ID:

sblock180959

 Coordinate:

chrX:9219729-9219977 -

 Confidence:

Known Ortholog

 Type:

three_prime_UTR

 [View on UCSC Genome Browser {Cornell Mirror}]
Legend: mature star mismatch in alignment mismatch in read

Predicted structure

-78.0 -77.8 -77.4 -77.2

Sense Strand Reads

TTTGTAAGAACTACCTCCCCCTCCTCCCCATTCCGCAGTCCAGTGAAAAGCGAAAGGTTATGTCGTCGACCCGGGTACAGTGCGTTTCGAGGCTGTATGTGCTTGTTTTAATCCCCAATCATACGCATTCAGCAACCTAAGGTTTCATTCGAAGTCATTCTGCAGGTTCAGCAATCCACTTAGTTTCGTACTCAGGACCGCGATTAGAACGAACGAAATTTTGCATTTGTCACATTCACATCTCTTCGCCTACAGCACCGAAACGCACTGTCCCCGGGTCGTCAAACACTCCCAGGCGAGATTTAATGTTTTTTTTTTTTTCTTCCTCATTTACTTAGAATCCGTAAGA
***********************************************....((...(((.((....(((((((((.(((((((((((((..((((((((((..(((.........................((((..(((((((((.((((...((....(((.(((((.........................))))))))....)).)))).)))))))))...)))).)))..))))..........))))))..))))))))))))).))))))))).)))))..))********************************************************** 		Read size # Mismatch Hit Count Total Norm Total V144
OSC		 V148
mbn2 		SRR014278
Ovary_rep1_w1118(fem)_x_Har(male)_F1
 V137
Male aged head
 V073
mbn2 		V127
G2		 V142
Oxidation_female_body
 V141
Heat_female_body
 V140
Dessication_female_body
 V136
Male aged body
 SRR298537
strain: vret148-60/vret148-60genotype/variation: Vret Mutant
 V138
Male cold body
 V0642
ago2 ovary, AGO1-IP, reseq
AGO1
 V145
S2-DRSC		 SRX247213
FLAG-IP from S2-R+, FLAG-HA-AGO2
AGO2
 SRR029029
dcr-1 knockdown
 SRR029032
r2d2 knockdown
 SRR060647
A2_ovaries_FLAG-Ago2
AGO2
 GSE24545
CS ovary total RNA
 SRR029033
lacZ knockdown
 SRR032092
mock oxidized
 GSM343832
S2R+ cell		 V077
cold, female head
 GSM628272
ago2[414] ovary total RNA
 V125
ML-DmD9		 V139
Cold_female_body
 SRR032093
ago1 knockdown
 SRR065151
Piwi bound piRNAs from tj GAL4 > RNAi-aub ovaries
 SRR065155
Piwi bound piRNAs from tj GAL4 > RNAi-fs(1)Yb ovaries
 SRR097865
Drosophila S2-NP cells
 SRR060645
yw67c23(2)_testes_total
 SRR029030
dcr-2 knockdown
 SRR298712
strain: bam(delta)86/bam(delta)86genotype/variation: Bam Mutant
 SRR097866
Drosophila S2-NP cells
 GSM609251
aged female head
 V079
Oxidation, female head
 V080
Starvation, female head
 SRR029028
untreated (mock)
 V078
Desiccation, female head
 V015
DreRFHV148h
 SRR097867
Drosophila S2-NP cells
 V091
fGS/OSS total  		 SRR298536
strain: vret148-60/TM6genotype/variation: Vret Heterozygote
 GSM371638
S2-NP		 V126
CME L1 		V132
ML-DmD32 		SRR014281
Ovary_rep1_wK(fem)_x_w1118(male)_F1
 V085
CME W2 wing disc		 V0662
r2d2 ovary, AGO1-IP, reseq
AGO1
 V130
ML-DmBG3-c2 		GSM322543
male head #1
 SRR060652
hs-Penelope_testes_total
 SRR065806
Piwi-IP_squ_mut_ovaries
 GSM609234
CS  male total RNA  
 GSM385821
OSS_s7		 SRR031693
2'-O-methylated small RNAs from Oregon R
 SRR031702
2'-O-methylated small RNAs from r2d2 heterozygous flies
 GSM609231
ago2[414], ovary, AGO1IP
AGO1
 GSM628271
r2d2[1] ovary total RNA  
 V147
1182-4H cell		 SRR014276
Ovary_rep1_NA(fem)_x_Har(male)_F1
 SRR014280
Ovary_rep1_w1118_P
 V086
female body, aged
 V003
dsDcr-1 (katsutomo RNA)
 SRR060643
A2_testes_total
 SRR298711
strain: egg1473/ Df(2R)Dll-Mpgenotype/variation: dSETDB1 Mutant
 V006
r2d2 female: possibly heterozygous
 GSM609219
GM2 cell		 V128
S3 		GSM361908
s2-48 Biological Replicate #2
 SRR032096
AGO2 IP dcr2 knockdown
AGO2
 GSM609247
heat female head
 GSM609237
ago2[414] ovary total RNA
 GSM280087
S2cell (AGO2IP)
AGO2
 GSM379065
Zuc Heterozygote
 SRR014273
Ovary_rep1_Har_P
 SRR060653
hs-Penelope_ ovaries_total
 GSM343833
S2R+ cell		 GSM609248
ML-DmD9 cell		 GSM385744
OSS_s2		 GSM399100
Kc167 cell		 V074
S3		 GSM379056
Krimp Heterozygote
 SRR014275
Ovary_rep1_LK_P
 SRR317115
genotype: fs(1)yb_72/fs(1)yb_4stage: from 5-6 days old flies
 SRR317116
genotype: zuc_hm27/Cyo or zuc_cd761/Cyostage: from 5-6 days old flies
 GSM609232
r2d2[1] ovary total RNA  
 V134
ML-DmD8 		GSM379057
Krimp Mutant
 GSM379058
Piwi Heterozygote
 GSM379064
Vasa Mutant
 GSM272652
S2 -48 Biological Replicate #1
 SRR014274
Ovary_rep1_LK(fem)_x_Har(male)_F1
 SRR014282
Ovary_rep1_wK_P
 SRR031694
2'-O-methylated small RNAs from Oregon R
 SRR341117
transfected with dsRNA targeting nibblercell type: Schneider 2 (S2) cellsoxidation
 V008
S2-DRSC		 GSM379051
Armi Mutant
 GSM379052
Aub Heterozygote
 GSM379066
Zuc Mutant
 GSM385822
OSS_s8		 SRR014277
Ovary_rep1_NA_P
 SRR060646
yw67c23(2)_ovaries_total
 SRR065153
Piwi bound piRNAs from tj GAL4 > RNAi-armi ovaries
 SRR065807
Piwi-IP_squ_het_ovaries
 GSM609220
ML-DmD21 cell		 GSM399101
kc167 cell		 V036
ML-DmD20c5 cell		 GSM609230
CS,ovary,AGO1IP
AGO1
 V0632
wt ovary AGO1-IP, reseq
AGO1
 V135
CME W2 (wing disc line)		 GSM312995
WT, oxidized
 GSM379054
Flam Heterozygote
 GSM385748
OSS_s6		 GSM322533
female head #1
 GSM609242
s2+48 #2
 SRR065803
Piwi-IP_zuc_het(mut)_ovaries
 SRR317110
genotype: Df(3L)armi-delta1/TM3SbStage: from 5-6 days old flies
 SRR317112
genotype: CG4771-delta1/TM3Sbstage: from 5-6 days old flies
 GSM609235
CMEL1		 V133
Sg4 		SRR031698
2'-O-methylated small RNAs from dcr-2 heterozygous flies
 GSM280088
S2cell (AGO1IP)
AGO1
 GSM379053
Aub Mutant
 GSM399107
male body #2
 SRR031701
Total small RNAs from r2d2 heterozygous flies
 SRR031704
2'-O-methylated small RNAs from r2d2 homozygous flies
 SRR060644
A2_ovaries_total
 SRR065152
Piwi bound piRNAs from tj GAL4 > RNAi-piwi ovaries
 SRR065154
Piwi bound piRNAs from tj GAL4 > RNAi-zuc ovaries
 SRR065805
Piwi-IP_zuc_het(H-Y)_ovaries
 GSM609225
ML-DmBG3-C2		 SRX247212
AGO1-IP from S2-R+, FLAG-HA-AGO2
AGO1
 V129
ML-DmBG1-c1		 V131
ML-DmD16-c3 		GSM379050
Armi Heterozygote
 GSM379060
SpnE Heterozygote
 GSM609241
s2+48 #1
 SRR029031
loqs-ORF knockdown
 SRR029608
total small RNAs from hen1 heterozygous flies
 SRR060650
A1_testes_total
 SRR065804
Piwi-IP_zuc_H-Y_ovaries
 GSM609249
ML-DmD21 cell		 GSM609250
ML-DmD32 cell		 GSE24544
dcr-2[L811fsX] ovary total RNA
 V146
S1 cell		 GSM609233
r2d2[1], ovary, AGO1IP
AGO1
 GSM280085
WT testes (18-24nt)
 GSM379055
Flam Mutant
 GSM379059
Piwi Mutant
 GSM379061
Squ Heterozygote
 GSM379062
Squ Mutant
 GSM379063
Vasa Heterozygote
 GSM467731
Dmel_loq_sRNAseq
 SRR010953
Aub heterozygotes, oxidized
 SRR010958
Piwi IP in Ago3 trans-heterozygotes
 SRR029633
total small RNAs from hen1 homozygous flies
 SRR065801
zuc_het(H-Y)_ovaries
 SRR065802
Piwi-IP_zuc_mut_ovaries
 SRR317111
genotype: DF(3L)armi-delta1/DF(3L)armi-delta1Stage: from 5-6 days old flies
 SRR317114
genotype: fs(1)yb_72/Fm6 or fs(1)yb_4/Fm6stage: from 5-6 days old flies
 SRR317117
genotype: zuc_hm27/zuc_cd761stage: from 5-6 days old flies
 SRR341116
transfected with dsRNA targeting luciferasecell type: Schneider 2 (S2) cellsoxidation
 GSM609217
MLDmD20c5		 V0652
dcr2 ovary, AGO1-IP, reseq
AGO1
 SRR031692
Total small RNAs from Oregon R
 GSM280083
dcr-2-/- ovaries (18-29nt)
 GSM343287
Drosophila Toll 10b mutant embryos
 GSM379067
SpnE Mutant
 GSM467729
Dmel_wt_sRNAseq
 GSM467730
Dmel_r2d2_sRNAseq
 GSM399105
disk #2
 GSM399106
female body #2
 GSM272651
S2-48,+48, KC-48, +48 mix
 SRR001339
WT_females_non-beta-eliminated
 SRR001344
dcr-2_beta-eliminated
 SRR010951
Ago3 heterozygotes, oxidized
 SRR010956
Piwi IP in Ago3 heterozygotes
 SRR014279
Ovary_rep1_w1118(fem)_x_wK(male)_F1
 SRR031697
Total small RNAs from dcr-2 heterozygous flies
 SRR031703
Total small RNAs from r2d2 homozygous flies
 SRR317113
genotype: CG47711-delta1/CG4771-delta1stage: from 5-6 days old flies
 SRR341115
transfected with dsRNA targeting nibblercell type: Schneider 2 (S2) cellsoxidation
 GSM609240
IR+ 2-18hr
 V012
Dcr2 male (Katsutomo, whole fly?)
 V022
ML-DmD32 cell		 GSM609221
1182-4H cell		 V031
GM2 cell		 V032
S1 cell		 V038
Felix sample S2 only
 GSM609236
dcr-2[L811fsX], ovary, AGO1IP
AGO1
 GSM609222
ML-DmBG1-C1		 GSM609229
embryo 2-6hr
 GSM609224
female, one day
 V092
dcr-2[G31R] male total RNA  
 V093
loqs-KO, 40A; loqs-PB rescue/TM3  male total RNA
..............................................................TCGTCGACCCGGGTACAGTGC.......................................................................................................................................................................................................................................................................... 21 0 1 2578.00 2578 534 348 94 83 60 58 69 52 54 45 47 43 5 36 25 33 23 45 30 34 39 31 26 11 23 24 23 0 0 24 22 12 19 21 23 21 17 14 16 21 15 3 15 10 15 10 11 12 1 13 0 12 0 6 0 12 5 2 4 7 9 7 7 7 5 9 6 10 10 5 9 9 3 7 6 10 8 8 4 0 9 4 6 6 1 0 1 5 5 4 4 3 5 4 6 5 4 3 5 4 0 3 5 0 0 6 4 5 0 2 4 4 4 0 3 0 1 0 0 2 2 1 1 3 2 0 1 2 0 0 1 2 2 2 1 3 1 2 2 1 2 0 2 1 0 1 2 2 0 2 0 1 1 0 0 0 0 1 0 0 0 0 1 2 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 1 1 0 0 0 1 0 0
..............................................................TCGTCGACCCGGGTACAGTGCG......................................................................................................................................................................................................................................................................... 22 0 1 160.00 160 10 23 5 2 8 9 1 2 0 5 3 1 12 1 2 0 3 0 1 0 0 0 2 1 0 0 0 0 0 3 0 0 1 4 2 1 1 6 0 1 1 1 0 2 2 0 0 0 2 0 6 0 0 1 0 0 0 2 1 1 1 0 0 2 1 2 0 0 1 0 0 0 0 1 0 0 0 0 2 0 0 3 0 0 0 0 0 0 0 0 0 2 0 0 0 2 0 0 0 0 0 1 0 0 0 0 0 1 1 1 0 0 0 0 0 5 2 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..............................................................TCGTCGACCCGGGTACAGTG........................................................................................................................................................................................................................................................................... 20 0 1 128.00 128 42 10 4 2 5 1 1 1 1 1 3 1 1 0 1 0 5 0 0 0 0 0 2 4 0 1 5 0 0 0 4 5 0 0 0 0 1 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 3 0 0 2 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 1 0 0 0 0 2 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
....................................................................ACCCGGGTACAGTGCGTTTCG.................................................................................................................................................................................................................................................................... 21 0 1 109.00 109 48 5 1 1 1 4 0 1 1 0 0 0 10 2 2 0 0 0 4 0 0 1 0 3 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 0 0 3 0 2 0 0 0 0 1 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
....................................................................................................................................................................................................................................................................AAACGCACTGTCCCCGGGTCG.................................................................... 21 0 1 56.00 56 19 16 1 0 0 0 0 1 0 0 0 0 0 0 2 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
......................................TCCAGTGAAAAGCGAAAGGTTATGTCG............................................................................................................................................................................................................................................................................................ 27 0 1 38.00 38 33 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
...............................................................................................................................................................................................................................................................CACCGAAACGCACTGTCCCCG......................................................................... 21 0 1 36.00 36 6 8 0 0 0 0 0 0 0 0 0 0 1 0 0 7 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..........................................................................................................................................................................................................................................................................ACTGTCCCCGGGTCGTCAAACA............................................................. 22 0 1 33.00 33 14 3 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 2 0 0 0 1 0 2 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..............................................................TCGTCGACCCGGGTACAGGGC.......................................................................................................................................................................................................................................................................... 21 1 1 31.00 31 1 15 0 0 2 2 1 1 0 0 1 0 0 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
