dps-mir-2522b

CTCGTCGACATATCACCGCATACCAGTGTCCTGTGAGTTTCGGGACTCCTGTGTTTTCTACGATCGAAGTAATTTCCGGGAATGCGCTGTTGTTGGTGTATTCGAAATTCCAGAGCAGAGCATTCTTCGTCACTACCGCCATCAACGAGCATCTCATATCGGAATGTCTTAAATGTCCAAATACTATCATAGTATCCTGTGA ********************************************************..........(((.....(((((((((.(((((.((((.((.......)).))))))))).)))))).)))....))).....***********************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	SRR902011 testis	V112 male body	M040 female body	GSM444067 head	M062 head	GSM343916 embryo	M059 embryo	SRR902010 ovaries	SRR902012 CNS imaginal disc	M022 male body
.........................................................................TTCCGGGAATGCGCTGTTGTT............................................................................................................	21	0	1	64.00	64	44	14	5	1	0	0	0	0	0	0
.........................................................................TTCCGGGAATGCGCTGTTGTTGG..........................................................................................................	23	0	1	51.00	51	25	16	3	3	1	1	1	0	1	0
.........................................................................TTCCGGGAATGCGCTGTTGTTG...........................................................................................................	22	0	1	41.00	41	6	12	18	3	0	1	0	0	1	0
.........................................................................TTCCGGGAATGCGCTGTTG..............................................................................................................	19	0	1	28.00	28	11	14	3	0	0	0	0	0	0	0
.........................................................................TTCCGGGAATGCGCTGTTGTC............................................................................................................	21	1	1	17.00	17	9	4	2	0	1	0	1	0	0	0
.........................................................................TTCCGGGAATGCGCTGTTGT.............................................................................................................	20	0	1	11.00	11	8	3	0	0	0	0	0	0	0	0
.........................................................................TTCCGGGAATGCGCTGTTGTTC...........................................................................................................	22	1	1	8.00	8	2	5	0	0	0	0	0	0	0	1
.........................................................................TTCCGGGAATGCGCTGTTGTTT...........................................................................................................	22	1	1	6.00	6	0	1	2	1	0	0	0	2	0	0
..........................................................................TCCGGGAATGCGCTGTTGTTG...........................................................................................................	21	0	1	4.00	4	3	0	0	1	0	0	0	0	0	0
.........................................................................TTCCGGGAATGCGCTGTTGTTCC..........................................................................................................	23	2	1	4.00	4	0	4	0	0	0	0	0	0	0	0
.........................................................................TTCCGGGAATGCGCTGTTGTTGGA.........................................................................................................	24	1	1	3.00	3	3	0	0	0	0	0	0	0	0	0
.........................................................................TTCCGGGAATGCGCTGTTGTTCT..........................................................................................................	23	2	1	3.00	3	0	3	0	0	0	0	0	0	0	0
..........................................................................TCCGGGAATGCGCTGTTGTT............................................................................................................	20	0	1	3.00	3	1	2	0	0	0	0	0	0	0	0
.........................................................................TTCCGGGAATGCGCTGTTGTTAAA.........................................................................................................	24	3	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.........................................................................TTCCGGGAATGCGCTGTTGTTTTT.........................................................................................................	24	2	1	1.00	1	1	0	0	0	0	0	0	0	0	0
.....................................................................................................TCGAAATTCCAGAGCAGAGCATTCT............................................................................	25	0	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.........................................................................TTCCGAGAATGCGCTGTTG..............................................................................................................	19	1	1	1.00	1	0	0	0	1	0	0	0	0	0	0
....................................................GTTTTCTACGATCGAAGTAAT.................................................................................................................................	21	0	1	1.00	1	1	0	0	0	0	0	0	0	0	0
