ID:

dps-mir-2521

 Coordinate:

XL_group1e:8362756-8362842 -

 Confidence:

Known

 Class:

Unknown

 Genomic Locale:

intergenic

 [View on miRBase]

[View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-34.7 -34.5 -34.4

Flybase annnotation

intergenic

No Repeatable elements found

Sense Strand Reads

GCCGTTTCACCCCAATCTCTCGTGTACGATAGTGAGATTCTTGTTTTATAGGATTGACGGTGGGAGGTGATTAGGCCAAATTTCATTGATAGATTCGAAATCCAATAATTTGGCCTAGCATTCGATACCGCAATCCACATATCATTCCAATATCCCGCGAGTGCCCATACTGTTGGGAGTTGATTTC

***********************************...............((((((.(((((...((((.((((((((((((..((((...((((...))))))))))))))))))))))))...)))))))))))................*********************************** 		Read size # Mismatch Hit Count Total Norm Total SRR902011

testis

 V112

male body		 GSM444067

head

 GSM343916

embryo

 M040

female body

 M062

head

 M059

embryo

 SRR902010

ovaries

 SRR902012

CNS imaginal disc
............................................TTTATAGGATTGACGGTGGG........................................................................................................................... 20 0 1 80.00 80 78 1 0 0 0 0 0 0 1
........................................................................................................ATAATTTGGCCTAGCATTCGA.............................................................. 21 0 1 66.00 66 36 5 5 9 3 1 6 1 0
.......................................................................................................AATAATTTGGCCTAGCATTCG............................................................... 21 0 1 26.00 26 9 3 3 3 5 3 0 0 0
........................................................................................................ATAATTTGGCCTAGCATTCG............................................................... 20 0 1 25.00 25 7 6 4 0 4 4 0 0 0
...........................................TTTTATAGGATTGACGGTGGG........................................................................................................................... 21 0 1 25.00 25 24 1 0 0 0 0 0 0 0
.......................................................................................................AATAATTTGGCCTAGCATTCGA.............................................................. 22 0 1 17.00 17 6 1 2 3 4 1 0 0 0
.............................................TTATAGGATTGACGGTGGG........................................................................................................................... 19 0 1 11.00 11 10 1 0 0 0 0 0 0 0
........................................................................................................ATAATTTGGCCTAGCATTCGAA............................................................. 22 1 1 5.00 5 4 0 0 1 0 0 0 0 0
.........................................TGTTTTATAGGATTGACGGTGGG........................................................................................................................... 23 0 1 3.00 3 3 0 0 0 0 0 0 0 0
..........................................GTTTTATAGGATTGACGGTGGG........................................................................................................................... 22 0 1 3.00 3 3 0 0 0 0 0 0 0 0
.......................................................................................................AATAATTTGGCCTAGCATTC................................................................ 20 0 1 3.00 3 0 3 0 0 0 0 0 0 0
.........................................................................................................TAATTTGGCCTAGCATTCGAAA............................................................ 22 1 1 2.00 2 2 0 0 0 0 0 0 0 0
........................................................................................................ATAATTTGGCCTAGCATTCGAT............................................................. 22 0 1 2.00 2 0 0 1 0 0 1 0 0 0
................CTCTAGTGACCGATAGTGA........................................................................................................................................................ 19 3 13 1.38 18 0 1 0 0 17 0 0 0 0
........................................................................................................ATAATTTGCCCTAGCATTCGA.............................................................. 21 1 1 1.00 1 0 0 1 0 0 0 0 0 0
...........................................TTTTATAGGATTGACGGGGGG........................................................................................................................... 21 1 1 1.00 1 1 0 0 0 0 0 0 0 0
........................................................................................................ATAATTTGGCGTAGCATTCGA.............................................................. 21 1 1 1.00 1 0 0 1 0 0 0 0 0 0
.......................................................................................................AATAATTTAGCCTAGCATTCG............................................................... 21 1 1 1.00 1 0 0 1 0 0 0 0 0 0
.......................................................................................................AATAATTTGGCCTAGCATTCGAT............................................................. 23 0 1 1.00 1 0 0 0 0 0 0 1 0 0
.......................................................................................................ATTAATTTGGCCTAGCATTCGA.............................................................. 22 1 1 1.00 1 0 0 0 0 0 0 1 0 0
........................................................................................................AGATTTTGGCCTAGCATTCGAT............................................................. 22 2 1 1.00 1 0 0 0 0 0 0 1 0 0
........................................................................................................ATATTCTGGCCTAGCATTCGA.............................................................. 21 2 1 1.00 1 0 0 1 0 0 0 0 0 0
..............................................TATAGGATTGACGGTGGG........................................................................................................................... 18 0 1 1.00 1 1 0 0 0 0 0 0 0 0
........................................................................................................ACAATTTGGCCTAGCATTCGA.............................................................. 21 1 1 1.00 1 0 0 0 0 0 1 0 0 0
.......................................................................................................AATAAGTTGGCCTAGCATTCG............................................................... 21 1 1 1.00 1 0 0 0 0 0 1 0 0 0
.......................................................................................................AATCATTTGGAGTAGCATTCGA.............................................................. 22 3 1 1.00 1 0 0 0 1 0 0 0 0 0
........................................................................................................AGAATTTGGCCTAGCATTCGA.............................................................. 21 1 1 1.00 1 0 0 0 0 0 1 0 0 0
..............................................TATAGGATTGACGGGGGG........................................................................................................................... 18 1 1 1.00 1 1 0 0 0 0 0 0 0 0
........................................................................................................ATAATTTGGCCGAGCATTCG............................................................... 20 1 1 1.00 1 0 0 1 0 0 0 0 0 0
........................................................................................................ATAATTTGGCCTAGCAGTTGA.............................................................. 21 2 1 1.00 1 0 0 0 1 0 0 0 0 0
............................................TTTATAGGATTGACGGTGG............................................................................................................................ 19 0 1 1.00 1 1 0 0 0 0 0 0 0 0
........................................................................................................ATAATTTGGCCTAGCATTCGAGA............................................................ 23 1 1 1.00 1 0 1 0 0 0 0 0 0 0
...........................................TTTTATAGGCTTGCCGGTGGG........................................................................................................................... 21 2 2 0.50 1 1 0 0 0 0 0 0 0 0
................................TGAGATTCTTCTTTTATAGTTT..................................................................................................................................... 22 3 5 0.20 1 0 0 0 1 0 0 0 0 0
........................................................................................................AGAATTTGGTCTAGCATTCGT.............................................................. 21 3 6 0.17 1 0 0 0 1 0 0 0 0 0
...............TCTCTAGTGACCGATAGTG......................................................................................................................................................... 19 3 14 0.14 2 0 0 0 0 2 0 0 0 0
.......................................................................................................................ATTCGATACAGCACCCCACA................................................ 20 3 8 0.13 1 0 0 0 1 0 0 0 0 0
...............TCTCTAGTGACCGATAGTGA........................................................................................................................................................ 20 3 9 0.11 1 0 0 0 0 1 0 0 0 0
GCCGTGTCTCCCCTATCTCT....................................................................................................................................................................... 20 3 12 0.08 1 0 0 1 0 0 0 0 0 0
.................TCTAGTGACCGATAGTGA........................................................................................................................................................ 18 3 20 0.05 1 0 0 0 0 1 0 0 0 0