................................................................GTCGACCCGGGTACAGTGC.......................................................................................................................................................................................................................................................................... 19 0 1 28.00 28 9 0 0 2 3 0 1 0 1 1 4 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..........................................................TATGTCGTCGACCCGGGTACAGTGC.......................................................................................................................................................................................................................................................................... 25 0 1 22.00 22 9 0 0 0 0 0 4 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
...........................................................................TACAGTGCGTTTCGAGGCTGTATGTGC....................................................................................................................................................................................................................................................... 27 0 1 21.00 21 18 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.........................................................................................................................................................................................................................................................................CACTGTCCCCGGGTCGTCAAACA............................................................. 23 0 1 20.00 20 2 5 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
...........................................................................................................................................................................................................................................................................................ATACAATCCCCGGCGAGATT.............................................. 20 3 5 19.40 97 0 0 0 55 0 0 2 0 0 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 5 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
...............................................................................................................................................................................................TCAGGACCGCGATTAGAACGAACGAAA................................................................................................................................... 27 0 1 19.00 19 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 9 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
................................................................................................................................................................................................CAGGACCGCGATTAGAACGAACGAAA................................................................................................................................... 26 0 1 18.00 18 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
...............................................................CGTCGACCCGGGTACAGTGC.......................................................................................................................................................................................................................................................................... 20 0 1 18.00 18 3 2 0 0 1 0 1 1 0 0 0 0 0 1 0 0 0 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
..............................................................TCGTCGACCCGGGTACCGTGC.......................................................................................................................................................................................................................................................................... 21 1 1 16.00 16 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 2 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
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............................................................TGTCGTCGTCCCGTGTACAGTGCG......................................................................................................................................................................................................................................................................... 24 2 1 1.00 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
................................................................................................................................................................TGCAGGTTCAGCAATCCACTTAGT..................................................................................................................................................................... 24 0 1 1.00 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.........................................................................................................................................................................................................GATTAGAACGAACGAAATTTTGCATT.......................................................................................................................... 26 0 1 1.00 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..............................................................TCGTCGACCCGGGTAAAATG........................................................................................................................................................................................................................................................................... 20 2 1 1.00 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..........................................................TATGTCGTCGACCCGGGTACAGTG........................................................................................................................................................................................................................................................................... 24 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
................................................................................................................................................................................CACTTAATTTCGTACTCAGGACCGCGA.................................................................................................................................................. 27 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..................................................................................................................................................................................................................................................TCTTCGCCTACAGCACCGAAACGCACT................................................................................ 27 0 1 1.00 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..............................................................TCGTCGACCCGGGTACAGTAC.......................................................................................................................................................................................................................................................................... 21 1 1 1.00 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.........................................................................................................................................................................................................................................................CTACAGCACCGAAACGCACT................................................................................ 20 0 1 1.00 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.................................................................................................................................................................GCAGGTTCAGCAATCCACTTAGTT.................................................................................................................................................................... 24 0 1 1.00 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.................................................................................................................................................................................................................................................................................................TCCCAGGCGAGATTTAATGTTTTTT................................... 25 0 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
......................................................................................................................................................................TTCAGCAATACACTTAATTTCGTAC.............................................................................................................................................................. 25 2 1 1.00 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..................................................CGAAAGGTTATGTCGTCGACCCGGGT................................................................................................................................................................................................................................................................................. 26 0 1 1.00 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.........................................................................GGTACAGTGCGTTTCGAGTC................................................................................................................................................................................................................................................................ 20 1 1 1.00 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..........................................................................................................................................................................................................................................................................ACTGTCCCCGGGTCGTCAC................................................................ 19 1 1 1.00 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
......................................................................................................................................................................................................CGCGATTAGAACGAACGAAA................................................................................................................................... 20 0 1 1.00 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
...............................TCCGCAGTCCAGTGAAAAGCCAAAGG.................................................................................................................................................................................................................................................................................................... 26 1 1 1.00 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.................................................................................................................................................................GCAGGTTCAGCAATCCACT......................................................................................................................................................................... 19 0 1 1.00 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Anti-sense strand reads

