SRR317116

Ovary

SRR317117

Ovary

SRR341115

S2

SRR341116

S2

SRR341117

S2

SRR341118

S2

SRR351332

Mixed-body

SRR351333

Mixed-body

T004

OSC

T005

CME-L1

V001

Embryo

V002

Embryo

V003

S2

V006

Female-body

V007

Head

V008

S2

V009

CME-L1

V010

ML-DmD20-C5

V011

Sg4

V012

Male-body

V013

Head

V014

DTT

V015

DreRFHV

V018

Kc167

V019

GM2

V020

S2-R+

V021

ML-DmD21

V022

ML-DmD32

V023

Head

V024

Kc167

V025

S2-R+

V026

1182-2H

V027

ML-DmD21

V028

CME-W1-Cl.8+

V029

ML-DmD9

V030

ML-DmD8

V031

GM2

V032

S1

V033

CME-W1-Cl.8+

V034

ML-DmD16-C3

V035

ML-DmD32

V036

ML-DmD20-C5

V037

S2

V038

S2

V063

Ovary

V0632

Ovary

V064

Ovary

V0642

Ovary

V065

Ovary

V0652

Ovary

V066

Ovary

V0662

Ovary

V067

S2-NP

V073

mbn2

V074

S3

V075

ML-DmBG1-C1

V076

ML-DmBG3-C2

V077

Head

V078

Head

V079

Head

V080

Head

V081

Embryo

V082

Embryo

V083

Male-body

V084

Female-body

V085

CME-W2

V086

Female-body

V088

Ovary

V0882

Ovary

V089

Ovary

V0892

Ovary

V090

Male-body

V091

fGS/OSS

V092

Male-body

V093

Male-body

V094

S2-R+

V095

S2-R+

V096

Ovary

V097

Ovary

V098

Ovary

V125

ML-DmD9

V126

CME-L1

V127

G2

V128

S3

V129

ML-DmBG1-C1

V130

ML-DmBG3-C2

V131

ML-DmD16-C3

V132

ML-DmD32

V133

Sg4

V134

ML-DmD8

V135

CME-W2

V136

Male-body

V137

Head

V138

Male-body

V139

Female-body

V140

Female-body

V141

Female-body

V142

Female-body

V144

OSC

V145

S2

V146

S1

V147

1182-2H

V148

mbn2

GSM1528798

follicle cells

No Reads

droSim2

AAAA-CG-----------------------------------------------------------------------------------------------------------------------CGTTGACAAGTGGGTGAGGGGGGCGGTTTGGATGGGGGTGGGT------------------------------------------------GGG

Size

Hit Count

Total Norm

Total

M023

Head

M024

Male-body

M025

Embryo

M053

Female-body

O001

Testis

O002

Head

SRR553485

Ovary

SRR553486

Ovary

SRR553487

Ovary

SRR553488

Ovary

SRR618934

Ovary

V044

Embryo

SRR902008

Ovary

SRR902009

Testis

SRR1275487

Male larvae

SRR1275485

Male prepupae

SRR1275483

Male prepupae

No Reads

droEre2

AAAT-G------------------------------------------------CTGCCTGTTTTGGGCTAACATCTCGAGTCCATCTTTAATC---------------------------------------------------------------------------------------------------------------------------AAC	Size	Hit Count	Total Norm	Total	M055 Female-body	M057 Embryo	V060 Head	V040 Embryo	V107 Male-body	V108 Head	GSM1528801 follicle cells
No Reads

dps-mir-2529

3:10934590-10934702 +

Known

Unknown

intergenic

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads