dme-mir-2500
Changes
| Species | Chrom | Pos | Ref base | Type | A | C | G | T | RNAseq Total | A | C | G | T | Trace Total | Decision | Partition |
| droGri2 | scaffold_15110 | 9071783 | G | 3 | 0 | 0 | 0 | 0 | 0 | 0.556 | 0 | 0.444 | 0 | 18 | Genomic Error | Loop |
| droGri2 | scaffold_15110 | 9071791 | G | 3 | 0 | 0 | 0 | 0 | 0 | 0.556 | 0 | 0.444 | 0 | 18 | Genomic Error | miR* |
The reported persimilis ortholog reported in the 12-way alignment is wrong as shown above. Here is the correct ortholog found from the persimilis trace reads.
>dme-mir-2500-dp4
AAGAACTTTTTGCAGGTAAGCCATCACCAGCATTGAAAGCCATTGAAATGCGTCTAAATAGCAGTCTCTCTCCTCCCACAGATCATGTTCCAGGCC
>gnl|ti|780630171:515-611
AAGAACTTTTTGCAGGTAAGCCATCACCAGCATTGAAAGCCATTGAAATGCGTCTAAATAGCAGTCTCTCTCCTCCCACAGATCATGTTCCAGGCC
Corrected alignment
| Species | Coordinate | Alignment |
|---|---|---|
| dm3 | chr3L:16991543-16991639 - | AAGAACTTTCTGCAGGTAAGGAA------CACAC------------------ATATAC--------------------------AATACCGTTTGA-------------------------A-----------------------TTTCAAATAATCAGGAT-----------TTTGTGTGTGGA-----C-CTCAGATCATGTTCCAGGCC |
| droSim1 | chr3L:16333274-16333368 - | AAGAACTTTCTGCAGGTAAGGAA------CACAC------------------ATAC----------------------------AGTCCCTGTTGA-------------------------A-----------------------TTTCAAATAATCAGGAT-----------TTTGTGTGTGGA-----C-CTCAGATCATGTTCCAGGCC |
| droSec1 | super_0:9052557-9052651 - | AAGAACTTTCTGCAGGTAAGGAA------CACAC------------------ATAC----------------------------AATCCCTGTTGA-------------------------A-----------------------TATTAAATAATCAGGAT-----------TTTGTGTGTGGA-----C-CTCAGATCATGTTCCAGGCC |
| droYak2 | chr3L:17082640-17082734 + | AAGAACTTTCTACAGGTCAGGAT------CACAC------------------ATAC----------------------------ATTCCCTGTAGA-------------------------A-----------------------TTTAATTTAATCAGGAA-----------TTTATGTGTGAA-----C-TTTAGATCATGTTCCAGGCC |
| droEre2 | scaffold_4784:16851458-16851552 + | AAGAATTTTCTGCAGGTAAGGAT------CGCAC------------------ATAC----------------------------AATCCCTGTCGC-------------------------A-----------------------TTTTATGTAATCAGGAT-----------TTTGCGTGTGAA-----C-TTTAGATCATGTTCCAGGCC |
| droAna3 | scaffold_13337:13625775-13625865 + | AAAAACTTTCTGCAGGTGAGCAG------TTCA------------------------------------------------------------------------AGCTTAGCGAG------GTCTGT-TCCAGTTTCCTAACAT---------------------TC----T----TC------CGCTCC-TTTAGATCATGTTCCAGGCC |
| dp4 | chrXR_group8:4827167-4827262 - | AAGAACTTTTTGCAGGTAAGCCATCACC------A-----G-------------------------------------CATTGA---------------------AAGCCATTGA------AATGCG-----------------------TCTAAATAGCAG-----------TCTCTCTC------CTCC-CACAGATCATGTTCCAGGCC |
| droPer1 | gnl|ti|780630171:515-611 - | AAGAACTTTTTGCAGGTAAGCCATCACC------A-----G-------------------------------------CATTGA---------------------AAGCCATTGA------AATGCG-----------------------TCTAAATAGCAG-----------TCTCTCTC------CTCC-CACAGATCATGTTCCAGGCC |
| droWil1 | scaffold_180955:197087-197182 - | AAAAACTTTTTACAGGTAAGCCACAAC------GATGAATG-------------------------------------GCTCGA---------------------GGGAGATTGA------ACTCTA-----------------------TGTA--------ATCTCTTCA--------------CTTTCT-TGCAGATAATGTTTCAGGCT |
| droVir3 | scaffold_13049:10166522-10166615 + | AAGAACTTTCTGCAGGTGCGTTCCCCCT------G-----G-CTGATCACATATGT----------------------------------------CGTTTATAC---------------------------------TATAT--------------------TTT-CG-CCTTT----------TGCTCC-TGCAGATCATGTTCCAGGCC |
| droMoj3 | scaffold_6680:10629193-10629280 - | AAGAACTTTCTCCAGGTGAGTTACGG---------------T----TC----ACAT----------------------------AATTC---TCGT-------------------------A-----------------------TTCCAAATAACGAA--A-----------TCTTTG--------TCCT-TGCAGATCATGTTCCAGGCC |
| droGri2 | scaffold_15110:9071735-9071831 - | AAGAACTTGCTGCAGGTGAGATTTT---G------------ATCGATTAATT-----------A---------------------------------------------------AGTCTG------GCTCTCTTTGCTAAATAT---------------------GC-CAC-TTTTTG--------TGTT-GGCAGATCATGTTCCAGGCC |