AAACATTCTTGATGGAGGGGGAGGAGGGGTAAGGCGTCAGGTCACTTTTCGCTTTCCAATACAGCAGCTGGGCCCATGTCACGCAAAGCTCCGACATACACGAACAAAATTAGGGGTTAGTATGCGTAAGTCGTTGGATTCCAAAGTAAGCTTCAGTAAGACGTCCAAGTCGTTAGGTGAATCAAAGCATGAGTCCTGGCGCTAATCTTGCTTGCTTTAAAACGTAAACAGTGTAAGTGTAGAGAAGCGGATGTCGTGGCTTTGCGTGACAGGGGCCCAGCAGTTTGTGAGGGTCCGCTCTAAATTACAAAAAAAAAAAAAGAAGGAGTAAATGAATCTTAGGCATTCT
**********************************************************....((...(((.((....(((((((((.(((((((((((((..((((((((((..(((.........................((((..(((((((((.((((...((....(((.(((((.........................))))))))....)).)))).)))))))))...)))).)))..))))..........))))))..))))))))))))).))))))))).)))))..))*********************************************** 		Read size # Mismatch Hit Count Total Norm Total SRR031702
2'-O-methylated small RNAs from r2d2 heterozygous flies
 SRR031701
Total small RNAs from r2d2 heterozygous flies
 GSM609222
ML-DmBG1-C1		 V091
fGS/OSS total  		 V129
ML-DmBG1-c1		 V086
female body, aged
 SRR031703
Total small RNAs from r2d2 homozygous flies
 GSM609224
female, one day
 GSM609238
embryo 14-24hr
 GSM609234
CS  male total RNA  
 SRR029633
total small RNAs from hen1 homozygous flies
 SRR029030
dcr-2 knockdown
 V092
dcr-2[G31R] male total RNA  
 V0662
r2d2 ovary, AGO1-IP, reseq
AGO1		 SRR010953
Aub heterozygotes, oxidized
 V073
mbn2 		GSM628272
ago2[414] ovary total RNA
 GSM609217
MLDmD20c5		 SRR317117
genotype: zuc_hm27/zuc_cd761stage: from 5-6 days old flies
 GSM280087
S2cell (AGO2IP)
AGO2		 SRR014270
Embryo_0-2hrs_rep1_NA_0_2hr_Emb
 GSM609223
male, one day
 SRX247213
FLAG-IP from S2-R+, FLAG-HA-AGO2
AGO2		 GSM467729
Dmel_wt_sRNAseq
 SRR298711
strain: egg1473/ Df(2R)Dll-Mpgenotype/variation: dSETDB1 Mutant
 GSM628271
r2d2[1] ovary total RNA  
 V137
Male aged head
 GSM609225
ML-DmBG3-C2		 V078
Desiccation, female head
 V077
cold, female head
 V093
loqs-KO, 40A; loqs-PB rescue/TM3  male total RNA
 V136
Male aged body
 SRR001664
homozygous_dcr-2_untreated
 SRR317115
genotype: fs(1)yb_72/fs(1)yb_4stage: from 5-6 days old flies
 SRR032096
AGO2 IP dcr2 knockdown
AGO2		 SRR341117
transfected with dsRNA targeting nibblercell type: Schneider 2 (S2) cellsoxidation
 V003
dsDcr-1 (katsutomo RNA)
 V012
Dcr2 male (Katsutomo, whole fly?)
 V015
DreRFHV148h
 V034
ML-DmD16c3 cell		 V036
ML-DmD20c5 cell		 GSE24545
CS ovary total RNA
 SRR001347
ago2_untreated
 SRR031704
2'-O-methylated small RNAs from r2d2 homozygous flies
 SRR298712
strain: bam(delta)86/bam(delta)86genotype/variation: Bam Mutant
 V074
S3		 GSM609232
r2d2[1] ovary total RNA  
 SRR032152
Ago1-associated small RNAs from Oregon R
AGO1		 SRR014269
Embryo_0-2hrs_rep1_LK_0_2hr_Emb
 V079
Oxidation, female head
 V130
ML-DmBG3-c2 		SRR014278
Ovary_rep1_w1118(fem)_x_Har(male)_F1
 V085
CME W2 wing disc		 GSM379065
Zuc Heterozygote
 GSM379066
Zuc Mutant
 GSM467730
Dmel_r2d2_sRNAseq
 SRR010959
Ago3 IP in heterozygotes
AGO3		 SRR031698
2'-O-methylated small RNAs from dcr-2 heterozygous flies
 SRR031699
Total small RNAs from dcr-2 homozygous flies
 SRR298537
strain: vret148-60/vret148-60genotype/variation: Vret Mutant
 SRR317111
genotype: DF(3L)armi-delta1/DF(3L)armi-delta1Stage: from 5-6 days old flies
 GSM609249
ML-DmD21 cell		 V0642
ago2 ovary, AGO1-IP, reseq
AGO1		 V133
Sg4 		SRR001349
heterozygous_dcr-2_untreated
 V145
S2-DRSC		 SRR029608
total small RNAs from hen1 heterozygous flies
 SRR031692
Total small RNAs from Oregon R
 V080
Starvation, female head
 GSM399110
KC-48 #2
 SRR029028
untreated (mock)
 GSM609219
GM2 cell		 GSM609227
CMEW1 Cl.8+ cell		 SRR010954
Aub trans-heterozygotes, oxidized
 SRR031697
Total small RNAs from dcr-2 heterozygous flies
 SRR032093
ago1 knockdown
 SRR097865
Drosophila S2-NP cells
 V008
S2-DRSC		 GSM343832
S2R+ cell		 GSM609230
CS,ovary,AGO1IP
AGO1		 GSM609231
ago2[414], ovary, AGO1IP
AGO1		 SRR001348
ago2_oxidized
 SRR001339
WT_females_non-beta-eliminated
 SRR065802
Piwi-IP_zuc_mut_ovaries
 GSM379059
Piwi Mutant
 GSM609242
s2+48 #2
 SRR014268
Embryo_0-2hrs_rep1_HAR_0_2hr_Emb
 SRR014275
Ovary_rep1_LK_P
 SRR031693
2'-O-methylated small RNAs from Oregon R
 GSM609239
IR- 2-18hr
 V022
ML-DmD32 cell		 GSM609221
1182-4H cell		 V031
GM2 cell		 GSM609233
r2d2[1], ovary, AGO1IP
AGO1		 GSM371638
S2-NP		 GSM609229
embryo 2-6hr
 V139
Cold_female_body
 GSM343287
Drosophila Toll 10b mutant embryos
 GSM385821
OSS_s7		 SRR001338
IR_non-beta-eliminated
 SRR014272
Embryo_0-2hrs_rep1_wK_0_2hr_Emb
 V146
S1 cell		 SRR029031
loqs-ORF knockdown
 SRX247212
AGO1-IP from S2-R+, FLAG-HA-AGO2
AGO1		 GSM272653
KC -48 #1
 SRR341115
transfected with dsRNA targeting nibblercell type: Schneider 2 (S2) cellsoxidation
 GSM385744
OSS_s2		 SRR014274
Ovary_rep1_LK(fem)_x_Har(male)_F1
..............................AAGGCGTCAGGTCACGATC............................................................................................................................................................................................................................................................................................................ 19 3 12 9.08 109 0 0 2 32 72 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0
..............................AAGGCGTCAGGTCACGAT............................................................................................................................................................................................................................................................................................................. 18 2 3 6.67 20 0 0 1 16 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.............................CAAGGCGTCAGGTCACGAT............................................................................................................................................................................................................................................................................................................. 19 3 16 3.31 53 0 0 2 19 27 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
.........................................................................................TCCGACATACACGAGCAAAAT............................................................................................................................................................................................................................................... 21 1 1 2.00 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.....................................................................................................................................TTGGATTCCACAGTACGC...................................................................................................................................................................................................... 18 2 1 2.00 2 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
........TTGATGGAGGGGGACGAAGGT................................................................................................................................................................................................................................................................................................................................ 21 3 4 2.00 8 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..........................GTGTAAGGCGCCAGGTCAGT............................................................................................................................................................................................................................................................................................................... 20 3 7 1.71 12 0 0 0 0 0 0 0 0 0 0 0 12 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
............................GTAAGGCGTCAGGTCACGATC............................................................................................................................................................................................................................................................................................................ 21 3 2 1.50 3 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
................................................................................................................................................................................................................TGCTTGGTTTATCACGTAAAC........................................................................................................................ 21 3 3 1.33 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
................................................................................................................................................................ACGTCCACGTCGGAAGGTGA......................................................................................................................................................................... 20 3 18 1.28 23 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12 0 0 1 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
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..........GATGGAGGGAGAGGAGGGT................................................................................................................................................................................................................................................................................................................................ 19 2 4 0.25 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
........................GGGTTTAAGGCGTCAGGTCAC................................................................................................................................................................................................................................................................................................................ 21 3 4 0.25 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..........GCCGGAGGGGCAGGAGGGGTA.............................................................................................................................................................................................................................................................................................................................. 21 3 4 0.25 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.....................................................................................................................................................................................................................................AGTGTGAGTGTAGAGGAGCGT................................................................................................... 21 3 4 0.25 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
...........ATGGAGTGGTAGGGGGGGTAA............................................................................................................................................................................................................................................................................................................................. 21 3 4 0.25 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.........................................................................................................................................................................................................................................................................GTGAAATGGGCCCAGCAGAT................................................................ 20 3 4 0.25 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
.......................GAGGGGTAAGGAGGCAGGT................................................................................................................................................................................................................................................................................................................... 19 2 4 0.25 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
.............GGAGGGGGAGGAGAGCTCAGG........................................................................................................................................................................................................................................................................................................................... 21 3 4 0.25 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
..............................................................................................................TAGGGGTTAGTAGGCGTACAT.......................................................................................................................................................................................................................... 21 3 4 0.25 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
.......CGGGATGTAGGGGGAGGAGGG................................................................................................................................................................................................................................................................................................................................. 21 3 4 0.25 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
........TGGATGGAGCGGGAGGAGG.................................................................................................................................................................................................................................................................................................................................. 19 2 4 0.25 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
...........................................................................................................................................................................................................................................................TGGCGTGGGTTTGCGTGA................................................................................ 18 2 4 0.25 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
............................................................................................................................................................................................................................................GTGTAGAGGAGGGGAAGTCGT............................................................................................ 21 3 4 0.25 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Re-alignment of all predicted orthologs

