dme-mir-9b

This miRNA belongs to a cluster which includes dme-mir-9c, dme-mir-306, dme-mir-79, and dme-mir-9b.
The C substitution in the mature region of the grimshawi ortholog is supported by all the trace reads:
Changes
- The reported simulans ortholog reported in the 12-way alignment is wrong as shown below:

The best ortholog was found in the nc48 strain. Other suboptimal hits were found in c1674, md106, md199, and sim6. No hits found in sim4 or w501

>dme-mir-9b-dm3
TGTTGCTCTTTTGTTTGCATATTATTTGCTCTTTGGTGATTTTAGCTGTATGGTGTTTATGTATATTCCATAGAGCTTTATTACCAAAAACCAAATGGTTTCTGCATTATGTTTGAGTTGA
>vom21g07.g1:730-849
TGTTGCTCTTTTGTTTGCATATTATTTGCTCTTTGGTGATTTTAGCTGTATGGTGTATAAGT--ATTCCATAGAGCTTTATTACCAAAAACCAAATGGTTTCTGCATTATGCTTGAGTTGA

Corrected alignment

dme-mir-9b

Species	Coordinate	Alignment
dm3	chr2L:16698729-16698849 +	TGTTGCTCTT------------T-TGTTTGCATATTATTTGCTCTTTGGTGATTTTAGCTGTATGGTGTTTATGTATATTCCATAGAGCTTTATTACCAAAAACCAAATGGTTTCTGCATTAT---GTTTGAGTTGA
droSim1	vom21g07.g1:730-849 +	TGTTGCTCTT------------T-TGTTTGCATATTATTTGCTCTTTGGTGATTTTAGCTGTATGGTGTATA--AGTATTCCATAGAGCTTTATTACCAAAAACCAAATGGTTTCTGCATTAT---GCTTGAGTTGA
droSec1	super_7:345741-345859 +	TGTTGCTCTT------------T-TGTTTGCATATTATTTGGTCTTTGGTGATTTTAGCTGTATGGTGTATA--AGTATTCCATAGAGCTTTATTACCAAAAACCAAATGGTTTCTGCATTAT---GCTTGAGTTGA
droYak2	chr2R:4404225-4404345 -	GGTTGCTCTT------------T-TGTTTGCATATTATTTGCTCTTTGGTGATTTTAGCTGTATGGTGTGTTTATGTGTTCCATAGAGCTTTATTACCAAAAACCAAATGGTTTCTGCATTAT---GCTTGAGTTGA
droEre2	scaffold_4845:19768375-19768493 +	AGTTGCTCTT------------T-TGTTTGCATATTATTTGCTCTTTGGTGATTTTAGCTGTATGGTGTGTA--TGTGTTCCATAGAGCTTTATTACCAAAAACCAAATGGTTTTTGCATTAT---GCTTGAGTTGA
droAna3	scaffold_12916:15864526-15864643 -	AGTTGCTCTT------------T-TGTTTGCACATTATTTGTTCTTTGGTGATTTTAGCTGTATGGTGTCT---CATATTCCATAGAGCTTTATTACCAAAAACCAAATGGTTTTTGCATTTT---TTCTGAGTTGC
dp4	chr4_group3:8444179-8444302 +	AGTTGATCTT------------T-TGTTTGCACATTATTTGATCTTTGGTGATTTTAGCTGTATGGTGTATATCAAAGATCCATAGAGCTTTATCACCAAAACCCAAATGGTTTTTGCATTTTTTCGCTTGAGTTGC
droPer1	super_1:9895298-9895421 +	AGTTGATCTT------------T-TGTTTGCACATTATTTGATCTTTGGTGATTTTAGCTGTATGGTGTATATCAAAGATCCATAGAGCTTTATCACCAAAACCCAAATGGTTTTTGCATTTTTTCGCTTGAGTTGC
droWil1	scaffold_181132:873333-873452 -	ATTGATTTTG------------A-TTTTCGCATATTATTTGGTCTTTGGTGATTTTAGCTGTATGGTGTATGAGCATAATCCATAGAGCTTTATTACCAAAAACCAAATGGTTTTTGCATTTT---GCTTGAGTTG-
droVir3	scaffold_12963:12862857-12862965 -	TGTTGCCTTT------------TTATTTTGCATATTATTTGCTCTTTGGTGATTTTAGCTGTATGGTGTGCG--CGTATTCCATAGAGCTTTATTACCAAAAACCAAATGGTTTTTGCATT--------------GC
droMoj3	scaffold_6500:7202293-7202399 +	TG--GCTTTT------------T-ATTTTGCATATTATTTGTTCTTTGGTGATTTTAGCTGTATGGTGTGCG--CGTATTCCATAGAGCTTTATTACCAAAAACCAAATGGTCTTTGCATT--------------GC
droGri2	scaffold_14978:831270-831399 +	TGCTGCCCTTTGTTTGCCCTTTTGAGTTTGCATATTATTTGCTCTTTGGTGATTTTAGCTCTATGGTGTGCG--CATAATCCACAGAGCTTTATTACCAAAAACCAAATGGTTTTTGCATT-T---GCTTTCTTTT-