dme-mir-306

This miRNA belongs to a cluster which includes dme-mir-9c, dme-mir-306, dme-mir-79, and dme-mir-9b.
Changes
- The reported simulans ortholog reported in the 12-way alignment is wrong as shown below:

The correct ortholog was found in its entirety in 6 of the 7 strains, and partially found in md106.

>dme-mir-306-dm3
GTGAATAGTTTAAAAGTCCACTCGATGGCTCAGGTACTTAGTGACTCTCAATGCTTTTGACATTTTGGGGGTCACTCTGTGCCTGTGCTGCCAGTGGGACATAATCTACAAATAA
>zdv96e12.b1:525-640
GTGAATAGTTTAAAAGTCCACTCGATGGCTCAGGTACTTAGTGACTCTCAATGCCTTTGACATTTTGGGGGTCACTCTGTGCCTGTGCTGCCAGTGGGACATAATCTACAAATAA

This miRNA lie in the same intron of the grp gene as 79 and 9b, and one intron downstream of 9c. The genome browser net track shows that this region's alignment with simulans is ambiguous:

Flybase also does not report an ortholog for grp in simulans, however one exists for sechelia (GM17194).

Corrected alignment

dme-mir-306

Species	Coordinate	Alignment
dm3	chr2L:16698389-16698503 +	GTGAATAG-TTTAAA------AG--------------TCCACTC-G-----ATGGCTCAGGTACTTAGTGACTCTCAAT--------GCTTTTGA--CATTTTGGGGGTCACTCTGTGCCTGTGCTG-----CCAGTGGGACATA-ATCTACA----AATA-------------A
droSim1	zdv96e12.b1:525-640 +	GTGAATAG-TTTAAA------AG--------------TCCACTC-G-----ATGGCTCAGGTACTTAGTGACTCTCAAT--------GCCTTTGA--CATTTTGGGGGTCACTCTGTGCCTGTGCTG-----CCAGTGGGACATA-ATCTACA----AATA-------------A
droSec1	super_7:345406-345520 +	GTGAACAG-TTTAAA------AT--------------TCCACTC-G-----ATGGCTCAGGTACTTAGTGACTCTCAAT--------GCCTTTGA--CATTTTGGGGGTCACTCTGTGCCTGTGCTG-----CCAGTGGGACATA-ATCTACA----AATA-------------A
droYak2	chr2R:4404548-4404662 -	GTGAAAAG-TTTTAA------AG--------------TCCACTC-G-----GTGGCTCAGGTACTTAGTGACTCTCAAT--------GCTTTTGA--CATTTTGGGGGTCACTCTGTGCCTGTGCTG-----CCAGTAGGACATA-ATCCACA----AATA-------------A
droEre2	scaffold_4845:19768040-19768155 +	GTGAATAG-TTTAAG------TG--------------TCCACTC-G-----GTGGCTCAGGTACTTAGTGACTCTCAAT--------GCTTTTGA--CATTTTGGGGGTCACTCTGTGCCTGTGCTG-----CCAGTGGGACGCA-ATCAACA---AAATA-------------A
droAna3	scaffold_12916:15864848-15864962 -	GTGAAAGT-GCCAGA------AG--------------TCCACTT-G-----GTAGCTCAGGTACTTAGTGACTCTCAAT--------GCATTTGA--CATTTTGGGAGCCACTTTGTGCCTGGGCCA-----TCAGTGCGACTAT-ATCTTCT----ATTA-------------A
dp4	chr4_group3:8443802-8443917 +	GTGATTTA-TCTACA------AG--------------CACACTTTG-----CCGGCTCAGGTACTTAGTGACTCTCAAT--------GCTTTCGA--CATCTTGAGAGTCTCTCTGTGCCTGCACTG-----GCGGTGGTGCAAC-TGCAACG----ATTA-------------A
droPer1	super_1:9894921-9895036 +	GTGATTTA-TCTACA------AG--------------CACACTTTG-----CCGGCTCAGGTACTTAGTGACTCTCAAT--------GCTTTCGA--CATCTTGAGAGTCTCTCTGTGCCTGCACTG-----GCGGTGGTGCAAC-TGCAACG----ATTA-------------A
droWil1	scaffold_181132:873705-873839 -	GTGACTATACAAAGTGATACGAG--------------TTATCTCTGCCTAAACAGCTCAGGTACTTAGTGACTCTCAAA--------GAATATATATAGACTTGAGAGACGCTCTGTACCTAGACTGTTGTGTCTGCGGAATGTC-AACATCA----TGTG-------------T
droVir3	scaffold_12963:12863192-12863320 -	GTGCTG--------A------ACTC-CTCCCGCCCTGCACACCC-C-----GCTGCTCAGGTACTTAGTGACTCTCAATCATCTATTGCTTTGCA--TACCTTGAGGGTCGCTCTGTGCCTGTGCCG-----CCTGTGCTGCACA-ACCAACG----ACGA-------------G
droMoj3	scaffold_6500:7201931-7202062 +	ATTCATGA-TGTCC-------AG--------------CACACGC-T-----GCAGCTCAGGTACTTAGTGACTCTCAATCACATATTGCTTTGCA--TACTTTGAGGGTCGCTCTGTGCCTGTGCCG-----CCCGTGTTGCACACAGCCACA----CA---ATTGTGA-ATTCG
droGri2	scaffold_14978:830883-831041 +	GTGTTAAA-TGTGGAAAAACCAATCCAATCCG--CTGCACACGT-T-----GCTGCTCAGGTACTTAGTGACTCTCAATTCAACATTGTATTGCA-CGCCTTTGAGGGTCGCTCTGTGCCTGTGTTG-----CACGTGTTGCATT-CACACCATCCGAGTGATTTGTGTCCTTTG