.........................................................................TTCCGGGAATGCGCTGTTTTT............................................................................................................	21	1	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.......................................................................................................GAAATTCCAGAGCAGAGCATTCTTCCC........................................................................	27	2	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.........................................................................TTACGGGAATGCGCTGTTGTTC...........................................................................................................	22	2	1	1.00	1	0	0	0	0	1	0	0	0	0	0
.........................................................................TTCCGGGAATGCGCTGTTGTTTT..........................................................................................................	23	2	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..........................................................................TCCGGGAATGAGCTGTTGTTG...........................................................................................................	21	1	1	1.00	1	1	0	0	0	0	0	0	0	0	0
.........................................................................TTCCGGGAATGCGCTGTTC..............................................................................................................	19	1	1	1.00	1	0	0	0	0	1	0	0	0	0	0
.........................................................................TTCCGGGAATGCGCTGTTGTTTAA.........................................................................................................	24	3	1	1.00	1	1	0	0	0	0	0	0	0	0	0
.........................................................................TTCCGGGAATGCGCTGTTGTCA...........................................................................................................	22	2	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................................AGAGCAGAGCATTCTTCGTCA......................................................................	21	0	1	1.00	1	0	0	0	1	0	0	0	0	0	0
..........................................................................TTCCGGAATGCGCTGTTGTT............................................................................................................	20	2	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..........................................................................TCCGGGAATGCGCTGTTGT.............................................................................................................	19	0	1	1.00	1	0	0	1	0	0	0	0	0	0	0
..........................................................................TCCGGGAATGCGCTGTTG..............................................................................................................	18	0	1	1.00	1	0	0	1	0	0	0	0	0	0	0
.........................................................................TTCCGGGAATGCGCTGTTGA.............................................................................................................	20	1	1	1.00	1	1	0	0	0	0	0	0	0	0	0
.........................................................................TTCCGGGAATGCGCTGTTGTTAA..........................................................................................................	23	2	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.........................................................................TTCCGGGAACGCGCTGTTGTT............................................................................................................	21	1	1	1.00	1	1	0	0	0	0	0	0	0	0	0
.........................................................................TTCCGGTAATGCGCTGTTGTTGG..........................................................................................................	23	1	1	1.00	1	0	0	0	0	0	1	0	0	0	0
.........................................................................TTCCGGGAATGCGCTGTTGTTTCC.........................................................................................................	24	3	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.........................................................................TTCCGGGAATGCGCTGTTGTTGGT.........................................................................................................	24	0	1	1.00	1	0	1	0	0	0	0	0	0	0	0
...............................................................................................................AGAGCAGAGCATTCTTCGTC.......................................................................	20	0	1	1.00	1	0	0	0	1	0	0	0	0	0	0
...........................................................................................................TTCCAGAGCAGAGCATTCTTCC.........................................................................	22	1	1	1.00	1	1	0	0	0	0	0	0	0	0	0
.............................................ATCCTGTATTTTCTACGAT..........................................................................................................................................	19	2	3	0.33	1	0	0	1	0	0	0	0	0	0	0
................................................................CGAAGTACTTACGGGGAATG......................................................................................................................	20	3	3	0.33	1	0	0	1	0	0	0	0	0	0	0
................................................................................AATGCGCTGTGGTTGGTAAA......................................................................................................	20	3	5	0.20	1	0	0	1	0	0	0	0	0	0	0