Species Coordinate ID Alignment
dm3 chrX:9219679-9220027 - sblock180959 TTTGTAAG--AACTACC--TCCCCCTCCTCCC---------C-ATTCCGCAGTCCAGTGAAAAGCGAAAGGTTAT---GT-CGTCGA--CCCGGGTACAGTGCGTTTCGAGGCTGTATG-TGCTTGTTTTAATCC-CC----AATCATACGCATTCAGCA------ACCTAAGGTTTCATTCGAAGTCATTCT---GCAGGTTCAGCAATCCACTTAGTTTCGTACTCAGGACC--GCGATTAGAACGAACGAAATTTTGCATTTGTCACATTCAC--ATCTCTTC--G--------CCTACAGCACCGAAACGCACTGTCCCCG---GG----------TCGTCAAACACTCCCAGGC----------GAGATTTA-ATG--TT---T---TTT--T-------------TTTTTTCTTCC---TCATTTACTTAGAATCCGTAAGA
droSim2 x:8766597-8766938 - TTTGTAAG--AACTACC--TCC--CTCCTCTC---------C-ATTCCGCAGTCCAGTGAAAAGCGAAAGGTTAT---GT-CTTCGA--CCCGGGGACAGTGCGTTTCGAGGCTGTATG-TGCTTGTTTTAATCC-CC----ATTTATACGCATTCATCA------ACCTAAGGTTTCATTTGAAGTCATTCT---GCAGATTCAGCAATCCACTTAGTTTCTTACTCAGGACC--GCGATTAGAACGAACGAAT--TGGCGTTTGTCACTTTCAC--CTCTCTTC--A--------CCTACAGCGCCGAAACGCACTGTCCCCG---GA----------TTGTCAAACGCTCCCAGGC----------GAGATTTA-ATG--GT---T---TT------------------TTTTTTTTCC---TCATTTACTTAGAATTCGTAAGA
droSec2 scaffold_68:110154-110490 - TTTGTAAG--AACTACC--TCC--CTCCTCTC---------C-ATTCCGCAGTCCAGTGAAAAGCGAAAGGTTAT---GT-CGTCGA--CCCGGGGACAGTGCGTTTCGAGGCTGTATG-TGCTTGTTTTAATCC-CC----ATTCATACGCATTCAGCA------ACCTAAGGTTTCATTTGAAGTCATTCT---GCAGATTCAGCAATCCACTTATTTTCTTACTCAGGACC--GCGACTAAAACAAACGAAA--TGGCGTTTGTCACTTTCAC--ATCTCTTC--G--------CCTACAGCGCCGAAACGCACTGTCCCCA---GG----------TTGTCATACGCTCCCAGGC----------GAGATTTA-ATG--TT-----------------------------TTTTTTCC---TCATTTACTTAGAATTCGTAAGA
droYak3 X:17812825-17813153 - TTTGTAAG--AACTACACCTCC--CTCCTCTC---------C-ATTCCGCAGTCCAGTGA--AGCGAAAGGTTAT---GT-CGTCG---CCC-AGTACAGTGCGTTACGAGGCTATATG-TGCTTGTTGTAA---------------------TACAGC-------ACCTAAGGTTTCATTCGAAGTCATTCT---TTGGGTTCAGCAACCCACATAGTTTCGTACTCAGGACC--ATGATTAGAAC----GAAA--TGGCATTT-GCACCATCAC--ACCGCTGC--G--------CCTACAGCTGCGAAACGCACTGTACTCG---GG----------TCGTCAAACACTCCCAGAC----------GAGATTTA-ATG--TT---T---TTTTTT-------TTTTTTTTCTTGTTTCC---TCATTTACTTAGAATTCGTAAGA
droEre2 scaffold_4690:5899725-5900055 + TTTGTAAG--AACTACC--TCC--CTCCTCTC---------C-ATTCCGCAGTCCAGTGA--AGCGAAGGGTTAC---GT-CGCCG---CCC-AGTACAGTGCGTTGCGAGGCTGTATG-TGCTTGTTGCAATCC-CAGCCTCTTCATGCCCCTTCAGCA------ACCTAAGGTTTCATTCGAAGTCATTC---------------AACCCACATAGTTTCGTGCTCAGGAAAT-GACATTAGAAC----GAAA--TGGCATTT-TCACGTTCAC--ATCTCTTC--G--------CCTACAGCCCCGAAACGCACTGTACCCG---GC----------TCGTCAACTACTCCCAGAC----------GAGATTTA-ATG--GT---T---TTTCCC-------------TTTTTTCCCCC---TCATTTACTTAGAATTCGTAAGA
droEug1 scf7180000409032:737152-737475 + TTTGTAAG--AACTACC--TCC--CTCCTCTC---------C-ATTCCGCAGACCAGTGA--AGCGAAAGGTTTA---CTTTCTGG---CCC-AGTATAGTGCGTTTCGTGGCCGTATA------GCTATAGTCC-TT----ATTCC--TTTATTCAACA------ATCTAAGGTTTCATTCGAAGTCATTCT---ATAAGTTC---AAACCACTTAATTTTTTACTCAGGATA--ATGATTAGAAG----GAAA--TGGCATTT-TCACTTTCAC--AAATCACT--G--------ATTACAGCAACGAAACGCACTGTCCTCCGCCGG----------TTATTAAACGC-CCCAGAT----------GGGAATTATATG--TA---------------------------TTTTTTTTCC---TCATTTAACTAGAATTTGTAAGA
droBia1 scf7180000301760:5192739-5193060 - TTTGTAAG--AACTACC--TCC--CTCCTCTC---------C-ATTCCTCAGTCCAGTGAAGTGCGAAAGGTCAA---AT-CGCGA---CCCGGGTACGGTGCGTTTCGGGTC-GTATG---CCTG---CAATAC-TT----A------TTTATTCAGCA------ACCTAAGGTTTCATTCGAAGTCATTCT---ATATGTTG---AACGCACTTGATTTCTCGCTCAGGATA--TTAATTACCAG----CAAA--CGGCATCC-TCACTTTCAC--CAGTCACC-----------CTTACGGCCCCGAAACGCACCGTCCCCGC-GGGT---------GCGTTACACACTCCCAGTA----------GAGTTTTA--TATATT---T-----------------------TTTCTTCCCA---TAATTTACTTAGAATTCGTAAGA
droTak1 scf7180000414672:184319-184599 + TTTGTAAG--AACTACC--TCC--CTCCCCTT-AACTCCCCTAATTTCTCAGTCCAGTGA--AGCGAAAGGTT--------------------GGGACAGTGCGTTTCGTGGCC--------CTTG-----------------------TCTATTCAGCC------ACCTAAGGTTTCATTCAAAGTCATTCT---ATTAGTTT---AACGCACTTACTTTCTTACTCAGGACA--ATGATTAGCAG----CAAA--TGGCATTT-TCACCTTCACACAAATCA--------------------CCCCGGAACGCACTGTCTCTG------------------------ACTCCCAGTA----------GAGATTTA-T-------------TT--CC-------------TTTCCCCGCCC---TCATTTACTTAGAATTCGTAAGA
droEle1 scf7180000491018:31054-31303 + TTTGTAAGAGAACTACC--TCC--CTCTTATCCGATTCCTCCAGTCCTCCAGTCCAGTGC--AGCGAAA----------T-CGTAGCCCCCC-AGTACAGTGCGTTTCGGGAGCGTAG--------------------------------------AGCAGTCGAGCCCCAAGGTTTCATTCGAAGTCATTCTTCTATTAGTTCAGCCACCCACTTTATTTCTCACTCAGGATAT-TTGATTAACAG----A-------------------TTAAC--AAATCTTCCCC-------CCTTACAGCCCCGAAACGCATTGTCTT-------------------------------------------------------------------------------------------TTTTTTC-------T----TTAG-------AGAA
droRho1 scf7180000780067:115311-115629 - TTTGTAAG--AACTACC--TCC--CTCCTCTC---------C-ATTCCTCAATCCAGTGA--AGCGAAAGGTTTA---GT-CGTAG---CCC-AGTACAGTGCGTTTCGGGGCCTTA--------------------------------TTTATTCAGCA------ACCTAAGGTTTCATTCGAAATCATTCT---ATAAGTTT---AACTCACTTAATTTCATACTCAGGATA--ATGATTAGAAG----GAAA--TGGCATTT-TCACTTTCAC--AAATCTCC--C-------CCTTACAGTCCCGAAACGCACTGTCCCTG---GG-------TCGTCGTCACATACTCCCAAAA----------GAGATTTT-T-------------TTT--TCTCATATTTGTTTTTTTTTGTTCG---TCATTTACTTAGAATTTGTAAGA
droFic1 scf7180000454073:2134513-2134859 - TTTGTAAG--AACTACC--TCC--CTCCTCTC---------C-ATTGCTCGATCCAGTGA--AGCGAAAGGTTATTCGAT-CGCAG---CCC-GGTACAGTGCGTTTCGGAGCTGTAGGGTGTGTG---T-ATCCCTT----A------TTTATTCAGCA------ACCTAAGGTTTCATTCGAAGTCATTCT---ATAAGTTT---AATTCACTTACTTTCTTACTCAGGAGATAATGTTTAGAAG----GAAA--TAGCATTT-GCACTTTCAC--AAATCACC--TCCTACGCTCTTACAGCCCCGAAACGCACTGTCCTCG---GG----------TCGTCACATACTCCCAAAA----------GAGATTTG-GTT--TAGGTTTCTTTT--T-------------TTTTTTGTTCATAATGATTTAATTAGAATTCGTAAGA
droAna3 scaffold_13334:330338-330355 + TA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTAGTAATCGTAAGT
droBip1 scf7180000396431:1209944-1210013 - ATTTATTG--TTTT--------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTTTCGATCAAACA---T--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ATAATATATAGTATATTTT---ATGTATATTTAGTAATCGTAAGT
dp5 XL_group1a:7788495-7788588 + CC---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TACAGCCCTGAAACGCACTGTATATGT-TAGTATGGGGA--CAGCCA---ACGCGCAGTA----------GAAACTT--------------------------------------CTCTGTTA---TAATTTTGTTTTTACATTTAAGA
droPer2 scaffold_15:138776-138874 + CC---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TACAGCCCTGAAACGCACTGTATATGT-TAGTATGGGGA--CAGC--------CATAGAAACGCGCAGTAGCAAC--------------------------------------TTCTCTGTTA---TAATTTTGTTTGTACATTTAAGA
droMoj3 scaffold_6473:15804337-15804372 + TGTTT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TT--TT-------------TTTTCCTTTTC---TTTTTTATATAAAATC-------