Anti-sense strand reads

CGGCAAAGTGGGGTTAGAGAGCACATGCTATCACTCTAAGAACAAAATATCCTAACTGCCACCCTCCACTAATCCGGTTTAAAGTAACTATCTAAGCTTTAGGTTATTAAACCGGATCGTAAGCTATGGCGTTAGGTGTATAGTAAGGTTATAGGGCGCTCACGGGTATGACAACCCTCAACTAAAG

***********************************...............((((((.(((((...((((.((((((((((((..((((...((((...))))))))))))))))))))))))...)))))))))))................*********************************** 		Read size # Mismatch Hit Count Total Norm Total M040

female body

 V112

male body		 GSM444067

head

 M062

head

 GSM343916

embryo
..............................................................................................................................TGGCGATAGGTGTGGAGTA.......................................... 19 3 20 17.15 343 211 115 14 3 0
..............................................................................................................................TGGCGATAGGTGTAGAGTAGGGT...................................... 23 3 1 1.00 1 1 0 0 0 0
..............................................................................................................................TGGCGATAGGTGTGTAGTAGGG....................................... 22 3 2 0.50 1 1 0 0 0 0
..............................................................................................................................TGGCGTTAGGTGTGGAGT........................................... 18 2 5 0.20 1 0 0 1 0 0
..............................................................................................................................TGGCGATAGGTGTGGAGTAA......................................... 20 3 10 0.20 2 2 0 0 0 0
.................................................................................................................................CGTTAGGTCGATAGTATGGTT..................................... 21 3 5 0.20 1 1 0 0 0 0
..............................................................................................................................TGGCGATAGGTGTGCAGTA.......................................... 19 3 7 0.14 1 0 1 0 0 0
..............................................................................................................................TGGCGATAGGTGTGGAGTAAG........................................ 21 3 8 0.13 1 0 1 0 0 0
..............................................................................................................................TGGCGTTAGGTGTGGAGTAG......................................... 20 3 10 0.10 1 0 0 1 0 0
....................................................................................................................................TAGGTGTACAGTAGGGTTGT................................... 20 3 10 0.10 1 0 0 0 0 1
..............................................................................................................................TGGCGCTAGGTGTGGAGTA.......................................... 19 3 11 0.09 1 0 1 0 0 0
..............................................................................................................................TGGCGATAGGTGTGTAGTAG......................................... 20 3 12 0.08 1 1 0 0 0 0
.................................................................................................TTTAGGTTCTTAAACTGG........................................................................ 18 2 16 0.06 1 1 0 0 0 0
.........................................................................CCGGTTGATGGTAACTATC............................................................................................... 19 3 19 0.05 1 1 0 0 0 0
..............................................................................................................................TGGCGATGGGTGTAGAGTA.......................................... 19 3 20 0.05 1 1 0 0 0 0