GAGCAGCTGTATAGTGGCGTATGGTCACAGGACACTCAAAGCCCTGAGGACACAAAAGATGCTAGCTTCATTAAAGGCCCTTACGCGACAACAACCACATAAGCTTTAAGGTCTCGTCTCGTAAGAAGCAGTGATGGCGGTAGTTGCTCGTAGAGTATAGCCTTACAGAATTTACAGGTTTATGATAGTATCATAGGACACT ***********************************************************..........(((.....(((((((((.(((((.((((.((.......)).))))))))).)))))).)))....))).....********************************************************	Read size	# Mismatch	Hit Count	Total Norm	Total	M040 female body	V112 male body	GSM343916 embryo
.............................................................................................................................AAGCGGTGATGGCGGTAGTAC........................................................	21	3	1	2.00	2	0	2	0
.............................................................................................................................AAGCGGTGATGGCGGTAG...........................................................	18	1	1	1.00	1	1	0	0
............................................................................................................................GAAGCGGTGATGGCGGTAGT..........................................................	20	1	1	1.00	1	1	0	0
............................................................................................................................GAAGCGGTGATGGCGGTAG...........................................................	19	1	1	1.00	1	1	0	0
............................................................................................................................GAAGCGGTGATGGCGGTAGTGC........................................................	22	3	1	1.00	1	1	0	0
............................................TGAGGACACAATAGATACTAAC........................................................................................................................................	22	3	1	1.00	1	1	0	0
.............GTGGCGTATGGTAACAGG...........................................................................................................................................................................	18	1	1	1.00	1	0	0	1
............................................................................................................................CAAGCGGTGATGGCGGTAGTG.........................................................	21	3	2	0.50	1	0	1	0
.........................................................................................................................GAAGAAGCAGTGATGGAGG..............................................................	19	2	7	0.14	1	1	0	0
..............................................................................CCATCCGCGACAATAACCAC........................................................................................................	20	3	8	0.13	1	1	0	0
.........................................................................................................................TAAAAAGCAGTCATGGCG...............................................................	18	2	9	0.11	1	0	0	1