Re-alignment of all predicted orthologs with sRNA reads

Species Read alignment
dm3
TTTGTAAG--AACTACC--TCCCCCTCCTCCC---------C-ATTCCGCAGTCCAGTGAAAAGCGAAAGGTTAT---GT-CGTCGA--CCCGGGTACAGTGCGTTTCGAGGCTGTATG-TGCTTGTTTTAATCC-CC----AATCATACGCATTCAGCA------ACCTAAGGTTTCATTCGAAGTCATTCT---GCAGGTTCAGCAATCCACTTAGTTTCGTACTCAGGACC--GCGATTAGAACGAACGAAATTTTGCATTTGTCACATTCAC--ATCTCTTC--G--------CCTACAGCACCGAAACGCACTGTCCCCG---GG----------TCGTCAAACACTCCCAGGC----------GAGATTTA-ATG--TT---T---TTT--T-------------TTTTTTCTTCC---TCATTTACTTAGAATCCGTAAGASizeHit CountTotal NormTotalGSM154618
Ovary		GSM154620
Ovary		GSM154621
Ovary		GSM154622
Ovary		GSM180328
Head		GSM180329
Mixed-body		GSM180330
Embryo		GSM180331
Embryo		GSM180332
Embryo		GSM180333
Embryo		GSM180334
Larvae		GSM180335
Disc		GSM180336
Pupae		GSM180337
S2		GSM231091
Ovary		GSM239041
Head		GSM239050
Head		GSM239051
S2		GSM239052
S2		GSM246084
Head		GSM266765
S2		GSM280082
Ovary		GSM280083
Ovary		GSM280084
Ovary		GSM280085
Testis		GSM280086
Ovary		GSM280087
S2-NP		GSM280088
S2-NP		GSM312995
Head		GSM313160
Head		GSM313161
Head		GSM313162
Head		GSM313163
Head		GSM313164
Head		GSM313165
Head		GSM343287
Embryo		GSM379050
Ovary		GSM379051
Ovary		GSM379052
Ovary		GSM379053
Ovary		GSM379054
Ovary		GSM379055
Ovary		GSM379056
Ovary		GSM379057
Ovary		GSM379058
Ovary		GSM379059
Ovary		GSM379060
Ovary		GSM379061
Ovary		GSM379062
Ovary		GSM379063
Ovary		GSM379064
Ovary		GSM379065
Ovary		GSM379066
Ovary		GSM379067
Ovary		GSM424739
S2		GSM424740
S2		GSM424741
S2		GSM467729
Female-body		GSM467730
Female-body		GSM467731
Female-body		M002
CS		M003
Male-body		M004
Male-body		M005
ML-DmD32		M006
ML-DmD32		M029
OSC		M030
OSC		M031
OSC		M032
S2-R+		M033
S2-R+		M034
S2-R+		M035
S2-R+		M036
S2-R+		M037
S2-R+		M038
S2-R+		M039
S2-R+		M048
S2-R+		OSS2
OSS		OSS6
OSS		OSS7
OSS		OSS8
OSS		S1
Head		S10
Embryo		S11
Embryo		S12
Embryo		S13
Embryo		S14
Embryo		S15
S2-GMR		S16
Kc		S17
Larvae		S18
Larvae		S19
Pupae		S2
Head		S20
Pupae		S21
Disc		S22
Head		S23
Female-body		S24
Male-body		S25
Larvae		S26
Larvae		S27
Pupae		S28
Pupae		S29
Head		S3
Male-body		S30
S2-GMR		S31
S2		S32
S2		S33
Kc		S34
Kc		S35
Kc		S4
Female-body		S5
Disc		S6
Embryo		S7
Embryo		S8
S2		S9
Embryo		SRR001337
Head		SRR001338
Head		SRR001339
Head		SRR001340
Head		SRR001341
Head		SRR001342
Head		SRR001343
Head		SRR001344
Head		SRR001345
Head		SRR001346
Head		SRR001347
Head		SRR001348
Head		SRR001349
Head		SRR001664
Head		SRR010951
Ovary		SRR010952
Ovary		SRR010953
Ovary		SRR010954
Ovary		SRR010955
Ovary		SRR010956
Ovary		SRR010957
Ovary		SRR010958
Ovary		SRR010959
Ovary		SRR010960
Ovary		SRR014268
Embryo		SRR014269
Embryo		SRR014270
Embryo		SRR014271
Embryo		SRR014272
Embryo		SRR014273
Ovary		SRR014274
Ovary		SRR014275
Ovary		SRR014276
Ovary		SRR014277
Ovary		SRR014278
Ovary		SRR014279
Ovary		SRR014280
Ovary		SRR014281
Ovary		SRR014282
Ovary		SRR023197
S2		SRR023399
S2		SRR023400
S2		SRR023402
S2		SRR023407
S2		SRR029028
S2		SRR029029
S2		SRR029030
S2		SRR029031
S2		SRR029032
S2		SRR029033
S2		SRR029608
Head		SRR029633
Head		SRR030145
gb3		SRR031692
Head		SRR031693
Head		SRR031694
Head		SRR031696
Head		SRR031697
Head		SRR031698
Head		SRR031699
Head		SRR031700
Head		SRR031701
Head		SRR031702
Head		SRR031703
Head		SRR031704
Head		SRR032092
S2		SRR032093
S2		SRR032094
S2		SRR032095
S2-NP		SRR032096
S2-NP		SRR032152
Head		SRR060643
Testis		SRR060644
Ovary		SRR060645
Testis		SRR060646
Ovary		SRR060647
Ovary		SRR060648
Ovary		SRR060649
Ovary		SRR060650
Testis		SRR060651
Ovary		SRR060652
Testis		SRR060653
Ovary		SRR065151
Ovary		SRR065152
Ovary		SRR065153
Ovary		SRR065154
Ovary		SRR065155
Ovary		SRR065800
Ovary		SRR065801
Ovary		SRR065802
Ovary		SRR065803
Ovary		SRR065804
Ovary		SRR065805
Ovary		SRR065806
Ovary		SRR065807
Ovary		SRR097865
S2-NP		SRR097866
S2-NP		SRR097867
S2-NP		SRR298536
Ovary		SRR298537
Ovary		SRR298711
Ovary		SRR298712
Ovary		SRR317110
Ovary		SRR317111
Ovary		SRR317112
Ovary		SRR317113
Ovary		SRR317114
Ovary		SRR317115
Ovary		SRR317116
Ovary		SRR317117
Ovary		SRR341115
S2		SRR341116
S2		SRR341117
S2		SRR341118
S2		SRR351332
Mixed-body		SRR351333
Mixed-body		T004
OSC		T005
CME-L1		V001
Embryo		V002
Embryo		V003
S2		V006
Female-body		V007
Head		V008
S2		V009
CME-L1		V010
ML-DmD20-C5		V011
Sg4		V012
Male-body		V013
Head		V014
DTT		V015
DreRFHV		V018
Kc167		V019
GM2		V020
S2-R+		V021
ML-DmD21		V022
ML-DmD32		V023
Head		V024
Kc167		V025
S2-R+		V026
1182-2H		V027
ML-DmD21		V028
CME-W1-Cl.8+		V029
ML-DmD9		V030
ML-DmD8		V031
GM2		V032
S1		V033
CME-W1-Cl.8+		V034
ML-DmD16-C3		V035
ML-DmD32		V036
ML-DmD20-C5		V037
S2		V038
S2		V063
Ovary		V0632
Ovary		V064
Ovary		V0642
Ovary		V065
Ovary		V0652
Ovary		V066
Ovary		V0662
Ovary		V067
S2-NP		V073
mbn2		V074
S3		V075
ML-DmBG1-C1		V076
ML-DmBG3-C2		V077
Head		V078
Head		V079
Head		V080
Head		V081
Embryo		V082
Embryo		V083
Male-body		V084
Female-body		V085
CME-W2		V086
Female-body		V088
Ovary		V0882
Ovary		V089
Ovary		V0892
Ovary		V090
Male-body		V091
fGS/OSS		V092
Male-body		V093
Male-body		V094
S2-R+		V095
S2-R+		V096
Ovary		V097
Ovary		V098
Ovary		V125
ML-DmD9		V126
CME-L1		V127
G2		V128
S3		V129
ML-DmBG1-C1		V130
ML-DmBG3-C2		V131
ML-DmD16-C3		V132
ML-DmD32		V133
Sg4		V134
ML-DmD8		V135
CME-W2		V136
Male-body		V137
Head		V138
Male-body		V139
Female-body		V140
Female-body		V141
Female-body		V142
Female-body		V144
OSC		V145
S2		V146
S1		V147
1182-2H		V148
mbn2
...............................................................................T-CGTCGA--CCCGGGTACAGTGC.........................................................................................................................................................................................................................................................................................................................................................2112578.0025780000000000000000000000002071400000003353406542101146410000000000000000000000000000000003000000130200000052000000000000000000000000000000000000000105693940711400000143312223341000126001000501392300905222545002012800000010101002421151547919000001001150000000769422002302191031600481204001001501022500211060402261621170001127143116300252030023155810213110254458343245452695343617348
...............................................................................T-CGTCGA--CCCGGGTACAGTGCG........................................................................................................................................................................................................................................................................................................................................................221160.00160000000000000000000000000001000000000000000000000000000000000000000000000000000000000000200000000010000600050000000000000000000000000000000000000000011500000000060003000000000000000000000010000000000000000002000034103210000000000200000002000000020100000000010200000010011212000228300201100000001011100200100291001000052100211010123
...............................................................................T-CGTCGA--CCCGGGTACAGTG..........................................................................................................................................................................................................................................................................................................................................................201128.0012800000000000000000000000000001000000101100011000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000010040302000002050500000010000001000500102240000000000000000000000010310000000000000000000000000000000000000000000000000000001000005100200100000002040000100000010000000112111114200010
......................................................................................A--CCCGGGTACAGTGCGTTTCG...................................................................................................................................................................................................................................................................................................................................................211109.0010900000000000000000000000000000000000000000000000000000000000000000000000000000000000000010000000000000000000000000000000000000000000000000000000000000110030000000000001000000000000000000000000000100200000000100000000001000000000000000000000100000000010000000000000000000031000021100000000000002013110020040004000101000100110482005
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....................................................................................................................................................................................................GCAGGTTCAGCAATCCACT.........................................................................................................................................................................................................................................1911.001000000000000000000000000000000000000000000000000000000000001000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
droSim2
TTTGTAAG--AACTACC--TCC--CTCCTCTC---------C-ATTCCGCAGTCCAGTGAAAAGCGAAAGGTTAT---GT-CTTCGA--CCCGGGGACAGTGCGTTTCGAGGCTGTATG-TGCTTGTTTTAATCC-CC----ATTTATACGCATTCATCA------ACCTAAGGTTTCATTTGAAGTCATTCT---GCAGATTCAGCAATCCACTTAGTTTCTTACTCAGGACC--GCGATTAGAACGAACGAAT--TGGCGTTTGTCACTTTCAC--CTCTCTTC--A--------CCTACAGCGCCGAAACGCACTGTCCCCG---GA----------TTGTCAAACGCTCCCAGGC----------GAGATTTA-ATG--GT---T---TT------------------TTTTTTTTCC---TCATTTACTTAGAATTCGTAAGASizeHit CountTotal NormTotalM023
Head		M024
Male-body		M025
Embryo		M053
Female-body		O001
Testis		O002
Head		SRR553485
Ovary		SRR553486
Ovary		SRR553487
Ovary		SRR553488
Ovary		SRR618934
Ovary		V044
Embryo		SRR902008
Ovary		SRR902009
Testis		SRR1275487
Male larvae		SRR1275485
Male prepupae		SRR1275483
Male prepupae
..................................................................................................................................................................................................................................TCAGGACC--GCGATTAGAACGAACGAAT--...............................................................................................................................................................................................2716.00601000013010000000
..............................................................................................................................................................................................................................TTACTCAGGACC--GCGATTAGAACGAACGAA..................................................................................................................................................................................................3014.00400000020000002000
...................................................................................................................................................................................................................................................................................................................................................................................................................................TCC---TCATTTACTTAGAATTCGTAAGA2614.00401000020010000000
...............................................................................................................................................................................................................................TACTCAGGACC--GCGATTAGAACGAACGAA..................................................................................................................................................................................................2914.00400000012000001000
.....................................................................................................................................................................................TGAAGTCATTCT---GCAGATTCAGCAATC.............................................................................................................................................................................................................................................2713.00300000000000003000
...............................................................................................................................................................................................................................TACTCAGGACC--GCGATTAGAACGAA......................................................................................................................................................................................................2513.00300000003000000000
....................................................................................CGA--CCCGGGGACAGTGCGTT......................................................................................................................................................................................................................................................................................................................................................2013.00300000000000003000
...............................................................................................................................................................................................................................TACTCAGGACC--GCGATTAGAACGAACGA...................................................................................................................................................................................................2813.00300000003000000000
.................................................................................................................................................................................................................................................................................................................................CCCG---GA----------TTGTCAAACGCTCCCAGGC----------GAGA...........................................................................2913.00300000003000000000
.............................................................................................................................................................................................................................................................................................................................TGTCCCCG---GA----------TTGTCAAACGCTCCCAGG..........................................................................................2812.00200100001000000000
...............................................................................................GACAGTGCGTTTCGAGGCTGT............................................................................................................................................................................................................................................................................................................................................2112.00200000000000002000
...............................................................................................GACAGTGCGTTTCGAGGCTG.............................................................................................................................................................................................................................................................................................................................................2012.00200000000000002000
..TGTAAG--AACTACC--TCC--CTCCTCTC---------C-A....................................................................................................................................................................................................................................................................................................................................................................................................................2612.00200000011000000000
..................................................................................................................................................................................................................................TCAGGACC--GCGATTAGAACGAACGA...................................................................................................................................................................................................2512.00200000020000000000
...............................................................................................GACAGTGCGTTTCGAGGCTGTAT..........................................................................................................................................................................................................................................................................................................................................2312.00200000000000002000
............................................................................................................................................................................................................................TCTTACTCAGGACC--GCGATTAGAACGAAC.....................................................................................................................................................................................................2912.00200000002000000000
.....................................................................................................................................................................................TGAAGTCATTCT---GCAGATTCAGCAA...............................................................................................................................................................................................................................................2512.00200000000000002000