Re-alignment of all predicted orthologs

Species Coordinate ID Type Alignment
dp5 XL_group1e:8362706-8362892 - dps-mir-2521 Known_mirbase GCCGTTTCACCCCAATCTCTCGTGTACGATAGTGAGATTCTTGTTTTATAGGATTGACGGTGGGAGGTGATTAGGCCAAATTTCATTGATAGATTCGAAATCCAATAATTTGGCCTAGCATTCGATACCGCAATCCACATATCATTCCAATATCCCGCGAGTGCCCATACTGTTGGGAGTTGATTTC
droPer2 scaffold_13:201553-201728 + dpe_336 confident GCCGTTTCACCCCAATCTATCGTGTACGATCGTGAGGCTCTTTTTTTATAGGATTGACGGTAGGAGGTGATTAGGCCAAATTTCATTGATAGATTCGAAGTCCAATAATTTGGCCTAGCATTCGATACCGCAATCCACATATCATCCCAATATCCCGCGAGTGCCCATACTGTTGG-----------

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
dp5
GCCGTTTCACCCCAATCTCTCGTGTACGATAGTGAGATTCTTGTTTTATAGGATTGACGGTGGGAGGTGATTAGGCCAAATTTCATTGATAGATTCGAAATCCAATAATTTGGCCTAGCATTCGATACCGCAATCCACATATCATTCCAATATCCCGCGAGTGCCCATACTGTTGGGAGTTGATTTCSizeHit CountTotal NormTotalM022

Male-body		M040

Female-body		M059

Embryo		M062

Head		V043

Embryo		V051

Head		V112

Male-body		SRR902010

Ovary		SRR902011

Testis		SRR902012

CNS imaginal disc
............................................TTTATAGGATTGACGGTGGG...........................................................................................................................20180.008000000010781
........................................................................................................ATAATTTGGCCTAGCATTCGA..............................................................21166.006603619551360
.......................................................................................................AATAATTTGGCCTAGCATTCG...............................................................21126.00260503333090
........................................................................................................ATAATTTGGCCTAGCATTCG...............................................................20125.00250404046070
...........................................TTTTATAGGATTGACGGTGGG...........................................................................................................................21125.002500000010240
.......................................................................................................AATAATTTGGCCTAGCATTCGA..............................................................22117.00170401321060
.............................................TTATAGGATTGACGGTGGG...........................................................................................................................19111.001100000010100
........................................................................................................ATAATTTGGCCTAGCATTCGAA.............................................................2215.0050000100040
.........................................TGTTTTATAGGATTGACGGTGGG...........................................................................................................................2313.0030000000030
..........................................GTTTTATAGGATTGACGGTGGG...........................................................................................................................2213.0030000000030
.......................................................................................................AATAATTTGGCCTAGCATTC................................................................2013.0030000003000
........................................................................................................ATAATTTGGCCTAGCATTCGAT.............................................................2212.0020001010000
.......................................................................................................AATAATTTGGCCTAGCATTCGAT.............................................................2311.0010010000000
..............................................TATAGGATTGACGGTGGG...........................................................................................................................1811.0010000000010
............................................TTTATAGGATTGACGGTGG............................................................................................................................1911.0010000000010
droPer2
GCCGTTTCACCCCAATCTATCGTGTACGATCGTGAGGCTCTTTTTTTATAGGATTGACGGTAGGAGGTGATTAGGCCAAATTTCATTGATAGATTCGAAGTCCAATAATTTGGCCTAGCATTCGATACCGCAATCCACATATCATCCCAATATCCCGCGAGTGCCCATACTGTTGG-----------SizeHit CountTotal NormTotalM021

Embryo		M042

Female-body		V042

Embryo		V050

Head		V057

Head		V111

Male-body
........................................................................................................ATAATTTGGCCTAGCATTCGA..............................................................21126.00260107135
........................................................................................................ATAATTTGGCCTAGCATTCG...............................................................20125.002500031012
.......................................................................................................AATAATTTGGCCTAGCATTCG...............................................................21118.0018000288
............................................TTTATAGGATTGACGGTAGG...........................................................................................................................20116.00160000016
.............................................TTATAGGATTGACGGTAGG...........................................................................................................................1918.008000008
.......................................................................................................AATAATTTGGCCTAGCATTCGA..............................................................2214.004001111
...........................................TTTTATAGGATTGACGGTAGG...........................................................................................................................2112.002000002
........................................................................................................ATAATTTGGCCTAGCATTCGAA.............................................................2211.001000001
.......................................................................................................AATAATTTGGCCTAGCATCT................................................................2011.001000001

Generated: 10/20/2015 at 07:04 PM