Species	Coordinate	ID	Type	Alignment
dp5	XL_group1e:8363363-8363564 -	dps-mir-2522b	Known_mirbase	CTCGTCGACATATCACCGCATACC----AGTGTCCTGTGAGTTTCGGGACTCCTGTGTTTTCTACGATCGAAGTAATTTCCGGGAATGCGCTGTTGTTGGTGTATTCGAAATTCCAGAGCAGAGCATTCTTCGTCACTACCGCCATCAACGAGCATCTCATATCGGAATGTCTTAAATGTCCAAATACTATCATAGTATCCTGTGA
droPer2	scaffold_13:200888-201082 +	dpe_73	candidate-rescued	CTCGTCGACATG-----------CGGTGAGTGCCCAGAGAGTTTCGGGACTCTTATGTTTTCTACGATCGAAGTAATTTCCGGGAATGCCCTGTTGTTGGTGTATTCGAAATTCCAGAGCAAGGCATTCTCTGTCACTACCGCCATCAACGAGCATCTCATATCGGAATGTCTTAAATGTCTAAATACTATCATAGTATCCTGTGA

Re-alignment of all predicted orthologs with sRNA reads

Species

Read alignment

dp5

CTCGTCGACATATCACCGCATACC----AGTGTCCTGTGAGTTTCGGGACTCCTGTGTTTTCTACGATCGAAGTAATTTCCGGGAATGCGCTGTTGTTGGTGTATTCGAAATTCCAGAGCAGAGCATTCTTCGTCACTACCGCCATCAACGAGCATCTCATATCGGAATGTCTTAAATGTCCAAATACTATCATAGTATCCTGTGA	Size	Hit Count	Total Norm	Total	M022 Male-body	M040 Female-body	M059 Embryo	M062 Head	V043 Embryo	V051 Head	V112 Male-body	SRR902010 Ovary	SRR902011 Testis	SRR902012 CNS imaginal disc
.............................................................................TTCCGGGAATGCGCTGTTGTT............................................................................................................	21	1	64.00	64	0	5	0	0	0	1	14	0	44	0
.............................................................................TTCCGGGAATGCGCTGTTGTTGG..........................................................................................................	23	1	51.00	51	0	3	1	1	1	3	16	0	25	1
.............................................................................TTCCGGGAATGCGCTGTTGTTG...........................................................................................................	22	1	41.00	41	0	18	0	0	1	3	12	0	6	1
.............................................................................TTCCGGGAATGCGCTGTTG..............................................................................................................	19	1	28.00	28	0	3	0	0	0	0	14	0	11	0
.............................................................................TTCCGGGAATGCGCTGTTGTC............................................................................................................	21	1	17.00	17	0	2	1	1	0	0	4	0	9	0
.............................................................................TTCCGGGAATGCGCTGTTGT.............................................................................................................	20	1	11.00	11	0	0	0	0	0	0	3	0	8	0
.............................................................................TTCCGGGAATGCGCTGTTGTTC...........................................................................................................	22	1	8.00	8	1	0	0	0	0	0	5	0	2	0
.............................................................................TTCCGGGAATGCGCTGTTGTTT...........................................................................................................	22	1	6.00	6	0	2	0	0	0	1	1	2	0	0
..............................................................................TCCGGGAATGCGCTGTTGTTG...........................................................................................................	21	1	4.00	4	0	0	0	0	0	1	0	0	3	0
.............................................................................TTCCGGGAATGCGCTGTTGTTCC..........................................................................................................	23	1	4.00	4	0	0	0	0	0	0	4	0	0	0
.............................................................................TTCCGGGAATGCGCTGTTGTTGGA.........................................................................................................	24	1	3.00	3	0	0	0	0	0	0	0	0	3	0
.............................................................................TTCCGGGAATGCGCTGTTGTTCT..........................................................................................................	23	1	3.00	3	0	0	0	0	0	0	3	0	0	0
..............................................................................TCCGGGAATGCGCTGTTGTT............................................................................................................	20	1	3.00	3	0	0	0	0	0	0	2	0	1	0
.............................................................................TTCCGGGAATGCGCTGTTGTTAAA.........................................................................................................	24	1	1.00	1	0	0	0	0	0	0	1	0	0	0
.........................................................................................................TCGAAATTCCAGAGCAGAGCATTCT............................................................................	25	1	1.00	1	0	0	0	0	0	0	1	0	0	0
........................................................GTTTTCTACGATCGAAGTAAT.................................................................................................................................	21	1	1.00	1	0	0	0	0	0	0	0	0	1	0
...........................................................................................................GAAATTCCAGAGCAGAGCATTCTTCCC........................................................................	27	1	1.00	1	0	0	0	0	0	0	1	0	0	0
.............................................................................TTCCGGGAATGCGCTGTTGTTTT..........................................................................................................	23	1	1.00	1	0	0	0	0	0	0	1	0	0	0
.............................................................................TTCCGGGAATGCGCTGTTC..............................................................................................................	19	1	1.00	1	0	0	0	1	0	0	0	0	0	0
.............................................................................TTCCGGGAATGCGCTGTTGTTTAA.........................................................................................................	24	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.............................................................................TTCCGGGAATGCGCTGTTGTCA...........................................................................................................	22	1	1.00	1	0	0	0	0	0	0	1	0	0	0
...................................................................................................................AGAGCAGAGCATTCTTCGTCA......................................................................	21	1	1.00	1	0	0	0	0	0	1	0	0	0	0
..............................................................................TCCGGGAATGCGCTGTTGT.............................................................................................................	19	1	1.00	1	0	1	0	0	0	0	0	0	0	0
..............................................................................TCCGGGAATGCGCTGTTG..............................................................................................................	18	1	1.00	1	0	1	0	0	0	0	0	0	0	0
.............................................................................TTCCGGGAATGCGCTGTTGA.............................................................................................................	20	1	1.00	1	0	0	0	0	0	0	0	0	1	0
.............................................................................TTCCGGGAATGCGCTGTTGTTAA..........................................................................................................	23	1	1.00	1	0	0	0	0	0	0	1	0	0	0
.............................................................................TTCCGGGAATGCGCTGTTGTTTCC.........................................................................................................	24	1	1.00	1	0	0	0	0	0	0	1	0	0	0
.............................................................................TTCCGGGAATGCGCTGTTGTTGGT.........................................................................................................	24	1	1.00	1	0	0	0	0	0	0	1	0	0	0
...................................................................................................................AGAGCAGAGCATTCTTCGTC.......................................................................	20	1	1.00	1	0	0	0	0	0	1	0	0	0	0
...............................................................................................................TTCCAGAGCAGAGCATTCTTCC.........................................................................	22	1	1.00	1	0	0	0	0	0	0	0	0	1	0