.......................................................................................................................................................................................................................................ACC--GCGATTAGAACGAACGAAT--TGGC...........................................................................................................................................................................................2612.00220000000000000000
..................................................................................................AGTGCGTTTCGAGGCTGTATG-........................................................................................................................................................................................................................................................................................................................................2111.00100000010000000000
.................................................................................................CAGTGCGTTTCGAGGCTGTAT..........................................................................................................................................................................................................................................................................................................................................2111.00100000000000001000
.................................................................................................................................................................................................................................CTCAGGACC--GCGATTAGAAC.........................................................................................................................................................................................................2011.00100100000000000000
.........................................................................................................................................................................................................................................................................................TCTTC--A--------CCTACAGCGCCGAAACGCACAA.................................................................................................................................2811.00100000000000001000
..........................................................................................................................................................................................................................................................................................................................................................................................................................................CATTTACTTAGAATTCGTAA..2011.00100000001000000000
...................................................................................................................................................................................TTTGAAGTCATTCT---GCAGATTCAGCA................................................................................................................................................................................................................................................2611.00100000000000001000
.................................................................GAAAGGTTAT---GT-CTTCGA--CCCGGGGA...............................................................................................................................................................................................................................................................................................................................................................2611.00100000001000000000
...................................................................................TCGA--CCCGGGGACAGTGCGTTTCGAGG................................................................................................................................................................................................................................................................................................................................................2711.00100000001000000000
...................................................................................................................................................................................................................................................................................................................................................................................................................................TCC---TCATTTACTTAGAATTCGTA...2311.00100000000100000000
.................................................................................................CAGTGCGTTTCGAGGCTGTA...........................................................................................................................................................................................................................................................................................................................................2011.00100000000000001000
....................................................................................................................................................................................................................................................AACGAACGAAT--TGGCGTTTGT.....................................................................................................................................................................................2111.00100000000001000000
...............................................................................................................................................................................................................................TACTCAGGACC--GCGATTAGAACGAAC.....................................................................................................................................................................................................2611.00100000000001000000
.................................................................................................................................................................................CATTTGAAGTCATTCT---GCAGATTCAGC.................................................................................................................................................................................................................................................2711.00100100000000000000
....................................................................................CGA--CCCGGGGACAGTGCGTTTT....................................................................................................................................................................................................................................................................................................................................................2211.00100000000000001000
...............................................................................................GACAGTGCGTTTCGAGGCTGTA...........................................................................................................................................................................................................................................................................................................................................2211.00100000000000001000
.................................................................................................................................................................................................................................CTCAGGACC--GCGATTAGAACGAACGA...................................................................................................................................................................................................2611.00101000000000000000
..............................................................................................GGACAGTGCGTTTCGAGGCTG.............................................................................................................................................................................................................................................................................................................................................2111.00100000000000001000
...................................................................................................GTGCGTTTCGAGGCTGTATG-TGCT....................................................................................................................................................................................................................................................................................................................................2411.00110000000000000000
.......................................................................................................................................................................................................................................ACC--GCGATTAGAACGAACGAAT--TGGCG..........................................................................................................................................................................................2711.00110000000000000000
....................................................................................................TGCGTTTCGAGGCTGTATG-TGC.....................................................................................................................................................................................................................................................................................................................................2211.00100000001000000000
................................................................................................................................................................................................................................ACTCAGGACC--GCGATTAGAAC.........................................................................................................................................................................................................2111.00101000000000000000
...............................................................................................GACAGTGCGTTTCGAGGCTGA............................................................................................................................................................................................................................................................................................................................................2111.00100000000000001000
...................................................................................................................................................................................TTTGAAGTCATTCT---GCAGATTCAGCAAT..............................................................................................................................................................................................................................................2811.00100000000000010000
..........................................................................................................................................................................................TCATTCT---GCAGATTCAGCAATCCACT.........................................................................................................................................................................................................................................2611.00100000000000001000
.........................................................................................................TTCGAGGCTGTATG-TGCT....................................................................................................................................................................................................................................................................................................................................1811.00101000000000000000
...................................................................................................................................................................................TTTGAAGTCATTCT---GCAGATTCAGCAA...............................................................................................................................................................................................................................................2711.00100000000000001000
....................................................................................CGA--CCCGGGGACAGTGCGTTT.....................................................................................................................................................................................................................................................................................................................................................2111.00100000000000001000
..................................................................................................................................................................................................................................................................................................................................................................................................................................TTCC---TCATTTACTTAGAATTCGTAAGA2711.00100000010000000000
...................................................................................................GTGCGTTTCGAGGCTGTATG-........................................................................................................................................................................................................................................................................................................................................2011.00100000000000001000
.............................................................................................................................................................................................................................CTTACTCAGGACC--GCGATTAGAAC.........................................................................................................................................................................................................2411.00101000000000000000
TTTGTAAG--AACTACC--TCC--CTCCT...................................................................................................................................................................................................................................................................................................................................................................................................................................2311.00100000001000000000
.....................................................................................................................................................................................TGAAGTCATTCT---GCAGATTCAGCAAT..............................................................................................................................................................................................................................................2611.00100000000000001000
....................................................................................................................................................................................................................................AGGACC--GCGATTAGAACGAACGAAT--...............................................................................................................................................................................................2511.00100000001000000000
..................................................................................................AGTGCGTTTCGAGGCTGTAT..........................................................................................................................................................................................................................................................................................................................................2011.00100000000000001000
....................................................................................................................................................................................TTGAAGTCATTCT---GCAGATTCAGCAA...............................................................................................................................................................................................................................................2611.00100000010000000000
...................................................................................TCGA--CCCGGGGACAGTGCGTT......................................................................................................................................................................................................................................................................................................................................................2111.00100000000000001000
.................................................................................................................................................................................CATTTGAAGTCATTCT---GCAGATTCA...................................................................................................................................................................................................................................................2511.00100010000000000000
