dme-mir-4963
Changes
| Species | Chrom | Pos | Ref base | Type | A | C | G | T | RNAseq Total | A | C | G | T | Trace Total | Decision | Partition |
| droSim1 | chrX | 4425329 | G | 3 | 0 | 0 | 0 | 0 | 0 | 0.556 | 0 | 0.444 | 0 | 9 | Ambiguous - Suggested base change does not agree with neighboring species | Loop |
| droSim1 | chrX | 4425330 | C | 3 | 0 | 0 | 0 | 0 | 0 | 0.333 | 0.444 | 0.222 | 0 | 9 | Ambiguous - Suggested base change does not agree with neighboring species | Loop |
| droSim1 | chrX | 4425340 | A | 3 | 0 | 0 | 0 | 0 | 0 | 0.333 | 0 | 0 | 0.667 | 9 | Ambiguous- flagged as a genomic error but reseqencing confirmed the genomic base accuracy | miR |
| droSim1 | chrX | 4425348 | G | 3 | 0 | 0 | 0 | 0 | 0 | 0.556 | 0 | 0.444 | 0 | 9 | Ambiguous - Suggested base change does not agree with neighboring species | miR |
Corrected alignment
| Species | Coordinate | Alignment |
|---|---|---|
| dm3 | chrX:5684770-5684909 + | CACCCACTGCGAATTGCTGTAGGAAAT---------------------------------------------------------------------TTTTTGGGGTACTTCTGG-TTTCATAACGCAGAGAATCAT----------CAGCGTGAAG-----------GAATGATTCTCTGCGGGCTGGAACCAGAAGTCCTGCAAATGTTCTT-----TTACGGAT-------------------------------------------TTTAA----CCA---G---------------G-- |
| droSim1 | chrX:4425250-4425396 + | CACCCACTGCGAATTGCTATAGGAAATG------------------------------------ATGG-------------------------AG-TTTTTGGGGTACTTCTGG-TTTCATACTGCAGAGAATCTT----------CAGCGTGCAG-----------TAATGATTTCCTGCGGGCTGCAACCAGAAGTCCTGCAGATGCTCTT-----TTACGGAT-------------------------------------------TTTAA----CCA---G---------------G-- |
| droSec1 | super_4:1079282-1079434 - | CACCCACTGCGAATTGCTATAGGAAATG------------------------------------ATGG-------------------------AG-TTTTTGGGGTACTTCTGG-TTTCATAACTCAGAGAATCTTCATGA---------------TTTCTTAATCATAATGATTTCCTGCGGGCTGCAACCAGAAGTCCTGCAGATGCTCTT-----TTACGGAT-------------------------------------------TTTAA----CCA---G---------------G-- |
| droYak2 | chrX:2788911-2789034 - | CACCCACTGCGAAATGCTATAGGACATG------------------------------------ATGG-------------------------AGCTTCTGGAGGTACTTCTGG-TTTTATAATGCAGAGAATCGT----------AATCGTGAAG-----------GAATGATTCTATGCGTACTGAAATCGGGAGTCCTGCAGAT------------------------------------------------------------------------------------------------ |
| droEre2 | scaffold_4690:3033374-3033523 + | CACCCACTGCGAAATGCTATAGGAAACG------------------------------------ATGG-------------------------GG-TTTCTGGGGTACTTCTGG-TTTTATAACGCACGGAATCTG----------AATCGTGAAT-----------GAATGATTCCTTGCGGACTGGAACCAGAAGTCCTGCAGAGGCTTCT-----TGAATGAT-------------------------------------------TTGAAGTT-TCG---G---------------A-- |
| droAna3 | scaffold_13117:1117754-1117783 + | CACCCACTGCGACGTACTTTAGAGAGTT------------------------------------GT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- |
| dp4 | chr3:13862643-13862665 + | CACCAGCTGTGGGCTGCTTTAGG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- |
| droPer1 | super_4:1457976-1457998 - | CACCAACTGTGGGCTGCTTTAGG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- |
| droWil1 | scaffold_181096:7345062-7345085 - | AACCCACTGTGATGTCCTGTAAAA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- |
| droVir3 | scaffold_13324:2924689-2924720 + | CACGGCCTGTGGTGTGCTATAAA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTTATGT-G- |
| droMoj3 | scaffold_6496:13805954-13805976 - | CACCGACTGCGGCGTCCTCTGAG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- |
| droGri2 | scaffold_15112:3786307-3786359 + | CACCAGCTGCGGCGTGCTATAAAT---------------------------------------------------------------------TC-TTTTCGTTGTATTTCTGG-GTGT------------------------------------------------------------------------------------------------------------------------------------------------------------------TCA---------------A--C |