droPer2

CTCGTCGACATG-----------CGGTGAGTGCCCAGAGAGTTTCGGGACTCTTATGTTTTCTACGATCGAAGTAATTTCCGGGAATGCCCTGTTGTTGGTGTATTCGAAATTCCAGAGCAAGGCATTCTCTGTCACTACCGCCATCAACGAGCATCTCATATCGGAATGTCTTAAATGTCTAAATACTATCATAGTATCCTGTGA	Size	Hit Count	Total Norm	Total	M021 Embryo	M042 Female-body	V042 Embryo	V050 Head	V057 Head	V111 Male-body
.............................................................................TTCCGGGAATGCCCTGTTGTTGG..........................................................................................................	23	1	296.00	296	0	2	5	22	40	227
.............................................................................TTCCGGGAATGCCCTGTTGTT............................................................................................................	21	1	110.00	110	0	0	0	9	23	78
.............................................................................TTCCGGGAATGCCCTGTTGTTG...........................................................................................................	22	1	101.00	101	0	2	0	12	31	56
.............................................................................TTCCGGGAATGCCCTGTTGTTGGT.........................................................................................................	24	1	18.00	18	0	0	0	1	4	13
.............................................................................TTCCGGGAATGCCCTGTTGTTC...........................................................................................................	22	1	18.00	18	0	0	0	0	0	18
.............................................................................TTCCGGGAATGCCCTGTTGTC............................................................................................................	21	1	16.00	16	0	0	0	0	2	14
.............................................................................TTCCGGGAATGCCCTGTTGT.............................................................................................................	20	1	13.00	13	0	0	0	2	1	10
..............................................................................TCCGGGAATGCCCTGTTGTTGG..........................................................................................................	22	1	10.00	10	0	0	0	0	0	10
..............................................................................TCCGGGAATGCCCTGTTGTTG...........................................................................................................	21	1	9.00	9	0	0	0	0	0	9
.............................................................................TTCCGGGAATGCCCTGTTGTTT...........................................................................................................	22	1	6.00	6	0	0	0	0	0	6
.............................................................................TTCCGGGAATGCCCTGTTG..............................................................................................................	19	1	5.00	5	0	1	0	0	0	4
.............................................................................TTCCGGGAATGCCCTGTTGTTCC..........................................................................................................	23	1	4.00	4	0	0	0	0	0	4
.............................................................................TTCCGGGAATGCCCTGTTGTA............................................................................................................	21	1	3.00	3	0	0	0	0	0	3
.............................................................................TTCCGGGAATGCCCTGTTCC.............................................................................................................	20	1	3.00	3	0	0	0	0	0	3
.............................................................................TTCCGGGAATGCCCTGTTGTTGGA.........................................................................................................	24	1	3.00	3	0	0	0	0	0	3
.............................................................................TTCCGGGAATGCCCTGTTGTCC...........................................................................................................	22	1	2.00	2	0	0	0	0	0	2
.............................................................................TTCCGGGAATGCCCTGTTGTTGT..........................................................................................................	23	1	2.00	2	0	0	0	1	1	0
.............................................................................TTCCGGGAATGCCCTGTTGTTCT..........................................................................................................	23	1	2.00	2	0	0	0	0	0	2
.............................................................................TTCCGGGAATGCCCTGTT...............................................................................................................	18	1	2.00	2	0	0	0	0	0	2
...............................................................................CCGGGAATGCCCTGTTGTTGG..........................................................................................................	21	1	2.00	2	0	0	0	0	0	2
.............................................................................TTCCGGGAATGCCCTGTTGTTGGTG........................................................................................................	25	1	1.00	1	0	0	0	0	0	1
.............................................................................TTCCGGGAATGCCCTGTTGTCCT..........................................................................................................	23	1	1.00	1	0	0	0	0	0	1