........................................................................TAT---GT-CTTCGA--CCCGGGGACAGTGCGT.......................................................................................................................................................................................................................................................................................................................................................2711.00100000000001000000
....................................................................................CGA--CCCGGGGACAGTGCGTTTTT...................................................................................................................................................................................................................................................................................................................................................2311.00100000000000001000
.............................................................................................................................................................................................................................................................................................................................TGTCCCCG---GA----------TTGTCAAACGCTCCCAC...........................................................................................2711.00101000000000000000
............................................................................................................................................................................................................................TCTTACTCAGGACC--GCGATTAGAACGAA......................................................................................................................................................................................................2811.00100000000100000000
...................................................................................................................................................................................................................................CAGGACC--GCGATTAGAACGAACGAAT--...............................................................................................................................................................................................2611.00100000001000000000
....................................................................................................TGCGTTTCGAGGCTGTATG-T.......................................................................................................................................................................................................................................................................................................................................2011.00100000000000001000
...........................................................................................................................................................................................................................................................GAAT--TGGCGTTTGTT....................................................................................................................................................................................15200.05100000000001000000
droSec2
TTTGTAAG--AACTACC--TCC--CTCCTCTC---------C-ATTCCGCAGTCCAGTGAAAAGCGAAAGGTTAT---GT-CGTCGA--CCCGGGGACAGTGCGTTTCGAGGCTGTATG-TGCTTGTTTTAATCC-CC----ATTCATACGCATTCAGCA------ACCTAAGGTTTCATTTGAAGTCATTCT---GCAGATTCAGCAATCCACTTATTTTCTTACTCAGGACC--GCGACTAAAACAAACGAAA--TGGCGTTTGTCACTTTCAC--ATCTCTTC--G--------CCTACAGCGCCGAAACGCACTGTCCCCA---GG----------TTGTCATACGCTCCCAGGC----------GAGATTTA-ATG--TT-----------------------------TTTTTTCC---TCATTTACTTAGAATTCGTAAGASizeHit CountTotal NormTotalM054
Female-body		V113
Male-body		V114
Embryo		V115
Head
.................................................................................................................................................................................................................................................................................................................GCCGAAACGCACTGTCCCCC---........................................................................................................................2011.0011000
....................................................................................................................................................................................................................................................................................................................................................TTGTCATACGCTCCCAGGC----------GAGATT.........................................................................2511.0011000
.........................................................................................CCCGGGGACAGTGCGTTTCGAGGC...............................................................................................................................................................................................................................................................................................................................................2411.0010010
......................................................................................................................................................................................................................................................................TTTGTCACTTTCAC--ATCTCTTC--G--------CC.....................................................................................................................................................2511.0011000
..........................................................................................................................................................................................................................................................................................................CTACAGCGCCGAAACGCACT..................................................................................................................................2011.0010010
.........................................................................................CCCGGGGACAGTGCGTTTCGAGG................................................................................................................................................................................................................................................................................................................................................2311.0010100
...............................................................................................................................................................................TTCATTTGAAGTCATTCT---GCAG........................................................................................................................................................................................................................................................2211.0011000
....................................................................................................TGCGTTTCGAGGCTGTATG-TGC.....................................................................................................................................................................................................................................................................................................................................2211.0010001
...........................................................................................................................................................................AGGTTTCATTTGAAGTCATTCT---GCA.........................................................................................................................................................................................................................................................2511.0011000
..............................................................................................................................................................................................................................................GACTAAAACAAACGAAA--TGGCGTT........................................................................................................................................................................................2411.0010010
.........................................................................AT---GT-CGTCGA--CCCGGGGACAGTGCG........................................................................................................................................................................................................................................................................................................................................................2511.0010001
................................................................................................ACAGTGCGTTTCGAGGCTGT............................................................................................................................................................................................................................................................................................................................................2011.0010010
droYak3
TTTGTAAG--AACTACACCTCC--CTCCTCTC---------C-ATTCCGCAGTCCAGTGA--AGCGAAAGGTTAT---GT-CGTCG---CCC-AGTACAGTGCGTTACGAGGCTATATG-TGCTTGTTGTAA---------------------TACAGC-------ACCTAAGGTTTCATTCGAAGTCATTCT---TTGGGTTCAGCAACCCACATAGTTTCGTACTCAGGACC--ATGATTAGAAC----GAAA--TGGCATTT-GCACCATCAC--ACCGCTGC--G--------CCTACAGCTGCGAAACGCACTGTACTCG---GG----------TCGTCAAACACTCCCAGAC----------GAGATTTA-ATG--TT---T---TTTTTT-------TTTTTTTTCTTGTTTCC---TCATTTACTTAGAATTCGTAAGASizeHit CountTotal NormTotalM026
Head		M043
Female-body		M056
Embryo		V046
Embryo		V052
Head		V058
Head		V120
Male-body		SRR1275488
Male larvae		SRR1275486
Male prepupae		SRR1275484
Male prepupae
TTTGTAAG--AACTACACCTCC--CTCCTCTC---------.......................................................................................................................................................................................................................................................................................................................................................................................................................2811.0010010000000
...................................................................................................................................................................................................................................CAGGACC--ATGATTAGAAC----GA...................................................................................................................................................................................................2011.0011000000000
TTTGTAAG--AACTACACCTCC--CTCCTCTC---------C-.....................................................................................................................................................................................................................................................................................................................................................................................................................2911.0011000000000
.......................................................................................................................................................................CCTAAGGTTTCATTCGAAGTC....................................................................................................................................................................................................................................................................2111.0010100000000
...................................................................................................................................................................................................................................CAGGACC--ATGATTAGAAC----GG...................................................................................................................................................................................................2011.0011000000000
...................................................................................................................................A---------------------TACAGC-------ACCTAAGGTTTCATTCGAA.......................................................................................................................................................................................................................................................................2611.0010100000000
.......................................................................................................................................................................................................................................................................................................................ACGCACTGTACTCG---GG----------TC..........................................................................................................1811.0010010000000
...................................................................................................................................A---------------------TACAGC-------ACCTAAGGTTTC..............................................................................................................................................................................................................................................................................1911.0010001000000
...........................................................................................................................................................................................................................................................................................................................................................................................TG--TT---T---TTTTTT-------TTTTTTTTC..................................2070.1410010000000
droEre2
TTTGTAAG--AACTACC--TCC--CTCCTCTC---------C-ATTCCGCAGTCCAGTGA--AGCGAAGGGTTAC---GT-CGCCG---CCC-AGTACAGTGCGTTGCGAGGCTGTATG-TGCTTGTTGCAATCC-CAGCCTCTTCATGCCCCTTCAGCA------ACCTAAGGTTTCATTCGAAGTCATTC---------------AACCCACATAGTTTCGTGCTCAGGAAAT-GACATTAGAAC----GAAA--TGGCATTT-TCACGTTCAC--ATCTCTTC--G--------CCTACAGCCCCGAAACGCACTGTACCCG---GC----------TCGTCAACTACTCCCAGAC----------GAGATTTA-ATG--GT---T---TTTCCC-------------TTTTTTCCCCC---TCATTTACTTAGAATTCGTAAGASizeHit CountTotal NormTotalM055
Female-body		M057
Embryo		V060
Head		V040
Embryo		V107
Male-body		V108
Head
........................................................................TAC---GT-CGCCG---CCC-AGTACAGTGCGT.......................................................................................................................................................................................................................................................................................................................................................2513.003300000
.........................................................................................CCC-AGTACAGTGCGTTGCGAGGCTG.............................................................................................................................................................................................................................................................................................................................................2511.001100000
.......................................................................................................................................................................................................................................................................TT-TCACGTTCAC--ATCTCTTC--G--------CCTACAG................................................................................................................................................2811.001100000
........................................................................TAC---GT-CGCCG---CCC-AGTACAGTGCGTT......................................................................................................................................................................................................................................................................................................................................................2611.001100000
.............TACC--TCC--CTCCTCTC---------C-ATTCCGCAG............................................................................................................................................................................................................................................................................................................................................................................................................2511.001100000
........................................................................................................................................................................................................................................................................................................................................................CAACTACTCCCAGAC----------GAGATTT........................................................................2211.001100000
................................................................................................................................................................................................................................................TTAGAAC----GAAA--TGGCATTT-TCACGT................................................................................................................................................................................2511.001100000
........................................................................................................................................CAGCCTCTTCATGCCCCTTC....................................................................................................................................................................................................................................................................................................2011.001100000
.......................................................................................................................................................................................................................................................................................................................................................TCAACTACTCCCAGAC----------GAGATTTA-ATG--G................................................................2811.001100000
..................................................................................................AGTGCGTTGCGAGGCTGTATG-........................................................................................................................................................................................................................................................................................................................................2111.001000010
...............................C---------C-ATTCCGCAGTCCAGTGA--AGCGAAGG..........................................................................................................................................................................................................................................................................................................................................................................................2711.001000001
................................................................................................................................................................................................................................................................................................................................................................................................................................TCCCCC---TCATTTACTTAGAATTCGTA...2611.001100000
..........................................................................................................................................................TCAGCA------ACCTAAGGTTTCATTCGAAG......................................................................................................................................................................................................................................................................2611.001100000
...............................................................................................................................................................................TTCATTCGAAGTCATTC---------------AACCCACA.........................................................................................................................................................................................................................................2511.001100000
......................................................................................................................G-TGCTTGTTGCAATCC-CAGCCC..................................................................................................................................................................................................................................................................................................................2211.001010000
..........................................................................................................................................................................................TCATTC---------------AACCCACATAGTTTCGTGCT.............................................................................................................................................................................................................................2611.001100000
.....................................................CCAGTGA--AGCGAAGGGTTAC---GT-CGCCG---C......................................................................................................................................................................................................................................................................................................................................................................2811.001100000
.........................................................................................................................................................TTCAGCA------ACCTAAGGTTTCATTCGAAG......................................................................................................................................................................................................................................................................2711.001100000
..TGTAAG--AACTACC--TCC--CTCCTCTC---------C-AT...................................................................................................................................................................................................................................................................................................................................................................................................................2711.001100000
...............................C---------C-ATTCCGCAGTCCAGTGA--AGCGAAG...........................................................................................................................................................................................................................................................................................................................................................................................2611.001100000
...........................................................................................................................................................................................................................................................................................................................................................................................................................TTTTTTCCCCC---TCATTTACTTAGAAT........2611.001100000
.......................................................................TTAC---GT-CGCCG---CCC-AGTACAGTGCGT.......................................................................................................................................................................................................................................................................................................................................................2611.001100000
.............................................TCCGCAGTCCAGTGA--AGCGAAGGGT........................................................................................................................................................................................................................................................................................................................................................................................2511.001100000
......................................................................................................................................................................................GAAGTCATTC---------------AACCCACATAGTTTCG.................................................................................................................................................................................................................................2611.001100000
...........................................................................................................................................................................................................................................................................................................................................................................................TG--GT---T---TTTCCC-------------TTTTTTCCCCC---TCA....................2511.001100000
................................................................................................ACAGTGCGTTGCGAGGCT..............................................................................................................................................................................................................................................................................................................................................1811.001000001
...................................................................................................................................ATCC-CAGCCTCTTCATGCCCCTTCAGC.................................................................................................................................................................................................................................................................................................2711.001000001
droEug1
TTTGTAAG--AACTACC--TCC--CTCCTCTC---------C-ATTCCGCAGACCAGTGA--AGCGAAAGGTTTA---CTTTCTGG---CCC-AGTATAGTGCGTTTCGTGGCCGTATA------GCTATAGTCC-TT----ATTCC--TTTATTCAACA------ATCTAAGGTTTCATTCGAAGTCATTCT---ATAAGTTC---AAACCACTTAATTTTTTACTCAGGATA--ATGATTAGAAG----GAAA--TGGCATTT-TCACTTTCAC--AAATCACT--G--------ATTACAGCAACGAAACGCACTGTCCTCCGCCGG----------TTATTAAACGC-CCCAGAT----------GGGAATTATATG--TA---------------------------TTTTTTTTCC---TCATTTAACTAGAATTTGTAAGASizeHit CountTotal NormTotal
No Reads
droBia1
TTTGTAAG--AACTACC--TCC--CTCCTCTC---------C-ATTCCTCAGTCCAGTGAAGTGCGAAAGGTCAA---AT-CGCGA---CCCGGGTACGGTGCGTTTCGGGTC-GTATG---CCTG---CAATAC-TT----A------TTTATTCAGCA------ACCTAAGGTTTCATTCGAAGTCATTCT---ATATGTTG---AACGCACTTGATTTCTCGCTCAGGATA--TTAATTACCAG----CAAA--CGGCATCC-TCACTTTCAC--CAGTCACC-----------CTTACGGCCCCGAAACGCACCGTCCCCGC-GGGT---------GCGTTACACACTCCCAGTA----------GAGTTTTA--TATATT---T-----------------------TTTCTTCCCA---TAATTTACTTAGAATTCGTAAGASizeHit CountTotal NormTotal
No Reads
droTak1
TTTGTAAG--AACTACC--TCC--CTCCCCTT-AACTCCCCTAATTTCTCAGTCCAGTGA--AGCGAAAGGTT--------------------GGGACAGTGCGTTTCGTGGCC--------CTTG-----------------------TCTATTCAGCC------ACCTAAGGTTTCATTCAAAGTCATTCT---ATTAGTTT---AACGCACTTACTTTCTTACTCAGGACA--ATGATTAGCAG----CAAA--TGGCATTT-TCACCTTCACACAAATCA--------------------CCCCGGAACGCACTGTCTCTG------------------------ACTCCCAGTA----------GAGATTTA-T-------------TT--CC-------------TTTCCCCGCCC---TCATTTACTTAGAATTCGTAAGASizeHit CountTotal NormTotal
No Reads
droEle1
TTTGTAAGAGAACTACC--TCC--CTCTTATCCGATTCCTCCAGTCCTCCAGTCCAGTGC--AGCGAAA----------T-CGTAGCCCCCC-AGTACAGTGCGTTTCGGGAGCGTAG--------------------------------------AGCAGTCGAGCCCCAAGGTTTCATTCGAAGTCATTCTTCTATTAGTTCAGCCACCCACTTTATTTCTCACTCAGGATAT-TTGATTAACAG----A-------------------TTAAC--AAATCTTCCCC-------CCTTACAGCCCCGAAACGCATTGTCTT-------------------------------------------------------------------------------------------TTTTTTC-------T----TTAG-------AGAASizeHit CountTotal NormTotal
No Reads
droRho1
TTTGTAAG--AACTACC--TCC--CTCCTCTC---------C-ATTCCTCAATCCAGTGA--AGCGAAAGGTTTA---GT-CGTAG---CCC-AGTACAGTGCGTTTCGGGGCCTTA--------------------------------TTTATTCAGCA------ACCTAAGGTTTCATTCGAAATCATTCT---ATAAGTTT---AACTCACTTAATTTCATACTCAGGATA--ATGATTAGAAG----GAAA--TGGCATTT-TCACTTTCAC--AAATCTCC--C-------CCTTACAGTCCCGAAACGCACTGTCCCTG---GG-------TCGTCGTCACATACTCCCAAAA----------GAGATTTT-T-------------TTT--TCTCATATTTGTTTTTTTTTGTTCG---TCATTTACTTAGAATTTGTAAGASizeHit CountTotal NormTotal
No Reads
droFic1
TTTGTAAG--AACTACC--TCC--CTCCTCTC---------C-ATTGCTCGATCCAGTGA--AGCGAAAGGTTATTCGAT-CGCAG---CCC-GGTACAGTGCGTTTCGGAGCTGTAGGGTGTGTG---T-ATCCCTT----A------TTTATTCAGCA------ACCTAAGGTTTCATTCGAAGTCATTCT---ATAAGTTT---AATTCACTTACTTTCTTACTCAGGAGATAATGTTTAGAAG----GAAA--TAGCATTT-GCACTTTCAC--AAATCACC--TCCTACGCTCTTACAGCCCCGAAACGCACTGTCCTCG---GG----------TCGTCACATACTCCCAAAA----------GAGATTTG-GTT--TAGGTTTCTTTT--T-------------TTTTTTGTTCATAATGATTTAATTAGAATTCGTAAGASizeHit CountTotal NormTotal
No Reads
droAna3
TA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTAGTAATCGTAAGTSizeHit CountTotal NormTotalM044
Female-body		M058
Embryo		V039
Embryo		V055
Head		V105
Male-body		V106
Head
No Reads
droBip1
ATTTATTG--TTTT--------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTTTCGATCAAACA---T--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ATAATATATAGTATATTTT---ATGTATATTTAGTAATCGTAAGTSizeHit CountTotal NormTotal
No Reads
dp5
CC---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TACAGCCCTGAAACGCACTGTATATGT-TAGTATGGGGA--CAGCCA---ACGCGCAGTA----------GAAACTT--------------------------------------CTCTGTTA---TAATTTTGTTTTTACATTTAAGASizeHit CountTotal NormTotalM022
Male-body		M040
Female-body		M059
Embryo		M062
Head		V043
Embryo		V051
Head		V112
Male-body		SRR902010
Ovary		SRR902011
Testis		SRR902012
CNS imaginal disc
...........................................................................................................................................................................................................................................................................................................TACAGCCCTGAAACGCACTGTA...............................................................................................................................2211.0010100000000
............................................................................................................................................................................................................................................................................................................................................TGGGGA--CAGCCA---ACGCGCAGTA----------GAAAC..........................................................................2711.0010000000010
....................................................................................................................................................................................................................................................................................................................GAAACGCACTGTATATGT-TAG......................................................................................................................2111.0010100000000
...................................................................................................................................................................................................................................................................................................................................TGT-TAGTATGGGGA--CAGCCA---ACGCGCAG...........................................................................................2811.0010100000000
..........................................................................................................................................................................................................................................................................................................................................TATGGGGA--CAGCCA---ACGCGCAGTA----------...............................................................................2411.0010000001000
.......................................................................................................................................................................................................................................................................................................................................................................................T--------------------------------------CTCTGTTA---TAATTTTGTCG............2040.5020000000020
................................................................................................................................................................................................................................................................................................................................................................CGCAGTA----------GAAACTT--------------------------------------C.................................15180.0610000000010
droPer2
CC---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TACAGCCCTGAAACGCACTGTATATGT-TAGTATGGGGA--CAGC--------CATAGAAACGCGCAGTAGCAAC--------------------------------------TTCTCTGTTA---TAATTTTGTTTGTACATTTAAGASizeHit CountTotal NormTotalM021
Embryo		M042
Female-body		V042
Embryo		V050
Head		V057
Head		V111
Male-body
..................................................................................................................................................................................................................................................................................................................................................................TAGAAACGCGCAGTAGCAAC--------------------------------------TTCT................................2411.001100000
........................................................................................................................................................................................................................................................................................................................................................................CGCGCAGTAGCAAC--------------------------------------TTCTCTGTTA---TAA....................2711.001010000
droMoj3
TGTTT---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TT--TT-------------TTTTCCTTTTC---TTTTTTATATAAAATC-------SizeHit CountTotal NormTotalM046
Female-body		V041
Embryo		V049
Head		V056
Head		M060
Embryo		V110
Male-body
No Reads

Generated: 01/24/2015 at 02:56 AM