..............................................................................TCCGGGAATGCCCTGTTGTTGGT.........................................................................................................	23	1	1.00	1	0	0	0	0	0	1
.........................................................TTTTCTACGATCGAAGTAAT.................................................................................................................................	20	1	1.00	1	0	0	0	0	1	0
..............................................................................TCCGGGAATGCCCTGTTGTT............................................................................................................	20	1	1.00	1	0	0	0	1	0	0
..............................................................................TCCGGGAATGCCCTGTTGTTC...........................................................................................................	21	1	1.00	1	0	0	0	0	0	1
.............................................................................TTCCGGGAATGCCCTGTTGTTTA..........................................................................................................	23	1	1.00	1	0	0	0	0	0	1
.............................................................................TTCCGGGAATGCCCTGTTGTTGAA.........................................................................................................	24	1	1.00	1	0	0	0	0	0	1
..............................................................................TCCGGGAATGCCCTGTTGTTGGTA........................................................................................................	24	1	1.00	1	0	0	0	0	0	1
.............................................................................TTCCGGGAATGCCCTGTTGTCT...........................................................................................................	22	1	1.00	1	0	0	0	0	0	1
.............................................................................TTCCGGGAATGCCCTGTTGTTTC..........................................................................................................	23	1	1.00	1	0	0	0	0	0	1
.............................................................................TTCCGGGAATGCCCTGTTGTTGA..........................................................................................................	23	1	1.00	1	0	0	0	0	0	1
.............................................................................TTCCGGGAATGCCCTGTTGTTGGTGA.......................................................................................................	26	1	1.00	1	0	0	0	0	0	1
.............................................................................TTCCGGGAATGCCCTGTTGTTA...........................................................................................................	22	1	1.00	1	0	0	0	0	0	1
...................................................................................................................AGAGCAAGGCATTCTCTGTCAC.....................................................................	22	1	1.00	1	0	0	0	0	0	1
.............................................................................TTCCGGGAATGCCCTGTTTA.............................................................................................................	20	1	1.00	1	0	0	0	1	0	0
.............................................................................TTCCGGGAATGCCCTGTTGTTTTG.........................................................................................................	24	1	1.00	1	0	0	0	0	0	1
.............................................................................TTCCGGGAATGCCCTGTTGTTGGTGTA......................................................................................................	27	1	1.00	1	0	0	0	0	0	1
.............................................................................TTCCGGGAATGCCCTGTTGTTCTC.........................................................................................................	24	1	1.00	1	0	0	0	0	0	1
................................................................................CGGGAATGCCCTGTTGTTGG..........................................................................................................	20	1	1.00	1	0	0	0	0	1	0
...................................................................................................................AGAGCAAGGCATTCTCTGTCA......................................................................	21	1	1.00	1	0	0	0	0	0	1
.............................................................................TTCCGGGAATGCCCTGTTGTTGGTT........................................................................................................	25	1	1.00	1	0	0	0	0	1	0
...............................................................................CCGGGAATGCCCTGTTGTTG...........................................................................................................	20	1	1.00	1	0	0	0	0	0	1
...................................................................................................................AGAGCAAGGCATTCTCTGTC.......................................................................	20	2	0.50	1	0	1	0	0	0	0
.............................................................................TTCCGGGAATGCCCTGTTTCC............................................................................................................	21	2	0.50	1	0	0	0	0	0	1

dps-mir-2522b

XL_group1e:8363413-8363514 -

Known

Unknown

intergenic

Predicted structure

Flybase annnotation

Sense Strand Reads

Anti-sense strand reads

Re-alignment of all predicted orthologs

Re-alignment of all predicted orthologs with sRNA reads

-19.5	-19.5	-19.1